FastQCFastQC Report
Fri 27 May 2016
SRR2139284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8142902
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145700.053.1196021
CTCGTAT35750.041.639579
GTATCAA268900.039.9499171
AATCTCG36800.039.4922336
TCTCGTA39900.035.656388
TCAACGC334300.031.649324
ATCAACG335500.031.5100233
TATCAAC342900.031.0476112
CAACGCA343200.030.7773695
AACGCAG349700.030.2303336
ATCTCGT47200.029.9558727
CGCAGAG387500.027.2817488
ACGCAGA396250.026.6789867
GTGGTAT66850.025.6694621
GAGTACT250150.024.62154412-13
GCAGAGT417950.024.3811559
TGGTATC69550.023.162362
GTACATG259450.022.5416071
CAGAGTA390000.022.43612110-11
AGAGTAC360200.022.34664510-11
Produced by FastQC (version 0.11.5)