##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139287_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5751273
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65711121694275	33.0	33.0	33.0	33.0	33.0
2	32.67603867874121	33.0	33.0	33.0	33.0	33.0
3	32.66387632790167	33.0	33.0	33.0	33.0	33.0
4	36.591331866875386	37.0	37.0	37.0	37.0	37.0
5	36.613404023770045	37.0	37.0	37.0	37.0	37.0
6	36.57069869574962	37.0	37.0	37.0	37.0	37.0
7	36.54164912707152	37.0	37.0	37.0	37.0	37.0
8	36.54853056010382	37.0	37.0	37.0	37.0	37.0
9	36.562291513548395	37.0	37.0	37.0	37.0	37.0
10-11	36.50471182640783	37.0	37.0	37.0	37.0	37.0
12-13	36.474767064613346	37.0	37.0	37.0	37.0	37.0
14-15	35.02911320676309	40.0	35.0	40.0	19.5	40.0
16-17	35.3065919492954	40.0	33.0	40.0	24.0	40.0
18-19	37.937583557588034	40.0	37.0	40.0	35.0	40.0
20-21	38.313167882777556	40.0	37.0	40.0	37.0	40.0
22-23	38.36438384479494	40.0	37.0	40.0	37.0	40.0
24-25	38.403768323533725	40.0	37.0	40.0	37.0	40.0
26-27	38.33834939822658	40.0	37.0	40.0	37.0	40.0
28-29	38.264612073293705	40.0	37.0	40.0	37.0	40.0
30-31	38.13162949957999	40.0	37.0	40.0	37.0	40.0
32-33	37.93057155460029	40.0	37.0	40.0	33.0	40.0
34-35	37.84099234661426	40.0	37.0	40.0	33.0	40.0
36-37	37.763562280231056	40.0	37.0	40.0	33.0	40.0
38-39	37.77426993256637	40.0	37.0	40.0	33.0	40.0
40-41	37.72112813712273	40.0	37.0	40.0	33.0	40.0
42-43	37.6801520277747	40.0	37.0	40.0	33.0	40.0
44-45	37.624095666720265	40.0	37.0	40.0	33.0	40.0
46-47	37.472887568096255	40.0	37.0	40.0	33.0	40.0
48-49	37.23822367968592	40.0	37.0	40.0	33.0	40.0
50-51	37.15623272085405	40.0	37.0	40.0	33.0	40.0
52-53	37.082172528759656	38.5	37.0	40.0	33.0	40.0
54-55	36.85654930200348	37.0	37.0	40.0	33.0	40.0
56-57	36.755264344588426	37.0	37.0	40.0	33.0	40.0
58-59	36.706457821091455	37.0	37.0	40.0	33.0	40.0
60-61	36.59330644791428	37.0	37.0	40.0	33.0	40.0
62-63	36.41054650487499	37.0	37.0	40.0	33.0	40.0
64-65	36.1392018730912	37.0	37.0	40.0	33.0	40.0
66-67	35.79960845495896	37.0	33.0	40.0	33.0	40.0
68-69	35.504078912561994	37.0	33.0	37.0	30.0	40.0
70-71	35.267968597977486	37.0	33.0	37.0	27.0	40.0
72-73	35.05238088073806	37.0	33.0	37.0	27.0	40.0
74-75	34.758953193716124	37.0	33.0	37.0	27.0	40.0
76-77	31.810697781041675	33.0	30.0	35.0	24.5	37.0
78-79	33.99350268169465	37.0	33.0	37.0	27.0	37.0
80-81	34.33790780156427	37.0	33.0	37.0	27.0	37.0
82-83	34.3237177635469	37.0	33.0	37.0	27.0	37.0
84-85	34.27958087768877	37.0	33.0	37.0	27.0	37.0
86-87	34.08870671294761	37.0	33.0	37.0	27.0	37.0
88-89	33.848583832646725	37.0	33.0	37.0	27.0	37.0
90-91	33.68881657392372	35.0	33.0	37.0	27.0	37.0
92-93	33.7065024272794	33.0	33.0	37.0	27.0	37.0
94-95	33.63413601139869	33.0	33.0	37.0	27.0	37.0
96-97	33.5785952187827	33.0	33.0	37.0	27.0	37.0
98-99	33.590460403876804	33.0	33.0	37.0	27.0	37.0
100	33.63397034594753	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	8.0
20	43.0
21	247.0
22	916.0
23	2706.0
24	6510.0
25	12938.0
26	23201.0
27	37673.0
28	56729.0
29	80347.0
30	109701.0
31	147518.0
32	197002.0
33	268823.0
34	378637.0
35	585419.0
