##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139281_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8174355
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.754297433864814	33.0	33.0	33.0	33.0	33.0
2	32.762478140477135	33.0	33.0	33.0	33.0	33.0
3	32.761208193184665	33.0	33.0	33.0	33.0	33.0
4	36.6911850537443	37.0	37.0	37.0	37.0	37.0
5	36.71417169917382	37.0	37.0	37.0	37.0	37.0
6	36.719307272561565	37.0	37.0	37.0	37.0	37.0
7	36.70146280654559	37.0	37.0	37.0	37.0	37.0
8	36.69493837740103	37.0	37.0	37.0	37.0	37.0
9	36.69790289264413	37.0	37.0	37.0	37.0	37.0
10-11	36.6455377824917	37.0	37.0	37.0	37.0	37.0
12-13	36.64188611823196	37.0	37.0	37.0	37.0	37.0
14-15	38.83632476201487	40.0	37.0	40.0	37.0	40.0
16-17	38.76497882707565	40.0	37.0	40.0	37.0	40.0
18-19	38.78895931238611	40.0	37.0	40.0	37.0	40.0
20-21	38.8402880532993	40.0	37.0	40.0	37.0	40.0
22-23	38.87650261774252	40.0	38.5	40.0	37.0	40.0
24-25	38.88545114682879	40.0	40.0	40.0	37.0	40.0
26-27	38.7709513940262	40.0	37.0	40.0	37.0	40.0
28-29	38.67177947215531	40.0	37.0	40.0	37.0	40.0
30-31	38.60792275778887	40.0	37.0	40.0	37.0	40.0
32-33	38.54396922356912	40.0	37.0	40.0	37.0	40.0
34-35	38.520509705026264	40.0	37.0	40.0	37.0	40.0
36-37	38.47648845684651	40.0	37.0	40.0	37.0	40.0
38-39	38.40751776485095	40.0	37.0	40.0	37.0	40.0
40-41	38.379442755578616	40.0	37.0	40.0	37.0	40.0
42-43	38.27218887083024	40.0	37.0	40.0	37.0	40.0
44-45	38.230578442027806	40.0	37.0	40.0	37.0	40.0
46-47	38.158729324048196	40.0	37.0	40.0	37.0	40.0
48-49	38.098416314508015	40.0	37.0	40.0	37.0	40.0
50-51	37.99432873123706	40.0	37.0	40.0	33.0	40.0
52-53	37.95998038574622	40.0	37.0	40.0	33.0	40.0
54-55	37.90103530180474	40.0	37.0	40.0	33.0	40.0
56-57	37.79019040338457	40.0	37.0	40.0	33.0	40.0
58-59	37.54291445456661	40.0	37.0	40.0	33.0	40.0
60-61	37.46267869711106	38.5	37.0	40.0	33.0	40.0
62-63	37.10666624993598	37.0	37.0	40.0	33.0	40.0
64-65	36.931063216557895	37.0	37.0	40.0	33.0	40.0
66-67	36.78997452576063	37.0	37.0	40.0	33.0	40.0
68-69	36.5699537010116	37.0	37.0	40.0	33.0	40.0
70-71	36.29558543354149	37.0	37.0	40.0	33.0	40.0
72-73	36.05612412031313	37.0	37.0	37.0	33.0	40.0
74-75	35.804164875150036	37.0	37.0	37.0	33.0	40.0
76-77	34.003420221792524	35.0	33.0	37.0	30.0	37.0
78-79	35.41084520220127	37.0	33.0	37.0	33.0	37.0
80-81	35.57098877206276	37.0	37.0	37.0	33.0	37.0
82-83	35.514456298206056	37.0	35.0	37.0	33.0	37.0
84-85	35.07462986957998	37.0	33.0	37.0	33.0	37.0
86-87	35.16149827408503	37.0	33.0	37.0	33.0	37.0
88-89	35.15986443300707	37.0	33.0	37.0	33.0	37.0
90-91	35.09679819875609	37.0	33.0	37.0	33.0	37.0
92-93	35.015245829433596	37.0	33.0	37.0	33.0	37.0
94-95	34.96079284121949	37.0	33.0	37.0	33.0	37.0
96-97	34.924456499485046	37.0	33.0	37.0	33.0	37.0
98-99	34.89546843866483	37.0	33.0	37.0	33.0	37.0
100	34.82271401961283	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	3.0
20	37.0
21	230.0
22	797.0
23	2281.0
24	5376.0
25	10880.0
26	19172.0
27	30996.0
28	46191.0
29	64381.0
30	87556.0
31	116148.0
32	155065.0
33	210460.0
34	297844.0
35	457887.0
36	873514.0
37	2398917.0
38	3269367.0
