##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139285_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8385725
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68449227705416	33.0	33.0	33.0	33.0	33.0
2	32.70081453899335	33.0	33.0	33.0	33.0	33.0
3	32.683596468999404	33.0	33.0	33.0	33.0	33.0
4	36.61547498874575	37.0	37.0	37.0	37.0	37.0
5	36.63774354632426	37.0	37.0	37.0	37.0	37.0
6	36.59724627268364	37.0	37.0	37.0	37.0	37.0
7	36.571880666251275	37.0	37.0	37.0	37.0	37.0
8	36.57751798443188	37.0	37.0	37.0	37.0	37.0
9	36.58880931583137	37.0	37.0	37.0	37.0	37.0
10-11	36.53464709372177	37.0	37.0	37.0	37.0	37.0
12-13	36.5015574085723	37.0	37.0	37.0	37.0	37.0
14-15	35.44872703314263	40.0	37.0	40.0	19.5	40.0
16-17	35.72181910329756	40.0	37.0	40.0	24.0	40.0
18-19	38.06433671507234	40.0	37.0	40.0	35.0	40.0
20-21	38.40109427722007	40.0	37.0	40.0	37.0	40.0
22-23	38.43751733932198	40.0	37.0	40.0	37.0	40.0
24-25	38.4716366380022	40.0	37.0	40.0	37.0	40.0
26-27	38.412169955243044	40.0	37.0	40.0	37.0	40.0
28-29	38.34102274264322	40.0	37.0	40.0	37.0	40.0
30-31	38.20870046465908	40.0	37.0	40.0	37.0	40.0
32-33	38.013803345111114	40.0	37.0	40.0	33.0	40.0
34-35	37.9278847806123	40.0	37.0	40.0	33.0	40.0
36-37	37.85846087476325	40.0	37.0	40.0	33.0	40.0
38-39	37.86574953249981	40.0	37.0	40.0	33.0	40.0
40-41	37.813413552128225	40.0	37.0	40.0	33.0	40.0
42-43	37.76366154420127	40.0	37.0	40.0	33.0	40.0
44-45	37.71344861183729	40.0	37.0	40.0	33.0	40.0
46-47	37.571599173495215	40.0	37.0	40.0	33.0	40.0
48-49	37.343508256267896	40.0	37.0	40.0	33.0	40.0
50-51	37.26026753997108	40.0	37.0	40.0	33.0	40.0
52-53	37.18722847418482	40.0	37.0	40.0	33.0	40.0
54-55	36.9710707014701	37.0	37.0	40.0	33.0	40.0
56-57	36.87346518333642	37.0	37.0	40.0	33.0	40.0
58-59	36.828170098741964	37.0	37.0	40.0	33.0	40.0
60-61	36.714878962076945	37.0	37.0	40.0	33.0	40.0
62-63	36.539534570441205	37.0	37.0	40.0	33.0	40.0
64-65	36.27546484228321	37.0	37.0	40.0	33.0	40.0
66-67	35.944291782117844	37.0	35.0	40.0	33.0	40.0
68-69	35.658356582634596	37.0	33.0	38.5	33.0	40.0
70-71	35.41937086556116	37.0	33.0	37.0	30.0	40.0
72-73	35.20615176230512	37.0	33.0	37.0	27.0	40.0
74-75	34.923828405159995	37.0	33.0	37.0	27.0	40.0
76-77	32.0300807305942	33.0	30.0	35.0	24.5	37.0
78-79	34.181777591460715	37.0	33.0	37.0	27.0	37.0
80-81	34.50626369156685	37.0	33.0	37.0	27.0	37.0
82-83	34.47860789398787	37.0	33.0	37.0	27.0	37.0
84-85	34.43576108978353	37.0	33.0	37.0	27.0	37.0
86-87	34.254509287316395	37.0	33.0	37.0	27.0	37.0
88-89	34.02727616126624	37.0	33.0	37.0	27.0	37.0
90-91	33.87200403184899	37.0	33.0	37.0	27.0	37.0
92-93	33.88864093555095	37.0	33.0	37.0	27.0	37.0
94-95	33.818527191184145	35.0	33.0	37.0	27.0	37.0
96-97	33.76299023094531	33.0	33.0	37.0	27.0	37.0
98-99	33.74228537090795	33.0	33.0	37.0	27.0	37.0
100	33.751555437450016	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	3.0
18	4.0
19	13.0
20	70.0
21	307.0
22	1218.0
23	3583.0
24	8733.0
25	17475.0
26	30876.0
27	50907.0
28	76314.0
29	108645.0
30	149277.0
31	201292.0
32	271100.0
33	370248.0
34	522949.0
35	815503.0
36	1452440.0
37	2605734.0
38	1668475.0
