FastQCFastQC Report
Fri 27 May 2016
SRR2139285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8385725
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA146950.053.319531
GTATCAA273500.039.7577671
ATCAACG341350.031.3499073
TCAACGC342900.031.220584
TATCAAC346250.031.030912
CAACGCA350450.030.5725675
AACGCAG351750.030.5085896
CGCAGAG393650.027.1640228
TGGTATC62100.026.9972822
GTGGTAT65350.026.5120011
ACGCAGA404200.026.4964037
GCAGAGT434850.024.0554649
GAGTACT263800.023.5506312-13
CTTATAC49500.023.0729271
GTACATG289100.022.167921
TACATGG284700.022.1320632
CAGAGTA409750.022.03667410-11
AGAGTAC381500.021.72403310-11
GTACTTT292000.021.19083414-15
ACATGGG299150.020.6883033
Produced by FastQC (version 0.11.5)