##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8142902
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70292433827645	33.0	33.0	33.0	33.0	33.0
2	32.70892539293731	33.0	33.0	33.0	33.0	33.0
3	32.7040746652729	33.0	33.0	33.0	33.0	33.0
4	36.6373328574997	37.0	37.0	37.0	37.0	37.0
5	36.655225372969	37.0	37.0	37.0	37.0	37.0
6	36.61690218057395	37.0	37.0	37.0	37.0	37.0
7	36.59251333247041	37.0	37.0	37.0	37.0	37.0
8	36.58369141615606	37.0	37.0	37.0	37.0	37.0
9	36.59343290635206	37.0	37.0	37.0	37.0	37.0
10-11	36.553406144394216	37.0	37.0	37.0	37.0	37.0
12-13	36.5185422838197	37.0	37.0	37.0	37.0	37.0
14-15	35.440159110351566	40.0	37.0	40.0	19.5	40.0
16-17	35.745797628413065	40.0	37.0	40.0	24.0	40.0
18-19	38.124110789986176	40.0	37.0	40.0	35.0	40.0
20-21	38.46118802539998	40.0	37.0	40.0	37.0	40.0
22-23	38.50509013454885	40.0	37.0	40.0	37.0	40.0
24-25	38.536395737083794	40.0	37.0	40.0	37.0	40.0
26-27	38.47513699028897	40.0	37.0	40.0	37.0	40.0
28-29	38.41188170928519	40.0	37.0	40.0	37.0	40.0
30-31	38.275686091430536	40.0	37.0	40.0	37.0	40.0
32-33	38.09799258728227	40.0	37.0	40.0	35.0	40.0
34-35	38.022752704670374	40.0	37.0	40.0	33.0	40.0
36-37	37.95717418839576	40.0	37.0	40.0	33.0	40.0
38-39	37.959699564175324	40.0	37.0	40.0	33.0	40.0
40-41	37.90543403888302	40.0	37.0	40.0	33.0	40.0
42-43	37.854791018988955	40.0	37.0	40.0	33.0	40.0
44-45	37.803352250317545	40.0	37.0	40.0	33.0	40.0
46-47	37.66046618983507	40.0	37.0	40.0	33.0	40.0
48-49	37.43582230143424	40.0	37.0	40.0	33.0	40.0
50-51	37.34865932632634	40.0	37.0	40.0	33.0	40.0
52-53	37.269786458307095	40.0	37.0	40.0	33.0	40.0
54-55	37.05662484575376	37.0	37.0	40.0	33.0	40.0
56-57	36.95172543462647	37.0	37.0	40.0	33.0	40.0
58-59	36.89387542623862	37.0	37.0	40.0	33.0	40.0
60-61	36.77315694550653	37.0	37.0	40.0	33.0	40.0
62-63	36.58862159492759	37.0	37.0	40.0	33.0	40.0
64-65	36.3216925669528	37.0	37.0	40.0	33.0	40.0
66-67	35.995017887150816	37.0	37.0	40.0	33.0	40.0
68-69	35.71224458529235	37.0	33.0	37.0	33.0	40.0
70-71	35.47670743673549	37.0	33.0	37.0	33.0	40.0
72-73	35.269616834835816	37.0	33.0	37.0	33.0	40.0
74-75	34.99908664262825	37.0	33.0	37.0	30.0	40.0
76-77	32.13223834191693	33.0	30.0	35.0	24.5	37.0
78-79	34.252738918024235	37.0	33.0	37.0	27.0	37.0
80-81	34.58585804031992	37.0	33.0	37.0	27.0	37.0
82-83	34.565754693994734	37.0	33.0	37.0	33.0	37.0
84-85	34.53099281617909	37.0	33.0	37.0	33.0	37.0
86-87	34.35964621316198	37.0	33.0	37.0	27.0	37.0
88-89	34.13777522275997	37.0	33.0	37.0	27.0	37.0
90-91	33.98864902341107	37.0	33.0	37.0	27.0	37.0
92-93	34.00619724388221	37.0	33.0	37.0	27.0	37.0
94-95	33.94460544849432	37.0	33.0	37.0	27.0	37.0
96-97	33.889237587064585	35.0	33.0	37.0	27.0	37.0
98-99	33.871642047471674	33.0	33.0	37.0	27.0	37.0
100	33.91249722878863	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	8.0
19	9.0
20	68.0
21	331.0
22	1149.0
23	3451.0
24	8329.0
25	16715.0
26	30013.0
27	48861.0
28	73535.0
29	104317.0
30	143212.0
31	190695.0
32	254446.0
33	346366.0
34	485639.0
35	758543.0
36	1400235.0
37	2640201.0
38	1618898.0
39	17879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.769752110488376	19.687231898406736	14.468208017240045	23.074807973864846
