FastQCFastQC Report
Fri 27 May 2016
SRR2139282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9712527
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166250.054.6836661
GTATCAA310800.038.6306231
CTTATAC79550.030.5035321
ATCAACG396100.029.9923083
TCAACGC396550.029.936724
TATCAAC401300.029.7821332
CAACGCA405800.029.202425
AACGCAG412150.028.7933866
GTGGTAT73450.027.0718571
TGGTATC70100.026.562562
CGCAGAG459350.025.8089648
ACGCAGA469250.025.2448147
GAGTACT287450.022.90475712-13
GCAGAGT499600.022.660339
TATACAC125800.022.4367433
GCGAATC27500.022.4119132-33
CAGAGTA471900.021.36926710-11
AGAGTAC441850.020.68833210-11
GTACATG355350.020.6044641
GGCGAAT30650.020.52749432-33
Produced by FastQC (version 0.11.5)