##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139277_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8151094
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.792296960383474	33.0	33.0	33.0	33.0	33.0
2	32.79401771099683	33.0	33.0	33.0	33.0	33.0
3	32.79589083379483	33.0	33.0	33.0	33.0	33.0
4	36.73340719662907	37.0	37.0	37.0	37.0	37.0
5	36.75328710477391	37.0	37.0	37.0	37.0	37.0
6	36.75882022707627	37.0	37.0	37.0	37.0	37.0
7	36.74441970611552	37.0	37.0	37.0	37.0	37.0
8	36.73887456088716	37.0	37.0	37.0	37.0	37.0
9	36.73798768607011	37.0	37.0	37.0	37.0	37.0
10-11	36.6927065863797	37.0	37.0	37.0	37.0	37.0
12-13	36.692677326503656	37.0	37.0	37.0	37.0	37.0
14-15	38.972009156071564	40.0	40.0	40.0	37.0	40.0
16-17	38.91548152922786	40.0	40.0	40.0	37.0	40.0
18-19	38.936809647882846	40.0	40.0	40.0	37.0	40.0
20-21	38.978676134988405	40.0	40.0	40.0	37.0	40.0
22-23	39.01049335440325	40.0	40.0	40.0	37.0	40.0
24-25	39.02010281339773	40.0	40.0	40.0	37.0	40.0
26-27	38.92862865074193	40.0	40.0	40.0	37.0	40.0
28-29	38.857576964136115	40.0	40.0	40.0	37.0	40.0
30-31	38.79209578119536	40.0	37.0	40.0	37.0	40.0
32-33	38.73288198921763	40.0	37.0	40.0	37.0	40.0
34-35	38.723083124092966	40.0	37.0	40.0	37.0	40.0
36-37	38.6908908973193	40.0	37.0	40.0	37.0	40.0
38-39	38.644737028168464	40.0	37.0	40.0	37.0	40.0
40-41	38.60396103002305	40.0	37.0	40.0	37.0	40.0
42-43	38.483581309386835	40.0	37.0	40.0	37.0	40.0
44-45	38.44546910876534	40.0	37.0	40.0	37.0	40.0
46-47	38.380463393585345	40.0	37.0	40.0	37.0	40.0
48-49	38.31475539227338	40.0	37.0	40.0	37.0	40.0
50-51	38.25800406199953	40.0	37.0	40.0	37.0	40.0
52-53	38.22265309751743	40.0	37.0	40.0	37.0	40.0
54-55	38.13871891215649	40.0	37.0	40.0	37.0	40.0
56-57	38.03995972965852	40.0	37.0	40.0	37.0	40.0
58-59	37.79198952848384	40.0	37.0	40.0	33.0	40.0
60-61	37.71172333815363	40.0	37.0	40.0	33.0	40.0
62-63	37.36953192297248	37.0	37.0	40.0	33.0	40.0
64-65	37.18365671100584	37.0	37.0	40.0	33.0	40.0
66-67	37.033673062369076	37.0	37.0	40.0	33.0	40.0
68-69	36.8088863505611	37.0	37.0	40.0	33.0	40.0
70-71	36.53512030282603	37.0	37.0	40.0	33.0	40.0
72-73	36.293612224704205	37.0	37.0	37.0	33.0	40.0
74-75	36.04384471569782	37.0	37.0	37.0	33.0	40.0
76-77	34.25244406977069	35.0	33.0	37.0	30.0	37.0
78-79	35.62382461572534	37.0	35.0	37.0	33.0	37.0
80-81	35.76194441800136	37.0	37.0	37.0	33.0	37.0
82-83	35.70471037288665	37.0	37.0	37.0	33.0	37.0
84-85	35.28045881257205	37.0	35.0	37.0	33.0	37.0
86-87	35.371632424565675	37.0	33.0	37.0	33.0	37.0
88-89	35.37456812129611	37.0	33.0	37.0	33.0	37.0
90-91	35.319327795228546	37.0	33.0	37.0	33.0	37.0
92-93	35.24621031072407	37.0	33.0	37.0	33.0	37.0
94-95	35.20244916622109	37.0	33.0	37.0	33.0	37.0
96-97	35.16581579019504	37.0	33.0	37.0	33.0	37.0
98-99	35.12995399593723	37.0	33.0	37.0	33.0	37.0
100	35.05976538173223	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	33.0
21	126.0
22	516.0
23	1553.0
24	3875.0
25	8119.0
26	15197.0
27	24280.0
28	37638.0
29	53219.0
30	72790.0
31	99257.0
32	134035.0
33	183248.0
34	261508.0
35	401571.0
36	775178.0
37	2294574.0
38	3639514.0
