##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139278_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8108988
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78779102891754	33.0	33.0	33.0	33.0	33.0
2	32.793610744028726	33.0	33.0	33.0	33.0	33.0
3	32.795198365073425	33.0	33.0	33.0	33.0	33.0
4	36.73231826215552	37.0	37.0	37.0	37.0	37.0
5	36.752326554189004	37.0	37.0	37.0	37.0	37.0
6	36.7568824864459	37.0	37.0	37.0	37.0	37.0
7	36.7421066599186	37.0	37.0	37.0	37.0	37.0
8	36.73740631999949	37.0	37.0	37.0	37.0	37.0
9	36.73714155206544	37.0	37.0	37.0	37.0	37.0
10-11	36.69157069908107	37.0	37.0	37.0	37.0	37.0
12-13	36.69080926004577	37.0	37.0	37.0	37.0	37.0
14-15	38.96153909711052	40.0	40.0	40.0	37.0	40.0
16-17	38.903899352175635	40.0	40.0	40.0	37.0	40.0
18-19	38.926687152576875	40.0	40.0	40.0	37.0	40.0
20-21	38.96107841561664	40.0	40.0	40.0	37.0	40.0
22-23	38.992068988152866	40.0	40.0	40.0	37.0	40.0
24-25	39.0036233066066	40.0	40.0	40.0	37.0	40.0
26-27	38.900132506531186	40.0	40.0	40.0	37.0	40.0
28-29	38.82299023126774	40.0	40.0	40.0	37.0	40.0
30-31	38.7483920558068	40.0	37.0	40.0	37.0	40.0
32-33	38.69853849255115	40.0	37.0	40.0	37.0	40.0
34-35	38.69540197471841	40.0	37.0	40.0	37.0	40.0
36-37	38.649669208189934	40.0	37.0	40.0	37.0	40.0
38-39	38.591615352782384	40.0	37.0	40.0	37.0	40.0
40-41	38.553944272896665	40.0	37.0	40.0	37.0	40.0
42-43	38.439802092753546	40.0	37.0	40.0	37.0	40.0
44-45	38.40027922608604	40.0	37.0	40.0	37.0	40.0
46-47	38.334913900098414	40.0	37.0	40.0	37.0	40.0
48-49	38.26092262888878	40.0	37.0	40.0	37.0	40.0
50-51	38.17292875540919	40.0	37.0	40.0	37.0	40.0
52-53	38.141423932544875	40.0	37.0	40.0	37.0	40.0
54-55	38.072447268885945	40.0	37.0	40.0	37.0	40.0
56-57	37.968344153150724	40.0	37.0	40.0	33.0	40.0
58-59	37.724635684550634	40.0	37.0	40.0	33.0	40.0
60-61	37.633185394202954	40.0	37.0	40.0	33.0	40.0
62-63	37.29888826219201	37.0	37.0	40.0	33.0	40.0
64-65	37.12111790103009	37.0	37.0	40.0	33.0	40.0
66-67	36.984574048058235	37.0	37.0	40.0	33.0	40.0
68-69	36.76580347352211	37.0	37.0	40.0	33.0	40.0
70-71	36.50003565537591	37.0	37.0	40.0	33.0	40.0
72-73	36.26610710631212	37.0	37.0	37.0	33.0	40.0
74-75	36.019563618237186	37.0	37.0	37.0	33.0	40.0
76-77	34.227489537469914	35.0	33.0	37.0	30.0	37.0
78-79	35.60245632184397	37.0	35.0	37.0	33.0	37.0
80-81	35.74314132061071	37.0	37.0	37.0	33.0	37.0
82-83	35.68845104946314	37.0	37.0	37.0	33.0	37.0
84-85	35.2656176177896	37.0	33.0	37.0	33.0	37.0
86-87	35.35749821371098	37.0	33.0	37.0	33.0	37.0
88-89	35.36204083737962	37.0	33.0	37.0	33.0	37.0
90-91	35.30837049076794	37.0	33.0	37.0	33.0	37.0
92-93	35.23886839099486	37.0	33.0	37.0	33.0	37.0
94-95	35.19108551724028	37.0	33.0	37.0	33.0	37.0
96-97	35.15875997655789	37.0	33.0	37.0	33.0	37.0
98-99	35.12583718054505	37.0	33.0	37.0	33.0	37.0
100	35.0542591550272	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	3.0
20	32.0
21	151.0
22	632.0
23	1864.0
24	4488.0
25	9155.0
26	16239.0
27	26184.0
28	39107.0
29	55268.0
30	75556.0
31	100862.0
32	134816.0
33	184397.0
34	264211.0
35	405821.0
36	786147.0
37	2311383.0
38	3549629.0
39	143041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.132740608322514	20.121573740151053	14.52453746386109	24.221148187665342