2	14.90891589796655	20.898875935344087	41.02959415114519	23.16261401554417
3	18.284517081389893	26.245360920963112	30.364789420812055	25.10533257683494
4	12.939563944952583	18.020413193331933	38.26760856213981	30.77241429957567
5	13.606886196181483	37.6750466879179	34.79382465630484	13.924242459595778
6	29.963101606773606	37.63223479786444	18.46453512519247	13.94012847016948
7	26.545695871078884	33.19793115525644	22.32995067360506	17.92642230005961
8	25.01328150578258	35.69238583492715	20.868346444547655	18.425986214742608
9	25.864255765327886	16.880394729053595	20.87765516519786	36.37769434042065
10-11	24.40434012345967	26.693174006023895	28.20391919244515	20.69856667807128
12-13	25.62329130327247	24.32132917723927	28.525941979898565	21.529437539589694
14-15	22.86595543098357	25.34800269062783	26.62764585683287	25.158396021555728
16-17	21.938499321249353	28.682077961886314	27.036276501915413	22.343146214948924
18-19	21.88581540094674	27.455770682245717	29.372169774362998	21.28624414244455
20-21	22.689019074887952	26.49872803786731	29.553310036747753	21.258942850496982
22-23	22.422164109839894	26.44057987071063	29.5727282346893	21.564527784760177
24-25	22.12586215963955	26.661873682461806	29.468693891734425	21.74357026616422
26-27	22.174441581609017	26.752575091741516	29.332342362874343	21.740640963775128
28-29	21.976754893467874	26.819299859069307	29.553186773859537	21.650758473603283
30-31	22.40066198049106	26.762928205853814	29.202882193801084	21.633527619854046
32-33	22.075563132898445	27.08969830033298	29.117957782629507	21.716780784139065
34-35	22.22123602247152	27.071664932890442	29.078540248129503	21.628558796508532
36-37	22.2696002180523	27.017828945600485	28.95937116530799	21.75319967103923
38-39	22.128295480470616	27.05040471965158	29.05822314417389	21.763076655703912
40-41	22.432959688990604	26.905794560014673	28.843727883474653	21.81751786752007
42-43	22.315819120117876	27.083776914970457	28.574586403699904	22.025817561211763
44-45	22.43550802853916	27.276566184233552	28.2717266086447	22.01619917858259
46-47	22.58617094809841	27.29059862540958	27.795366980766094	22.327863445725917
48-49	22.500838323520654	27.462665462894016	27.686279805743975	22.35021640784136
50-51	22.50539152614216	27.61353647761109	27.580467260071508	22.30060473617525
52-53	22.713507182469172	27.5236522930278	27.38232645954061	22.380514064962416
54-55	22.62671878672407	27.47353141392062	27.453651316559448	22.446098482795858
56-57	22.647617978203005	27.66822771090513	27.338127275558165	22.346027035333698
58-59	22.57645755707144	27.70165625362977	27.35270743481403	22.369178754484764
60-61	22.678400486843895	27.63123261880039	27.385429652387362	22.304937241968357
62-63	22.589667514627354	27.778208056828426	27.307388764874513	22.324735663669713
64-65	22.658284144578904	27.73450610587817	27.227760065550306	22.37944968399262
66-67	22.700453020971324	27.674336561472295	27.11069457233957	22.514515845216813
68-69	22.637360906901097	27.64590343208239	27.261943027159685	22.454792633856822
70-71	22.669544062310894	27.420600214440714	27.24130967854186	22.66854604470653
72-73	22.688471738686946	27.48916602288195	27.17590873975379	22.646453498677317