39	144857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39644322590318	20.108012495009874	14.485957345112203	24.009586933974752
2	14.412629764789855	20.70863125857707	42.135264297037914	22.743474679595156
3	18.191659710454733	25.762287958220526	30.480783433861024	25.565268897463717
4	12.335627744957906	18.201736327199537	38.520232748990004	30.94240317885255
5	13.452419101946834	37.448465661850335	35.46504694710115	13.634068289101686
6	29.9017039921267	37.62591868036364	18.79385760978833	13.678519717721327
7	26.44751735165856	32.67471826481206	22.577030764213983	18.300733619315395
8	24.283562427325705	36.68868252531501	21.20977871191278	17.817976335446506
9	25.1749519757716	17.10306616510618	21.10087308525702	36.6211087738652
10-11	24.059328723236415	26.7910356572995	28.734566182159107	20.415069437304982
12-13	24.97427952615931	24.242156942368716	28.796465848633325	21.987097682838648
14-15	22.11628893005037	25.770197227513265	26.439076766873253	25.674437075563105
16-17	21.72164865231587	29.077188902495788	27.067587491936667	22.133574953251674
18-19	21.70426448277986	27.907395007345027	28.955714410850863	21.432626099024255
20-21	22.388915389016972	27.037872118233757	28.83441517652521	21.738797316224066
22-23	22.117423742588482	27.240224702957995	28.735911205388643	21.906440349064884
24-25	22.00923910385761	27.382796103620947	28.82141080621363	21.78655398630781
26-27	21.88774888725821	27.44870115454021	28.845479434291992	21.818070523909583
28-29	21.617538095708362	27.33178219695212	29.0783008819059	21.972378825433616
30-31	22.037184149234623	27.17802479230353	28.712026581408395	22.072764477053457
32-33	22.02838639218015	27.327925832383244	28.898206742379568	21.745481033057043
34-35	22.030237367639206	27.622901392723676	28.78872040822589	21.558140831411233
36-37	21.91727844969222	27.407090507121108	28.6360806756707	22.03955036751597
38-39	21.830812736695236	27.400681433127367	28.845481612517442	21.923024217659957
40-41	22.041588787593795	27.283677137343943	28.77005248422469	21.90468159083757
42-43	22.086844354190198	27.30904307416165	28.67018065595237	21.93393191569578
44-45	22.007936090968556	27.59986834380715	28.433214435754685	21.95898112946961
46-47	22.32142124750199	27.372422142632296	27.91422975216272	22.39192685770299
48-49	22.131563557172537	27.514767700723443	28.057487568377958	22.296181173726062
50-51	22.035928876695532	27.720420297511716	28.02545099916958	22.218199826623174
52-53	22.15609212653353	27.766240265654357	28.071646540829953	22.006021066982157
54-55	22.147840367401635	27.732656267524874	27.866534364124263	22.25296900094923
56-57	22.2893700285654	27.970891683278182	27.59915483740057	22.140583450755848
58-59	22.080787264678143	28.135307760209166	27.865611878782182	21.91829309633051
60-61	22.318355708491776	28.255319840315092	27.534686516481976	21.89163793471116
62-63	22.136711911712208	28.42787693180083	27.427071693270765	22.008339463216195
64-65	22.155327161408355	28.28455169704967	27.47620409386747	22.083917047674504
66-67	22.235756857328713	28.152474055593828	27.434555345479893	22.177213741597562
68-69	22.25753825679163	27.962249572815416	27.577047014734664	22.203165155658294