39	127249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.291869511417104	20.157076613384177	14.51397694374663	24.037076931452084
2	14.230688537505406	20.583825879840063	42.542671562465785	22.642814020188748
3	18.09588099617115	26.039757981703048	30.80773814577421	25.056622876351593
4	12.075998545238814	18.457674134473386	37.97646243392511	31.48986488636269
5	13.194763454184383	37.65522529696298	35.38170923104458	13.768302017808054
6	29.579813942506778	37.644768302820225	18.720327169544262	14.055090585128735
7	26.164645406273646	32.925704841544075	22.120656614497413	18.78899313768487
8	24.390915246524038	36.67092999998165	21.15973676210539	17.778417991388924
9	25.00739202053251	16.96526514936041	20.704410317388955	37.322932512718126
10-11	23.864359940325567	26.843371740033312	28.470246276311713	20.82202204332941
12-13	24.93084775496048	24.295078938949924	28.578260914775537	22.195812391314053
14-15	22.194766437229603	25.845556010229554	26.11371172404428	25.84596582849656
16-17	21.744223244525106	29.3161050627236	26.730390348840977	22.209281343910313
18-19	21.629841131196283	27.79276163073417	28.646394730838093	21.931002507231455
20-21	22.577346419657257	27.021900966855096	28.59454141132768	21.80621120215997
22-23	22.180363463035064	27.59084648953081	28.46451971095903	21.764270336475093
24-25	22.175678944053864	27.754017687166314	28.58169523108764	21.488608137692182
26-27	22.070814791758135	27.947047446942726	28.56160641740185	21.420531343897288
28-29	21.605107337998636	27.408054770157225	28.786017454892672	22.20082043695147
30-31	22.47945768938058	27.43757177016172	28.724513999890544	21.358456540567154
32-33	22.33550919888229	27.52654106463219	28.922111257900735	21.215838478584782
34-35	22.000874643670823	27.744844615182974	29.084027967646442	21.17025277349976
36-37	22.084711695554912	27.356461872435922	28.758714502757858	21.800111929251308
38-39	21.66572249876607	27.117134730851166	29.131049840864563	22.0860929295182
40-41	21.70987093977442	27.022397610641008	29.304982341733865	21.96274910785071
42-43	21.978589727780044	26.922268009571717	29.307827165406653	21.791315097241586
44-45	21.660561123459786	27.469445850520106	28.88681461386648	21.98317841215363
46-47	22.33829246286749	26.997897760306174	28.12455962698721	22.539250149839123
48-49	22.01613989837084	27.12574369926336	28.423298965080505	22.434817437285293
50-51	21.781273945394172	27.594643686007313	28.43810250030467	22.185979868293845
52-53	21.820297946381114	27.776254767495047	28.67933231375262	21.72411497237122
54-55	21.783786868201826	27.839248225558812	28.15026777310825	22.226697133131115
56-57	22.197190620325998	28.145884656971166	27.622786990222508	22.034137732480335
58-59	21.677585231044148	28.481658932130237	28.22514503353088	21.615610803294732
60-61	22.137718273318004	28.847032268732743	27.453219642292893	21.56202981565636
62-63	21.762247233681588	29.06498861701351	27.429987858929156	21.742776290375748
64-65	21.80836469338171	28.801350269747694	27.543083484735693	21.847201552134905
66-67	21.972126141192135	28.509259664266906	27.50265536490413	22.015958829636833
68-69	22.054024686692035	28.16601385226352	27.670898507402857	22.109062953641587
