##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139274_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9668776
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65168104008201	33.0	33.0	33.0	33.0	33.0
2	32.6417101813094	33.0	33.0	33.0	33.0	33.0
3	32.625650237424054	33.0	33.0	33.0	33.0	33.0
4	36.544402104258076	37.0	37.0	37.0	37.0	37.0
5	36.56546640443423	37.0	37.0	37.0	37.0	37.0
6	36.561290177784656	37.0	37.0	37.0	37.0	37.0
7	36.527422809257345	37.0	37.0	37.0	37.0	37.0
8	36.53022564593491	37.0	37.0	37.0	37.0	37.0
9	36.55977830079009	37.0	37.0	37.0	37.0	37.0
10-11	36.49521826754493	37.0	37.0	37.0	37.0	37.0
12-13	36.48975232232084	37.0	37.0	37.0	37.0	37.0
14-15	38.49180221984665	40.0	37.0	40.0	37.0	40.0
16-17	38.42826537712736	40.0	37.0	40.0	37.0	40.0
18-19	38.47248250450729	40.0	37.0	40.0	37.0	40.0
20-21	38.47992933567487	40.0	37.0	40.0	37.0	40.0
22-23	38.48733547465049	40.0	37.0	40.0	37.0	40.0
24-25	38.46063929807202	40.0	37.0	40.0	37.0	40.0
26-27	38.340114560568765	40.0	37.0	40.0	37.0	40.0
28-29	38.2907307684614	40.0	37.0	40.0	37.0	40.0
30-31	38.207994357407344	40.0	37.0	40.0	37.0	40.0
32-33	38.12527860975859	40.0	37.0	40.0	37.0	40.0
34-35	38.08032903988983	40.0	37.0	40.0	33.0	40.0
36-37	38.03314201746264	40.0	37.0	40.0	33.0	40.0
38-39	37.97251942566841	40.0	37.0	40.0	33.0	40.0
40-41	37.919393391928786	40.0	37.0	40.0	33.0	40.0
42-43	37.858303255788286	40.0	37.0	40.0	33.0	40.0
44-45	37.8079316009009	40.0	37.0	40.0	33.0	40.0
46-47	37.738683340265595	40.0	37.0	40.0	33.0	40.0
48-49	37.724132314928525	40.0	37.0	40.0	33.0	40.0
50-51	37.65502654225183	40.0	37.0	40.0	33.0	40.0
52-53	37.54233812393845	40.0	37.0	40.0	33.0	40.0
54-55	37.38948267152145	40.0	37.0	40.0	33.0	40.0
56-57	37.303017980257884	40.0	37.0	40.0	33.0	40.0
58-59	37.169189950456364	37.0	37.0	40.0	33.0	40.0
60-61	37.02291047633973	37.0	37.0	40.0	33.0	40.0
62-63	36.832118376281734	37.0	37.0	40.0	33.0	40.0
64-65	36.65350161018722	37.0	37.0	40.0	33.0	40.0
66-67	36.44798348914357	37.0	37.0	40.0	33.0	40.0
68-69	36.16000948903044	37.0	37.0	40.0	33.0	40.0
70-71	35.93269738038612	37.0	37.0	37.0	33.0	40.0
72-73	35.61208737254468	37.0	33.0	37.0	33.0	40.0
74-75	35.52598757550749	37.0	33.0	37.0	33.0	40.0
76-77	33.855426932823406	35.0	33.0	37.0	27.0	37.0
78-79	35.092723416811964	37.0	33.0	37.0	33.0	37.0
80-81	35.144816093460165	37.0	33.0	37.0	33.0	37.0
82-83	34.99214685287909	37.0	33.0	37.0	33.0	37.0
84-85	34.8629775134832	37.0	33.0	37.0	33.0	37.0
86-87	34.76726511052244	37.0	33.0	37.0	33.0	37.0
88-89	34.65341683164444	37.0	33.0	37.0	33.0	37.0
90-91	34.55912100016704	37.0	33.0	37.0	33.0	37.0
92-93	34.529048696848115	37.0	33.0	37.0	33.0	37.0
94-95	34.53572798105422	37.0	33.0	37.0	33.0	37.0
96-97	34.52255516340416	37.0	33.0	37.0	33.0	37.0
98-99	34.525597526927214	37.0	33.0	37.0	33.0	37.0
100	34.52059795687008	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	0.0
17	2.0
18	7.0
19	11.0
20	81.0
21	362.0
22	1257.0
23	3552.0
24	8387.0
25	16704.0
26	29104.0
27	46338.0
