##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139282_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9712527
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61278192585719	33.0	33.0	33.0	33.0	33.0
2	32.636544948600914	33.0	33.0	33.0	33.0	33.0
3	32.61766757508113	33.0	33.0	33.0	33.0	33.0
4	36.5334667280719	37.0	37.0	37.0	37.0	37.0
5	36.55606826112298	37.0	37.0	37.0	37.0	37.0
6	36.5034621782776	37.0	37.0	37.0	37.0	37.0
7	36.47706122206919	37.0	37.0	37.0	37.0	37.0
8	36.48273822044459	37.0	37.0	37.0	37.0	37.0
9	36.504565083834514	37.0	37.0	37.0	37.0	37.0
10-11	36.44178286454184	37.0	37.0	37.0	37.0	37.0
12-13	36.39810983279635	37.0	37.0	37.0	37.0	37.0
14-15	35.25830146984405	40.0	35.0	40.0	19.5	40.0
16-17	35.53596854865886	40.0	33.0	40.0	24.0	40.0
18-19	37.83776009065406	40.0	37.0	40.0	35.0	40.0
20-21	38.152339275130814	40.0	37.0	40.0	37.0	40.0
22-23	38.18528394854143	40.0	37.0	40.0	37.0	40.0
24-25	38.22092703579976	40.0	37.0	40.0	37.0	40.0
26-27	38.1515492259203	40.0	37.0	40.0	37.0	40.0
28-29	38.072294306235136	40.0	37.0	40.0	35.0	40.0
30-31	37.92644776869516	40.0	37.0	40.0	33.0	40.0
32-33	37.71266287866044	40.0	37.0	40.0	33.0	40.0
34-35	37.61783976409539	40.0	37.0	40.0	33.0	40.0
36-37	37.5490637632941	40.0	37.0	40.0	33.0	40.0
38-39	37.54664726440766	40.0	37.0	40.0	33.0	40.0
40-41	37.498858460271	40.0	37.0	40.0	33.0	40.0
42-43	37.440625475088744	40.0	37.0	40.0	33.0	40.0
44-45	37.390549751346626	40.0	37.0	40.0	33.0	40.0
46-47	37.24037728504118	40.0	37.0	40.0	33.0	40.0
48-49	36.992762748886506	37.0	37.0	40.0	33.0	40.0
50-51	36.893330860194254	37.0	37.0	40.0	33.0	40.0
52-53	36.82125213743153	37.0	37.0	40.0	33.0	40.0
54-55	36.590981954368345	37.0	37.0	40.0	33.0	40.0
56-57	36.48283725280353	37.0	37.0	40.0	33.0	40.0
58-59	36.443142682795965	37.0	37.0	40.0	33.0	40.0
60-61	36.32944099915274	37.0	37.0	40.0	33.0	40.0
62-63	36.156294633201234	37.0	37.0	40.0	33.0	40.0
64-65	35.88351673226941	37.0	35.0	40.0	33.0	40.0
66-67	35.541139277796304	37.0	33.0	40.0	27.0	40.0
68-69	35.24812511224228	37.0	33.0	37.0	27.0	40.0
70-71	35.0031920590518	37.0	33.0	37.0	27.0	40.0
72-73	34.779695058750804	37.0	33.0	37.0	27.0	40.0
74-75	34.48884261087567	37.0	33.0	37.0	27.0	40.0
76-77	31.667532844277673	33.0	30.0	35.0	24.5	37.0
78-79	33.82318257182018	37.0	33.0	37.0	27.0	37.0
80-81	34.16300811814416	37.0	33.0	37.0	27.0	37.0
82-83	34.129548409027194	37.0	33.0	37.0	27.0	37.0
84-85	34.073480167282085	37.0	33.0	37.0	27.0	37.0
86-87	33.872168091188406	37.0	33.0	37.0	27.0	37.0
88-89	33.62562102115558	35.0	33.0	37.0	27.0	37.0
90-91	33.45204648378136	33.0	33.0	37.0	27.0	37.0
92-93	33.46344255890328	33.0	33.0	37.0	27.0	37.0
94-95	33.39249658025501	33.0	33.0	37.0	27.0	37.0
96-97	33.35008676364832	33.0	33.0	37.0	27.0	37.0
98-99	33.3534137589215	33.0	33.0	37.0	27.0	37.0
100	33.36418083033485	33.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	1.0
16	0.0
17	3.0
18	5.0
19	17.0
20	107.0
21	453.0
22	1835.0
23	5226.0
24	12154.0
25	24656.0
26	43655.0
27	70896.0
28	105980.0
29	150077.0
30	204286.0
31	274085.0
32	365016.0
33	494788.0
34	695614.0
35	1064866.0
36	1795034.0
37	2803670.0
38	1568154.0
39	31948.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66636036064419	20.57274798921679	14.838874280579816	22.922017369559196