39	30558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.274885597100024	20.083942762655628	14.68813508307104	22.953036557173306
2	14.74115748303238	21.20017009954353	41.49899387980316	22.559678537620933
3	18.154227613365094	26.458123598938464	30.694919365398277	24.69272942229816
4	12.456513689698575	18.37815276867662	38.91184798856623	30.253485553058574
5	13.288426822843316	37.84780265685061	35.2059775919009	13.657792928405177
6	29.375134529214826	38.06280315655474	18.819780042870473	13.74228227135996
7	26.129547534649657	33.39286704488879	22.690488896308906	17.787096524152652
8	24.731469252807596	36.14435245610845	21.061351284474508	18.062827006609446
9	25.72451398060394	17.01935133813713	21.167758303545607	36.08837637771332
10-11	24.063906221584897	27.120714070637895	28.497136502806853	20.31824320497035
12-13	25.193832375853013	24.750817609687893	28.819833705493565	21.235516308965533
14-15	22.586238649063134	25.689219216623442	26.95416431224153	24.770377822071893
16-17	21.456761341446327	29.173583679407567	27.438402761836333	21.931252217309773
18-19	21.40922818241714	27.925069090627225	29.802080321021734	20.863622405933892
20-21	22.353177303472567	26.948250501650623	29.87468096022119	20.82389123465562
22-23	22.020446741112785	26.943231763963176	29.74092469525551	21.295396799668524
24-25	21.743663469688205	27.134138684032006	29.847560308085452	21.27463753819434
26-27	21.706779267910825	27.31096969622798	29.72779707896246	21.254453956898743
28-29	21.517697366177188	27.365742939471765	29.76901374597192	21.34754594837912
30-31	21.94734787917864	27.162953915941994	29.61455199947578	21.275146205403587
32-33	21.730423293191063	27.374156863228823	29.5660220566723	21.329397786907816
34-35	21.753600858744175	27.463534652883943	29.56083831925156	21.222026169120323
36-37	21.79486894340264	27.411793227283248	29.45113067464538	21.34220715466873
38-39	21.66215062879615	27.476407262767594	29.47536420084691	21.386077907589343
40-41	21.946838178862542	27.35336987679058	29.274293486876417	21.425498457470464
42-43	21.937844821701475	27.471322907979445	29.011689472381345	21.57914279793773
44-45	21.95988675568319	27.72091599279834	28.66884805548937	21.650349196029097
46-47	22.154655709287653	27.62542748957554	28.24608539975334	21.973831401383464
48-49	22.04350168114261	27.826065320419787	28.19081474652016	21.93961825191744
50-51	22.0010775976044	28.013742480303666	28.072705485481986	21.912474436609948
52-53	22.232480761282993	27.943664878765723	27.908826796825455	21.91502756312583
54-55	22.17900260609957	27.89449938849753	27.8733527448356	22.0531452605673
56-57	22.20590790880195	28.02402203366884	27.762992317313927	22.007077740215287
58-59	22.140318332674937	28.144313710140505	27.82060667491989	21.894761282264668
60-61	22.230308104040446	28.12818693042528	27.766545493434812	21.87495947209946
62-63	22.18760564735038	28.23173034623333	27.659914966460963	21.92074903995532
64-65	22.188997183251917	28.175432084423964	27.64719727650785	21.98837345581627
66-67	22.27663436808675	28.064750373915576	27.54754204484875	22.111073213148924
68-69	22.197019337356817	28.063456090651556	27.66953565710063	22.069988914891
70-71	22.216918053231264	27.85149119478223	27.679082583533233	22.25250816845327