74-75	22.491270232830786	27.66061073225788	27.203188757021735	22.644930277889603
76-77	22.748012079485626	27.53661804248077	27.08654423548123	22.628825642552375
78-79	22.636154936630298	27.536490271702245	27.079731189416844	22.747623602250613
80-81	22.646017496661457	27.52981299038848	27.13527935689645	22.688890156053613
82-83	22.67644612877472	27.504091285851445	27.20822416996387	22.611238415409964
84-85	22.776471808177412	27.488172453235666	27.152272481834995	22.58308325675193
86-87	22.61648940186356	27.508063296067164	27.200758459268393	22.674688842800883
88-89	22.62708181775955	27.4532591531765	27.1815602089228	22.738098820141143
90-91	22.704444560324504	27.414949615527785	27.226588348440036	22.65401747570768
92-93	22.729921621744396	27.3523328727884	27.217354337039488	22.700391168427718
94-95	22.634255330765633	27.399254398259306	27.239768553197564	22.726721717777497
96-97	22.664638932616132	27.48966657102076	27.319744680486586	22.52594981587653
98-99	22.864396939199054	28.31007969588561	26.332625434139846	22.492897930775495
100	20.94536331225494	27.917940564605313	27.561759345426985	23.574936777712757
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1770.0
1	1634.0
2	2388.0
3	3537.0
4	5110.5
5	8713.0
6	11638.5
7	13626.0
8	15306.5
9	15564.0
10	14567.0
11	13516.0
12	14883.0
13	20033.0
14	32171.5
15	48752.0
16	63193.5
17	72330.0
18	72377.5
19	64729.5
20	54671.5
21	44785.0
22	37514.5
23	35333.5
24	38005.0
25	45196.0
26	56105.5
27	68463.0
28	82337.5
29	98282.0
30	114104.5
31	129152.0
32	145465.0
33	162701.0
34	178563.0
35	192992.0
36	209904.0
37	225913.0
38	236900.0
39	247100.0
40	261055.0
41	273742.0
42	279108.5
43	283178.0
44	288093.0
45	293765.5
46	299742.0
47	303663.5
48	306797.5
49	307512.5
50	304775.0
51	300491.0
52	293957.0
53	284371.0
54	272866.0
55	257821.5
56	238849.5
57	216419.0
58	193536.0
59	170552.0
60	144039.0
61	118380.0
62	95018.5
63	73963.0
64	55925.0
65	41570.5
66	31606.0
67	24354.5
68	18638.0
69	13531.5
70	9628.0
71	7120.0
72	5178.5
73	3651.0
74	2611.5
75	1823.5
76	1237.0
77	824.0
78	556.5
79	396.5
80	291.5
81	209.0
82	157.0
83	113.5
84	82.5
85	63.5
86	50.0
87	38.0
88	28.0
89	18.0
90	9.5
91	5.0
92	3.5
93	2.5
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	5.280672664364621E-4
3	0.0014982373605871716
4	3.806996571983796E-4
5	3.684190230952061E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.218409358236166
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008889290248227441
26-27	0.024671854804106146
28-29	0.0091152830513736
30-31	1.1083075036112353E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.027376573629075685
50-51	0.07071871655981742
52-53	0.06004228086501547
54-55	0.04040439523921735
56-57	0.0057877415248653915
58-59	0.009321056926465775
60-61	0.03490510733320505
62-63	0.035646935934733386
64-65	0.0
66-67	0.0036480138569313383
68-69	0.034982271085549015
70-71	0.08696474750142542
72-73	0.0787973084603848
74-75	0.03983686049232308
76-77	0.009540216909288404
78-79	0.001349514212181746
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0017766889739837845
92-93	0.003302252774827969
94-95	0.0011352771618608718
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	214.0
20-21	813.0
