##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5861381
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.713375909192735	33.0	33.0	33.0	33.0	33.0
2	32.70468444211355	33.0	33.0	33.0	33.0	33.0
3	32.70479090166635	33.0	33.0	33.0	33.0	33.0
4	36.62852969291708	37.0	37.0	37.0	37.0	37.0
5	36.64859646557697	37.0	37.0	37.0	37.0	37.0
6	36.57097499718923	37.0	37.0	37.0	37.0	37.0
7	36.62020981062313	37.0	37.0	37.0	37.0	37.0
8	36.62328587750907	37.0	37.0	37.0	37.0	37.0
9	36.63318593348564	37.0	37.0	37.0	37.0	37.0
10-11	36.59402016350754	37.0	37.0	37.0	37.0	37.0
12-13	36.567353666311746	37.0	37.0	37.0	37.0	37.0
14-15	38.6700373171442	40.0	37.0	40.0	37.0	40.0
16-17	38.615663697684894	40.0	37.0	40.0	37.0	40.0
18-19	38.645283679733495	40.0	37.0	40.0	37.0	40.0
20-21	38.671015090940024	40.0	37.0	40.0	37.0	40.0
22-23	38.52332728241355	40.0	37.0	40.0	37.0	40.0
24-25	38.67761087926071	40.0	37.0	40.0	37.0	40.0
26-27	38.47139514156609	40.0	37.0	40.0	37.0	40.0
28-29	38.45905019420485	40.0	37.0	40.0	37.0	40.0
30-31	38.44173141144276	40.0	37.0	40.0	37.0	40.0
32-33	38.43702733658624	40.0	37.0	40.0	37.0	40.0
34-35	38.349002397870535	40.0	37.0	40.0	37.0	40.0
36-37	33.28944591945681	40.0	37.0	40.0	8.5	40.0
38-39	37.281528184385145	40.0	37.0	40.0	33.0	40.0
40-41	37.855460838503134	40.0	37.0	40.0	35.0	40.0
42-43	38.071749162555875	40.0	37.0	40.0	35.0	40.0
44-45	38.08003052740503	40.0	37.0	40.0	35.0	40.0
46-47	37.97998065612505	40.0	37.0	40.0	33.0	40.0
48-49	37.96363521403485	40.0	37.0	40.0	33.0	40.0
50-51	37.92321621174253	40.0	37.0	40.0	33.0	40.0
52-53	37.85373099203813	40.0	37.0	40.0	33.0	40.0
54-55	37.77576710447413	40.0	37.0	40.0	33.0	40.0
56-57	37.701887530700176	40.0	37.0	40.0	33.0	40.0
58-59	37.561666110275475	40.0	37.0	40.0	33.0	40.0
60-61	37.37484552030874	38.5	37.0	40.0	33.0	40.0
62-63	37.15682960573389	37.0	37.0	40.0	33.0	40.0
64-65	36.94884759738537	37.0	37.0	40.0	33.0	40.0
66-67	36.681082660794104	37.0	37.0	40.0	33.0	40.0
68-69	36.40179216416789	37.0	37.0	40.0	33.0	40.0
70-71	36.130043149627454	37.0	37.0	38.5	33.0	40.0
72-73	35.86817106697895	37.0	37.0	37.0	33.0	40.0
74-75	35.58198303771317	37.0	35.0	37.0	33.0	40.0
76-77	33.75616815367005	35.0	33.0	37.0	27.0	37.0
78-79	35.202082030072255	37.0	33.0	37.0	33.0	37.0
80-81	35.224623012727335	37.0	33.0	37.0	33.0	37.0
82-83	35.29315638116087	37.0	33.0	37.0	33.0	37.0
84-85	35.13895915193781	37.0	33.0	37.0	33.0	37.0
86-87	35.06745124668937	37.0	33.0	37.0	33.0	37.0
88-89	34.97711383364248	37.0	33.0	37.0	33.0	37.0
90-91	34.840331948159886	37.0	33.0	37.0	33.0	37.0
92-93	34.82263112233237	37.0	33.0	37.0	33.0	37.0
94-95	34.7850086436415	37.0	33.0	37.0	33.0	37.0
96-97	34.76119496306934	37.0	33.0	37.0	33.0	37.0
98-99	34.72209048568712	37.0	33.0	37.0	33.0	37.0
100	34.6688644527838	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	30.0
21	160.0
22	636.0
23	1949.0
24	4672.0
25	9379.0
26	16454.0
27	26875.0
28	39911.0
29	56983.0
30	77332.0
31	104041.0
32	139088.0
33	189186.0
34	264000.0
35	408784.0
36	787243.0
37	1818518.0
38	1857027.0