72-73	22.24384967617604	27.906861661810783	27.63193527386594	22.217353388147238
74-75	22.061333255320793	28.030363838134058	27.589340817895895	22.31896208864925
76-77	22.183972900922218	27.906195209354028	27.57641653733541	22.333415352388336
78-79	22.216583454074307	27.96378579284511	27.53467553678865	22.284955216291937
80-81	22.21756951359013	27.975947094456515	27.50941831051337	22.29706508143998
82-83	22.249640242342064	27.884590352623434	27.621834326474954	22.243935078559552
84-85	22.384345752633116	27.86601678942982	27.51511672487666	22.2345207330604
86-87	22.166675201859682	27.892906601146688	27.615151769345513	22.325266427648117
88-89	22.257152230938033	27.818412025774208	27.59603916904034	22.32839657424742
90-91	22.299329404964503	27.811958108950552	27.620191137339283	22.268521348745658
92-93	22.30192287863385	27.72634128201587	27.636303942651264	22.335431896699014
94-95	22.237121667820915	27.80795846832216	27.638530583265506	22.31638928059142
96-97	22.188534302537583	27.960537852678186	27.73249894297542	22.118428901808812
98-99	23.300449764584698	29.865686322234435	27.877093181174033	18.95677073200683
100	22.196937656495034	30.81280195109397	21.786247632022594	25.2040127603884
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2009.0
1	1812.0
2	2615.5
3	3940.0
4	5736.5
5	9608.5
6	12663.0
7	14671.5
8	16348.0
9	16521.5
10	15614.5
11	14778.0
12	16385.0
13	22095.0
14	35080.0
15	52343.5
16	67454.0
17	77130.5
18	77082.0
19	69350.0
20	59682.0
21	49931.0
22	42993.0
23	41807.0
24	45747.5
25	54820.0
26	68310.5
27	84339.5
28	100652.5
29	118102.5
30	135385.5
31	152269.0
32	170988.0
33	189488.5
34	207890.0
35	224610.0
36	242069.0
37	257680.0
38	266755.0
39	273760.5
40	283530.5
41	292452.5
42	296543.0
43	301394.0
44	307385.5
45	312339.5
46	316321.5
47	318312.0
48	318858.5
49	316220.0
50	311013.5
51	302514.0
52	290572.0
53	277397.5
54	263008.0
55	245446.0
56	224572.0
57	203081.0
58	180868.0
59	158306.0
60	134129.5
61	109689.0
62	86753.0
63	66870.0
64	50404.5
65	37883.5
66	29307.0
67	22715.5
68	17368.0
69	12362.5
70	8651.0
71	6447.0
72	4732.5
73	3421.5
74	2471.0
75	1748.0
76	1184.0
77	802.0
78	571.5
79	426.5
80	317.0
81	236.5
82	183.0
83	125.0
84	86.0
85	68.0
86	55.5
87	47.0
88	32.5
89	15.0
90	5.5
91	4.0
92	4.0
93	4.0
94	3.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.311753008833464E-4
2	5.604762855924801E-4
3	0.0014190782550107474
4	4.770010941212597E-4
5	8.347519147122044E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.016101768183431
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008596101009018807
26-27	0.02342060293250539
28-29	0.00891115962078835
30-31	1.3153327259684823E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.02674975777948918
50-51	0.07111314263886337
52-53	0.06036953145377448
54-55	0.039780759356003184
56-57	0.005221963439310145
58-59	0.009219056342221537
60-61	0.03406536833802195
62-63	0.03512574921809054
64-65	0.0
66-67	0.0031545636466130053
68-69	0.03460640468020432
70-71	0.08744555689125436
72-73	0.07957826044046534
74-75	0.03958862890913453
76-77	0.008763373927296243
78-79	9.511084727664015E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0017277983946263713
