##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139279_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8498782
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.789415236206786	33.0	33.0	33.0	33.0	33.0
2	32.788695956667674	33.0	33.0	33.0	33.0	33.0
3	32.78888363061907	33.0	33.0	33.0	33.0	33.0
4	36.7277716971679	37.0	37.0	37.0	37.0	37.0
5	36.749514930492396	37.0	37.0	37.0	37.0	37.0
6	36.75670478428556	37.0	37.0	37.0	37.0	37.0
7	36.740915815936916	37.0	37.0	37.0	37.0	37.0
8	36.73631374472248	37.0	37.0	37.0	37.0	37.0
9	36.73442935705375	37.0	37.0	37.0	37.0	37.0
10-11	36.68848630309614	37.0	37.0	37.0	37.0	37.0
12-13	36.68847706647847	37.0	37.0	37.0	37.0	37.0
14-15	38.95938300335271	40.0	40.0	40.0	37.0	40.0
16-17	38.906177085139966	40.0	40.0	40.0	37.0	40.0
18-19	38.92764898546639	40.0	40.0	40.0	37.0	40.0
20-21	38.96773839364131	40.0	40.0	40.0	37.0	40.0
22-23	39.000937089305864	40.0	40.0	40.0	37.0	40.0
24-25	39.01096075313505	40.0	40.0	40.0	37.0	40.0
26-27	38.916995444242716	40.0	40.0	40.0	37.0	40.0
28-29	38.84413053091876	40.0	40.0	40.0	37.0	40.0
30-31	38.77100624138696	40.0	37.0	40.0	37.0	40.0
32-33	38.71492242609322	40.0	37.0	40.0	37.0	40.0
34-35	38.70826625891648	40.0	37.0	40.0	37.0	40.0
36-37	38.67175931125486	40.0	37.0	40.0	37.0	40.0
38-39	38.63347222009505	40.0	37.0	40.0	37.0	40.0
40-41	38.58475301267964	40.0	37.0	40.0	37.0	40.0
42-43	38.46176779808095	40.0	37.0	40.0	37.0	40.0
44-45	38.41995888348276	40.0	37.0	40.0	37.0	40.0
46-47	38.35232796858688	40.0	37.0	40.0	37.0	40.0
48-49	38.2796387148303	40.0	37.0	40.0	37.0	40.0
50-51	38.233233779587295	40.0	37.0	40.0	37.0	40.0
52-53	38.192055546716816	40.0	37.0	40.0	37.0	40.0
54-55	38.11745933629399	40.0	37.0	40.0	37.0	40.0
56-57	38.02044952660041	40.0	37.0	40.0	37.0	40.0
58-59	37.76895663311657	40.0	37.0	40.0	33.0	40.0
60-61	37.69130648250363	40.0	37.0	40.0	33.0	40.0
62-63	37.345985326341356	37.0	37.0	40.0	33.0	40.0
64-65	37.15192283771899	37.0	37.0	40.0	33.0	40.0
66-67	37.0010675737266	37.0	37.0	40.0	33.0	40.0
68-69	36.7748811492043	37.0	37.0	40.0	33.0	40.0
70-71	36.503156037242306	37.0	37.0	40.0	33.0	40.0
72-73	36.2647600843595	37.0	37.0	37.0	33.0	40.0
74-75	36.01738583180154	37.0	37.0	37.0	33.0	40.0
76-77	34.20993834023602	35.0	33.0	37.0	30.0	37.0
78-79	35.59713753569187	37.0	35.0	37.0	33.0	37.0
80-81	35.73972432320117	37.0	37.0	37.0	33.0	37.0
82-83	35.684884007702976	37.0	37.0	37.0	33.0	37.0
84-85	35.26023983418908	37.0	33.0	37.0	33.0	37.0
86-87	35.34877499320777	37.0	33.0	37.0	33.0	37.0
88-89	35.35023612565078	37.0	33.0	37.0	33.0	37.0
90-91	35.291512460082075	37.0	33.0	37.0	33.0	37.0
92-93	35.21792089773916	37.0	33.0	37.0	33.0	37.0
94-95	35.16992405077506	37.0	33.0	37.0	33.0	37.0
96-97	35.13280448988657	37.0	33.0	37.0	33.0	37.0
98-99	35.09598841100127	37.0	33.0	37.0	33.0	37.0
100	35.02724770020469	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	6.0
20	33.0
21	147.0
22	548.0
23	1670.0
24	3948.0
25	8280.0
26	15400.0
27	25581.0
28	39048.0
29	56245.0
30	77370.0
31	105426.0
32	141878.0
33	193849.0
34	277616.0
35	425593.0
36	822896.0
37	2404484.0
38	3740473.0
39	158287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11337577549348	19.907534985601465	14.291247851750994	23.687841387154066