28	69902.0
29	99007.0
30	135444.0
31	183769.0
32	247282.0
33	336616.0
34	469765.0
35	686846.0
36	1213034.0
37	2879241.0
38	3148495.0
39	93567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.776800289922946	19.923514620671735	14.465419407792671	22.83426568161265
2	15.014506489756304	20.986968774537747	41.923714025436105	22.07481071026984
3	18.440110723425594	26.781931859834174	30.249682069374657	24.528275347365582
4	12.450676280017243	18.008473875079947	38.70661601840812	30.83423382649469
5	13.151168255423437	37.99164444392961	35.08917778217222	13.768009518474727
6	29.55474405446977	37.80994616071362	18.681237418262665	13.954072366553946
7	26.65677641099556	33.08721807186349	22.616264975008214	17.63974054213274
8	24.817257117136645	36.34306969155144	20.752781944684624	18.08689124662729
9	26.365285253021405	16.64375612733247	20.896707925633123	36.094250694013006
10-11	24.538663456556545	26.920016864868867	28.161243701854232	20.380075976720356
12-13	25.67277394978351	24.77127746468742	28.434680302477368	21.121268283051702
14-15	22.744890110339053	25.711921815789644	26.828607873299415	24.714580200571888
16-17	21.477853039516067	28.957564018444526	27.396952830430653	22.167630111608748
18-19	21.469299733492637	27.51661120290717	29.999743504245004	21.014345559355185
20-21	22.676040612386945	26.538994776762863	29.969836362772256	20.815128248077933
22-23	22.2967638910927	26.56204778063796	29.850910226097525	21.29027810217181
24-25	21.843655804934254	26.841261245852593	30.02507031188039	21.29001263733276
26-27	21.675822756649254	26.972834845338223	29.992672643462402	21.35866975455012
28-29	21.503604825824237	26.96448661865204	30.096297520818666	21.435611034705058
30-31	21.91202275046217	26.890014020406916	29.904640073221024	21.293323155909892
32-33	21.644811651367167	27.15370767346975	29.77690754558362	21.424573129579464
34-35	21.80744527997235	27.112274050555236	29.814843765001726	21.26543690447069
36-37	21.78897314859371	27.05662264047864	29.692279815668627	21.462124395259025
38-39	21.56944445250753	27.276440821956605	29.679937204362318	21.47417752117355
40-41	21.866815442834316	27.085053371642026	29.59003008229009	21.458101103233567
42-43	21.799903607831137	27.204139618377322	29.219842843331946	21.776113930459598
44-45	21.884872435513838	27.408003850471687	28.97149390509066	21.73562980892381
46-47	22.09966330808473	27.382507773357588	28.430106479421557	22.087722439136122
48-49	21.987267211939194	27.657160784397256	28.25542188509517	22.100150118568376
50-51	22.044355682942353	27.80739958907975	27.994303217292032	22.153941510685865
52-53	22.18368729707327	27.698505501490704	27.940896599714044	22.176910601721982
54-55	22.261063081085783	27.610381542386797	27.94099803908803	22.187557337439394
56-57	22.154778280288514	27.821010534361925	27.821917234358423	22.202293950991145
58-59	22.056984509128434	27.827561116365413	27.928292771266257	22.1871616032399
60-61	22.259092626673922	27.956573830078906	27.76366183027611	22.02067171297106