70-71	22.053347544077983	27.882200984496947	27.792860951371956	22.27159052005311
72-73	22.081866607721736	27.869180752689953	27.75617217122842	22.292780468359894
74-75	22.01445164870171	28.02499062395579	27.66300433979005	22.29755338755245
76-77	22.1979339068237	27.99878513098224	27.556544624742312	22.24673633745175
78-79	22.165434330849205	28.0071490491603	27.52625600311976	22.30116061687073
80-81	22.122690173136526	28.037830083016207	27.5092320575917	22.330247686255568
82-83	22.14911222430422	27.976771909232905	27.669748035807277	22.204367830655595
84-85	22.323465254836655	27.933194376232006	27.52084876050404	22.222491608427294
86-87	22.087054746284867	28.007821619225894	27.638640881012027	22.266482753477217
88-89	22.188718687480765	28.040230704235007	27.57371067458545	22.197339933698775
90-91	22.21006786666476	28.050304440437053	27.586340069246518	22.153287623651668
92-93	22.22018757797259	28.006563359343524	27.633699197498686	22.139549865185202
94-95	22.106547060938084	28.111007075183725	27.65341338553809	22.129032478340104
96-97	22.180110176305934	28.1741923561462	27.763159027714302	21.882538439833564
98-99	23.27136762539442	30.082646347172037	27.94953515184167	18.696450875591868
100	22.190086595267474	31.097567732908736	21.89431068547997	24.81803498634382
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2529.0
1	2116.0
2	2361.0
3	3287.0
4	4426.5
5	7011.0
6	9316.0
7	11157.0
8	12704.5
9	12834.0
10	11837.5
11	10932.0
12	11877.0
13	15490.5
14	24047.0
15	35679.5
16	45219.0
17	50893.5
18	52191.5
19	49450.0
20	44472.0
21	39207.0
22	35801.5
23	36553.5
24	42006.5
25	51487.5
26	64655.0
27	80368.0
28	96402.0
29	113326.0
30	130412.0
31	147657.0
32	166163.0
33	184641.0
34	204136.0
35	222518.5
36	241583.0
37	258734.0
38	270253.0
39	281499.5
40	294451.0
41	305594.0
42	312064.0
43	318774.0
44	325810.0
45	331603.5
46	340021.0
47	353385.0
48	380912.5
49	383613.0
50	362112.5
51	352423.0
52	335188.5
53	304647.0
54	271648.0
55	245616.0
56	223981.0
57	202055.5
58	178870.5
59	155630.5
60	131454.5
61	107836.5
62	85169.0
63	64789.5
64	48258.5
65	34956.0
66	25539.5
67	19089.0
68	14217.0
69	10063.5
70	6967.0
71	4865.0
72	3288.0
73	2129.0
74	1395.5
75	945.5
76	625.5
77	402.0
78	263.0
79	177.5
80	119.5
81	88.0
82	68.5
83	43.5
84	25.5
85	17.5
86	15.5
87	12.5
88	8.0
89	6.0
90	5.5
91	4.5
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001186637967154595
4	0.031403089295730366
5	0.002299875647681071
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.505134639077868E-5
22-23	0.0
24-25	0.0031447539416645694
26-27	0.040465237625253586
28-29	0.08805180483237857
30-31	0.08405330785720955
32-33	0.038449363822159216
34-35	0.0021813238380429902
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.985906813705855E-5
56-57	1.069154303623384E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.4457483648947428E-5
72-73	0.011761835447182359
74-75	0.0254645796741328
76-77	0.003797248581909359
78-79	0.0
80-81	0.0
82-83	0.004596798981294459
84-85	0.04305246268780156
86-87	0.04350959936845463
88-89	0.010400894884838327
90-91	9.516232789893E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	413.0
22-23	940.0
24-25	2197.0