36	1030817.0
37	1745165.0
38	1042937.0
39	23934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.772485341930626	20.372703832841065	14.787971141673257	23.066839683555056
2	14.698349976379053	21.077153474957015	41.61120771811211	22.613288830551824
3	18.068493846188087	26.47232801597988	30.650285017192836	24.808893120639194
4	12.533901325798738	18.40125190176049	38.76612910236905	30.298717670071724
5	13.35868890129968	37.74385057347354	35.27368225580863	13.623778269418152
6	29.516161030783966	37.98579897007149	18.777894911265733	13.720145087878805
7	26.146993891613214	33.20930861741392	22.8674243076272	17.77627318334567
8	24.58753044760699	36.320793674722104	21.065892716273424	18.025783161397484
9	25.535007640917062	17.21156342256749	21.352281486203143	35.9011474503123
10-11	24.198364431665826	27.100721874965767	28.467558399679515	20.23335529368889
12-13	25.17770413611039	24.60826498759492	28.836146362727	21.37788451356769
14-15	22.595880951783588	25.569520846605005	26.883017589437923	24.951580612173487
16-17	21.58367373623196	29.074267557808508	27.393239722753552	21.948818983205978
18-19	21.477245124688046	27.86723043750488	29.722463183368276	20.9330612544388
20-21	22.38633929266963	26.949634288162567	29.701741060995563	20.96228535817225
22-23	22.0750269902529	26.952941311503693	29.57392616357206	21.398105534671345
24-25	21.83430132092096	27.160731057304798	29.65706821152655	21.347899410247688
26-27	21.807570591822774	27.279458605240997	29.58494078255436	21.328030020381867
28-29	21.614202540756946	27.33590523742382	29.633852069177323	21.416040152641916
30-31	22.039704590009375	27.17016594183778	29.485845562876996	21.30428390527585
32-33	21.83089413167723	27.370143500846204	29.35273408447781	21.446228282998756
34-35	21.834286913176836	27.45708628118566	29.386784571131525	21.32184223450598
36-37	21.935384244179033	27.353570449335923	29.27699579947137	21.43404950701368
38-39	21.78051291074649	27.459613777328112	29.30256455373246	21.457308758192937
40-41	22.044852231580926	27.39021056726158	29.094261447349005	21.47067575380849
42-43	22.03663964076559	27.519254514140957	28.824191594049513	21.61991425104394
44-45	22.048314198232752	27.721396262328508	28.57443797696659	21.655851562472154
46-47	22.220587761751993	27.662659438006116	28.09261722078913	22.024135579452764
48-49	22.12328294769011	27.886195051076484	28.02071944690371	21.969802554329696
50-51	22.101679111503017	27.97060713276905	27.92879516846783	21.998918587260107
52-53	22.307975881099075	27.93535747656729	27.75013139837066	22.006535243962976
54-55	22.192346412608856	27.87602420765315	27.792606735967713	22.139022643770282
56-57	22.303940644846207	27.990802666024344	27.6709210576307	22.03433563149875
58-59	22.20049643006929	28.13185433705936	27.69113407242293	21.976515160448415
60-61	22.254430947568622	28.068728509477463	27.682819416229588	21.994021126724327
62-63	22.23155624489531	28.24741407933032	27.55613956902675	21.96489010674762
64-65	22.238189976154672	28.145505944152223	27.563785513738516	22.05251856595459
