##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139283_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7954675
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67575633196831	33.0	33.0	33.0	33.0	33.0
2	32.68931829395921	33.0	33.0	33.0	33.0	33.0
3	32.67532991605565	33.0	33.0	33.0	33.0	33.0
4	36.60617825366844	37.0	37.0	37.0	37.0	37.0
5	36.62559513745062	37.0	37.0	37.0	37.0	37.0
6	36.58665024026752	37.0	37.0	37.0	37.0	37.0
7	36.55748701235437	37.0	37.0	37.0	37.0	37.0
8	36.56324224434059	37.0	37.0	37.0	37.0	37.0
9	36.575864884486165	37.0	37.0	37.0	37.0	37.0
10-11	36.51942838645199	37.0	37.0	37.0	37.0	37.0
12-13	36.48482678424951	37.0	37.0	37.0	37.0	37.0
14-15	35.19644473218579	40.0	35.0	40.0	19.5	40.0
16-17	35.48547307086713	40.0	33.0	40.0	24.0	40.0
18-19	37.989010550399605	40.0	37.0	40.0	35.0	40.0
20-21	38.3484400438996	40.0	37.0	40.0	37.0	40.0
22-23	38.393614462956194	40.0	37.0	40.0	37.0	40.0
24-25	38.42610330717165	40.0	37.0	40.0	37.0	40.0
26-27	38.35978157487735	40.0	37.0	40.0	37.0	40.0
28-29	38.28618955051261	40.0	37.0	40.0	37.0	40.0
30-31	38.15247642383263	40.0	37.0	40.0	37.0	40.0
32-33	37.95300506845393	40.0	37.0	40.0	33.0	40.0
34-35	37.863282668473744	40.0	37.0	40.0	33.0	40.0
36-37	37.78651510576911	40.0	37.0	40.0	33.0	40.0
38-39	37.787608081534245	40.0	37.0	40.0	33.0	40.0
40-41	37.725460239900386	40.0	37.0	40.0	33.0	40.0
42-43	37.67410803720533	40.0	37.0	40.0	33.0	40.0
44-45	37.61088463190434	40.0	37.0	40.0	33.0	40.0
46-47	37.451834295895566	40.0	37.0	40.0	33.0	40.0
48-49	37.20969405566561	40.0	37.0	40.0	33.0	40.0
50-51	37.113807925595864	38.5	37.0	40.0	33.0	40.0
52-53	37.023839218234855	37.0	37.0	40.0	33.0	40.0
54-55	36.790398274055576	37.0	37.0	40.0	33.0	40.0
56-57	36.67772985727609	37.0	37.0	40.0	33.0	40.0
58-59	36.61383160197242	37.0	37.0	40.0	33.0	40.0
60-61	36.483296248066566	37.0	37.0	40.0	33.0	40.0
62-63	36.287989490451835	37.0	37.0	40.0	33.0	40.0
64-65	36.0041827482473	37.0	35.0	40.0	33.0	40.0
66-67	35.666477480227044	37.0	33.0	38.5	33.0	40.0
68-69	35.36812592639275	37.0	33.0	37.0	27.0	40.0
70-71	35.131437774926766	37.0	33.0	37.0	27.0	40.0
72-73	34.92627936460457	37.0	33.0	37.0	27.0	40.0
74-75	34.644831743710704	37.0	33.0	37.0	27.0	38.5
76-77	31.70309684018697	33.0	30.0	35.0	24.5	37.0
78-79	33.910426550251245	37.0	33.0	37.0	27.0	37.0
80-81	34.27227855345537	37.0	33.0	37.0	27.0	37.0
82-83	34.267385189623	37.0	33.0	37.0	27.0	37.0
84-85	34.24094911323762	37.0	33.0	37.0	27.0	37.0
86-87	34.06185335246546	37.0	33.0	37.0	27.0	37.0
88-89	33.83715044110701	37.0	33.0	37.0	27.0	37.0
90-91	33.68734402143366	37.0	33.0	37.0	27.0	37.0
92-93	33.71219137573077	33.0	33.0	37.0	27.0	37.0
94-95	33.652024622662736	33.0	33.0	37.0	27.0	37.0
96-97	33.60120036193854	33.0	33.0	37.0	27.0	37.0
98-99	33.60880410017009	33.0	33.0	37.0	27.0	37.0
100	33.65885248029338	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	7.0
20	61.0
21	316.0
22	1304.0
23	3913.0
24	8991.0
25	18156.0
26	32724.0
27	53594.0
28	79352.0
29	113521.0
30	153882.0
31	207048.0
32	276152.0
33	375606.0
34	530306.0
