FastQCFastQC Report
Fri 27 May 2016
SRR2139280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7920067
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107200.050.686721
GTATCAA193200.037.6613461
TCAACGC233950.030.5712764
ATCAACG235700.030.380763
TATCAAC242700.029.7511182
CAACGCA242250.029.5148935
AACGCAG248850.028.749096
ACGCAGA282950.025.269267
CGCAGAG287800.024.8731258
GTGGTAT51700.023.1531941
TGGTATC47700.023.123032
GCAGAGT338100.021.1979669
GAGTACT208050.020.52126112-13
GTACATG232850.020.434421
TACATGG230900.020.1256082
ACATGGG240600.018.9943963
CAGAGTA329200.018.96042410-11
AGAGTAC305650.018.61058210-11
GTACTTT231850.018.2955214-15
CTTATAC69550.018.2558781
Produced by FastQC (version 0.11.5)