70-71	22.247031587744704	27.739486106416457	27.62823757964738	22.385244726191463
72-73	22.29272060549834	27.771516013529418	27.56691710783678	22.36884627313546
74-75	22.155933310950523	27.87025486629683	27.58316179146021	22.390650031292438
76-77	22.28474667820204	27.87744043787905	27.458883429354852	22.37892945456406
78-79	22.238280351036117	27.911124010383915	27.47671901132937	22.373876627250596
80-81	22.249967574688764	27.879097734842446	27.44779303053244	22.42314165993635
82-83	22.307653276324576	27.857351256766993	27.53259535348787	22.30240011342056
84-85	22.454446540033636	27.80189663373268	27.43830638638588	22.305350439847803
86-87	22.21747117939914	27.809178396684903	27.596247751845716	22.37710267207024
88-89	22.28908130915187	27.832048957736642	27.547087550367394	22.331782182744092
90-91	22.304058086680676	27.850333859068595	27.582431302501032	22.263176751749704
92-93	22.34735522294491	27.81535929786067	27.574712013165115	22.26257346602931
94-95	22.20821329363238	27.917605288679916	27.564883312605517	22.309298105082195
96-97	22.343627310542555	27.98764809917944	27.64474424650394	22.02398034377407
98-99	23.385894059381908	29.898569276595637	27.85954789650291	18.855988767519538
100	22.290709570646413	30.946492068102433	21.699347384887545	25.06345097636361
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1857.0
1	1598.0
2	1900.0
3	2627.5
4	3677.0
5	6117.5
6	8101.0
7	9400.5
8	10282.0
9	10200.5
10	9641.5
11	9214.0
12	10199.5
13	13478.5
14	21101.0
15	31457.5
16	39974.5
17	45160.5
18	46559.0
19	44306.5
20	40089.5
21	35891.0
22	33730.0
23	35115.0
24	40638.0
25	50250.0
26	63812.5
27	79391.5
28	94897.5
29	112335.0
30	129573.0
31	146002.5
32	164835.5
33	183341.5
34	201966.0
35	219732.5
36	238503.5
37	257770.5
38	272562.0
39	284327.5
40	296621.0
41	308171.0
42	316270.0
43	323974.0
44	330493.0
45	336660.0
46	343565.0
47	350228.5
48	361624.0
49	359906.5
50	345116.0
51	332518.0
52	314678.0
53	291244.0
54	266871.5
55	243629.5
56	222407.0
57	200683.0
58	177660.5
59	155093.0
60	130775.0
61	107019.5
62	84627.5
63	64589.0
64	48120.5
65	35576.5
66	26721.5
67	20670.0
68	15918.5
69	11576.0
70	8219.0
71	5857.5
72	4155.0
73	2894.5
74	2013.5
75	1388.0
76	926.0
77	572.0
78	348.0
79	225.5
80	148.0
81	97.5
82	75.5
83	54.0
84	33.5
85	23.0
86	17.0
87	14.5
88	9.5
89	5.5
90	6.0
91	5.5
92	3.0
93	1.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0013004389349454196
4	0.03367646109835073
5	0.0026744876209254856
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	1.8402863338312533E-5
22-23	0.0
24-25	0.003215034581562329
26-27	0.040738087239404815
28-29	0.08873430993877689
30-31	0.08707868586904934
32-33	0.0394235151476636
34-35	0.002204048188272389
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.32029663632647E-5
56-57	9.317037834161385E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	3.546107590180884E-5
72-73	0.011233441935189546
74-75	0.024289806072927224
76-77	0.0032558461333629463
78-79	0.0
80-81	0.0
82-83	0.004715685219654678
84-85	0.041161829524383645
86-87	0.04302962268603742
88-89	0.010706670961606308