2	14.537247807980014	20.857447573075767	42.04154195271746	22.56376266622676
3	18.209349736511964	25.91554827703791	30.460937571702328	25.4141644147478
4	12.376771363828325	18.03869728752226	38.87761952806946	30.70691182057995
5	13.385548447554827	37.566571564291884	35.502375049140674	13.545504939012618
6	29.949515118754665	37.80641743722806	18.763559295908518	13.480508148108752
7	26.464862847405662	32.83098684023193	22.872383360345047	17.831766952017357
8	24.28010272530817	36.82297063273302	21.09718780879425	17.799738833164565
9	25.497853692446753	16.990340498203153	21.220864354445144	36.29094145490495
10-11	24.110190142540425	26.83092118376492	28.843944932344424	20.21494374135023
12-13	25.032922364640015	24.42890051774478	28.956908178136587	21.58126893947862
14-15	22.135371868580698	25.857922935310025	26.77108908076475	25.235616115344527
16-17	21.59072911859605	29.003544272579298	27.373322436085544	22.032404172739106
18-19	21.569849656103663	27.979232788886687	29.331573630197834	21.119343924811815
20-21	22.40659091232673	27.14700084519694	29.241896116906634	21.204512125569696
22-23	22.096606631595527	27.108367844639375	29.10459392469611	21.690431599068987
24-25	21.838035722808293	27.22544593190253	29.21885803369488	21.717660311594297
26-27	21.825286291717394	27.40191363271627	29.101384797904213	21.671415277662128
28-29	21.666356783418962	27.467895840085628	29.1680053347865	21.69774204170891
30-31	22.082950647044797	27.28227019344054	29.024439192427653	21.61033996708701
32-33	21.838168854867167	27.43756451669565	28.976035244937815	21.748231383499363
34-35	21.893796887133778	27.557705120220017	28.99760056962897	21.550897423017236
36-37	21.942763648875466	27.44656229716476	28.92656852101957	21.684105532940205
38-39	21.75606893920738	27.562093314953252	28.957629823302337	21.724207922537026
40-41	22.03750492635235	27.437113063657254	28.786859663983684	21.738522346006707
42-43	21.93858961249466	27.5349862963853	28.62048819309789	21.905935898022147
44-45	22.064765928094925	27.71600962928764	28.358352705234285	21.860871737383153
46-47	22.156602724678674	27.652691578736828	28.037907419813514	22.152798276770984
48-49	22.098316571438723	27.770948031475573	28.020140294261914	22.110595102823787
50-51	22.076546721711455	27.912067616073198	27.94048446276579	22.07090119944955
52-53	22.22037545985442	27.836256232028493	27.850163094187845	22.093205213929235
54-55	22.22291390593601	27.721773812636847	27.865150880234772	22.190161401192373
56-57	22.199083005116407	27.9146454111028	27.769479845452043	22.116791738328754
58-59	22.2061094953886	27.968042751711646	27.76932418561106	22.056523567288693
60-61	22.263409155100643	27.959829683236535	27.75594024663083	22.020820915031994
62-63	22.230482710161624	28.045382126066848	27.671826057960814	22.052309105810714
64-65	22.234859649889994	27.962040092973666	27.669893416768844	22.1332068403675
66-67	22.30497092562356	27.99454223163132	27.534795698067242	22.165691144677872
68-69	22.219176367088426	27.95075448127573	27.641078696732585	22.18899045490326
70-71	22.253187836704193	27.69167209380286	27.700937545778604	22.354202523714346