2	14.627925765848952	21.33624890179694	41.83236023593206	22.203465096422047
3	18.106762124246206	26.731386147798837	30.787952321043548	24.37389940691141
4	12.331042401458904	18.73362933688724	39.022203973108454	29.913124288545394
5	13.171164641102411	37.92058085852075	35.39286405587839	13.51539044449844
6	28.92836230982936	38.368263995559545	18.96353029443316	13.739843400177934
7	25.79658208414762	33.50963142753683	22.805733255619266	17.88805323269629
8	24.360673591949862	36.44033113112582	21.207807195799816	17.99118808112451
9	25.43381346584674	17.131205915823966	21.36550045111844	36.06948016721086
10-11	23.85017308059993	27.314595882204497	28.607853548309315	20.227377488886262
12-13	25.051863433687238	24.83953455161566	28.926210449659497	21.182391565037605
14-15	22.35381480102745	25.834852869212632	27.02379573541986	24.78753659434006
16-17	21.26880573922729	29.420968405029917	27.510796108983786	21.799429746759007
18-19	21.205089056637885	28.01935582778817	29.903458698235795	20.872096417338145
20-21	22.22823478983279	27.069471171654598	29.882611467417664	20.81968257109495
22-23	21.87289491709317	27.1717350159288	29.708093625592063	21.24727644138597
24-25	21.616142022369957	27.34771332732992	29.847332298060024	21.188812352240095
26-27	21.557943898643483	27.53433041314013	29.7858160748348	21.121909613381593
28-29	21.318894144355202	27.52693500265891	29.852909921000215	21.30126093198567
30-31	21.689704755650695	27.495253603512136	29.69026703140915	21.124774609428012
32-33	21.635148056658497	27.496848104491423	29.63627563216876	21.231728206681318
34-35	21.600956697440076	27.569659311753174	29.62643527065534	21.20294872015141
36-37	21.57703753231883	27.603888900781904	29.515040590507386	21.30403297639188
38-39	21.425853351730186	27.562094868891045	29.6378493589814	21.37420242039737
40-41	21.709519999915898	27.484738165047535	29.443588242158302	21.362153592878265
42-43	21.74255559388784	27.528298798838353	29.236181838051646	21.49296376922217
44-45	21.76590025830135	27.83249772353423	28.887135885347533	21.514466132816885
46-47	21.961592474137102	27.705136356283223	28.374430382059863	21.95884078751981
48-49	21.87621387141926	27.917776937226307	28.297943984363577	21.90806520699086
50-51	21.77395950218067	28.096365686463304	28.22152394336383	21.908150867992198
52-53	21.918810283949274	28.028942732651746	28.170710780693113	21.881536202705863
54-55	21.965747280386125	27.96368995977765	28.089283301983503	21.981279457852725
56-57	22.07023667034363	28.120972040347397	27.876770180063897	21.932021109245078
58-59	21.896367499401645	28.194465716438927	28.095118382850497	21.814048401308927
60-61	22.041720958703902	28.248839393110202	27.87614667085179	21.833292977334104
62-63	21.921135619202918	28.422797217997086	27.761808998909082	21.894258163890914
64-65	21.964349428015765	28.338390651921525	27.78911062367357	21.908149296389144
66-67	22.02718992798496	28.218411436825207	27.689638588774322	22.064760046415504
68-69	22.032852881994323	28.186863919858634	27.835535279086347	21.944747919060696
70-71	22.038455619759755	27.938907576586665	27.838765521795427	22.183871281858156