62-63	22.143606947880055	27.909804558905453	27.845744928470182	22.100843564744306
64-65	22.131652861893894	27.884570911802243	27.77992786870978	22.20384835759409
66-67	22.252126883417475	27.938510580220182	27.51357803526076	22.295784501101583
68-69	22.279377840022068	27.911841471067017	27.620131986189044	22.188648702721874
70-71	22.220869764441606	27.654407354974424	27.758234864945823	22.36648801563814
72-73	22.358336580560536	27.65111580543518	27.55501396941981	22.43553364458447
74-75	22.096755980908483	27.92532465280414	27.514556752290282	22.46336261399709
76-77	22.276786367957897	27.737319426891972	27.595523054233407	22.39037115091673
78-79	22.372683887802594	27.65176889588062	27.479796007035773	22.495751209281014
80-81	22.271478809291455	27.840599231503543	27.41796550811108	22.46995645109392
82-83	22.442074560132852	27.67609471303923	27.558606840835363	22.323223885992554
84-85	22.44295459705689	27.657701739215966	27.45310593492648	22.44623772880066
86-87	22.264795098879812	27.729167248706137	27.676605333764158	22.329432318649893
88-89	22.42666048787066	27.628943951656364	27.549969921565243	22.394425638907734
90-91	22.477430639618177	27.619129100762713	27.594447175482266	22.308993084136848
92-93	22.549662906794694	27.57214329898759	27.621776909609487	22.256416884608235
94-95	22.376831336039167	27.47951879635302	27.682825936153094	22.46082393145472
96-97	22.489943838546644	27.636247680099746	27.774424108743013	22.0993843726106
98-99	23.52825887449237	29.51676910573729	27.96931535402188	18.98565666574847
100	22.542800268018667	30.488099473381386	21.856567718065705	25.112532540534243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4358.0
1	3519.5
2	4223.0
3	6117.0
4	8006.5
5	12981.5
6	17598.5
7	21720.5
8	25740.5
9	26731.5
10	24298.0
11	20786.5
12	21088.0
13	26908.5
14	42440.5
15	64854.0
16	83391.5
17	93878.0
18	95141.5
19	87601.5
20	75067.0
21	61434.0
22	50412.0
23	45603.0
24	47693.0
25	56111.5
26	69824.0
27	85871.0
28	102066.5
29	120637.0
30	141025.5
31	162037.5
32	189260.5
33	219357.0
34	247418.5
35	273439.5
36	302971.5
37	329133.0
38	343668.0
39	354033.5
40	362476.0
41	367471.0
42	368382.0
43	374839.5
44	381568.0
45	384899.5
46	385862.0
47	384922.5
48	387081.0
49	385890.5
50	380363.5
51	370393.0
52	356164.0
53	340071.5
54	324142.0
55	304518.0
56	280783.0
57	254994.0
58	228543.5
59	200234.0
60	169056.5
61	139244.0
62	110436.5
63	84798.0
64	64256.5
65	48089.5
66	36073.0
67	27671.0
68	21187.0
69	14828.0
70	9761.0
71	6878.5
72	4822.5
73	3332.0
74	2269.5
75	1526.5
76	994.0
77	638.5
78	434.0
79	301.0
80	212.5
81	146.5
82	106.5
83	82.0
84	67.0
85	53.0
86	42.0
87	28.5
88	12.5
89	5.5
90	3.5
91	3.5
92	3.5
93	2.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	9.308313689343926E-5
10-11	0.0016186123248692493
12-13	2.0168012993578504E-4
14-15	0.0012721362042103365
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	226.0
20-21	670.0
22-23	1445.0
24-25	5357.0
26-27	5070.0
28-29	9472.0
30-31	24840.0
32-33	30306.0