39	59107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84689580834278	20.074552396440364	14.272182613619556	23.806369181597304
2	14.60394743150121	20.9744427123915	41.8995796383139	22.522030217793386
3	18.323332922158876	25.898428989403126	30.265920683593745	25.512317404844246
4	12.395140193868746	18.002519553155558	39.019203445129754	30.583136807845936
5	13.457098339297158	37.58103715270645	35.46589836495878	13.495966143037613
6	29.943097027816485	37.84792355248703	18.816111766152037	13.392867653544446
7	26.501553132273774	32.73419694095982	22.94681065776137	17.817439269005035
8	24.168621695126113	36.90526174633589	21.213754915437164	17.71236164310083
9	25.472358817828084	17.061900599875695	21.327738292392183	36.13800228990404
10-11	24.086431166989485	26.920797334280095	28.82297704244102	20.1697944562894
12-13	24.94250928236878	24.47318302632093	29.021701540984967	21.562606150325326
14-15	22.054017645329658	25.86410949910951	26.850873539870552	25.230999315690276
16-17	21.51611540010793	29.01401563897655	27.49912520615875	21.97074375475677
18-19	21.495574165883433	28.08768104308524	29.318628152648667	21.09811663838266
20-21	22.36087695755242	27.225844558699723	29.25605437005112	21.15722411369673
22-23	22.08992183462301	27.185967940439316	29.061341504260778	21.66276872067689
24-25	21.807252581583395	27.330422024824223	29.195276737855437	21.667048655736945
26-27	21.79580970960597	27.45822952725564	29.010680050841287	21.73528071229711
28-29	21.67035745206751	27.556483546162454	29.13025381378784	21.642905187982198
30-31	22.031444952937292	27.429241668981735	28.974324509662413	21.564988868418556
32-33	21.79199217076239	27.553611767165876	28.924396781922823	21.729999280148906
34-35	21.966614187729032	27.566052984629703	28.891643634657605	21.575689192983656
36-37	22.0896106254774	27.524636021655823	28.68448196445942	21.701271388407356
38-39	21.747346334195676	27.680045604816723	28.92504392878078	21.647564132206824
40-41	22.09533756552685	27.555123433070598	28.69561246259297	21.65392653880958
42-43	21.940180492674983	27.633301551019034	28.563451671588364	21.86306628471762
44-45	22.045713752460898	27.768964284319292	28.3484190766652	21.83690288655461
46-47	22.083789804346644	27.72861509443044	28.007632165629225	22.179962935593686
48-49	22.03869093356153	27.846024600130598	28.05457552974866	22.060708936559216
50-51	22.041826101535463	28.024168799204226	27.939271655289716	21.994733443970603
52-53	22.198991169911945	27.905790038832563	27.82539308216217	22.069825709093323
54-55	22.167081657912806	27.823911017169134	27.86508604930474	22.14392127561332
56-57	22.151919607500968	27.95517252942805	27.804887872661933	22.08801999040905
58-59	22.156165068126967	28.01924854907687	27.799701184050058	22.02488519874611
60-61	22.226847972755714	27.966686721654455	27.780792171392715	22.025673134197117
62-63	22.202158770349183	28.008428790000117	27.716865624684868	22.072546814965833
64-65	22.160093610447802	27.95656278916221	27.74802598121723	22.135317619172756
66-67	22.241611894968322	27.964638093648702	27.600162304501534	22.19358770688144
