FastQCFastQC Report
Fri 27 May 2016
SRR2139277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8151094
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106400.048.8679351
CTTATAC69350.041.5020681
GTATCAA188450.036.7957461
TATACAC104900.030.7852573
TCAACGC224700.030.1158854
ATCAACG225500.029.9904163
CAACGCA233250.028.9112785
TATCAAC238800.028.5532552
AACGCAG239150.028.2871846
TTATACA111500.027.042372
ACGCAGA275600.024.4994057
CGCAGAG280600.024.0781088
GTGGTAT52450.023.8442841
ACACATC122800.023.604426
ACATCTC132250.022.638
TACACAT140300.022.4914885
TGGTATC52500.021.7004762
GTACATG230650.020.8532241
TACATGG223950.020.8268572
GCAGAGT328050.020.6475949
Produced by FastQC (version 0.11.5)