2	14.27895811413212	20.521611328072012	42.34782441409458	22.851606143701286
3	17.91187516209076	25.866731583129265	30.91219838786674	25.309194866913238
4	12.176003420550716	18.319141279921027	38.17543324652428	31.32942205300398
5	13.272905082015935	37.45413310740567	35.52613891838363	13.746822892194766
6	29.811537518615143	37.626162475514825	18.575499186828246	13.986800819041784
7	26.164091006177344	32.85620844425963	22.330480696234844	18.649219853328184
8	24.318718932621433	36.58126020164292	21.194272330899985	17.905748534835666
9	25.005364417853375	16.969133509631533	20.80839187331391	37.21711019920118
10-11	23.92247343318303	26.67450611592963	28.68771047632578	20.71530997456156
12-13	24.891453779435853	24.13696998935009	28.645109599372944	22.326466631841114
14-15	22.176620559803517	25.704113756241842	26.17161352316713	25.94765216078751
16-17	21.880948646119588	29.11157964471029	26.77194737493754	22.235524334232583
18-19	21.993971380892415	27.697963050383105	28.613743169924533	21.694322398799947
20-21	22.60761197079777	26.985553901529357	28.566009436869642	21.840824690803228
22-23	22.05545063600651	27.24351096261634	28.612541498994432	22.088496902382722
24-25	22.156750203009313	27.229207535281308	28.653140689431094	21.960901572278285
26-27	22.172283414473952	27.503642702889557	28.65374255204657	21.670331330589924
28-29	21.788053314743895	27.122091494966288	28.85271218505604	22.23714300523378
30-31	22.04071028111135	27.08787057353726	28.99143191760204	21.879987227749353
32-33	21.55617888819392	27.03511254460686	29.291685170585325	22.117023396613895
34-35	21.630865105689544	27.24098860918322	28.95241793891257	22.175728346214658
36-37	21.795439430260444	27.314866189556252	28.970829500315826	21.918864879867474
38-39	21.821015883244954	27.135348917974706	28.896084072058542	22.147551126721805
40-41	21.94757891436939	27.023732893226736	28.97556317170686	22.053125020697014
42-43	22.159075108748777	26.98835632241268	28.963192458002347	21.8893761108362
44-45	21.90788814369041	27.417919909444755	28.594240900350957	22.079951046513877
46-47	22.485731169900383	27.012674643551275	27.870673961227943	22.630920225320395
48-49	22.191655550460627	27.135184355329528	28.177172349827007	22.49598774438284
50-51	21.975958816550182	27.527729412787938	28.180317081055712	22.315994689606168
52-53	22.091410659409664	27.653450823555577	28.366256099166055	21.888882417868707
54-55	22.001006349350526	27.71090017789582	27.91958847823223	22.368504994521416
56-57	22.43737120486007	27.984308208815822	27.42466587120113	22.15365471512298
58-59	21.957079755825887	28.296277536382192	27.99340576445638	21.75323694333554
60-61	22.343020327314374	28.60125965251561	27.29060611674886	21.765113903421156
62-63	22.023405377495084	28.858096977206543	27.244273585196428	21.874224060101945
64-65	22.063230516269712	28.58675633885698	27.34584529236754	22.00416785250577
66-67	22.210564231780154	28.323390466382126	27.30552958112145	22.160515720716273
68-69	22.261069294219578	27.997613340682708	27.515011093261922	22.226306271835792
70-71	22.268633385860394	27.70525264550025	27.612201957190752	22.413912011448605