68-69	22.199994143667436	27.986456602787868	27.669576825854218	22.143972427690482
70-71	22.19008784622993	27.779494700036363	27.70857585130873	22.32184160242498
72-73	22.218473515485123	27.82495418802079	27.687162205099963	22.269410091394125
74-75	22.09759787991093	27.97638761289285	27.625524594025336	22.300489913170882
76-77	22.20018246405494	27.94757071779172	27.543152629266327	22.309094188887013
78-79	22.203964872658254	27.95340040369364	27.537494962882757	22.305139760765346
80-81	22.271709814464486	27.918507695409666	27.4888579349028	22.320924555223048
82-83	22.217831706954534	27.92658098473752	27.626945379305713	22.22864192900223
84-85	22.328715022305815	27.927966082436274	27.52547184740567	22.217847047852242
86-87	22.19049538957954	27.9351808092434	27.61963004479397	22.25469375638309
88-89	22.238610177888592	27.89948430555091	27.60409070312538	22.25781481343512
90-91	22.226997247288445	27.941521788790325	27.629145817141847	22.20233514677938
92-93	22.256330089571875	27.907073560041646	27.641098890081516	22.19549746030496
94-95	22.15141923594447	27.98210726380018	27.661783134746106	22.204690365509247
96-97	22.20784302624721	28.10157425554081	27.71977441017452	21.970808308037455
98-99	23.313956092165135	30.0069176341643	27.906321316281552	18.77280495738901
100	22.2487557820874	31.095406271272054	21.810788049250995	24.84504989738955
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1379.0
1	1214.0
2	1536.0
3	2151.5
4	2941.5
5	4845.5
6	6460.0
7	7577.5
8	8349.5
9	8331.0
10	7730.5
11	7125.5
12	7726.0
13	10153.5
14	15689.5
15	23200.0
16	29828.0
17	34172.0
18	35367.0
19	33774.5
20	30883.5
21	27766.5
22	25941.0
23	27097.5
24	31124.5
25	38254.5
26	48128.0
27	59179.0
28	71128.0
29	83733.0
30	96156.0
31	108540.0
32	121897.0
33	135529.5
34	149628.5
35	163414.5
36	178331.0
37	191547.0
38	199762.0
39	207352.0
40	216280.5
41	224604.0
42	230325.5
43	234891.0
44	239058.5
45	242912.5
46	246018.5
47	246611.0
48	244434.5
49	239856.5
50	233513.0
51	224633.0
52	212869.0
53	200206.0
54	186517.0
55	171579.0
56	156708.5
57	141010.5
58	124722.0
59	108315.5
60	90734.5
61	73948.0
62	58563.0
63	44737.0
64	33033.5
65	24284.5
66	18424.0
67	14182.5
68	10723.5
69	7708.0
70	5505.5
71	3876.5
72	2690.5
73	1862.5
74	1286.5
75	879.0
76	582.5
77	374.5
78	231.5
79	152.5
80	105.0
81	72.5
82	57.5
83	42.0
84	25.0
85	15.0
86	11.0
87	10.5
88	9.5
89	8.0
90	5.0
91	3.0
92	3.0
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	0.0
2	0.0
3	0.002081420743677983
4	0.0139557554781032
5	0.008342743800479785
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0015785598465742222
26-27	0.0364714452823763
28-29	0.08472932047802298
30-31	0.08155618781073527
32-33	0.03674174953263278
34-35	0.0013399429167138726
36-37	7.450240228508151
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	1.8344853901583525E-5
56-57	3.69522009423735E-5
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.01091098365807668
74-75	0.0327122201571018
76-77	0.004140389741371303
78-79	0.0
80-81	0.0
82-83	0.003255244987895197
84-85	0.038669524666355315
