##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139286_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7865509
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69411006967254	33.0	33.0	33.0	33.0	33.0
2	32.70913745060873	33.0	33.0	33.0	33.0	33.0
3	32.69436294586911	33.0	33.0	33.0	33.0	33.0
4	36.6289356480299	37.0	37.0	37.0	37.0	37.0
5	36.649348058720676	37.0	37.0	37.0	37.0	37.0
6	36.610360880649935	37.0	37.0	37.0	37.0	37.0
7	36.584666930010506	37.0	37.0	37.0	37.0	37.0
8	36.59032352515266	37.0	37.0	37.0	37.0	37.0
9	36.59992633661725	37.0	37.0	37.0	37.0	37.0
10-11	36.54985545118568	37.0	37.0	37.0	37.0	37.0
12-13	36.51426716312956	37.0	37.0	37.0	37.0	37.0
14-15	35.48246941170622	40.0	37.0	40.0	19.5	40.0
16-17	35.760332929502724	40.0	37.0	40.0	24.0	40.0
18-19	38.098308386653684	40.0	37.0	40.0	35.0	40.0
20-21	38.436808482732204	40.0	37.0	40.0	37.0	40.0
22-23	38.473783051645086	40.0	37.0	40.0	37.0	40.0
24-25	38.506617594048436	40.0	37.0	40.0	37.0	40.0
26-27	38.445463836249274	40.0	37.0	40.0	37.0	40.0
28-29	38.37372229285843	40.0	37.0	40.0	37.0	40.0
30-31	38.24150985794647	40.0	37.0	40.0	37.0	40.0
32-33	38.047872535114	40.0	37.0	40.0	35.0	40.0
34-35	37.96415179001375	40.0	37.0	40.0	33.0	40.0
36-37	37.89142107105704	40.0	37.0	40.0	33.0	40.0
38-39	37.89511430244852	40.0	37.0	40.0	33.0	40.0
40-41	37.83983061362899	40.0	37.0	40.0	33.0	40.0
42-43	37.78858294812456	40.0	37.0	40.0	33.0	40.0
44-45	37.73708651573217	40.0	37.0	40.0	33.0	40.0
46-47	37.5969304724512	40.0	37.0	40.0	33.0	40.0
48-49	37.37083050032547	40.0	37.0	40.0	33.0	40.0
50-51	37.289551696446736	40.0	37.0	40.0	33.0	40.0
52-53	37.215358598738945	40.0	37.0	40.0	33.0	40.0
54-55	36.99939548485507	37.0	37.0	40.0	33.0	40.0
56-57	36.90012812238254	37.0	37.0	40.0	33.0	40.0
58-59	36.85497161855767	37.0	37.0	40.0	33.0	40.0
60-61	36.74256216464214	37.0	37.0	40.0	33.0	40.0
62-63	36.56614412611325	37.0	37.0	40.0	33.0	40.0
64-65	36.300787454823976	37.0	37.0	40.0	33.0	40.0
66-67	35.974254432946545	37.0	35.0	40.0	33.0	40.0
68-69	35.689272742045915	37.0	33.0	38.5	33.0	40.0
70-71	35.45363663854076	37.0	33.0	37.0	33.0	40.0
72-73	35.2425283427625	37.0	33.0	37.0	33.0	40.0
74-75	34.96437489269164	37.0	33.0	37.0	27.0	40.0
76-77	32.06463302079275	33.0	30.0	35.0	24.5	37.0
78-79	34.21504366430915	37.0	33.0	37.0	27.0	37.0
80-81	34.547049922508606	37.0	33.0	37.0	27.0	37.0
82-83	34.523397421811765	37.0	33.0	37.0	27.0	37.0
84-85	34.48119542868098	37.0	33.0	37.0	30.0	37.0
86-87	34.30201488076685	37.0	33.0	37.0	27.0	37.0
88-89	34.07775448944794	37.0	33.0	37.0	27.0	37.0
90-91	33.92179759953072	37.0	33.0	37.0	27.0	37.0
92-93	33.94056337266061	37.0	33.0	37.0	27.0	37.0
94-95	33.87606735822244	37.0	33.0	37.0	27.0	37.0
96-97	33.81696193472443	33.0	33.0	37.0	27.0	37.0
98-99	33.81131828603344	33.0	33.0	37.0	27.0	37.0
100	33.85430378836153	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	9.0
20	55.0
21	283.0
22	1104.0
23	3286.0
24	7776.0
25	15877.0
26	28563.0
27	46928.0
28	70541.0
29	100930.0
30	138765.0
31	185690.0
32	251542.0
33	342263.0
34	485670.0
35	759675.0
36	1361933.0