72-73	22.30997495953995	27.737529156887497	27.55317567517766	22.399320208394894
74-75	22.18559551385972	27.838569284991966	27.526417028734002	22.449418172414312
76-77	22.321220059161387	27.81946010173587	27.44544272887252	22.413877110230224
78-79	22.270215618809456	27.87534745565617	27.42752319923184	22.426913726302534
80-81	22.268952134345543	27.849003106944377	27.43203276249455	22.450011996215533
82-83	22.34206618128315	27.81062079507467	27.496354598915286	22.350958424726894
84-85	22.45648696007046	27.755300880555563	27.415058993596354	22.37315316577763
86-87	22.255579393918534	27.784542229791793	27.574469562833983	22.38540881345569
88-89	22.32612044577418	27.78060245966118	27.483454096633224	22.409822997931414
90-91	22.323986641727263	27.844738453182583	27.51649590508905	22.314779000001106
92-93	22.361979293597987	27.77792515683218	27.528567787218027	22.331527762351808
94-95	22.266226005230262	27.902078962416837	27.50065081047841	22.331044221874492
96-97	22.343796423226543	28.005077916763994	27.60337649046969	22.047749169539774
98-99	23.45931871674353	29.894244966944655	27.788319424411263	18.85811689190055
100	22.330066698377227	30.935347904168527	21.71992718122362	25.01465821623063
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1998.0
1	1735.0
2	2084.5
3	2908.5
4	4012.0
5	6523.5
6	8676.0
7	10244.0
8	11299.0
9	11225.5
10	10414.5
11	9656.0
12	10679.0
13	14088.0
14	21893.0
15	32487.0
16	41197.0
17	46774.5
18	48518.0
19	46745.5
20	42888.0
21	38252.0
22	35374.5
23	36444.0
24	41746.0
25	51477.0
26	64966.0
27	80384.0
28	95866.0
29	112688.5
30	129486.0
31	145803.5
32	163607.5
33	180939.0
34	199134.5
35	216295.5
36	234680.5
37	251934.0
38	263923.5
39	274747.5
40	287966.5
41	299407.5
42	306548.0
43	314334.0
44	320307.5
45	325513.5
46	334075.5
47	346537.5
48	371208.5
49	373544.5
50	353906.0
51	344452.5
52	327928.0
53	302058.5
54	274004.5
55	247837.0
56	225425.0
57	204413.0
58	182952.5
59	160611.5
60	135298.5
61	109695.5
62	86715.5
63	67285.5
64	50733.0
65	37621.5
66	28247.0
67	21416.0
68	16174.0
69	11770.5
70	8551.0
71	6277.0
72	4349.5
73	2974.5
74	2090.5
75	1378.0
76	888.0
77	560.5
78	370.0
79	236.0
80	162.0
81	116.0
82	84.5
83	59.5
84	41.0
85	29.0
86	18.0
87	15.0
88	14.0
89	12.0
90	9.0
91	6.5
92	5.5
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0012948594818490296
4	0.03318539872053085
5	0.0024417350229153133
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	3.083056747174116E-5
22-23	0.0
24-25	0.003361274674300194
26-27	0.04072909396643693
28-29	0.08666744686503458
30-31	0.08483511067493749
32-33	0.03834253019458772
34-35	0.002384492157194168
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	2.6637085295543123E-5
56-57	1.1427157917944939E-4
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	2.1710375200151568E-5
72-73	0.011521677170924045
74-75	0.024868584864983667
76-77	0.003418716203098143
78-79	0.0
80-81	0.0
82-83	0.005108211095339012
84-85	0.040801309143755383
86-87	0.042266412598133817
88-89	0.010623048024703671
90-91	1.1064964938287529E-4
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	94.0
20-21	370.0
22-23	953.0
24-25	2079.0
26-27	3542.0
