FastQCFastQC Report
Fri 27 May 2016
SRR2139286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865509
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142050.054.4212341
GTATCAA263650.039.6872941
TCAACGC332550.031.0076354
ATCAACG333350.030.8945713
TATCAAC338550.030.6250652
CAACGCA340800.030.1547935
AACGCAG342900.030.0078816
CGCAGAG386700.026.599148
ACGCAGA394300.026.129137
GTGGTAT64100.025.3790381
TGGTATC64650.024.290812
GCAGAGT413400.023.9512869
GAGTACT259250.023.63198912-13
CAGAGTA394650.022.02020610-11
GTACATG255600.021.6736471
TACATGG254500.021.511642
AGAGTAC368350.021.41403810-11
GTACTTT287700.021.27839914-15
AGTACTT276750.020.39617312-13
ACATGGG266450.019.797933
Produced by FastQC (version 0.11.5)