FastQCFastQC Report
Fri 27 May 2016
SRR2139275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10252379
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144450.052.083331
GTATCAA264900.037.3782961
TCAACGC324200.029.7763234
ATCAACG324800.029.7081493
TATCAAC334250.029.1240182
CAACGCA332200.029.047375
AACGCAG339750.028.4018766
AGACCGA69350.025.22775588-89
CGAGACC69550.024.98670486-87
ACGCAGA390500.024.6889347
TGGTATC65150.024.5531712
GACCGAG70450.024.46198790-91
GAGACCG69400.024.43951488-89
ACCGAGG72500.024.40311890-91
GTGGTAT70300.024.3971731
ACGAGAC69750.024.3587486-87
CGCAGAG397950.024.2593368
CACGAGA74850.023.01589884-85
CCCACGA74700.022.9100282-83
TCCGAGC73750.022.4863776-77
Produced by FastQC (version 0.11.5)