28-29	6718.0
30-31	15965.0
32-33	20851.0
34-35	28555.0
36-37	41428.0
38-39	49377.0
40-41	50081.0
42-43	58950.0
44-45	69717.0
46-47	68287.0
48-49	60848.0
50-51	54530.0
52-53	53308.0
54-55	65459.0
56-57	75615.0
58-59	72132.0
60-61	73874.0
62-63	75034.0
64-65	79497.0
66-67	91147.0
68-69	66695.0
70-71	57903.0
72-73	54698.0
74-75	55559.0
76-77	55135.0
78-79	58441.0
80-81	59886.0
82-83	57354.0
84-85	57199.0
86-87	60048.0
88-89	64389.0
90-91	68086.0
92-93	72481.0
94-95	87781.0
96-97	256100.0
98-99	491525.0
100-101	5367297.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46539160833976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.55765765764848	76.54080283236556
2	7.464186578943448	12.758592580142311
3	1.7276933242274375	4.429739596026368
4	0.6017642913203618	2.0572008325443907
5	0.27387620066208335	1.1703468370894599
6	0.13762429126802242	0.7057268368825049
7	0.07323684045545745	0.43814506747782195
8	0.04467409261227289	0.30544710558841126
9	0.03061835050686575	0.23551283848236207
>10	0.0866290836531358	1.124300579913017
>50	0.0015091567094532169	0.08278555406008978
>100	4.419399604018732E-4	0.07299220085969092
>500	4.409641144024748E-5	0.021500530635785955
>1k	4.4095621176099934E-5	0.056906607932168896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2331995065228855E-5	0.0	0.0	1.2331995065228855E-5
3	0.0	1.2331995065228855E-5	0.0	2.466399013045771E-5	1.2331995065228855E-5
4	0.0	1.2331995065228855E-5	0.0	2.466399013045771E-5	1.2331995065228855E-5
5	0.0	1.2331995065228855E-5	0.0	2.466399013045771E-5	1.2331995065228855E-5
6	0.0	2.466399013045771E-5	0.0	2.466399013045771E-5	1.2331995065228855E-5
7	0.0	2.466399013045771E-5	0.0	2.466399013045771E-5	1.2331995065228855E-5
8	0.0	3.699598519568656E-5	0.0	2.466399013045771E-5	2.466399013045771E-5
9	3.699598519568656E-5	1.603159358479751E-4	0.0	6.165997532614427E-5	4.932798026091542E-5
10-11	4.932798026091542E-5	1.603159358479751E-4	0.0	7.399197039137312E-5	4.932798026091542E-5
12-13	4.932798026091542E-5	1.7264793091320397E-4	0.0	7.399197039137312E-5	4.932798026091542E-5
14-15	4.932798026091542E-5	2.528058988371915E-4	0.0	9.865596052183084E-5	7.399197039137314E-5
16-17	4.932798026091542E-5	2.836358865002637E-4	0.0	1.4181794325013184E-4	9.24899629892164E-5
18-19	4.932798026091542E-5	3.2063187169595027E-4	0.0	1.7264793091320397E-4	1.2331995065228854E-4
20-21	4.932798026091542E-5	3.699598519568656E-4	0.0	1.7264793091320397E-4	1.2331995065228854E-4
22-23	4.932798026091542E-5	4.007898396199378E-4	0.0	2.219759111741194E-4	1.5414993831536068E-4
24-25	5.5493977793529845E-5	4.562838174134676E-4	0.0	2.651378939024204E-4	1.9731192104366168E-4
26-27	6.78259728587587E-5	4.8094780754392535E-4	0.0	3.2679786922856465E-4	1.9731192104366168E-4
28-29	7.399197039137312E-5	4.932798026091542E-4	0.0	4.994458001417687E-4	1.9731192104366168E-4
30-31	9.865596052183084E-5	5.672717730005274E-4	0.0	0.001054385578077067	1.9731192104366168E-4
32-33	1.2331995065228854E-4	5.672717730005274E-4	0.0	0.00231224907473041	2.2197591117411938E-4
34-35	1.2948594818490297E-4	6.720937310549725E-4	0.0	0.003853748457884017	2.2197591117411938E-4