90-91	9.300010671762246E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	110.0
20-21	391.0
22-23	926.0
24-25	1846.0
26-27	3179.0
28-29	6017.0
30-31	14818.0
32-33	19248.0
34-35	26657.0
36-37	39121.0
38-39	46421.0
40-41	48420.0
42-43	56997.0
44-45	67553.0
46-47	66706.0
48-49	59738.0
50-51	53178.0
52-53	51921.0
54-55	58207.0
56-57	64975.0
58-59	63055.0
60-61	64184.0
62-63	64806.0
64-65	68018.0
66-67	76357.0
68-69	63409.0
70-71	58494.0
72-73	55273.0
74-75	57184.0
76-77	56876.0
78-79	59744.0
80-81	61467.0
82-83	59126.0
84-85	58828.0
86-87	62260.0
88-89	66431.0
90-91	70403.0
92-93	74611.0
94-95	91104.0
96-97	260872.0
98-99	501404.0
100-101	5470759.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.87922334116874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.22614523970535	75.7344590967251
2	7.581974867662922	12.871042763189788
3	1.8262902261960299	4.6504228798525915
4	0.6592932868589899	2.238412085705497
5	0.29749465548342907	1.262555765279101
6	0.15132335540189717	0.770652532793561
7	0.07910045283025366	0.4699789501516679
8	0.050334484198213424	0.3417881540818149
9	0.031000308456939178	0.23681538946454966
>10	0.09530078479439381	1.210073053805065
>50	0.001320634269934112	0.07417354458921448
>100	3.0883333272949175E-4	0.05390073915099571
>500	6.772285276082974E-5	0.03347404195290741
>1k	4.5147956143572886E-5	0.0522510032581837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.680487551732319E-5	0.0	1.2268291839107732E-5	0.0
3	0.0	4.907316735643093E-5	0.0	3.680487551732319E-5	0.0
4	0.0	1.1041462655196959E-4	0.0	4.907316735643093E-5	0.0
5	0.0	1.1041462655196959E-4	0.0	4.907316735643093E-5	0.0
6	0.0	1.2268291839107731E-4	0.0	6.134145919553866E-5	0.0
7	0.0	1.2268291839107731E-4	0.0	8.587804287375413E-5	0.0
8	0.0	1.3495121023018506E-4	0.0	1.2268291839107731E-4	0.0
9	0.0	1.7175608574750826E-4	0.0	1.594877939084005E-4	0.0
10-11	0.0	1.7175608574750826E-4	0.0	1.8402437758661597E-4	0.0
12-13	6.134145919553866E-6	1.7175608574750826E-4	0.0	1.962926694257237E-4	0.0
14-15	1.2268291839107732E-5	2.4536583678215463E-4	0.0	2.330975449430469E-4	0.0
16-17	1.2268291839107732E-5	2.76036566379924E-4	0.0	2.514999827017085E-4	0.0
18-19	1.2268291839107732E-5	3.1284144189724716E-4	0.0	2.699024204603701E-4	0.0
20-21	1.2268291839107732E-5	3.435121714950165E-4	0.0	2.8217071229947785E-4	0.0
22-23	1.2268291839107732E-5	3.741829010927858E-4	0.0	3.251097337363549E-4	6.134145919553866E-6
24-25	1.2268291839107732E-5	4.2939021436877065E-4	0.0	3.987194847710013E-4	1.2268291839107732E-5
26-27	1.2268291839107732E-5	4.723292358056477E-4	0.0	4.539267980469861E-4	1.2268291839107732E-5
28-29	1.2268291839107732E-5	4.907316735643093E-4	0.0	6.011463001162789E-4	1.2268291839107732E-5
30-31	1.2268291839107732E-5	5.95012154196725E-4	0.0	0.0012452316216694348	1.2268291839107732E-5
32-33	1.2268291839107732E-5	6.256828837944943E-4	0.0	0.002508865681097531	2.4536583678215464E-5
34-35	1.2268291839107732E-5	7.176950725878023E-4	0.0	0.004257097268170383	2.4536583678215464E-5
36-37	1.2268291839107732E-5	8.219755532202181E-4	0.0	0.006778231241107022	2.4536583678215464E-5
