FastQCFastQC Report
Fri 27 May 2016
SRR2139281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8174355
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124400.052.274421
CTTATAC133750.044.1770061
GTATCAA222750.036.5114141
TATACAC180950.035.0463523
TTATACA179100.033.8915372
TCAACGC267700.029.9046574
ATCAACG268750.029.8197753
TATCAAC276700.029.1931172
CAACGCA274750.029.1280445
AACGCAG284350.028.1436146
TGGTATC51250.026.0896052
TACACAT242500.025.58255
GTGGTAT57150.025.3273051
ACACATC237500.025.1017576
ACGCAGA322300.024.790287
ACATCTC243500.024.6052138
CGCAGAG326200.024.5068998
CACATCT260350.022.8008777
ATACACA302750.021.7877484
CATCTCC275700.021.5006249
Produced by FastQC (version 0.11.5)