35	830026.0
36	1463854.0
37	2431937.0
38	1351119.0
39	22805.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.710657569291016	19.63997272044427	14.550412179001656	23.09895753126306
2	15.106722831902552	20.843235330796276	40.86399306868558	23.18604876861559
3	18.4153098272856	25.972003288169816	30.296268425084072	25.31641845946051
4	13.01001188865421	17.903015886445186	37.818428809438714	31.268543415461885
5	13.880453590269259	37.419650952120186	34.596805297216676	14.103090160393872
6	30.398413511551382	37.10587798998702	18.324909565758503	14.170798932703097
7	26.92746089563684	32.94779485019815	22.09293277223771	18.031811481927296
8	25.426846477071656	35.55802091223086	20.552040152488946	18.46309245820854
9	26.08559117751511	16.8394434719206	20.687608230380246	36.38735712018404
10-11	24.83012819505511	26.580300263681423	27.803536913827404	20.786034627436067
12-13	25.864255924974938	24.18624268119062	28.18602897038534	21.763472423449105
14-15	23.244796872884717	25.047662765138686	26.231815343646854	25.475725018329744
16-17	22.209662871204667	28.550368179718216	26.66046695810954	22.579501990967575
18-19	22.210963992872117	27.267047365228624	29.04327329526348	21.47871534663578
20-21	23.141190279635875	26.309203818995545	29.083015009321073	21.466590892047506
22-23	22.913791959926307	26.232696358357284	28.968657536967925	21.884854144748484
24-25	22.583321357468712	26.51993533347568	28.97895507777184	21.917788231283765
26-27	22.508256948638618	26.61928461681187	28.968534096118713	21.903924338430794
28-29	22.346481718290853	26.62919821347446	28.96179268197606	22.062527386258626
30-31	22.76027478992958	26.44858430386175	28.847458355787765	21.943682550420903
32-33	22.53272557815809	26.758226015879703	28.72354745305115	21.985500952911057
34-35	22.568017493126234	26.698241991408384	28.813493613206486	21.920246902258896
36-37	22.656702124389454	26.64113102181558	28.705873927181262	21.9962929266137
38-39	22.53857554152653	26.709355632253345	28.684803049319534	22.067265776900584
40-41	22.745397216074117	26.615558502850778	28.537199802889628	22.101844478185473
42-43	22.66583424359611	26.79496023584439	28.266268226344426	22.27293729421508
44-45	22.74280667729376	26.958278940537017	27.99608641981502	22.302827962354204
46-47	22.980996598022532	26.890547783968184	27.52114750188709	22.607308116122198
48-49	22.775264718472187	27.143028267746132	27.442164442713455	22.639542571068223
50-51	22.74154035706609	27.33212078909454	27.322126069866947	22.604212783972425
52-53	23.054309695319834	27.145722155253676	27.148067587337454	22.65190056208904
54-55	22.931989631382223	27.06415541028727	27.21383595658466	22.790019001745847
56-57	22.942485024146396	27.278203571972043	27.11859230454145	22.66071909934011
58-59	22.858685199953214	27.39830215157964	27.140655345723257	22.60235730274389
60-61	22.964158361972657	27.33145255798612	27.123759492583595	22.58062958745763
62-63	22.946650385165775	27.49316328050299	26.949881504056705	22.610304830274526
64-65	22.955368909417658	27.429689132451973	26.954755145649795	22.66018681248058
