FastQCFastQC Report
Fri 27 May 2016
SRR2139279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8498782
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117650.051.2784771
GTATCAA215150.037.459881
TCAACGC256750.030.8594364
ATCAACG259950.030.4471573
CAACGCA262450.030.1940675
TATCAAC268750.029.5769252
AACGCAG269450.029.4252826
ACGCAGA311250.025.4598777
CGCAGAG314000.025.250458
GTGGTAT56400.022.948361
GCAGAGT367300.021.6210429
TGGTATC55850.020.887412
GTACATG252350.020.4651371
GAGTACT224250.020.40149312-13
TACATGG252750.020.0284732
CAGAGTA358050.019.3863610-11
ACATGGG254750.019.269583
AGAGTAC331000.018.97130210-11
GTACTTT252100.018.04688614-15
CATGGGG185300.017.5078014
Produced by FastQC (version 0.11.5)