72-73	22.04900510061028	28.028434068094832	27.803645536824913	22.118915294469975
74-75	21.89940043360973	28.16827495072603	27.781586174282314	22.15073844138193
76-77	22.063468325985944	28.092900553064272	27.679378141986493	22.16425297896329
78-79	22.078083886519213	28.106113151090597	27.658647832971305	22.157155129418886
80-81	22.03001281088649	28.06722498174666	27.678710680729456	22.2240515266374
82-83	22.081826554384918	28.007248444075067	27.811962570954517	22.0989624305855
84-85	22.225496178918043	27.95144430003856	27.763147815740357	22.059911705303044
86-87	21.97844074754567	27.9696948423	27.85460149088685	22.197262919267484
88-89	22.061341400744524	27.94888666814493	27.787656897593294	22.20211503351725
90-91	22.14726329834024	27.89529370863082	27.850828181271996	22.106614811756938
92-93	22.102195533164423	27.832705513581217	27.880664249150744	22.184434704103612
94-95	21.99294337857972	27.8982761922699	27.899754177847385	22.209026251302994
96-97	21.980211511678064	28.06787930488316	28.011705998143583	21.9402031852952
98-99	23.051399889737922	29.989928549740615	28.180640093516622	18.77803146700484
100	22.05346107715659	30.9860014277865	22.062352115120834	24.898185379936074
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2203.0
1	2036.5
2	2920.0
3	4236.5
4	6012.5
5	10263.5
6	13775.0
7	16032.0
8	17849.0
9	17980.0
10	16830.0
11	15671.5
12	17281.0
13	23684.5
14	38672.0
15	58708.5
16	76035.5
17	87649.5
18	88673.0
19	80335.0
20	69366.0
21	58800.0
22	52107.0
23	52165.5
24	58223.0
25	70041.5
26	87694.0
27	108022.0
28	128346.5
29	150522.0
30	171532.5
31	190930.5
32	213637.0
33	236286.5
34	258043.5
35	278380.5
36	299731.5
37	318052.5
38	328483.0
39	336683.5
40	346969.5
41	356749.5
42	362909.0
43	369721.0
44	375071.5
45	380614.5
46	386194.0
47	387948.0
48	385064.5
49	377856.0
50	370733.5
51	361552.5
52	345579.5
53	325924.0
54	305663.5
55	283375.0
56	259046.5
57	232860.5
58	205683.5
59	178994.0
60	151264.5
61	123021.0
62	97169.0
63	74841.5
64	56222.0
65	42037.5
66	31631.5
67	24155.5
68	18226.5
69	12959.5
70	9235.5
71	6693.5
72	4738.0
73	3332.5
74	2354.5
75	1653.5
76	1197.5
77	851.5
78	597.5
79	421.0
80	306.0
81	223.5
82	161.0
83	104.0
84	66.0
85	44.5
86	33.0
87	28.0
88	25.0
89	23.5
90	19.0
91	14.5
92	11.0
93	5.5
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	1.8532767013157338E-4
2	7.207187171783409E-4
3	0.001544397251096445
4	3.1917543189326523E-4
5	7.20718717178341E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	6.971898250578866
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.008462022515057302
26-27	0.023884253660358495
28-29	0.008962789211751566
30-31	1.0328346394560185E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.025291454615224418
50-51	0.06804604907763984
52-53	0.05839403536270202
54-55	0.038427643877309924
56-57	0.005092980398726755
58-59	0.00840920202136264
60-61	0.03170242775918681
62-63	0.03295972862087144
64-65	0.0
66-67	0.0026743323073940618
68-69	0.031234265303979217
70-71	0.08329799929082288
72-73	0.07536499407015124
74-75	0.03710117673857998
76-77	0.00772147477965652
78-79	6.667945101079256E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0012656694035546258
92-93	0.0024862859650241213
94-95	8.080510058370289E-4