72-73	22.241745869821397	27.77367639724771	27.637788857885987	22.346788875044904
74-75	22.1196996673881	27.939043553588366	27.589703707358478	22.35155307166505
76-77	22.302017169218367	27.89027514888509	27.49982828529638	22.30787939660016
78-79	22.262506433807218	27.905109774062435	27.514793319489556	22.317590472640788
80-81	22.25972171209955	27.93037829837598	27.490420481361227	22.319479508163244
82-83	22.306256847255327	27.87066885335251	27.593972477035024	22.229101822357137
84-85	22.40000747640406	27.818633535293593	27.4648550599777	22.31650392832465
86-87	22.214348758149587	27.87638155342439	27.598294732969443	22.310974955456583
88-89	22.287667079035245	27.862724051009685	27.546940256164596	22.302668613790473
90-91	22.268476784314586	27.913567353623502	27.568868038193695	22.249087823868216
92-93	22.313943978736457	27.87539948252865	27.608327309148972	22.202329229585928
94-95	22.203143594506898	27.950883511245088	27.578398503129442	22.26757439111858
96-97	22.299076308771586	28.019287094219514	27.660848057777887	22.020788539231013
98-99	23.34863264032982	29.979860856829	27.87176044590911	18.799746056932072
100	22.27704838520254	31.001244321695655	21.768391532367797	24.953315760734004
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2472.0
1	2113.5
2	2496.0
3	3482.5
4	4716.0
5	7479.0
6	9860.0
7	11709.0
8	13161.0
9	13232.0
10	12282.0
11	11359.5
12	12312.5
13	15817.5
14	24275.0
15	36085.5
16	45766.5
17	51784.5
18	53543.5
19	51147.5
20	46997.5
21	42835.0
22	40277.0
23	41435.0
24	47618.5
25	58571.5
26	73014.0
27	89687.0
28	106722.5
29	125421.5
30	144079.5
31	162516.0
32	182437.0
33	200845.5
34	219463.0
35	236545.5
36	254776.5
37	272638.0
38	284963.5
39	294977.5
40	307412.5
41	319074.0
42	326953.0
43	334915.0
44	341536.0
45	348523.0
46	354672.5
47	356072.0
48	352333.5
49	346512.5
50	339884.0
51	328604.5
52	312736.5
53	295312.0
54	278019.5
55	259224.0
56	239185.5
57	217712.0
58	193969.5
59	169575.0
60	143424.5
61	117936.5
62	94657.0
63	73768.5
64	55910.0
65	41650.5
66	31137.5
67	23768.5
68	18216.5
69	13196.0
70	9431.0
71	6890.0
72	4991.0
73	3471.5
74	2337.0
75	1567.0
76	1063.5
77	667.5
78	426.5
79	282.0
80	189.0
81	142.5
82	110.0
83	78.0
84	55.0
85	38.5
86	27.0
87	19.5
88	12.5
89	6.5
90	4.0
91	4.0
92	3.5
93	3.5
94	3.0
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012237047614587597
4	0.03278116793677024
5	0.0026003726180998644
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	5.2949801058772454E-5
22-23	0.0
24-25	0.0032424688805373246
26-27	0.04078164665787695
28-29	0.08796515884705389
30-31	0.08668145209183728
32-33	0.03921360667812511
34-35	0.002164647603969359
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.2808956022050617E-5
56-57	1.0977322659415262E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	1.3716433315844881E-5
72-73	0.01167776884880444
74-75	0.024761279359279395
76-77	0.0034130651147377487
78-79	0.0
80-81	0.0
82-83	0.004917776227874707
84-85	0.04112520607304348
86-87	0.04260909076443538
88-89	0.010453845713142009
90-91	1.1281383964084285E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	97.0
20-21	406.0
22-23	1050.0
24-25	2411.0
26-27	4034.0
28-29	7994.0
30-31	19722.0