92-93	0.0033723779761235637
94-95	0.001236407763639493
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	244.0
20-21	787.0
22-23	1733.0
24-25	4425.0
26-27	5375.0
28-29	7441.0
30-31	12287.0
32-33	13411.0
34-35	17605.0
36-37	20127.0
38-39	27934.0
40-41	31954.0
42-43	41024.0
44-45	58140.0
46-47	51427.0
48-49	40591.0
50-51	34742.0
52-53	31535.0
54-55	52679.0
56-57	49999.0
58-59	44179.0
60-61	49261.0
62-63	43274.0
64-65	50622.0
66-67	69260.0
68-69	37465.0
70-71	43899.0
72-73	57967.0
74-75	61493.0
76-77	48005.0
78-79	59062.0
80-81	75707.0
82-83	86130.0
84-85	92441.0
86-87	98448.0
88-89	107189.0
90-91	112520.0
92-93	120355.0
94-95	141556.0
96-97	325343.0
98-99	547288.0
100-101	5610801.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.16297406928591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.28232916495152	77.78993986754453
2	6.924658802893516	11.932983937447329
3	1.6175190994141462	4.181107686581874
4	0.5581101947858338	1.9235373696454365
5	0.24610797794592482	1.0602697660999554
6	0.11987927227293252	0.619749277697853
7	0.07846642262018527	0.4732630236272858
8	0.04132486138736053	0.2848538368108784
9	0.03169317161911728	0.24577001319522873
>10	0.09760046410479635	1.2523034094094938
>50	0.0016558264619141627	0.09925189049096216
>100	5.698070700094815E-4	0.08248608119697091
>500	6.370117541107695E-5	0.03435907576452614
>1k	2.123329729618367E-5	0.020124764487701636
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1925027353031492E-5	0.0	1.1925027353031492E-5	0.0
3	0.0	1.1925027353031492E-5	0.0	1.1925027353031492E-5	0.0
4	0.0	3.577508205909447E-5	0.0	1.1925027353031492E-5	0.0
5	0.0	3.577508205909447E-5	0.0	2.3850054706062983E-5	0.0
6	0.0	3.577508205909447E-5	0.0	2.3850054706062983E-5	0.0
7	0.0	3.577508205909447E-5	0.0	3.577508205909447E-5	0.0
8	0.0	3.577508205909447E-5	0.0	3.577508205909447E-5	1.1925027353031492E-5
9	0.0	1.0732524617728341E-4	0.0	5.962513676515745E-5	1.1925027353031492E-5
10-11	0.0	1.0732524617728341E-4	0.0	6.55876504416732E-5	1.1925027353031492E-5
12-13	0.0	1.0732524617728341E-4	0.0	7.155016411818895E-5	1.1925027353031492E-5
14-15	0.0	1.7887541029547237E-4	0.0	7.155016411818895E-5	4.173759573561022E-5
16-17	0.0	1.7887541029547237E-4	0.0	7.155016411818895E-5	6.55876504416732E-5
18-19	0.0	1.8483792397198812E-4	0.0	9.540021882425193E-5	7.155016411818895E-5
20-21	0.0	1.9080043764850387E-4	0.0	1.192502735303149E-4	7.155016411818895E-5
22-23	0.0	2.2061300603108257E-4	0.0	1.3713781455986215E-4	7.751267779470469E-5
24-25	5.962513676515746E-6	2.504255744136613E-4	0.0	2.0272546500153533E-4	8.347519147122044E-5
26-27	1.1925027353031492E-5	2.623506017666928E-4	0.0	3.3390076588488176E-4	8.347519147122044E-5
28-29	1.1925027353031492E-5	2.6831311544320854E-4	0.0	9.063020788303934E-4	8.347519147122044E-5
30-31	1.1925027353031492E-5	3.1005071117881877E-4	0.0	0.0017649040482486606	8.347519147122044E-5
32-33	1.1925027353031492E-5	3.1005071117881877E-4	0.0	0.0032495699537010815	8.347519147122044E-5
34-35	1.7887541029547237E-5	3.816008752970077E-4	0.0	0.005103911707097479	9.540021882425193E-5