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	272.0
20-21	913.0
22-23	2162.0
24-25	4647.0
26-27	6598.0
28-29	10885.0
30-31	21356.0
32-33	25411.0
34-35	31964.0
36-37	37441.0
38-39	45502.0
40-41	56908.0
42-43	65092.0
44-45	79086.0
46-47	80015.0
48-49	66944.0
50-51	64869.0
52-53	72206.0
54-55	68304.0
56-57	75320.0
58-59	80501.0
60-61	73876.0
62-63	67479.0
64-65	86783.0
66-67	89765.0
68-69	66302.0
70-71	77504.0
72-73	81477.0
74-75	84096.0
76-77	69989.0
78-79	83724.0
80-81	92171.0
82-83	86939.0
84-85	95785.0
86-87	105669.0
88-89	117389.0
90-91	124351.0
92-93	135058.0
94-95	162756.0
96-97	371488.0
98-99	613762.0
100-101	6129768.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.10599423166727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.21971790521876	77.68458509529414
2	6.889714134981275	11.86491371129068
3	1.669862417289388	4.313554910123939
4	0.5799962516204702	1.997646155856834
5	0.26933799187799645	1.1595807787507804
6	0.13112126526071952	0.6774196146113057
7	0.07418688528681504	0.44715548616003
8	0.04559579506772923	0.3140857013672141
9	0.029096938479755446	0.22548787352073002
>10	0.08966762399251388	1.1426258373913696
>50	0.0012242017120336075	0.06896663674544327
>100	4.240744777093735E-4	0.06068349803084592
>500	3.63432505168472E-5	0.023360757682443452
>1k	1.817148425616393E-5	0.01993394317422615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.0591963347952597E-5	0.0	0.0	0.0
3	0.0	2.0591963347952597E-5	0.0	1.0295981673976299E-5	0.0
4	0.0	4.1183926695905195E-5	0.0	1.0295981673976299E-5	0.0
5	0.0	4.1183926695905195E-5	0.0	1.0295981673976299E-5	0.0
6	5.14799083698815E-5	4.1183926695905195E-5	0.0	1.0295981673976299E-5	1.0295981673976299E-5
7	5.14799083698815E-5	5.14799083698815E-5	0.0	1.0295981673976299E-5	1.0295981673976299E-5
8	5.14799083698815E-5	6.17758900438578E-5	0.0	2.0591963347952597E-5	1.0295981673976299E-5
9	5.14799083698815E-5	1.5443972510964448E-4	0.0	7.207187171783409E-5	1.0295981673976299E-5
10-11	5.14799083698815E-5	1.5443972510964448E-4	0.0	7.207187171783409E-5	1.0295981673976299E-5
12-13	6.17758900438578E-5	1.5443972510964448E-4	0.0	7.721986255482224E-5	1.0295981673976299E-5
14-15	7.207187171783409E-5	1.9047566096856152E-4	0.0	8.236785339181039E-5	2.0591963347952597E-5
16-17	8.236785339181039E-5	2.05919633479526E-4	0.0	8.236785339181039E-5	3.08879450219289E-5
18-19	8.236785339181039E-5	2.2136360599049043E-4	0.0	9.266383506578669E-5	4.1183926695905195E-5
20-21	8.236785339181039E-5	2.3680757850145487E-4	0.0	1.02959816739763E-4	4.1183926695905195E-5
22-23	8.236785339181039E-5	2.7284351436037194E-4	0.0	1.3899575259868004E-4	4.1183926695905195E-5
24-25	9.266383506578669E-5	3.037314593823008E-4	0.0	3.191754318932653E-4	4.1183926695905195E-5
26-27	9.266383506578669E-5	3.191754318932653E-4	0.0	8.28826524755092E-4	4.1183926695905195E-5
28-29	9.266383506578669E-5	3.191754318932653E-4	0.0	0.0027747670611366123	4.1183926695905195E-5
30-31	9.781182590277484E-5	3.5006337691519416E-4	0.0	0.007557250548698604	4.6331917532893346E-5
32-33	1.0810780757675113E-4	3.552113677521823E-4	0.0	0.016900853917832095	5.14799083698815E-5
34-35	1.235517800877156E-4	4.169872577960401E-4	0.0	0.02976568301946548	6.17758900438578E-5
