##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139280_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7920067
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79314430042069	33.0	33.0	33.0	33.0	33.0
2	32.791346462094324	33.0	33.0	33.0	33.0	33.0
3	32.79289733281297	33.0	33.0	33.0	33.0	33.0
4	36.73232549674138	37.0	37.0	37.0	37.0	37.0
5	36.753125573306384	37.0	37.0	37.0	37.0	37.0
6	36.75806820826137	37.0	37.0	37.0	37.0	37.0
7	36.7435709824172	37.0	37.0	37.0	37.0	37.0
8	36.73887683020863	37.0	37.0	37.0	37.0	37.0
9	36.73580589659153	37.0	37.0	37.0	37.0	37.0
10-11	36.69053999164401	37.0	37.0	37.0	37.0	37.0
12-13	36.69085021629236	37.0	37.0	37.0	37.0	37.0
14-15	38.966522126643625	40.0	40.0	40.0	37.0	40.0
16-17	38.913897761218436	40.0	40.0	40.0	37.0	40.0
18-19	38.93685874122025	40.0	40.0	40.0	37.0	40.0
20-21	38.97750991294578	40.0	40.0	40.0	37.0	40.0
22-23	39.010164596010796	40.0	40.0	40.0	37.0	40.0
24-25	39.01720289493275	40.0	40.0	40.0	37.0	40.0
26-27	38.924898951199296	40.0	40.0	40.0	37.0	40.0
28-29	38.853878864981084	40.0	40.0	40.0	37.0	40.0
30-31	38.783151829488034	40.0	37.0	40.0	37.0	40.0
32-33	38.724795629486124	40.0	37.0	40.0	37.0	40.0
34-35	38.72020188973877	40.0	37.0	40.0	37.0	40.0
36-37	38.68421213950149	40.0	37.0	40.0	37.0	40.0
38-39	38.64211786066868	40.0	37.0	40.0	37.0	40.0
40-41	38.59612716605533	40.0	37.0	40.0	37.0	40.0
42-43	38.472764088672086	40.0	37.0	40.0	37.0	40.0
44-45	38.4329005976716	40.0	37.0	40.0	37.0	40.0
46-47	38.3681859391513	40.0	37.0	40.0	37.0	40.0
48-49	38.29667202113299	40.0	37.0	40.0	37.0	40.0
50-51	38.2461486677406	40.0	37.0	40.0	37.0	40.0
52-53	38.202415562389646	40.0	37.0	40.0	37.0	40.0
54-55	38.12606566455986	40.0	37.0	40.0	37.0	40.0
56-57	38.023668463376275	40.0	37.0	40.0	37.0	40.0
58-59	37.76591397249178	40.0	37.0	40.0	33.0	40.0
60-61	37.68925115631734	40.0	37.0	40.0	33.0	40.0
62-63	37.33845592665482	37.0	37.0	40.0	33.0	40.0
64-65	37.147772189925846	37.0	37.0	40.0	33.0	40.0
66-67	37.004730003354794	37.0	37.0	40.0	33.0	40.0
68-69	36.77980004255609	37.0	37.0	40.0	33.0	40.0
70-71	36.50741480485535	37.0	37.0	40.0	33.0	40.0
72-73	36.268493861512525	37.0	37.0	37.0	33.0	40.0
74-75	36.01918315143102	37.0	37.0	37.0	33.0	40.0
76-77	34.220846349999746	35.0	33.0	37.0	30.0	37.0
78-79	35.59854139374847	37.0	35.0	37.0	33.0	37.0
80-81	35.73968663135359	37.0	37.0	37.0	33.0	37.0
82-83	35.68225641322178	37.0	37.0	37.0	33.0	37.0
84-85	35.24696227955173	37.0	33.0	37.0	33.0	37.0
86-87	35.34412040624119	37.0	33.0	37.0	33.0	37.0
88-89	35.34919588106439	37.0	33.0	37.0	33.0	37.0
90-91	35.2959753838835	37.0	33.0	37.0	33.0	37.0
92-93	35.22394298756173	37.0	33.0	37.0	33.0	37.0
94-95	35.17524252178984	37.0	33.0	37.0	33.0	37.0
96-97	35.13983123326268	37.0	33.0	37.0	33.0	37.0
98-99	35.107497443534335	37.0	33.0	37.0	33.0	37.0
100	35.036067497567444	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	0.0
19	5.0
20	25.0
21	131.0
22	563.0
23	1624.0
24	3761.0
25	7989.0
26	14674.0
27	24033.0
28	36756.0
29	52798.0
30	72481.0
31	96840.0
32	131042.0
33	178994.0
34	254446.0
35	393731.0
36	761743.0
37	2243094.0
38	3498902.0