26-27	3590.0
28-29	7097.0
30-31	17036.0
32-33	21999.0
34-35	29915.0
36-37	43749.0
38-39	49365.0
40-41	52707.0
42-43	61582.0
44-45	73529.0
46-47	71493.0
48-49	64291.0
50-51	54469.0
52-53	51761.0
54-55	65552.0
56-57	78901.0
58-59	74783.0
60-61	78551.0
62-63	81385.0
64-65	86641.0
66-67	98347.0
68-69	71362.0
70-71	61313.0
72-73	57405.0
74-75	58101.0
76-77	57970.0
78-79	61347.0
80-81	62459.0
82-83	60211.0
84-85	59551.0
86-87	63022.0
88-89	68081.0
90-91	73337.0
92-93	77595.0
94-95	93474.0
96-97	262762.0
98-99	492010.0
100-101	5323963.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.9509920410179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.7909745259111	77.17623336831825
2	7.371369798750366	12.671530938075845
3	1.6805733540267715	4.333408409289053
4	0.560781733198998	1.9279898514774119
5	0.25972803853590354	1.1161941286514316
6	0.12485203485671423	0.6438693752564599
7	0.06438248229019326	0.38736167560337637
8	0.039904296362979194	0.2743851087277475
9	0.025015187519609034	0.19350721650622377
>10	0.08040336984669628	1.033812308767628
>50	0.0015178577604843383	0.08358956046474933
>100	3.9015985508338946E-4	0.06617803226844415
>500	6.42968933802771E-5	0.03418988127133671
>1k	4.2864191673012065E-5	0.057750145322090746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.893352441874619E-5	0.0	0.0	0.0
3	0.0	4.893352441874619E-5	0.0	1.2233381104686547E-5	0.0
4	0.0	8.563366773280583E-5	0.0	2.4466762209373094E-5	0.0
5	2.4466762209373094E-5	8.563366773280583E-5	0.0	2.4466762209373094E-5	0.0
6	2.4466762209373094E-5	8.563366773280583E-5	0.0	2.4466762209373094E-5	0.0
7	2.4466762209373094E-5	8.563366773280583E-5	0.0	2.4466762209373094E-5	0.0
8	2.4466762209373094E-5	8.563366773280583E-5	0.0	4.893352441874619E-5	1.2233381104686547E-5
9	2.4466762209373094E-5	1.590339543609251E-4	0.0	9.786704883749238E-5	1.2233381104686547E-5
10-11	2.4466762209373094E-5	1.590339543609251E-4	0.0	1.1010042994217892E-4	1.2233381104686547E-5
12-13	3.670014331405964E-5	1.590339543609251E-4	0.0	1.1010042994217892E-4	1.2233381104686547E-5
14-15	4.2816833866402914E-5	2.1408416933201458E-4	0.0	1.2233381104686546E-4	3.670014331405964E-5
16-17	5.5050214971089456E-5	2.2020085988435785E-4	0.0	1.34567192151552E-4	4.893352441874619E-5
18-19	6.116690552343273E-5	2.2020085988435785E-4	0.0	1.6515064491326838E-4	4.893352441874619E-5
20-21	6.116690552343273E-5	2.324342409890444E-4	0.0	2.0185078822732803E-4	4.893352441874619E-5
22-23	8.563366773280583E-5	2.446676220937309E-4	0.0	2.69134384303104E-4	7.951697718046255E-5
24-25	9.786704883749238E-5	2.69134384303104E-4	0.0	2.8748445596013387E-4	1.2233381104686546E-4
26-27	1.1010042994217892E-4	2.8136776540779057E-4	0.0	4.2816833866402915E-4	1.34567192151552E-4
28-29	1.1010042994217892E-4	2.8136776540779057E-4	0.0	0.0011621712049452219	1.34567192151552E-4
30-31	1.1010042994217892E-4	3.3030128982653676E-4	0.0	0.003529330448702069	1.34567192151552E-4
32-33	1.1010042994217892E-4	3.3641798037888006E-4	0.0	0.0075663462132486295	1.34567192151552E-4
34-35	1.1621712049452219E-4	4.0981826700699935E-4	0.0	0.013071367710357575	1.34567192151552E-4