37	2478137.0
38	1565298.0
39	21181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.21305957440262	20.14554938529725	14.768325864225698	22.873065176074427
2	14.730683265822977	21.18636417959026	41.43484297167616	22.6481095829106
3	18.124976240729755	26.510857557405892	30.696710165890877	24.667456035973473
4	12.525799475428679	18.362831464616864	38.85836447154461	30.253004588409848
5	13.320750116044708	37.701593909505846	35.23053770369168	13.747118270757763
6	29.405089994811522	38.03115602563038	18.749517672664286	13.814236306893807
7	26.036878223647065	33.36305380872363	22.80528825280093	17.794779714828373
8	24.658582171859443	36.08159370232747	21.10689848552713	18.152925640285964
9	25.652084308847655	17.02590385441044	21.329414282025486	35.99259755471642
10-11	24.11322013616665	27.06963401859943	28.44571788043215	20.371427964801768
12-13	25.232677249495232	24.692890186763503	28.79802820135353	21.276404362387737
14-15	22.60790715908481	25.618483145856196	26.98965301206961	24.783956682989388
16-17	21.426750640041224	29.115185043968545	27.467815496746617	21.99024881924361
18-19	21.392766825389174	27.766543779938463	29.952943922637427	20.887745472034933
20-21	22.307099084762793	26.89243310289183	29.982691299223106	20.817776513122276
22-23	22.011117092509068	26.84473868372948	29.860793788659784	21.283350435101667
24-25	21.735100811233657	27.04030776489763	29.966063910371528	21.258527513497185
26-27	21.702295516567613	27.178603237392302	29.870245108698146	21.24885613734194
28-29	21.53991883988264	27.20353616399347	29.897751193722204	21.358793802401685
30-31	21.93785573878597	27.056069934383604	29.77192172034993	21.234152606480496
32-33	21.704490400800953	27.25619794286412	29.667394344571697	21.37191731176323
34-35	21.753376237424874	27.31283347025811	29.632150662376	21.301639629941015
36-37	21.868615287863797	27.270668449705333	29.499992562122944	21.36072370030793
38-39	21.700591688059625	27.388627271231957	29.499313233564028	21.411467807144387
40-41	21.9790828972353	27.273670940381173	29.296516337146617	21.450729825236913
42-43	21.906958865958174	27.422761441511096	29.067207796285103	21.60307189624563
44-45	22.016746761638263	27.624548857694048	28.733075065745382	21.625629314922303
46-47	22.186190165958255	27.620218747242586	28.222612115467893	21.970978971331263
48-49	22.059092385439747	27.863912147041315	28.101726287425155	21.97526918009378
50-51	22.06449518057575	28.01572606291901	27.9680979198455	21.951680836659733
52-53	22.268437141944002	27.855543139274747	27.838474648550026	22.03754507023123
54-55	22.219190140313614	27.81592364895763	27.89386559518795	22.0710206155408
56-57	22.246891027325553	28.011356413635475	27.72634243682677	22.015410122212202
58-59	22.134304923474634	28.08956680997026	27.76329239051072	22.01283587604438
60-61	22.231211547934457	28.0506498328092	27.811780567264687	21.906358051991656
62-63	22.20439345856494	28.22781732716877	27.649447166852582	21.91834204741371
64-65	22.22180089743095	28.128094865825236	27.633848886309437	22.01625535043438