39	146434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94408961439341	19.98067693114212	14.339853438108541	23.735380016355922
2	14.497516245759032	20.754521394831634	42.12268658838366	22.625275771025674
3	18.17979567313307	25.924458933299764	30.435266252076875	25.46047914149029
4	12.348966637768495	18.01629210190748	38.906095594342425	30.728645665981595
5	13.395719855608796	37.51884657746406	35.52157970209876	13.563853864828387
6	29.898560201574053	37.771182996305456	18.78484108783423	13.545415714286255
7	26.421468404244557	32.744053806615526	22.8764605147911	17.958017274348816
8	24.33120830922264	36.67586397943351	21.14182872442872	17.851098986915137
9	25.383345873210416	17.134451009063433	21.268469067244002	36.21373405048215
10-11	24.070869602492	26.847392831399024	28.79169456521012	20.290043000898855
12-13	24.973589238575887	24.402419828014082	28.93834105191282	21.685649881497216
14-15	22.10867660589235	25.8124268393184	26.725701941662866	25.35319461312638
16-17	21.62090295448258	28.994016085975034	27.340892444470484	22.044188515071905
18-19	21.569419804150648	27.925515024052196	29.275415220603563	21.229649951193593
20-21	22.392148492175824	27.102147872850253	29.181440359357826	21.324263275616104
22-23	22.073245871925824	27.11314155515854	29.05873285786866	21.75487971504698
24-25	21.81519624460012	27.2516814073454	29.212226621327314	21.720895726727164
26-27	21.777652969656398	27.398195319219415	29.099597660210012	21.724554050914172
28-29	21.610010767081498	27.37124064585284	29.126897348636227	21.891851238429435
30-31	21.944152761346526	27.224162519088168	29.079184694075355	21.752500025489947
32-33	21.754909361893187	27.450947302065927	28.986937573400056	21.80720576264083
34-35	21.847292377874886	27.57777351884173	29.005572287039538	21.56936181624384
36-37	21.896705134338767	27.415878182653202	28.859358043175686	21.82805863983234
38-39	21.774780488832853	27.506048346682444	28.938486245440288	21.780684919044415
40-41	22.022738369976025	27.38682303835987	28.815266893814556	21.77517169784955
42-43	21.965079316292567	27.477771329651652	28.637112137833387	21.920037216222397
44-45	22.039659096818795	27.6551441468464	28.407895835839486	21.897300920495322
46-47	22.177206674922058	27.590049489261464	28.014107743926964	22.21863609188952
48-49	22.07049510661714	27.70116620084361	28.045125686611332	22.183213005927925
50-51	22.00597512396818	27.891171507553658	27.979892146234377	22.122961222243784
52-53	22.197388907566314	27.810858055605785	27.913332259994526	22.078420776833376
54-55	22.146079302130637	27.718807453215682	27.881802185512033	22.253311059141645
56-57	22.23012440214596	27.856656416034202	27.750707337736753	22.162511844083085
58-59	22.151434442182673	27.96117234397611	27.85400682439696	22.033386389444264
60-61	22.255494710166886	27.994874012515	27.728918949349012	22.020712327969093
62-63	22.189777095888182	28.092615263685726	27.64932977430634	22.068277866119747
64-65	22.14968290800891	28.06652216220302	27.63569216122006	22.14810276856801
66-67	22.208052108732907	28.01591365354039	27.540236446650756	22.235797791075946
68-69	22.200521791413777	27.96270022888272	27.599516644470462	22.23726133523304
