FastQCFastQC Report
Fri 27 May 2016
SRR2139287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5751273
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96800.052.231451
GTATCAA180050.038.739121
ATCAACG225050.030.4802653
TCAACGC225150.030.4282344
TATCAAC228250.030.258912
CAACGCA229500.029.7769995
AACGCAG232600.029.4903896
CGCAGAG258400.026.5139878
ACGCAGA265750.025.7956357
GTGGTAT46350.024.6197851
TGGTATC45800.024.5421712
CTTATAC34100.024.44011
GCAGAGT278000.023.9582029
GAGTACT171150.022.64387912-13
AGAGTAC244250.021.972810-11
GTACATG179300.021.715051
TATACAC51200.021.6198433
CAGAGTA264400.021.55489710-11
TACATGG174950.021.4976712
AGTACTT186700.020.10581812-13
Produced by FastQC (version 0.11.5)