FastQCFastQC Report
Fri 27 May 2016
SRR2139274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9668776
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA192500.058.8794941
GTATCAA375750.039.4682731
GTGGTAT73750.030.4401171
TCAACGC482000.030.409944
TGGTATC70500.030.4095332
ATCAACG485000.030.2478923
TATCAAC487800.030.1949922
CAACGCA494200.029.6686735
AACGCAG507050.028.9417116
ACGCAGA577000.025.3892947
CGCAGAG585700.025.0554378
GCAGAGT650350.022.4614479
GTACATG476750.021.9894981
TACATGG480600.021.4978432
GAGTACT383650.021.02440312-13
ACATGGG487450.020.8329373
ACCGAAC14750.020.560788
CAGAGTA638950.020.195510-11
GTACTTT410200.019.60270914-15
CATGGGG309200.019.2343964
Produced by FastQC (version 0.11.5)