70-71	22.178409778194474	27.700379341640772	27.673378583250024	22.447832296914733
72-73	22.252622241380823	27.721026075884435	27.608980712486066	22.417370970248673
74-75	22.102382948924866	27.89462303219504	27.612768698317375	22.39022532056272
76-77	22.245814191939704	27.82897148544814	27.50012299289101	22.42509132972114
78-79	22.25451484705617	27.886517039969462	27.435862467606835	22.423105645367528
80-81	22.2330332732399	27.877630741377917	27.42841070935183	22.460925276030352
82-83	22.276146949550796	27.863037132393494	27.541386254661038	22.319429663394672
84-85	22.421317062520256	27.798207353340924	27.427255954909825	22.35321962922899
86-87	22.21485384081785	27.825644792225766	27.59556139376191	22.363939973194473
88-89	22.24948243183214	27.85616991317782	27.534072049544594	22.360275605445445
90-91	22.265802767680334	27.861998875973022	27.577878726420764	22.294319629925884
92-93	22.28833136414207	27.816247017682898	27.586491849749656	22.308929768425372
94-95	22.139027872369574	27.961197552900213	27.61049869523408	22.28927587949613
96-97	22.236130299338008	27.990930733023205	27.70851672108577	22.06442224655302
98-99	23.34230814149324	29.904283242600744	27.882502911814953	18.870905704091058
100	22.31181547251582	30.937517817018325	21.76255101566916	24.988115694796694
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2262.0
1	1952.0
2	2260.0
3	3106.5
4	4240.0
5	6821.0
6	8960.5
7	10461.0
8	11612.5
9	11669.5
10	10883.5
11	10178.5
12	11198.0
13	14474.5
14	22217.0
15	32992.5
16	41991.0
17	47686.0
18	49347.0
19	47202.5
20	43149.0
21	38960.5
22	36690.5
23	38161.0
24	44055.5
25	54077.0
26	67661.5
27	83372.0
28	98855.0
29	115742.5
30	132263.0
31	147967.0
32	165797.5
33	183570.5
34	201892.5
35	218925.0
36	237085.0
37	254214.0
38	266254.0
39	276107.0
40	287366.5
41	297570.5
42	303081.5
43	309821.0
44	316310.5
45	323123.0
46	330570.0
47	333529.0
48	330539.0
49	323888.5
50	316688.5
51	305907.5
52	291282.5
53	275268.0
54	258305.0
55	239690.0
56	220866.5
57	201209.5
58	180768.0
59	159389.0
60	134089.0
61	108416.0
62	85015.5
63	65209.5
64	48786.0
65	35982.0
66	27112.5
67	20919.5
68	15920.5
69	11340.0
70	7945.0
71	5698.0
72	4038.5
73	2766.0
74	1824.5
75	1122.5
76	719.5
77	485.5
78	326.0
79	215.0
80	157.5
81	115.0
82	79.0
83	48.5
84	32.0
85	20.0
86	14.5
87	14.0
88	11.5
89	8.0
90	3.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.001388877139549451
4	0.032966892830578326
5	0.0024494742279326676
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.0505627621123704E-5
22-23	0.0
24-25	0.0035488522241515687
26-27	0.0410758577546141
28-29	0.08830033235860855
30-31	0.08612294871916554
32-33	0.03914194415093976
34-35	0.0025506319524597823
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.0513659293556074E-5
56-57	1.0335140352239498E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.9253250804062166E-5
72-73	0.011874365671017085
74-75	0.024912955249862437
76-77	0.003254705937318563
78-79	0.0
80-81	0.0
82-83	0.005176095067612742
84-85	0.04293317192201645
86-87	0.044198591684625206
88-89	0.011207176014203887
90-91	1.1205041372214186E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	86.0
20-21	420.0