66-67	22.290724537998287	28.057136227536013	27.540447664004887	22.111691570460817
68-69	22.212046666455898	28.03914897101849	27.645489362834102	22.103314999691506
70-71	22.287368049054106	27.772002214958114	27.667306566804417	22.273323169183364
72-73	22.315199933530348	27.849650435819818	27.57092149891881	22.26422813173102
74-75	22.08568343650491	28.021482399903576	27.581770008958074	22.31106415463344
76-77	22.257278898616025	27.928775673414425	27.531786808563226	22.282158619406324
78-79	22.26975055130951	27.934116417475312	27.458306716525627	22.337826314689547
80-81	22.238605683185888	27.93195863257197	27.48629765603221	22.34313802820993
82-83	22.236394132278026	27.890166681679894	27.580451699534038	22.292987486508036
84-85	22.409841207344947	27.816478457083154	27.541045509590884	22.23263482598102
86-87	22.18795015345588	27.869158686995593	27.602502275963992	22.340388883584534
88-89	22.277213251649407	27.809185916121987	27.598936389177776	22.314664443050834
90-91	22.365576992637475	27.758757231905168	27.6252633915841	22.250402383873258
92-93	22.315831591877817	27.73326908533398	27.626901799813254	22.323997522974953
94-95	22.233497160047936	27.73256964750082	27.65622591211216	22.37770728033908
96-97	22.281427100490387	27.866479907473103	27.716699836007397	22.135393156029114
98-99	22.41885353534838	28.679472272781563	26.783913861176245	22.117760330693812
100	20.562601492322553	28.248850610153003	28.043090985083623	23.145456912440817
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1968.0
1	1835.0
2	2620.0
3	3824.0
4	5449.5
5	9179.5
6	12360.0
7	14592.0
8	16472.0
9	16775.5
10	15733.5
11	14396.0
12	15459.5
13	20677.0
14	33061.0
15	49853.5
16	64454.5
17	74160.0
18	74749.5
19	67402.0
20	57973.5
21	48795.0
22	41758.5
23	39935.5
24	42950.5
25	50258.5
26	61851.0
27	75375.5
28	90560.5
29	108063.0
30	124166.5
31	139562.0
32	157516.5
33	175549.5
34	191832.5
35	205466.0
36	222912.5
37	238780.0
38	247454.5
39	254529.0
40	262766.5
41	271487.5
42	276606.5
43	282029.5
44	287638.0
45	292476.5
46	295850.0
47	294529.0
48	292379.5
49	289435.5
50	284625.0
51	278687.5
52	268998.0
53	256827.0
54	243941.5
55	227193.5
56	207847.5
57	187316.0
58	167014.5
59	147696.5
60	126466.5
61	105045.5
62	83677.0
63	64392.0
64	48830.0
65	36882.0
66	28745.5
67	22929.5
68	18153.0
69	13280.0
70	9342.0
71	6967.5
72	5070.0
73	3631.5
74	2666.5
75	1921.0
76	1373.5
77	980.5
78	676.5
79	472.0
80	337.5
81	233.5
82	183.5
83	140.5
84	96.0
85	76.0
86	57.0
87	39.0
88	29.0
89	22.5
90	17.5
91	15.5
92	13.0
93	6.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	6.229730332773123E-4
3	0.0020087701889350074
4	4.958356795472486E-4
5	7.628241223803825E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.006050085251952
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.009031337532820484
26-27	0.024869802637624575
28-29	0.009323194656332016
30-31	9.562647228907399E-5
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.027118525798876487
50-51	0.07140448869024782
52-53	0.0615515625307616
54-55	0.04063484502332782
56-57	0.005490960136773357
58-59	0.009713065106736245
