FastQCFastQC Report
Fri 27 May 2016
SRR2139283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7954675
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA142500.056.082541
GTATCAA257400.039.086951
TCAACGC327700.030.318564
TATCAAC329550.030.2934992
ATCAACG328050.030.2339213
CAACGCA338150.029.304785
AACGCAG341350.029.0678426
GTGGTAT62400.028.0376261
TGGTATC62700.026.8011152
CGCAGAG382250.025.8908148
ACGCAGA392450.025.2059387
CTTATAC46800.024.9223351
GAGTACT235650.024.19793512-13
GCAGAGT413050.023.014859
GTACATG278350.022.9999961
TACATGG278250.022.5425112
CAGAGTA390400.021.90597510-11
GTACTTT262700.021.65898714-15
ACATGGG289550.021.2744623
AGAGTAC356850.021.17749810-11
Produced by FastQC (version 0.11.5)