32-33	24625.0
34-35	33569.0
36-37	49398.0
38-39	57858.0
40-41	59048.0
42-43	68411.0
44-45	80512.0
46-47	78161.0
48-49	69132.0
50-51	61273.0
52-53	58683.0
54-55	62971.0
56-57	65097.0
58-59	64347.0
60-61	63463.0
62-63	61572.0
64-65	64235.0
66-67	68055.0
68-69	65943.0
70-71	63962.0
72-73	61208.0
74-75	61342.0
76-77	61393.0
78-79	64294.0
80-81	65101.0
82-83	62773.0
84-85	63175.0
86-87	66086.0
88-89	70769.0
90-91	74126.0
92-93	77898.0
94-95	94616.0
96-97	270341.0
98-99	512825.0
100-101	5636806.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.00801455929302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.63579370259967	76.19760856104378
2	7.266456227240045	12.354140335193744
3	1.720871526548709	4.388636153505764
4	0.6352905883461799	2.160191663340555
5	0.29232604082324476	1.2425028167181436
6	0.14551363867234446	0.7421895308900617
7	0.09218056892861588	0.5485261001897385
8	0.05677620521952266	0.38611459839380724
9	0.03624823059340813	0.27732511026297435
>10	0.11592345001713916	1.500002283141644
>50	0.002257087753256375	0.12993768514059723
>100	3.1811947952915256E-4	0.04022849595724641
>500	2.2307120310458466E-5	0.01060022760543191
>1k	2.2306658003301432E-5	0.02199643861644029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.529917581131038E-5	0.0	3.529917581131038E-5	0.0
3	0.0	3.529917581131038E-5	0.0	3.529917581131038E-5	0.0
4	0.0	8.236474355972421E-5	0.0	3.529917581131038E-5	1.1766391937103458E-5
5	0.0	8.236474355972421E-5	0.0	3.529917581131038E-5	1.1766391937103458E-5
6	9.413113549682766E-5	8.236474355972421E-5	0.0	3.529917581131038E-5	2.3532783874206916E-5
7	9.413113549682766E-5	8.236474355972421E-5	0.0	8.236474355972421E-5	3.529917581131038E-5
8	9.413113549682766E-5	9.413113549682766E-5	0.0	8.236474355972421E-5	3.529917581131038E-5
9	9.413113549682766E-5	2.5886062261627605E-4	0.0	9.413113549682766E-5	3.529917581131038E-5
10-11	9.413113549682766E-5	2.5886062261627605E-4	0.0	1.0589752743393112E-4	4.1182371779862104E-5
12-13	9.413113549682766E-5	2.5886062261627605E-4	0.0	1.0589752743393112E-4	4.706556774841383E-5
14-15	9.413113549682766E-5	3.706413460187589E-4	0.0	1.1766391937103459E-4	5.883195968551729E-5
16-17	1.5296309518234496E-4	3.882909339244141E-4	0.0	1.3531350727668978E-4	5.883195968551729E-5
18-19	1.5296309518234496E-4	4.059405218300693E-4	0.0	1.7061268308800014E-4	8.824793952827594E-5
20-21	1.5296309518234496E-4	4.2359010973572447E-4	0.0	1.7649587905655187E-4	1.411967032452415E-4
22-23	1.5296309518234496E-4	4.7653887345269004E-4	0.0	1.9414546696220705E-4	1.588462911508967E-4
24-25	1.5296309518234496E-4	5.177212452325521E-4	0.0	2.4121103471062087E-4	2.1179505486786223E-4
26-27	1.5296309518234496E-4	5.41254029106759E-4	0.0	3.2945897423889683E-4	2.1179505486786223E-4
28-29	1.5296309518234496E-4	5.41254029106759E-4	0.0	5.706700089495177E-4	2.1179505486786223E-4
30-31	1.5296309518234496E-4	6.236187726664832E-4	0.0	0.0014237334243895183	2.1767825083641396E-4
32-33	1.5296309518234496E-4	6.236187726664832E-4	0.0	0.00312986025526952	2.235614468049657E-4
34-35	1.6472948711944842E-4	7.236331041318626E-4	0.0	0.004941884613583452	2.7651021052193126E-4