60-61	0.03512129457934839
62-63	0.03540679424168686
64-65	0.0
66-67	0.0034707434304772754
68-69	0.0350500868177076
70-71	0.08949884217696626
72-73	0.08121144997721534
74-75	0.04065696565539948
76-77	0.0090831494510447
78-79	0.001141599182101266
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0018984885810887724
92-93	0.003899877912804023
94-95	0.001379830337964858
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	241.0
20-21	712.0
22-23	1644.0
24-25	4666.0
26-27	5272.0
28-29	7323.0
30-31	12196.0
32-33	13621.0
34-35	17559.0
36-37	18340.0
38-39	22005.0
40-41	29240.0
42-43	37874.0
44-45	50801.0
46-47	67176.0
48-49	40856.0
50-51	36235.0
52-53	35584.0
54-55	26602.0
56-57	29381.0
58-59	44875.0
60-61	30721.0
62-63	29329.0
64-65	49265.0
66-67	62443.0
68-69	34517.0
70-71	42118.0
72-73	35739.0
74-75	33141.0
76-77	24495.0
78-79	50170.0
80-81	64613.0
82-83	60939.0
84-85	88014.0
86-87	96125.0
88-89	102897.0
90-91	107642.0
92-93	114856.0
94-95	132525.0
96-97	308431.0
98-99	57446.0
100-101	5837880.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.20973978603132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.00999887458146	77.59738581118638
2	7.201923088937348	12.417518309125994
3	1.6512447001112496	4.270601277589623
4	0.5503066504087268	1.897671725370354
5	0.24474385377860713	1.0549651974242111
6	0.12994809676100125	0.6721674964473562
7	0.0669439965626939	0.403985716673477
8	0.03495568001736809	0.24108160626728617
9	0.026529586521845703	0.20583978756113894
>10	0.08152414520530321	1.0418866079456441
>50	0.0013745612578514243	0.07687535931427864
>100	4.404628271967881E-4	0.07294070942573609
>500	4.4202190734254143E-5	0.024864509627423433
>1k	2.2100838668856078E-5	0.022215886041105342
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2713735373006375E-5
2	0.0	2.542747074601275E-5	0.0	0.0	1.2713735373006375E-5
3	0.0	2.542747074601275E-5	0.0	1.2713735373006375E-5	1.2713735373006375E-5
4	0.0	6.356867686503188E-5	0.0	1.2713735373006375E-5	1.2713735373006375E-5
5	0.0	6.356867686503188E-5	0.0	1.2713735373006375E-5	2.542747074601275E-5
6	0.0	6.356867686503188E-5	0.0	1.2713735373006375E-5	2.542747074601275E-5
7	0.0	6.356867686503188E-5	0.0	1.2713735373006375E-5	2.542747074601275E-5
8	0.0	6.356867686503188E-5	0.0	2.542747074601275E-5	5.08549414920255E-5
9	0.0	1.9070603059509562E-4	0.0	3.814120611901912E-5	5.08549414920255E-5
10-11	0.0	2.03419765968102E-4	0.0	1.01709882984051E-4	5.08549414920255E-5
12-13	0.0	2.03419765968102E-4	0.0	1.01709882984051E-4	5.08549414920255E-5
14-15	0.0	2.2884723671411474E-4	0.0	1.3349422141656695E-4	7.628241223803825E-5
16-17	0.0	2.4156097208712112E-4	0.0	1.7163542753558607E-4	8.899614761104463E-5
18-19	0.0	2.8605904589264344E-4	0.0	1.8434916290859242E-4	9.535301529754782E-5
20-21	0.0	3.1784338432515937E-4	0.0	1.9070603059509562E-4	1.2713735373006375E-4
22-23	0.0	3.559845904441785E-4	0.0	2.2249036902761158E-4	1.3349422141656695E-4
24-25	0.0	3.6869832581718485E-4	0.0	3.242002520116626E-4	1.525648244760765E-4
26-27	0.0	3.8141206119019123E-4	0.0	5.021925472337518E-4	1.7163542753558607E-4