22-23	1765.0
24-25	4144.0
26-27	5073.0
28-29	7683.0
30-31	11533.0
32-33	12606.0
34-35	17589.0
36-37	25938.0
38-39	23651.0
40-41	27937.0
42-43	34261.0
44-45	44429.0
46-47	43417.0
48-49	30315.0
50-51	26275.0
52-53	19642.0
54-55	23456.0
56-57	30286.0
58-59	37062.0
60-61	20768.0
62-63	34738.0
64-65	31026.0
66-67	59348.0
68-69	32876.0
70-71	44601.0
72-73	45250.0
74-75	35280.0
76-77	23205.0
78-79	53843.0
80-81	45987.0
82-83	53955.0
84-85	85591.0
86-87	94010.0
88-89	103434.0
90-91	109432.0
92-93	115300.0
94-95	135783.0
96-97	325191.0
98-99	58523.0
100-101	6206672.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.44337386711729
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.04607770647468	75.19351231168218
2	7.674147503710152	12.960618135344024
3	1.8452016473000277	4.674451576895308
4	0.6783626098023848	2.2913290990806474
5	0.31480560647856215	1.329162376166691
6	0.14885377230529673	0.7541828847784151
7	0.085910389191392	0.5078194179490787
8	0.05497193568085584	0.37136125735181075
9	0.03718884979158949	0.2826316751975333
>10	0.11191067607051224	1.4194187185445202
>50	0.002068788698631831	0.11523550543100992
>100	4.531610570494268E-4	0.0641089486368402
>500	2.36767327041237E-5	0.01345557968004759
>1k	2.367670623031216E-5	0.022712513261843037
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2280634103173536E-5	0.0	0.0	0.0
3	0.0	3.6841902309520614E-5	0.0	0.0	0.0
4	0.0	4.9122536412694145E-5	0.0	0.0	1.2280634103173536E-5
5	1.2280634103173536E-5	6.140317051586768E-5	0.0	1.2280634103173536E-5	1.2280634103173536E-5
6	3.6841902309520614E-5	6.140317051586768E-5	0.0	1.2280634103173536E-5	2.4561268206347072E-5
7	3.6841902309520614E-5	7.368380461904123E-5	0.0	2.4561268206347072E-5	2.4561268206347072E-5
8	4.9122536412694145E-5	1.1052570692856183E-4	0.0	2.4561268206347072E-5	2.4561268206347072E-5
9	4.9122536412694145E-5	1.350869751349089E-4	0.0	2.4561268206347072E-5	2.4561268206347072E-5
10-11	6.140317051586768E-5	1.350869751349089E-4	0.0	9.210475577380153E-5	2.4561268206347072E-5
12-13	6.140317051586768E-5	1.350869751349089E-4	0.0	1.4736760923808246E-4	3.6841902309520614E-5
14-15	6.754348756745446E-5	1.5964824334125597E-4	0.0	1.6578856039284275E-4	3.6841902309520614E-5
16-17	8.596443872221476E-5	1.5964824334125597E-4	0.0	2.149110968055369E-4	4.298221936110738E-5
18-19	9.824507282538829E-5	1.8420951154760306E-4	0.0	2.763142673214046E-4	4.9122536412694145E-5
20-21	1.2280634103173537E-4	2.0877077975395012E-4	0.0	3.1929648668251195E-4	4.9122536412694145E-5
22-23	1.2894665808332214E-4	2.578933161666443E-4	0.0	3.9912060835314E-4	6.754348756745446E-5
24-25	1.5964824334125597E-4	3.131561696309252E-4	0.0	4.543834618174209E-4	9.210475577380153E-5
26-27	1.7192887744442952E-4	3.315771207856855E-4	0.0	5.771898028491562E-4	9.824507282538829E-5
28-29	1.7192887744442952E-4	3.315771207856855E-4	0.0	0.001160519922749899	9.824507282538829E-5
30-31	2.149110968055369E-4	3.9298029130155316E-4	0.0	0.0021613916021585426	1.0438538987697506E-4
32-33	2.2105141385712367E-4	3.9912060835313993E-4	0.0	0.0034017356465790697	1.2280634103173537E-4
34-35	2.3947236501188396E-4	4.605237788690076E-4	0.0	0.004783306983186093	1.350869751349089E-4