66-67	22.31573333282174	28.049646495579577	27.47904177544064	22.155578396158045
68-69	22.287759778869106	28.06367790224371	27.554651428934523	22.093910889952664
70-71	22.323152256590127	27.833187356466528	27.550976264444653	22.292684122498695
72-73	22.31957727656354	27.847314753693443	27.51467493784581	22.3184330318972
74-75	22.117280336210317	27.983267502230085	27.53909165417116	22.360360507388435
76-77	22.331553448883053	27.92792494929108	27.456258074729096	22.284263527096773
78-79	22.28603052396491	27.916127529774528	27.474715240408575	22.32312670585199
80-81	22.275868199367448	27.92920253306239	27.512618521953485	22.282310745616673
82-83	22.323839221791278	27.87951776710639	27.536582274297118	22.260060736805222
84-85	22.420669763057294	27.885117929102805	27.504395359168104	22.189816948671798
86-87	22.232772469486147	27.874099065339834	27.538029583599034	22.355098881574992
88-89	22.275660735346793	27.788970131640806	27.532813897643173	22.402555235369228
90-91	22.320799106210966	27.78081556936383	27.601316001948273	22.297069322476933
92-93	22.362072232066268	27.73380424456839	27.570982321459702	22.33314120190564
94-95	22.224504793873376	27.761665470208186	27.61649012992688	22.39733960599156
96-97	22.197956574886206	27.922371003197394	27.74169322619115	22.13797919572525
98-99	22.386864525731703	28.67062534648018	26.778272477019616	22.164237650768506
100	20.4637040188371	28.277042630654798	28.03859990171777	23.220653448790333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1170.0
1	1134.0
2	1771.5
3	2621.5
4	3802.5
5	6469.0
6	8559.0
7	9792.0
8	10652.0
9	10619.5
10	10032.0
11	9460.0
12	10528.5
13	14277.0
14	22682.5
15	33851.0
16	43684.0
17	50294.0
18	50822.5
19	46068.5
20	39706.5
21	33478.0
22	29026.0
23	28299.5
24	31075.0
25	36765.0
26	45578.0
27	55886.5
28	67250.0
29	79991.5
30	92176.0
31	104171.5
32	117257.0
33	129554.5
34	141420.0
35	152811.5
36	166416.0
37	178990.5
38	186036.5
39	191104.5
40	197494.5
41	204467.0
42	209521.0
43	214139.0
44	217435.5
45	220423.5
46	223609.5
47	224438.0
48	222578.0
49	218992.0
50	214996.0
51	209557.0
52	201094.5
53	191415.0
54	181114.0
55	168173.0
56	154436.0
57	139945.0
58	124296.0
59	109065.0
60	92654.0
61	76331.0
62	61065.0
63	47133.0
64	35605.0
65	26971.5
66	20726.5
67	16018.5
68	12273.5
69	8955.5
70	6376.0
71	4716.5
72	3523.0
73	2577.5
74	1881.5
75	1328.0
76	928.5
77	662.0
78	466.0
79	331.5
80	248.5
81	175.0
82	121.5
83	94.5
84	78.5
85	54.5
86	37.0
87	29.0
88	20.0
89	13.5
90	9.5
91	7.0
92	5.0
93	3.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	6.954981966601133E-5
2	3.825240081630623E-4
3	7.128856515766162E-4
4	3.999114630795651E-4
5	8.693727458251417E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.827727878680077
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009446000753766505
26-27	0.02615165855907233
28-29	0.009432096352652757
30-31	6.103940869904005E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.024059300309341596
50-51	0.07124187546118618
52-53	0.06341422400924167
54-55	0.039141309470538906
56-57	0.004721276839813347
58-59	0.00912629002848368