36-37	1.2233381104686546E-4	4.7710186308277534E-4	0.0	0.022093486275063905	1.34567192151552E-4
38-39	1.2233381104686546E-4	5.076853158444917E-4	0.0	0.04397900507134814	1.34567192151552E-4
40-41	1.2233381104686546E-4	5.872022930249542E-4	0.0	0.0676077806750502	1.34567192151552E-4
42-43	1.2845050159920873E-4	6.055523646819841E-4	0.0	0.08219608764238891	1.34567192151552E-4
44-45	1.34567192151552E-4	6.606025796530735E-4	0.0	0.10023420808124922	1.34567192151552E-4
46-47	1.4068388270389528E-4	6.973027229671332E-4	0.0	0.11974645094322427	1.34567192151552E-4
48-49	1.4680057325623856E-4	6.973027229671332E-4	0.0	0.1390446096358673	1.4680057325623856E-4
50-51	1.4680057325623856E-4	7.82936390699939E-4	0.0	0.15621416001629487	1.4680057325623856E-4
52-53	1.4680057325623856E-4	7.82936390699939E-4	0.0	0.19429667539518408	1.590339543609251E-4
54-55	1.4680057325623856E-4	7.82936390699939E-4	0.0	0.23889346621231888	1.7126733546561165E-4
56-57	1.4680057325623856E-4	7.951697718046256E-4	0.0	0.28487262909428324	1.7126733546561165E-4
58-59	1.5291726380858183E-4	7.951697718046256E-4	0.0	0.31507073035120203	1.7126733546561165E-4
60-61	1.590339543609251E-4	7.951697718046256E-4	0.0	0.33745170108222605	1.7126733546561165E-4
62-63	1.590339543609251E-4	7.951697718046256E-4	0.0	0.35375879809477323	1.7126733546561165E-4
64-65	1.590339543609251E-4	8.685700584327448E-4	0.0	0.36120892718752734	1.7126733546561165E-4
66-67	1.590339543609251E-4	8.930368206421179E-4	0.0	0.3676926191730112	1.7126733546561165E-4
68-69	1.7126733546561165E-4	9.17503582851491E-4	0.0	0.373943876917506	1.7126733546561165E-4
70-71	1.7126733546561165E-4	9.17503582851491E-4	0.0	0.3787026621672291	1.7126733546561165E-4
72-73	1.7126733546561165E-4	9.17503582851491E-4	0.0	0.38236655980808276	1.7126733546561165E-4
74-75	1.7126733546561165E-4	9.419703450608641E-4	0.0	0.38484381948178176	1.7126733546561165E-4
76-77	1.7126733546561165E-4	0.001033720703346013	0.0	0.38727826232161433	1.7126733546561165E-4
78-79	1.7126733546561165E-4	0.001076537537212416	0.0	0.3888380184124619	1.7126733546561165E-4
80-81	1.7126733546561165E-4	0.001076537537212416	0.0	0.3894925043015626	1.7126733546561165E-4
82-83	1.7126733546561165E-4	0.001076537537212416	0.0	0.38998795623630245	1.7126733546561165E-4
84-85	1.7126733546561165E-4	0.0010887709183171027	0.0	0.39029379076391957	1.7126733546561165E-4
86-87	1.7126733546561165E-4	0.0010887709183171027	0.0	0.39040389119386176	1.7126733546561165E-4
88	1.7126733546561165E-4	0.001186637967154595	0.0	0.39040389119386176	1.835007165702982E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12440	0.0	52.27442	1
CTTATAC	13375	0.0	44.177006	1
GTATCAA	22275	0.0	36.511414	1
TATACAC	18095	0.0	35.046352	3
TTATACA	17910	0.0	33.891537	2
TCAACGC	26770	0.0	29.904657	4
ATCAACG	26875	0.0	29.819775	3
TATCAAC	27670	0.0	29.193117	2
CAACGCA	27475	0.0	29.128044	5
AACGCAG	28435	0.0	28.143614	6
TGGTATC	5125	0.0	26.089605	2
TACACAT	24250	0.0	25.5825	5
GTGGTAT	5715	0.0	25.327305	1
ACACATC	23750	0.0	25.101757	6
ACGCAGA	32230	0.0	24.79028	7
ACATCTC	24350	0.0	24.605213	8
CGCAGAG	32620	0.0	24.506899	8
CACATCT	26035	0.0	22.800877	7
ATACACA	30275	0.0	21.787748	4
CATCTCC	27570	0.0	21.500624	9
>>END_MODULE