FastQCFastQC Report
Fri 27 May 2016
SRR2139278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8108988
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC98600.051.880811
GGTATCA107850.051.6568951
TATACAC133600.040.4572073
GTATCAA194550.038.5101551
TTATACA140200.037.5197262
TCAACGC238900.030.5763764
ATCAACG239000.030.545953
CAACGCA243100.030.0224445
TATCAAC248100.029.7860852
TACACAT185100.029.4228215
AACGCAG249200.029.3895246
ACACATC187750.027.8049856
ACATCTC197800.026.413778
ACGCAGA288650.025.2843277
CACATCT206300.025.0984277
CGCAGAG292550.024.9618158
ATACACA243500.023.8415724
GTGGTAT50100.023.6345731
TGGTATC49100.023.5086942
CATCTCC228150.022.6574159
Produced by FastQC (version 0.11.5)