22-23	1027.0
24-25	2022.0
26-27	3450.0
28-29	7027.0
30-31	17041.0
32-33	21615.0
34-35	29574.0
36-37	43390.0
38-39	49390.0
40-41	52383.0
42-43	60689.0
44-45	71009.0
46-47	69753.0
48-49	61716.0
50-51	53812.0
52-53	50314.0
54-55	54138.0
56-57	57213.0
58-59	58354.0
60-61	57670.0
62-63	58309.0
64-65	61262.0
66-67	66052.0
68-69	60600.0
70-71	58511.0
72-73	56114.0
74-75	56096.0
76-77	55839.0
78-79	59149.0
80-81	60132.0
82-83	58244.0
84-85	58065.0
86-87	60774.0
88-89	64630.0
90-91	68633.0
92-93	73190.0
94-95	87852.0
96-97	253972.0
98-99	483648.0
100-101	5296902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.22331799795377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69726723786678	76.44298729360162
2	7.280487629133521	12.409346247956291
3	1.7136567238349405	4.381305357441503
4	0.6209621640812885	2.116818238967888
5	0.2793682498985967	1.1904344599819963
6	0.14235722096357462	0.7279292826890215
7	0.0734535990463865	0.4381971600737074
8	0.050247101436736094	0.342577976337472
9	0.03208117944395708	0.24606581017515952
>10	0.10721226097458131	1.4047946455999685
>50	0.0023077193811949855	0.13460385086102522
>100	4.8115741040811154E-4	0.06522603257753366
>500	7.065403368441667E-5	0.036911002035525206
>1k	4.710249433758441E-5	0.06280264170128393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2626155814085917E-5	0.0
3	0.0	0.0	0.0	1.2626155814085917E-5	0.0
4	0.0	5.0504623256343666E-5	0.0	2.5252311628171833E-5	0.0
5	0.0	6.313077907042957E-5	0.0	6.313077907042957E-5	0.0
6	1.2626155814085917E-5	6.313077907042957E-5	0.0	6.313077907042957E-5	0.0
7	1.2626155814085917E-5	6.313077907042957E-5	0.0	6.313077907042957E-5	0.0
8	1.2626155814085917E-5	7.57569348845155E-5	0.0	7.57569348845155E-5	0.0
9	1.2626155814085917E-5	1.641400255831169E-4	0.0	1.0100924651268733E-4	1.2626155814085917E-5
10-11	2.5252311628171833E-5	1.641400255831169E-4	0.0	1.325746360479021E-4	1.2626155814085917E-5
12-13	2.5252311628171833E-5	1.7045310349015987E-4	0.0	1.5151386976903098E-4	2.5252311628171833E-5
14-15	2.5252311628171833E-5	3.0934081744510495E-4	0.0	1.641400255831169E-4	4.419154534930071E-5
16-17	5.0504623256343666E-5	3.345931290732768E-4	0.0	1.8939233721128874E-4	6.313077907042957E-5
18-19	5.0504623256343666E-5	3.914108302366634E-4	0.0	2.0201849302537464E-4	6.313077907042957E-5
20-21	5.0504623256343666E-5	4.4822853140005E-4	0.0	2.272708046535465E-4	6.313077907042957E-5
22-23	5.0504623256343666E-5	4.924200767493507E-4	0.0	2.4621003837467536E-4	6.313077907042957E-5
24-25	5.0504623256343666E-5	5.492377779127374E-4	0.0	2.904015837239761E-4	6.944385697747254E-5
26-27	5.0504623256343666E-5	5.681770116338662E-4	0.0	3.7878467442257753E-4	8.207001279155845E-5
28-29	5.0504623256343666E-5	5.808031674479522E-4	0.0	7.386301151240261E-4	8.838309069860142E-5
30-31	5.0504623256343666E-5	6.313077907042958E-4	0.0	0.0018686710604847157	8.838309069860142E-5
32-33	5.0504623256343666E-5	6.313077907042958E-4	0.0	0.004084561405856794	8.838309069860142E-5
34-35	5.0504623256343666E-5	7.070647255888113E-4	0.0	0.007310544216355745	8.838309069860142E-5
36-37	5.0504623256343666E-5	7.891347383803698E-4	0.0	0.012266310373384468	1.1363540232677325E-4