86-87	0.04188493894697096
88-89	0.012047484132832088
90-91	2.1269594880153278E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	80.0
20-21	362.0
22-23	798.0
24-25	1574.0
26-27	2468.0
28-29	4741.0
30-31	11403.0
32-33	14388.0
34-35	21912.0
36-37	26296.0
38-39	31958.0
40-41	34650.0
42-43	40863.0
44-45	48585.0
46-47	46929.0
48-49	41784.0
50-51	36614.0
52-53	35627.0
54-55	38087.0
56-57	39608.0
58-59	39228.0
60-61	38523.0
62-63	38005.0
64-65	40152.0
66-67	42406.0
68-69	42044.0
70-71	41091.0
72-73	39666.0
74-75	40310.0
76-77	39875.0
78-79	43127.0
80-81	43327.0
82-83	42086.0
84-85	42771.0
86-87	45821.0
88-89	49766.0
90-91	52628.0
92-93	56958.0
94-95	70430.0
96-97	201838.0
98-99	369068.0
100-101	3963534.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.03885569995664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.68537882097338	83.4528544915744
2	5.499294577691816	9.90300381864695
3	1.1112551092320881	3.001684152779626
4	0.3601390858268908	1.2970604472272689
5	0.14452742031771218	0.6506541771336736
6	0.07249257869400223	0.3916289299408211
7	0.037159444569347215	0.23420557072289938
8	0.025554919041765614	0.18407485344204866
9	0.015177814461247467	0.12299337415052783
>10	0.04816472917341102	0.6601187689714038
>50	6.255421223713728E-4	0.04101899862976464
>100	2.0497307568483926E-4	0.03926368468156637
>500	2.4984820274547752E-5	0.021438732099020565
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	1.706082576785232E-5	0.0	0.0	0.0
4	0.0	3.412165153570464E-5	0.0	0.0	0.0
5	0.0	3.412165153570464E-5	0.0	1.706082576785232E-5	0.0
6	0.0	3.412165153570464E-5	0.0	1.706082576785232E-5	0.0
7	0.0	3.412165153570464E-5	0.0	1.706082576785232E-5	0.0
8	0.0	3.412165153570464E-5	0.0	3.412165153570464E-5	0.0
9	0.0	1.0236495460711392E-4	0.0	6.824330307140928E-5	0.0
10-11	0.0	1.0236495460711392E-4	0.0	1.279561932588924E-4	0.0
12-13	0.0	1.0236495460711392E-4	0.0	1.5354743191067088E-4	0.0
14-15	0.0	1.5354743191067088E-4	0.0	1.8766908344637552E-4	8.53041288392616E-6
16-17	0.0	1.5354743191067088E-4	0.0	2.2179073498208016E-4	3.412165153570464E-5
18-19	0.0	1.706082576785232E-4	0.0	2.3885156074993248E-4	5.118247730355696E-5
20-21	0.0	1.8766908344637552E-4	0.0	2.559123865177848E-4	5.118247730355696E-5
22-23	8.53041288392616E-6	2.3885156074993248E-4	0.0	2.64442799401711E-4	6.824330307140928E-5
24-25	1.706082576785232E-5	2.559123865177848E-4	0.0	2.9856445093741563E-4	8.53041288392616E-5
26-27	1.706082576785232E-5	2.559123865177848E-4	0.0	3.668077540088249E-4	8.53041288392616E-5
28-29	1.706082576785232E-5	2.559123865177848E-4	0.0	6.483113791783882E-4	8.53041288392616E-5
30-31	1.706082576785232E-5	2.8150362516956326E-4	0.0	0.0016122480350620444	8.53041288392616E-5
32-33	1.706082576785232E-5	2.9003403805348945E-4	0.0	0.003949581165257812	8.53041288392616E-5
34-35	2.559123865177848E-5	3.4974692824097254E-4	0.0	0.007139955583846196	8.53041288392616E-5
36-37	4.26520644196308E-5	4.094598184284557E-4	0.0	0.012317916204389375	8.53041288392616E-5
38-39	5.118247730355696E-5	4.094598184284557E-4	0.0	0.02228143845281513	8.53041288392616E-5