66-67	23.042403238158467	27.345529087469973	26.827656803676724	22.78441087069483
68-69	22.906120154296822	27.31046986278623	26.99093556837311	22.792474414543843
70-71	22.967331241789783	27.067831016724973	27.009545091704464	22.955292649780784
72-73	23.009833952888506	27.196718545953015	26.86230386896286	22.93114363219562
74-75	22.70630451684435	27.36832931388633	26.960099289856732	22.96526687941259
76-77	22.969319251065105	27.234524572150676	26.83223128406868	22.963924892715546
78-79	22.9379959005754	27.244874634314066	26.824360266158813	22.992769198951716
80-81	22.967158509145822	27.29358494771165	26.796266409598246	22.942990133544278
82-83	22.903381494509905	27.204985871659993	26.92750820686602	22.964124426964084
84-85	23.057764531112348	27.215026470460234	26.8347598721702	22.892449126257215
86-87	22.86129282109899	27.271754963708684	26.9031922524466	22.963759962745726
88-89	22.886743710156406	27.148882083765546	26.910015295643415	23.054358910434637
90-91	22.993276004032275	27.114260608581326	26.957895398327196	22.9345679890592
92-93	23.041565068693494	27.040347259848087	26.93370307067939	22.98438460077903
94-95	22.892418039474265	27.073268071941527	27.005961590413314	23.028352298170898
96-97	22.91060079540791	27.187294180895282	27.072970420513183	22.829134603183622
98-99	23.0808617166583	28.008592190678865	26.058278994039785	22.852267098623045
100	21.151979498763545	27.558374260786	27.34553309704259	23.94411314340786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2023.0
1	1848.5
2	2618.5
3	3841.5
4	5350.0
5	8770.5
6	11630.5
7	13793.5
8	15898.0
9	16300.5
10	15176.0
11	13955.5
12	15036.5
13	19879.5
14	31599.0
15	47721.5
16	61600.5
17	70218.0
18	69779.0
19	61686.5
20	51681.0
21	42229.0
22	35523.0
23	33537.0
24	35904.5
25	42798.5
26	53686.0
27	65463.5
28	77826.5
29	92450.5
30	106353.0
31	119312.5
32	134620.0
33	150856.5
34	165613.0
35	177887.5
36	193618.0
37	209936.5
38	220191.0
39	226933.5
40	235829.0
41	246419.0
42	254351.5
43	263489.0
44	272880.5
45	282883.5
46	293180.0
47	299730.0
48	303200.0
49	305352.5
50	307607.5
51	308579.0
52	305186.5
53	297595.5
54	289611.5
55	277526.5
56	259330.5
57	238255.0
58	215114.0
59	189916.5
60	161238.0
61	133394.0
62	107035.0
63	82375.0
64	62068.5
65	46616.5
66	35760.0
67	27866.0
68	21439.0
69	15391.0
70	10813.5
71	7787.0
72	5383.0
73	3743.0
74	2745.0
75	1943.5
76	1345.5
77	945.5
78	672.5
79	486.0
80	362.0
81	267.0
82	196.0
83	140.5
84	101.5
85	72.5
86	52.0
87	42.5
88	32.0
89	22.0
90	16.5
91	11.5
92	7.0
93	6.0
94	5.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	6.411324158435134E-4
3	0.0012319799363267512
4	4.022791628822045E-4
5	8.799856688048223E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	7.557675203575257
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008496114741193
26-27	0.024181553822456806
28-29	0.009155728488697775
30-31	1.5759314669009636E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.024257910258242168
50-51	0.06777674358976037
52-53	0.05873268329702237
54-55	0.03751323264447654