38-39	5.0504623256343666E-5	8.270132058226275E-4	0.0	0.025700540159571884	1.1363540232677325E-4
40-41	5.0504623256343666E-5	0.0010353447767550452	0.0	0.04073197865624117	1.1363540232677325E-4
42-43	5.0504623256343666E-5	0.0010542840104761741	0.0	0.049677610050521036	1.2626155814085918E-4
44-45	5.0504623256343666E-5	0.001205797880245205	0.0	0.06009418859714191	1.4520079186198804E-4
46-47	5.0504623256343666E-5	0.0013383725162931072	0.0	0.0710410656879544	1.51513869769031E-4
48-49	5.0504623256343666E-5	0.0013383725162931072	0.0	0.08107254648224567	1.51513869769031E-4
50-51	5.0504623256343666E-5	0.0014393817628057944	0.0	0.09092726109513972	1.51513869769031E-4
52-53	5.0504623256343666E-5	0.0014520079186198804	0.0	0.11424145780584938	1.641400255831169E-4
54-55	5.0504623256343666E-5	0.0014646340744339662	0.0	0.14015032953635367	1.7676618139720284E-4
56-57	5.0504623256343666E-5	0.0014772602302480522	0.0	0.16790893309362157	1.7676618139720284E-4
58-59	5.0504623256343666E-5	0.0014898863860621381	0.0	0.19367891711017093	1.7676618139720284E-4
60-61	6.944385697747254E-5	0.0015340779314114389	0.0	0.20506139657656935	2.0201849302537467E-4
62-63	1.0100924651268733E-4	0.0015530171651325676	0.0	0.21800320628600744	2.0201849302537467E-4
64-65	1.0100924651268733E-4	0.0017676618139720282	0.0	0.22385542950583626	2.0201849302537467E-4
66-67	1.0100924651268733E-4	0.0017929141256002	0.0	0.2287859433512368	2.0201849302537467E-4
68-69	1.0100924651268733E-4	0.001824479515135415	0.0	0.23353337793733311	2.0201849302537467E-4
70-71	1.0100924651268733E-4	0.001830792593042458	0.0	0.23800935017342656	2.0201849302537467E-4
72-73	1.1363540232677325E-4	0.0018497318267635867	0.0	0.24115326297113396	2.1464464883946057E-4
74-75	1.1363540232677325E-4	0.0018749841383917587	0.0	0.24394364340604693	2.1464464883946057E-4
76-77	1.1363540232677325E-4	0.00196336722909036	0.0	0.24686659847700781	2.1464464883946057E-4
78-79	1.1363540232677325E-4	0.0020201849302537464	0.0	0.24857744258981646	2.1464464883946057E-4
80-81	1.1363540232677325E-4	0.0020201849302537464	0.0	0.24920243730261374	2.2095772674650353E-4
82-83	1.1363540232677325E-4	0.0020264980081607896	0.0	0.2497074835351772	2.398969604676324E-4
84-85	1.199484802338162E-4	0.0020454372418819184	0.0	0.2498716235607603	2.398969604676324E-4
86-87	1.325746360479021E-4	0.0020706895535100903	0.0	0.2499473804956448	2.4621003837467536E-4
88	1.641400255831169E-4	0.0021716988000227777	0.0	0.24996000665145887	2.525231162817183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10720	0.0	50.68672	1
GTATCAA	19320	0.0	37.661346	1
TCAACGC	23395	0.0	30.571276	4
ATCAACG	23570	0.0	30.38076	3
TATCAAC	24270	0.0	29.751118	2
CAACGCA	24225	0.0	29.514893	5
AACGCAG	24885	0.0	28.74909	6
ACGCAGA	28295	0.0	25.26926	7
CGCAGAG	28780	0.0	24.873125	8
GTGGTAT	5170	0.0	23.153194	1
TGGTATC	4770	0.0	23.12303	2
GCAGAGT	33810	0.0	21.197966	9
GAGTACT	20805	0.0	20.521261	12-13
GTACATG	23285	0.0	20.43442	1
TACATGG	23090	0.0	20.125608	2
ACATGGG	24060	0.0	18.994396	3
CAGAGTA	32920	0.0	18.960424	10-11
AGAGTAC	30565	0.0	18.610582	10-11
GTACTTT	23185	0.0	18.29552	14-15
CTTATAC	6955	0.0	18.255878	1
>>END_MODULE