36-37	2.578933161666443E-4	5.157866323332886E-4	0.0	0.0065148763917335616	1.350869751349089E-4
38-39	2.578933161666443E-4	5.403479005396356E-4	0.0	0.00909994987045159	1.4122729218649568E-4
40-41	2.578933161666443E-4	6.140317051586768E-4	0.0	0.011218359253249027	1.4736760923808246E-4
42-43	2.578933161666443E-4	6.201720222102636E-4	0.0	0.012544667736391768	1.4736760923808246E-4
44-45	2.701739502698178E-4	6.877155097777181E-4	0.0	0.01339817180656233	1.535079262896692E-4
46-47	2.8245458437299137E-4	7.368380461904122E-4	0.0	0.014245535559681301	1.5964824334125597E-4
48-49	2.9473521847616486E-4	7.368380461904122E-4	0.0	0.01520342501972884	1.7192887744442952E-4
50-51	3.070158525793384E-4	8.22802484912627E-4	0.0	0.01627184018670494	1.7192887744442952E-4
52-53	3.070158525793384E-4	8.22802484912627E-4	0.0	0.0182367416432127	1.9034982859918983E-4
54-55	3.070158525793384E-4	8.412234360673873E-4	0.0	0.01959375171161338	2.0877077975395012E-4
56-57	3.070158525793384E-4	8.596443872221476E-4	0.0	0.021527951582863213	2.0877077975395012E-4
58-59	3.070158525793384E-4	8.596443872221476E-4	0.0	0.023726185087331274	2.0877077975395012E-4
60-61	3.1929648668251195E-4	8.842056554284947E-4	0.0	0.025261264350227963	2.0877077975395012E-4
62-63	3.1929648668251195E-4	9.02626606583255E-4	0.0	0.02614547000565646	2.0877077975395012E-4
64-65	3.1929648668251195E-4	0.001031573264666577	0.0	0.02718318358737462	2.0877077975395012E-4
66-67	3.315771207856855E-4	0.001031573264666577	0.0	0.029381417091842686	2.0877077975395012E-4
68-69	3.315771207856855E-4	0.0010499942158213374	0.0	0.03178228105901311	2.0877077975395012E-4
70-71	3.315771207856855E-4	0.0010745554840276845	0.0	0.03340332476063202	2.0877077975395012E-4
72-73	3.315771207856855E-4	0.001086836118130858	0.0	0.03499366687699299	2.578933161666443E-4
74-75	3.3771743783727226E-4	0.0011236780204403787	0.0	0.0359208547517826	2.578933161666443E-4
76-77	3.4385775488885903E-4	0.0011789408739046596	0.0	0.03668225406617935	2.578933161666443E-4
78-79	3.561383889920326E-4	0.0011912215080078332	0.0	0.03713049721094519	2.578933161666443E-4
80-81	3.561383889920326E-4	0.0011973618250594197	0.0	0.03736996957595708	2.763142673214046E-4
82-83	3.6227870604361935E-4	0.0012035021421110065	0.0	0.03776294986725863	3.3771743783727226E-4
84-85	3.684190230952061E-4	0.0012035021421110065	0.0	0.038119088256250655	3.499980719404458E-4
86-87	3.684190230952061E-4	0.0012035021421110065	0.0	0.038303297767798256	3.561383889920326E-4
88	3.684190230952061E-4	0.001326308483142742	0.0	0.03831557840190143	3.684190230952061E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14570	0.0	53.119602	1
CTCGTAT	3575	0.0	41.63957	9
GTATCAA	26890	0.0	39.949917	1
AATCTCG	3680	0.0	39.492233	6
TCTCGTA	3990	0.0	35.65638	8
TCAACGC	33430	0.0	31.64932	4
ATCAACG	33550	0.0	31.510023	3
TATCAAC	34290	0.0	31.047611	2
CAACGCA	34320	0.0	30.777369	5
AACGCAG	34970	0.0	30.230333	6
ATCTCGT	4720	0.0	29.955872	7
CGCAGAG	38750	0.0	27.281748	8
ACGCAGA	39625	0.0	26.678986	7
GTGGTAT	6685	0.0	25.669462	1
GAGTACT	25015	0.0	24.621544	12-13
GCAGAGT	41795	0.0	24.381155	9
TGGTATC	6955	0.0	23.16236	2
GTACATG	25945	0.0	22.541607	1
CAGAGTA	39000	0.0	22.436121	10-11
AGAGTAC	36020	0.0	22.346645	10-11
>>END_MODULE