38-39	1.2268291839107732E-5	8.894511583353106E-4	0.0	0.010385109041804695	2.4536583678215464E-5
40-41	1.2268291839107732E-5	0.0010428048063241572	0.0	0.014261889262962738	2.4536583678215464E-5
42-43	1.2268291839107732E-5	0.001098012119600142	0.0	0.018322693861707397	2.4536583678215464E-5
44-45	1.2268291839107732E-5	0.0012268291839107734	0.0	0.022555254546199565	2.4536583678215464E-5
46-47	1.2268291839107732E-5	0.0013617803941409583	0.0	0.027223339590980056	2.4536583678215464E-5
48-49	1.2268291839107732E-5	0.0013924511237387277	0.0	0.03246190020627906	2.4536583678215464E-5
50-51	1.2268291839107732E-5	0.0014905974584515894	0.0	0.03748576571439367	2.4536583678215464E-5
52-53	1.2268291839107732E-5	0.001551938917647128	0.0	0.04215998490509372	4.293902143687706E-5
54-55	1.2268291839107732E-5	0.001607146230923113	0.0	0.046828069949874214	6.134145919553866E-5
56-57	1.2268291839107732E-5	0.0016439511064404362	0.0	0.05182126472839106	6.134145919553866E-5
58-59	1.2268291839107732E-5	0.001699158419716421	0.0	0.05667950829667772	6.134145919553866E-5
60-61	1.2268291839107732E-5	0.0017298291493141901	0.0	0.06187512989053985	6.134145919553866E-5
62-63	1.2268291839107732E-5	0.0017420974411532979	0.0	0.06703394660888465	6.134145919553866E-5
64-65	1.2268291839107732E-5	0.0019199876728203601	0.0	0.07157934873527405	6.134145919553866E-5
66-67	1.2268291839107732E-5	0.0019506584024181295	0.0	0.07662161668114734	6.134145919553866E-5
68-69	1.2268291839107732E-5	0.0019813291320158986	0.0	0.08178656754541169	6.134145919553866E-5
70-71	1.2268291839107732E-5	0.002005865715694114	0.0	0.08657733550858326	6.134145919553866E-5
72-73	1.2268291839107732E-5	0.002011999861613668	0.0	0.09028849378991335	7.360975103464639E-5
74-75	1.2268291839107732E-5	0.002054938883050545	0.0	0.09298751799451704	7.360975103464639E-5
76-77	1.2268291839107732E-5	0.0021408169259242993	0.0	0.09571107878279897	7.360975103464639E-5
78-79	1.2268291839107732E-5	0.002146951071843853	0.0	0.09753292012090647	7.974389695420026E-5
80-81	1.2268291839107732E-5	0.002159219363682961	0.0	0.09818927373429873	9.201218879330799E-5
82-83	1.2268291839107732E-5	0.0021898900932807303	0.0	0.0985266517598742	9.814633471286186E-5
84-85	1.2268291839107732E-5	0.0022266949687980535	0.0	0.09867387126194349	9.814633471286186E-5
86-87	1.2268291839107732E-5	0.0023187071575913615	0.0	0.09873521272113903	9.814633471286186E-5
88	1.2268291839107732E-5	0.0023800486167869	0.0	0.09874748101297813	9.814633471286186E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10640	0.0	48.867935	1
CTTATAC	6935	0.0	41.502068	1
GTATCAA	18845	0.0	36.795746	1
TATACAC	10490	0.0	30.785257	3
TCAACGC	22470	0.0	30.115885	4
ATCAACG	22550	0.0	29.990416	3
CAACGCA	23325	0.0	28.911278	5
TATCAAC	23880	0.0	28.553255	2
AACGCAG	23915	0.0	28.287184	6
TTATACA	11150	0.0	27.04237	2
ACGCAGA	27560	0.0	24.499405	7
CGCAGAG	28060	0.0	24.078108	8
GTGGTAT	5245	0.0	23.844284	1
ACACATC	12280	0.0	23.60442	6
ACATCTC	13225	0.0	22.63	8
TACACAT	14030	0.0	22.491488	5
TGGTATC	5250	0.0	21.700476	2
GTACATG	23065	0.0	20.853224	1
TACATGG	22395	0.0	20.826857	2
GCAGAGT	32805	0.0	20.647594	9
>>END_MODULE