FastQCFastQC Report
Fri 27 May 2016
SRR2139276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5861381
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76600.051.8208731
GTATCAA137850.037.4991461
TCAACGC164750.030.6013874
ATCAACG165000.030.5294933
CAACGCA170700.029.506535
TATCAAC173000.029.3636512
AACGCAG173850.029.0428316
ACGCAGA201250.025.1098147
CGCAGAG202700.024.9301938
GCAGAGT240650.020.9280519
TGGTATC32100.020.8087752
GTACATG172350.020.7357541
GAGTACT144650.020.48168212-13
TACATGG172200.020.259682
GTGGTAT37200.020.1289711
ACATGGG174300.019.5274033
CAGAGTA232950.019.23688710-11
AGAGTAC217850.018.38339410-11
GTACTTT160850.018.36645114-15
CATGGGG122900.017.4472434
Produced by FastQC (version 0.11.5)