36-37	1.6472948711944842E-4	8.295306315657938E-4	0.0	0.007918781773670627	2.82393406490483E-4
38-39	1.7649587905655187E-4	8.471802194714489E-4	0.0	0.012189982046839182	3.059261903646899E-4
40-41	1.823790750251036E-4	9.413113549682767E-4	0.0	0.016884772429743462	3.059261903646899E-4
42-43	2.0002866293075878E-4	9.58960942873932E-4	0.0	0.021103023939195054	3.059261903646899E-4
44-45	2.0002866293075878E-4	0.001041325686433656	0.0	0.026162572472149537	3.059261903646899E-4
46-47	2.0002866293075878E-4	0.001106040842087725	0.0	0.031792791014053544	3.235757782703451E-4
48-49	2.059118588993105E-4	0.0011178072340248286	0.0	0.03734064481239782	3.412253661760003E-4
50-51	2.3532783874206917E-4	0.0013413686808297944	0.0	0.042706119535717	3.412253661760003E-4
52-53	2.3532783874206917E-4	0.0013766678566411047	0.0	0.04814807580662735	3.47108562144552E-4
54-55	2.3532783874206917E-4	0.0013766678566411047	0.0	0.05366651362512887	3.647581500502072E-4
56-57	2.3532783874206917E-4	0.0014060838364838633	0.0	0.059367330518655496	3.7652454198731065E-4
58-59	2.3532783874206917E-4	0.0014178502284209666	0.0	0.06487988514118848	4.0005732586151756E-4
60-61	2.5886062261627605E-4	0.0014707989921379323	0.0	0.07017476151288501	4.412396976413796E-4
62-63	2.9415979842758647E-4	0.001494331776012139	0.0	0.07559906819588971	4.471228936099314E-4
64-65	2.9415979842758647E-4	0.0017061268308800014	0.0	0.08058801837722158	4.471228936099314E-4
66-67	3.059261903646899E-4	0.0017296596147542084	0.0	0.08645356475786767	4.588892855470349E-4
68-69	3.059261903646899E-4	0.001741426006691312	0.0	0.09209554969170877	4.7065567748413834E-4
70-71	3.118093863332417E-4	0.0017649587905655187	0.0	0.0966491433713678	4.824220694212418E-4
72-73	3.412253661760003E-4	0.0017826083784711739	0.0	0.10040262239930381	5.41254029106759E-4
74-75	3.412253661760003E-4	0.0018237907502510358	0.0	0.10343835151907649	5.41254029106759E-4
76-77	3.412253661760003E-4	0.0018943891018736569	0.0	0.10647996383481775	5.41254029106759E-4
78-79	3.412253661760003E-4	0.0019120386897793118	0.0	0.10835082015281719	5.471372250753108E-4
80-81	3.412253661760003E-4	0.0019179218857478637	0.0	0.10894502294564092	5.589036170124143E-4
82-83	3.47108562144552E-4	0.0019179218857478637	0.0	0.10936272985940809	5.64786812980966E-4
84-85	3.588749540816555E-4	0.0019414546696220705	0.0	0.10955099213040174	5.64786812980966E-4
86-87	3.824077379558624E-4	0.001953221061559174	0.0	0.10960982409008727	5.706700089495177E-4
88	4.0005732586151756E-4	0.0020708849809302084	0.0	0.10961570728605581	5.765532049180694E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11765	0.0	51.278477	1
GTATCAA	21515	0.0	37.45988	1
TCAACGC	25675	0.0	30.859436	4
ATCAACG	25995	0.0	30.447157	3
CAACGCA	26245	0.0	30.194067	5
TATCAAC	26875	0.0	29.576925	2
AACGCAG	26945	0.0	29.425282	6
ACGCAGA	31125	0.0	25.459877	7
CGCAGAG	31400	0.0	25.25045	8
GTGGTAT	5640	0.0	22.94836	1
GCAGAGT	36730	0.0	21.621042	9
TGGTATC	5585	0.0	20.88741	2
GTACATG	25235	0.0	20.465137	1
GAGTACT	22425	0.0	20.401493	12-13
TACATGG	25275	0.0	20.028473	2
CAGAGTA	35805	0.0	19.38636	10-11
ACATGGG	25475	0.0	19.26958	3
AGAGTAC	33100	0.0	18.971302	10-11
GTACTTT	25210	0.0	18.046886	14-15
CATGGGG	18530	0.0	17.507801	4
>>END_MODULE