40-41	5.118247730355696E-5	4.6064229573201265E-4	0.0	0.030308556976589646	8.53041288392616E-5
42-43	5.118247730355696E-5	4.862335343837911E-4	0.0	0.036774609942605674	1.0236495460711392E-4
44-45	5.118247730355696E-5	5.88598488990905E-4	0.0	0.04443492071237137	1.1942578037496624E-4
46-47	5.118247730355696E-5	6.824330307140928E-4	0.0	0.05190756239869068	1.1942578037496624E-4
48-49	5.118247730355696E-5	6.994938564819451E-4	0.0	0.05920959582733147	1.3648660614281856E-4
50-51	5.118247730355696E-5	8.701021141604684E-4	0.0	0.06623865604368664	1.3648660614281856E-4
52-53	5.118247730355696E-5	8.871629399283207E-4	0.0	0.07290090850603295	1.3648660614281856E-4
54-55	5.118247730355696E-5	8.956933528122468E-4	0.0	0.07901721454380803	1.3648660614281856E-4
56-57	5.118247730355696E-5	9.212845914640253E-4	0.0	0.0846216958085475	1.3648660614281856E-4
58-59	5.118247730355696E-5	9.212845914640253E-4	0.0	0.089185466701448	1.3648660614281856E-4
60-61	5.118247730355696E-5	9.212845914640253E-4	0.0	0.09391131543914309	1.3648660614281856E-4
62-63	5.118247730355696E-5	9.298150043479514E-4	0.0	0.09888454615047204	1.3648660614281856E-4
64-65	5.118247730355696E-5	0.0010407103718389915	0.0	0.10371275984277425	1.3648660614281856E-4
66-67	5.118247730355696E-5	0.0010492407847229176	0.0	0.10863480807679965	1.3648660614281856E-4
68-69	5.118247730355696E-5	0.001117484087794327	0.0	0.11330947433719119	1.3648660614281856E-4
70-71	5.118247730355696E-5	0.0011345449135621794	0.0	0.11792442770739524	1.7913867056244936E-4
72-73	5.118247730355696E-5	0.0011430753264461055	0.0	0.12125981914501036	2.2179073498208016E-4
74-75	5.118247730355696E-5	0.0011516057393300316	0.0	0.12418575076419704	2.3885156074993248E-4
76-77	5.118247730355696E-5	0.0012027882166335884	0.0	0.12711168238338372	2.3885156074993248E-4
78-79	5.118247730355696E-5	0.001228379455285367	0.0	0.12877511289574933	2.3885156074993248E-4
80-81	5.118247730355696E-5	0.001228379455285367	0.0	0.12932958973320452	2.3885156074993248E-4
82-83	5.118247730355696E-5	0.0012369098681692932	0.0	0.1297475799645169	2.3885156074993248E-4
84-85	5.118247730355696E-5	0.0012625011068210716	0.0	0.12989259698354363	2.3885156074993248E-4
86-87	5.118247730355696E-5	0.001279561932588924	0.0	0.1299437794608472	2.3885156074993248E-4
88	5.118247730355696E-5	0.0013307444098924809	0.0	0.129969370699499	2.559123865177848E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7660	0.0	51.820873	1
GTATCAA	13785	0.0	37.499146	1
TCAACGC	16475	0.0	30.601387	4
ATCAACG	16500	0.0	30.529493	3
CAACGCA	17070	0.0	29.50653	5
TATCAAC	17300	0.0	29.363651	2
AACGCAG	17385	0.0	29.042831	6
ACGCAGA	20125	0.0	25.109814	7
CGCAGAG	20270	0.0	24.930193	8
GCAGAGT	24065	0.0	20.928051	9
TGGTATC	3210	0.0	20.808775	2
GTACATG	17235	0.0	20.735754	1
GAGTACT	14465	0.0	20.481682	12-13
TACATGG	17220	0.0	20.25968	2
GTGGTAT	3720	0.0	20.128971	1
ACATGGG	17430	0.0	19.527403	3
CAGAGTA	23295	0.0	19.236887	10-11
AGAGTAC	21785	0.0	18.383394	10-11
GTACTTT	16085	0.0	18.366451	14-15
CATGGGG	12290	0.0	17.447243	4
>>END_MODULE