36-37	1.356519457175174E-4	7.830816866420322E-4	0.0	0.006400305438853776	2.2197591117411938E-4
38-39	1.4798394078274624E-4	8.015796792398756E-4	0.0	0.01019239392141165	2.2197591117411938E-4
40-41	1.5414993831536068E-4	8.879036446964775E-4	0.0	0.014212624312676255	2.2197591117411938E-4
42-43	1.603159358479751E-4	9.310656274247786E-4	0.0	0.01791222283224491	2.281419087067338E-4
44-45	1.603159358479751E-4	0.0010482195805444528	0.0	0.021938619221042132	2.3430790623934824E-4
46-47	1.603159358479751E-4	0.0011222115509358257	0.0	0.026637109340894326	2.4047390377196265E-4
48-49	1.603159358479751E-4	0.0011222115509358257	0.0	0.030984137601387497	2.713038914350348E-4
50-51	1.603159358479751E-4	0.001282527486783801	0.0	0.0353866598396742	2.713038914350348E-4
52-53	1.603159358479751E-4	0.0012948594818490298	0.0	0.03964736413471077	3.144658741633358E-4
54-55	1.603159358479751E-4	0.0013010254793816441	0.0	0.04386490644701904	3.6379385442425125E-4
56-57	1.6648193338058954E-4	0.0013318554670447163	0.0	0.04811327874699038	3.7612584948948006E-4
58-59	1.7264793091320397E-4	0.001356519457175174	0.0	0.05254046497540754	3.884578445547089E-4
60-61	1.7264793091320397E-4	0.0013688514522404028	0.0	0.0570971371520096	3.9462384208732335E-4
62-63	1.7881392844581838E-4	0.0014366774250991615	0.0	0.06161681334341597	4.192878322177811E-4
64-65	1.849799259784328E-4	0.0016278233486102089	0.0	0.06586518564338731	4.3161982728300995E-4
66-67	2.0964391610889054E-4	0.0016401553436754376	0.0	0.07006422996309775	4.501178198808532E-4
68-69	2.0964391610889054E-4	0.001689483323936353	0.0	0.07481204806321085	4.562838174134676E-4
70-71	2.0964391610889054E-4	0.0017018153190015818	0.0	0.07936872023981291	4.8094780754392535E-4
72-73	2.0964391610889054E-4	0.0017079813165341963	0.0	0.0826182009395007	5.179437927396119E-4
74-75	2.0964391610889054E-4	0.001732645306664654	0.0	0.08542372981684027	5.179437927396119E-4
76-77	2.2197591117411938E-4	0.0018744632499147857	0.0	0.08835874464236473	5.179437927396119E-4
78-79	2.3430790623934822E-4	0.0019114592351104724	0.0	0.09025787188241	5.179437927396119E-4
80-81	2.466399013045771E-4	0.0019114592351104724	0.0	0.09091763361839973	5.487737804026841E-4
82-83	2.466399013045771E-4	0.001917625232643087	0.0	0.09144174340867196	6.042677581962138E-4
84-85	2.5897189636980595E-4	0.0019299572277083157	0.0	0.09159589334698731	6.042677581962138E-4
86-87	2.5897189636980595E-4	0.0019607872153713877	0.0	0.09172537929517222	6.042677581962138E-4
88	2.5897189636980595E-4	0.002022447190697532	0.0	0.09173771129023744	6.289317483266716E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9860	0.0	51.88081	1
GGTATCA	10785	0.0	51.656895	1
TATACAC	13360	0.0	40.457207	3
GTATCAA	19455	0.0	38.510155	1
TTATACA	14020	0.0	37.519726	2
TCAACGC	23890	0.0	30.576376	4
ATCAACG	23900	0.0	30.54595	3
CAACGCA	24310	0.0	30.022444	5
TATCAAC	24810	0.0	29.786085	2
TACACAT	18510	0.0	29.422821	5
AACGCAG	24920	0.0	29.389524	6
ACACATC	18775	0.0	27.804985	6
ACATCTC	19780	0.0	26.41377	8
ACGCAGA	28865	0.0	25.284327	7
CACATCT	20630	0.0	25.098427	7
CGCAGAG	29255	0.0	24.961815	8
ATACACA	24350	0.0	23.841572	4
GTGGTAT	5010	0.0	23.634573	1
TGGTATC	4910	0.0	23.508694	2
CATCTCC	22815	0.0	22.657415	9
>>END_MODULE