60-61	0.03486382207929219
62-63	0.03602068934806515
64-65	0.0
66-67	0.003395599092550123
68-69	0.03716162275259567
70-71	0.09575589203613416
72-73	0.08655223479604836
74-75	0.04212596149896008
76-77	0.008705797540216477
78-79	6.555834733256196E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0016847971996482494
92-93	0.0030054418533825246
94-95	0.0012030647279467736
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	174.0
20-21	609.0
22-23	1274.0
24-25	2941.0
26-27	3995.0
28-29	5826.0
30-31	10065.0
32-33	10750.0
34-35	13502.0
36-37	15088.0
38-39	16103.0
40-41	22448.0
42-43	29568.0
44-45	34827.0
46-47	35580.0
48-49	28136.0
50-51	30266.0
52-53	25210.0
54-55	30401.0
56-57	38930.0
58-59	37740.0
60-61	48839.0
62-63	33147.0
64-65	45207.0
66-67	56087.0
68-69	35805.0
70-71	54541.0
72-73	47198.0
74-75	39289.0
76-77	26654.0
78-79	53663.0
80-81	48822.0
82-83	57456.0
84-85	69079.0
86-87	76494.0
88-89	75392.0
90-91	78256.0
92-93	83223.0
94-95	99812.0
96-97	222045.0
98-99	45059.0
100-101	4061772.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.77867146298637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.1147966840301	79.97935803544279
2	6.518226809085251	11.443225791918483
3	1.4219342492045286	3.744464979086775
4	0.46887693337235875	1.6462957716425932
5	0.19405987056783647	0.8517158811361888
6	0.09952321500822967	0.5241609355888527
7	0.057111677821467	0.3509231042933334
8	0.038250993492616514	0.26860971127369715
9	0.021650693019101427	0.1710422162522709
>10	0.06363596019809849	0.8220806737866468
>50	0.0012945029218976306	0.07757780248453683
>100	5.817721887122887E-4	0.09203237437329652
>500	5.6639089776181305E-5	0.0285127227205563
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.7387454916502832E-5	0.0	0.0	0.0
3	0.0	1.7387454916502832E-5	0.0	0.0	0.0
4	0.0	8.693727458251417E-5	0.0	0.0	0.0
5	0.0	1.04324729499017E-4	0.0	0.0	0.0
6	0.0	1.04324729499017E-4	0.0	0.0	0.0
7	0.0	1.04324729499017E-4	0.0	0.0	0.0
8	0.0	1.2171218441551983E-4	0.0	0.0	0.0
9	0.0	3.303616434135538E-4	0.0	1.7387454916502832E-5	1.7387454916502832E-5
10-11	0.0	3.303616434135538E-4	0.0	1.7387454916502832E-5	1.7387454916502832E-5
12-13	0.0	3.303616434135538E-4	0.0	1.7387454916502832E-5	1.7387454916502832E-5
14-15	0.0	4.17298917996068E-4	0.0	1.7387454916502832E-5	1.7387454916502832E-5
16-17	0.0	4.346863729125708E-4	0.0	5.2162364749508496E-5	1.7387454916502832E-5
18-19	0.0	4.694612827455765E-4	0.0	7.824354712426275E-5	1.7387454916502832E-5
20-21	0.0	5.303173749533364E-4	0.0	1.1301845695726842E-4	1.7387454916502832E-5
22-23	0.0	5.998671946193478E-4	0.0	1.4779336679027408E-4	1.7387454916502832E-5
24-25	0.0	6.25948376994102E-4	0.0	1.9126200408153116E-4	1.7387454916502832E-5
26-27	0.0	6.433358319106048E-4	0.0	2.5211809628929106E-4	1.7387454916502832E-5
28-29	0.0	6.433358319106048E-4	0.0	8.432915634503874E-4	1.7387454916502832E-5
30-31	0.0	7.30273106493119E-4	0.0	0.001877845130982306	1.7387454916502832E-5
32-33	0.0	7.389668339513704E-4	0.0	0.003738302807048109	1.7387454916502832E-5
34-35	0.0	9.041476556581474E-4	0.0	0.00552051693598965	1.7387454916502832E-5