56-57	0.004868989729015218
58-59	0.009924135644418085
60-61	0.03559970249346724
62-63	0.03671297445956917
64-65	0.0
66-67	0.0032142009140913846
68-69	0.03335813085317709
70-71	0.08665679378315971
72-73	0.07707506271760146
74-75	0.038125786594437105
76-77	0.008363030456842026
78-79	8.608276466537282E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.001436566486255938
92-93	0.002717970494555819
94-95	0.0010506577834081714
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	230.0
20-21	764.0
22-23	1640.0
24-25	4593.0
26-27	5405.0
28-29	7482.0
30-31	12089.0
32-33	12644.0
34-35	16523.0
36-37	18597.0
38-39	21776.0
40-41	29776.0
42-43	37409.0
44-45	51119.0
46-47	51444.0
48-49	32527.0
50-51	28767.0
52-53	27268.0
54-55	37267.0
56-57	38291.0
58-59	44733.0
60-61	49311.0
62-63	47006.0
64-65	43488.0
66-67	67882.0
68-69	36499.0
70-71	47554.0
72-73	49030.0
74-75	49705.0
76-77	40693.0
78-79	61478.0
80-81	75190.0
82-83	82515.0
84-85	91760.0
86-87	97530.0
88-89	99244.0
90-91	105840.0
92-93	115355.0
94-95	138790.0
96-97	318349.0
98-99	63120.0
100-101	5793992.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.43934851755533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.72545500146998	74.03194162246326
2	7.703623710643709	12.855706872810066
3	1.946960622307933	4.873593777441242
4	0.7470832003932242	2.493445421168835
5	0.34369867730094283	1.4338996860168076
6	0.1721999053976886	0.8620948752700678
7	0.10922211516536703	0.6379395493175338
8	0.06684703139082802	0.4462138199666609
9	0.04281307393970258	0.3215065497806439
>10	0.13865662127094344	1.7433811067496072
>50	0.002529809879237909	0.1374524757428596
>100	8.331463678127746E-4	0.12275369142423445
>500	7.708447263624628E-5	0.04007055184820453
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.257122384006889E-5	6.285611920034446E-5	0.0	0.0	0.0
3	1.257122384006889E-5	6.285611920034446E-5	0.0	0.0	0.0
4	1.257122384006889E-5	1.0056979072055112E-4	0.0	1.257122384006889E-5	0.0
5	1.257122384006889E-5	1.0056979072055112E-4	0.0	2.514244768013778E-5	0.0
6	8.799856688048223E-5	1.0056979072055112E-4	0.0	2.514244768013778E-5	0.0
7	8.799856688048223E-5	1.0056979072055112E-4	0.0	2.514244768013778E-5	0.0
8	1.0056979072055112E-4	1.1314101456062001E-4	0.0	3.771367152020667E-5	0.0
9	1.0056979072055112E-4	3.017093721616534E-4	0.0	3.771367152020667E-5	0.0
10-11	1.1314101456062001E-4	3.017093721616534E-4	0.0	5.657050728031001E-5	0.0
12-13	1.257122384006889E-4	3.017093721616534E-4	0.0	1.0056979072055112E-4	0.0
14-15	1.382834622407578E-4	3.897079390421356E-4	0.0	1.1314101456062002E-4	0.0
16-17	1.948539695210678E-4	4.022791628822045E-4	0.0	2.0742519336113669E-4	0.0
18-19	2.1371080528117113E-4	4.337072224823767E-4	0.0	2.891381483215845E-4	0.0
20-21	2.2628202912124002E-4	4.5884967016251446E-4	0.0	3.017093721616534E-4	0.0
22-23	2.388532529613089E-4	5.154201774428245E-4	0.0	3.645654913619978E-4	0.0
24-25	2.891381483215845E-4	5.279914012828934E-4	0.0	4.7770650592261787E-4	0.0
26-27	3.2685181984179115E-4	5.279914012828934E-4	0.0	6.788460873637201E-4	0.0