28-29	0.0	3.8141206119019123E-4	0.0	0.001048883168273026	1.7799229522208926E-4
30-31	0.0	4.06839531936204E-4	0.0	0.0018689190998319371	1.9070603059509564E-4
32-33	0.0	4.06839531936204E-4	0.0	0.0033691398738466896	2.03419765968102E-4
34-35	0.0	5.276200179797646E-4	0.0	0.005161776561440588	2.2884723671411474E-4
36-37	0.0	6.484005040233252E-4	0.0	0.006948056381347984	2.542747074601275E-4
38-39	0.0	6.484005040233252E-4	0.0	0.008416492816930219	2.542747074601275E-4
40-41	0.0	7.373966516343697E-4	0.0	0.010266341313702649	2.606315751466307E-4
42-43	0.0	7.81894725439892E-4	0.0	0.012109832942788572	2.797021782061402E-4
44-45	0.0	9.026752114834526E-4	0.0	0.014887784121790466	2.924159135791466E-4
46-47	0.0	9.662438883484845E-4	0.0	0.019070603059509562	3.05129648952153E-4
48-49	0.0	9.662438883484845E-4	0.0	0.024658289755945865	3.3055711969816575E-4
50-51	0.0	0.0011124518451380578	0.0	0.028688543869188884	3.3055711969816575E-4
52-53	0.0	0.001118808712824561	0.0	0.032343742788928216	3.496277227576753E-4
54-55	0.0	0.0011315224481975674	0.0	0.036558346065079836	3.6869832581718485E-4
56-57	0.0	0.00115694991894358	0.0	0.03882774782916147	3.6869832581718485E-4
58-59	0.0	0.0011633067866300834	0.0	0.04200618167241306	3.75055193503688E-4
60-61	0.0	0.0012141617281221087	0.0	0.044936697675891035	3.8141206119019123E-4
62-63	0.0	0.0012523029342411279	0.0	0.046379706640727256	3.8776892887669445E-4
64-65	0.0	0.0014430089648362235	0.0	0.047581154633476355	3.941257965631976E-4
66-67	0.0	0.00145572270020923	0.0	0.05023832532643469	3.941257965631976E-4
68-69	0.0	0.0015320051124472682	0.0	0.05327690808058321	4.004826642497008E-4
70-71	0.0	0.0015510757155067778	0.0	0.054986905488252566	4.3226700268221677E-4
72-73	0.0	0.001557432583193281	0.0	0.05637270264391027	5.212631502932613E-4
74-75	0.0	0.0016019306569988031	0.0	0.05721816604621519	5.212631502932613E-4
76-77	0.0	0.001665499333863835	0.0	0.05804455884546061	5.212631502932613E-4
78-79	0.0	0.0016909268046098479	0.0	0.05857217886344037	5.212631502932613E-4
80-81	0.0	0.0016909268046098479	0.0	0.05898537526306308	5.466906210392741E-4
82-83	0.0	0.001697283672296351	0.0	0.059506638413356336	5.721180917852869E-4
84-85	0.0	0.0017163542753558607	0.0	0.06001518782827659	5.721180917852869E-4
86-87	0.0	0.001729068010728867	0.0	0.06026310566805022	5.721180917852869E-4
88	0.0	0.001792636687593899	0.0	0.06028853313879623	5.721180917852869E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14205	0.0	54.421234	1
GTATCAA	26365	0.0	39.687294	1
TCAACGC	33255	0.0	31.007635	4
ATCAACG	33335	0.0	30.894571	3
TATCAAC	33855	0.0	30.625065	2
CAACGCA	34080	0.0	30.154793	5
AACGCAG	34290	0.0	30.007881	6
CGCAGAG	38670	0.0	26.59914	8
ACGCAGA	39430	0.0	26.12913	7
GTGGTAT	6410	0.0	25.379038	1
TGGTATC	6465	0.0	24.29081	2
GCAGAGT	41340	0.0	23.951286	9
GAGTACT	25925	0.0	23.631989	12-13
CAGAGTA	39465	0.0	22.020206	10-11
GTACATG	25560	0.0	21.673647	1
TACATGG	25450	0.0	21.51164	2
AGAGTAC	36835	0.0	21.414038	10-11
GTACTTT	28770	0.0	21.278399	14-15
AGTACTT	27675	0.0	20.396173	12-13
ACATGGG	26645	0.0	19.79793	3
>>END_MODULE