36-37	0.0	0.0010606347499066728	0.0	0.0076417864358029955	1.7387454916502832E-5
38-39	0.0	0.0010954096597396786	0.0	0.010658509863816237	1.7387454916502832E-5
40-41	0.0	0.001251896753988204	0.0	0.013092753552126635	1.7387454916502832E-5
42-43	0.0	0.0012953653912794612	0.0	0.016283351529304903	1.7387454916502832E-5
44-45	0.0	0.001460546212986238	0.0	0.01924791259256864	1.7387454916502832E-5
46-47	0.0	0.001564870942485255	0.0	0.022360267022622643	1.7387454916502832E-5
48-49	0.0	0.0015909521248600093	0.0	0.02490752916789031	1.7387454916502832E-5
50-51	0.0	0.0018256827662327976	0.0	0.027767765501655023	1.7387454916502832E-5
52-53	0.0	0.0018430702211493003	0.0	0.03287098351964861	1.7387454916502832E-5
54-55	0.0	0.001860457676065803	0.0	0.036296312138199664	1.7387454916502832E-5
56-57	0.0	0.0018952325858988088	0.0	0.041973316168437844	1.7387454916502832E-5
58-59	8.693727458251416E-6	0.0019126200408153115	0.0	0.0472591024630547	1.7387454916502832E-5
60-61	1.7387454916502832E-5	0.0019473949506483173	0.0	0.05066704362668926	3.4774909833005664E-5
62-63	1.7387454916502832E-5	0.0020169447703143285	0.0	0.054283634249321845	3.4774909833005664E-5
64-65	1.7387454916502832E-5	0.002312531503894877	0.0	0.057030852126129294	3.4774909833005664E-5
66-67	1.7387454916502832E-5	0.002321225231353128	0.0	0.060908254572509424	4.3468637291257084E-5
68-69	1.7387454916502832E-5	0.0024081625059356425	0.0	0.06500300020534584	5.21623647495085E-5
70-71	1.7387454916502832E-5	0.0024516311432268996	0.0	0.06784584908419405	6.0856092207759916E-5
72-73	1.7387454916502832E-5	0.0024516311432268996	0.0	0.0709147348769568	8.693727458251417E-5
74-75	1.7387454916502832E-5	0.002521180962892911	0.0	0.07354893429680698	8.693727458251417E-5
76-77	1.7387454916502832E-5	0.002668974329683185	0.0	0.07541808570033104	8.693727458251417E-5
78-79	2.6081182374754248E-5	0.0027211366944326935	0.0	0.0764439455404047	8.693727458251417E-5
80-81	3.4774909833005664E-5	0.002738524149349196	0.0	0.07692210055060852	8.693727458251417E-5
82-83	3.4774909833005664E-5	0.0027472178768074475	0.0	0.07742633674318712	9.563100204076558E-5
84-85	3.4774909833005664E-5	0.0027646053317239507	0.0	0.07805228512018123	1.04324729499017E-4
86-87	3.4774909833005664E-5	0.002799380241556956	0.0	0.07859998995005105	1.04324729499017E-4
88	3.4774909833005664E-5	0.0028515426063064645	0.0	0.07867823349717532	1.04324729499017E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9680	0.0	52.23145	1
GTATCAA	18005	0.0	38.73912	1
ATCAACG	22505	0.0	30.480265	3
TCAACGC	22515	0.0	30.428234	4
TATCAAC	22825	0.0	30.25891	2
CAACGCA	22950	0.0	29.776999	5
AACGCAG	23260	0.0	29.490389	6
CGCAGAG	25840	0.0	26.513987	8
ACGCAGA	26575	0.0	25.795635	7
GTGGTAT	4635	0.0	24.619785	1
TGGTATC	4580	0.0	24.542171	2
CTTATAC	3410	0.0	24.4401	1
GCAGAGT	27800	0.0	23.958202	9
GAGTACT	17115	0.0	22.643879	12-13
AGAGTAC	24425	0.0	21.9728	10-11
GTACATG	17930	0.0	21.71505	1
TATACAC	5120	0.0	21.619843	3
CAGAGTA	26440	0.0	21.554897	10-11
TACATGG	17495	0.0	21.497671	2
AGTACTT	18670	0.0	20.105818	12-13
>>END_MODULE