28-29	3.2685181984179115E-4	5.405626251229624E-4	0.0	0.0012508367720868545	0.0
30-31	3.5199426752192893E-4	6.159899681633756E-4	0.0	0.0021119656051315737	0.0
32-33	3.9599355096217007E-4	6.159899681633756E-4	0.0	0.003488514615619117	0.0
34-35	4.5884967016251446E-4	7.102741469638923E-4	0.0	0.0048839204618667635	0.0
36-37	4.902777297626867E-4	8.04558325764409E-4	0.0	0.006744461590196959	0.0
38-39	4.902777297626867E-4	8.234151615245124E-4	0.0	0.010006694176694836	0.0
40-41	5.342770132029278E-4	0.0010496971906457523	0.0	0.01302378789831137	0.0
42-43	5.405626251229623E-4	0.0011125533098460968	0.0	0.01596545427688749	0.0
44-45	5.405626251229623E-4	0.0013262641151272681	0.0	0.018542555164101613	0.0
46-47	5.531338489630312E-4	0.0014959756369681979	0.0	0.021540792049958044	0.0
48-49	5.531338489630312E-4	0.0014959756369681979	0.0	0.024495029652374233	0.0
50-51	5.78276296643169E-4	0.0017536857256896102	0.0	0.026255000989983877	0.0
52-53	5.971331324032723E-4	0.0017851137852897825	0.0	0.02914638247319972	0.0
54-55	6.034187443233068E-4	0.0017851137852897825	0.0	0.03323831583314214	0.0
56-57	6.034187443233068E-4	0.0017851137852897825	0.0	0.03881993921813273	0.0
58-59	6.411324158435135E-4	0.0018039706210498858	0.0	0.044062139559441466	0.0
60-61	6.662748635236511E-4	0.0018542555164101613	0.0	0.0480472175167433	0.0
62-63	7.291309827239956E-4	0.001879397964090299	0.0	0.05095117022379921	0.0
64-65	7.605590423241679E-4	0.002074251933611367	0.0	0.05330827469381213	0.0
66-67	7.919871019243401E-4	0.00209310876937147	0.0	0.056677362682950594	0.0
68-69	8.297007734445467E-4	0.0021936785600920215	0.0	0.05997730894096868	0.0
70-71	8.422719972846157E-4	0.0022376778435322627	0.0	0.06184413568121891	0.0
72-73	8.799856688048223E-4	0.0022502490673723314	0.0	0.06396867251019056	0.0
74-75	8.799856688048223E-4	0.0022753915150524693	0.0	0.06569093017628	0.0
76-77	8.988425045649256E-4	0.002419960589213261	0.0	0.0676646123191708	0.0
78-79	9.051281164849601E-4	0.0024765310964935715	0.0	0.0691228742846188	0.0
80-81	9.302705641650979E-4	0.0024765310964935715	0.0	0.0697262930289421	0.0
82-83	9.302705641650979E-4	0.002482816708413606	0.0	0.07039256789246576	0.0
84-85	9.428417880051668E-4	0.002501673544173709	0.0	0.07102741469638923	0.0
86-87	9.428417880051668E-4	0.0025331016037738814	0.0	0.0714234082473514	0.0
88	9.428417880051668E-4	0.002627385782574398	0.0	0.0714799787546317	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14250	0.0	56.08254	1
GTATCAA	25740	0.0	39.08695	1
TCAACGC	32770	0.0	30.31856	4
TATCAAC	32955	0.0	30.293499	2
ATCAACG	32805	0.0	30.233921	3
CAACGCA	33815	0.0	29.30478	5
AACGCAG	34135	0.0	29.067842	6
GTGGTAT	6240	0.0	28.037626	1
TGGTATC	6270	0.0	26.801115	2
CGCAGAG	38225	0.0	25.890814	8
ACGCAGA	39245	0.0	25.205938	7
CTTATAC	4680	0.0	24.922335	1
GAGTACT	23565	0.0	24.197935	12-13
GCAGAGT	41305	0.0	23.01485	9
GTACATG	27835	0.0	22.999996	1
TACATGG	27825	0.0	22.542511	2
CAGAGTA	39040	0.0	21.905975	10-11
GTACTTT	26270	0.0	21.658987	14-15
ACATGGG	28955	0.0	21.274462	3
AGAGTAC	35685	0.0	21.177498	10-11
>>END_MODULE