36-37	1.235517800877156E-4	4.993551111878505E-4	0.0	0.04515302763122306	6.17758900438578E-5
38-39	1.235517800877156E-4	5.250950653727912E-4	0.0	0.06489557249107261	7.207187171783409E-5
40-41	1.235517800877156E-4	5.559830103947202E-4	0.0	0.08051972468133164	7.207187171783409E-5
42-43	1.235517800877156E-4	5.81722964579661E-4	0.0	0.09429060017027494	7.721986255482224E-5
44-45	1.235517800877156E-4	6.795347904824357E-4	0.0	0.10515286083631994	8.236785339181039E-5
46-47	1.235517800877156E-4	7.516066622002698E-4	0.0	0.11326094640457628	8.236785339181039E-5
48-49	1.2869977092470374E-4	7.516066622002698E-4	0.0	0.11852219303997816	9.266383506578669E-5
50-51	1.5443972510964448E-4	8.64862460614009E-4	0.0	0.12349000419767173	9.266383506578669E-5
52-53	1.6473570678362078E-4	8.751584422879854E-4	0.0	0.13351314235728765	9.266383506578669E-5
54-55	1.6988369762060892E-4	8.803064331249736E-4	0.0	0.14368557225117623	9.266383506578669E-5
56-57	1.7503168845759708E-4	8.854544239619617E-4	0.0	0.15011026481573747	9.781182590277484E-5
58-59	1.7503168845759708E-4	8.854544239619617E-4	0.0	0.15788887897042653	1.02959816739763E-4
60-61	1.8532767013157338E-4	9.008983964729261E-4	0.0	0.1639120282497027	1.02959816739763E-4
62-63	2.3680757850145487E-4	9.163423689838906E-4	0.0	0.16700082275189557	1.02959816739763E-4
64-65	2.3680757850145487E-4	0.001003858213212689	0.0	0.16974470186801024	1.0810780757675113E-4
66-67	2.4195556933844303E-4	0.0010090062040496773	0.0	0.17334314746306495	1.1840378925072743E-4
68-69	2.471035601754312E-4	0.0010450421399085942	0.0	0.17722473255415405	1.235517800877156E-4
70-71	2.5739954184940747E-4	0.0010604861124195587	0.0	0.17943322062322195	1.3384776176169187E-4
72-73	2.8828748687133635E-4	0.0010656341032565467	0.0	0.18152330490303914	1.4414374343566818E-4
74-75	2.8828748687133635E-4	0.0011171140116264283	0.0	0.18365457310955224	1.4414374343566818E-4
76-77	2.8828748687133635E-4	0.00116859391999631	0.0	0.18586306117862017	1.4414374343566818E-4
78-79	2.8828748687133635E-4	0.0011840378925072744	0.0	0.18706769103447538	1.4414374343566818E-4
80-81	2.8828748687133635E-4	0.0011840378925072744	0.0	0.18755675016398926	1.5443972510964448E-4
82-83	2.985834685453127E-4	0.0011891858833442625	0.0	0.18836498472539637	1.6473570678362078E-4
84-85	2.985834685453127E-4	0.0012149258375292033	0.0	0.1888900797907692	1.6473570678362078E-4
86-87	2.985834685453127E-4	0.001245813782551132	0.0	0.18915262732345559	1.6473570678362078E-4
88	2.985834685453127E-4	0.00130758967259499	0.0	0.18916807129596655	1.7503168845759708E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16625	0.0	54.683666	1
GTATCAA	31080	0.0	38.630623	1
CTTATAC	7955	0.0	30.503532	1
ATCAACG	39610	0.0	29.992308	3
TCAACGC	39655	0.0	29.93672	4
TATCAAC	40130	0.0	29.782133	2
CAACGCA	40580	0.0	29.20242	5
AACGCAG	41215	0.0	28.793386	6
GTGGTAT	7345	0.0	27.071857	1
TGGTATC	7010	0.0	26.56256	2
CGCAGAG	45935	0.0	25.808964	8
ACGCAGA	46925	0.0	25.244814	7
GAGTACT	28745	0.0	22.904757	12-13
GCAGAGT	49960	0.0	22.66033	9
TATACAC	12580	0.0	22.436743	3
GCGAATC	2750	0.0	22.41191	32-33
CAGAGTA	47190	0.0	21.369267	10-11
AGAGTAC	44185	0.0	20.688332	10-11
GTACATG	35535	0.0	20.604464	1
GGCGAAT	3065	0.0	20.527494	32-33
>>END_MODULE