36-37	2.3850054706062983E-5	4.5315103941519664E-4	0.0	0.007363704390496946	9.540021882425193E-5
38-39	2.3850054706062983E-5	4.710385804447439E-4	0.0	0.011722301888029956	9.540021882425193E-5
40-41	2.3850054706062983E-5	5.902888539750589E-4	0.0	0.016879876218216076	9.540021882425193E-5
42-43	2.3850054706062983E-5	6.08176395004606E-4	0.0	0.019646482564119383	9.540021882425193E-5
44-45	2.9812568382578726E-5	7.03576613828858E-4	0.0	0.02271717710752499	9.540021882425193E-5
46-47	3.577508205909447E-5	7.870518053000784E-4	0.0	0.026396048045935205	9.540021882425193E-5
48-49	3.577508205909447E-5	7.870518053000784E-4	0.0	0.029049366631984715	9.540021882425193E-5
50-51	3.577508205909447E-5	8.943770514773618E-4	0.0	0.03167883516332815	9.540021882425193E-5
52-53	3.577508205909447E-5	9.063020788303933E-4	0.0	0.034630279433203445	1.0732524617728341E-4
54-55	4.173759573561022E-5	9.063020788303933E-4	0.0	0.03811238742028865	1.192502735303149E-4
56-57	4.7700109412125966E-5	9.063020788303933E-4	0.0	0.0467282196828539	1.192502735303149E-4
58-59	4.7700109412125966E-5	9.301521335364563E-4	0.0	0.052058706909658976	1.2521278720683065E-4
60-61	5.962513676515745E-5	9.718897292720664E-4	0.0	0.055290389322330505	1.311753008833464E-4
62-63	5.962513676515745E-5	0.0010017022976546452	0.0	0.05774694495705499	1.311753008833464E-4
64-65	5.962513676515745E-5	0.0010732524617728342	0.0	0.06037045097472192	1.311753008833464E-4
66-67	5.962513676515745E-5	0.0011149900575084444	0.0	0.06398373426269047	1.311753008833464E-4
68-69	7.155016411818895E-5	0.0011328775985379918	0.0	0.0675612424685999	1.3713781455986215E-4
70-71	7.155016411818895E-5	0.0011448026258910231	0.0	0.06955868455023269	1.431003282363779E-4
72-73	7.155016411818895E-5	0.0011448026258910231	0.0	0.07137725122156999	1.431003282363779E-4
74-75	7.155016411818895E-5	0.001174615194273602	0.0	0.0737324441237937	1.431003282363779E-4
76-77	7.155016411818895E-5	0.0012103902763326963	0.0	0.07633210008675456	1.431003282363779E-4
78-79	7.155016411818895E-5	0.001240202844715275	0.0	0.07776310336911835	1.431003282363779E-4
80-81	7.155016411818895E-5	0.001240202844715275	0.0	0.0781208541897093	1.431003282363779E-4
82-83	7.155016411818895E-5	0.001240202844715275	0.0	0.07878269320780254	1.431003282363779E-4
84-85	7.751267779470469E-5	0.0012461653583917907	0.0	0.07938490708913062	1.431003282363779E-4
86-87	8.347519147122044E-5	0.0012521278720683065	0.0	0.07980824556016325	1.431003282363779E-4
88	8.347519147122044E-5	0.0012759779267743695	0.0	0.07983805812854583	1.431003282363779E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14695	0.0	53.31953	1
GTATCAA	27350	0.0	39.757767	1
ATCAACG	34135	0.0	31.349907	3
TCAACGC	34290	0.0	31.22058	4
TATCAAC	34625	0.0	31.03091	2
CAACGCA	35045	0.0	30.572567	5
AACGCAG	35175	0.0	30.508589	6
CGCAGAG	39365	0.0	27.164022	8
TGGTATC	6210	0.0	26.997282	2
GTGGTAT	6535	0.0	26.512001	1
ACGCAGA	40420	0.0	26.496403	7
GCAGAGT	43485	0.0	24.055464	9
GAGTACT	26380	0.0	23.55063	12-13
CTTATAC	4950	0.0	23.072927	1
GTACATG	28910	0.0	22.16792	1
TACATGG	28470	0.0	22.132063	2
CAGAGTA	40975	0.0	22.036674	10-11
AGAGTAC	38150	0.0	21.724033	10-11
GTACTTT	29200	0.0	21.190834	14-15
ACATGGG	29915	0.0	20.688303	3
>>END_MODULE