34-35	41210.0
36-37	58939.0
38-39	70893.0
40-41	75803.0
42-43	90928.0
44-45	110886.0
46-47	104920.0
48-49	90937.0
50-51	76415.0
52-53	67461.0
54-55	70649.0
56-57	76797.0
58-59	76543.0
60-61	75425.0
62-63	74219.0
64-65	77604.0
66-67	83741.0
68-69	84755.0
70-71	83436.0
72-73	78961.0
74-75	79629.0
76-77	78638.0
78-79	85051.0
80-81	87994.0
82-83	84548.0
84-85	84573.0
86-87	89037.0
88-89	95075.0
90-91	99816.0
92-93	104914.0
94-95	120440.0
96-97	300345.0
98-99	563475.0
100-101	6147333.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98766228460359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.6639323983038	76.2032800577657
2	7.293787547217125	12.397639056770721
3	1.727370772149702	4.404156114712615
4	0.6178246603388028	2.100298943358964
5	0.2710727489386651	1.1518919620679202
6	0.13996977751407452	0.7137422508850358
7	0.08486594849417978	0.504879099906014
8	0.04796196463362119	0.3260940202230639
9	0.0370273401539461	0.2832180372270641
>10	0.11297284157349799	1.514873685487975
>50	0.0023826291079598193	0.13815927994339416
>100	6.53091583954719E-4	0.09030324130513614
>500	5.942842696393996E-5	0.035842610797741425
>1k	1.1885156373271892E-4	0.1356216395487028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0342570765937694E-5
2	0.0	2.0685141531875388E-5	0.0	2.0685141531875388E-5	1.0342570765937694E-5
3	0.0	2.0685141531875388E-5	0.0	2.0685141531875388E-5	1.0342570765937694E-5
4	0.0	6.205542459562617E-5	0.0	3.1027712297813085E-5	1.0342570765937694E-5
5	1.0342570765937694E-5	6.205542459562617E-5	0.0	3.1027712297813085E-5	1.0342570765937694E-5
6	1.0342570765937694E-5	6.205542459562617E-5	0.0	3.1027712297813085E-5	2.0685141531875388E-5
7	1.0342570765937694E-5	7.239799536156386E-5	0.0	5.171285382968847E-5	2.0685141531875388E-5
8	1.0342570765937694E-5	7.239799536156386E-5	0.0	5.171285382968847E-5	2.0685141531875388E-5
9	1.0342570765937694E-5	1.5513856148906543E-4	0.0	8.274056612750155E-5	3.1027712297813085E-5
10-11	1.0342570765937694E-5	1.5513856148906543E-4	0.0	1.2928213457422116E-4	3.1027712297813085E-5
12-13	1.0342570765937694E-5	1.5513856148906543E-4	0.0	1.551385614890654E-4	3.1027712297813085E-5
14-15	1.0342570765937694E-5	2.068514153187539E-4	0.0	1.8099498840390966E-4	3.619899768078193E-5
16-17	1.5513856148906543E-5	2.171939860846916E-4	0.0	2.2753655685062927E-4	4.1370283063750775E-5
18-19	2.0685141531875388E-5	2.3270784223359815E-4	0.0	7.601789512964206E-4	4.1370283063750775E-5
20-21	2.0685141531875388E-5	2.585642691484424E-4	0.0	9.722016519981434E-4	4.1370283063750775E-5
22-23	4.1370283063750775E-5	2.740781252973489E-4	0.0	0.001623783610252218	5.688413921265732E-5
24-25	4.1370283063750775E-5	3.1027712297813085E-4	0.0	0.003516474060418816	6.205542459562617E-5
26-27	4.1370283063750775E-5	3.361335498929751E-4	0.0	0.008279227898133124	6.205542459562617E-5
28-29	4.1370283063750775E-5	3.619899768078193E-4	0.0	0.030174450209623224	6.722670997859502E-5
30-31	4.1370283063750775E-5	4.2921668678641437E-4	0.0	0.04282341425636503	7.75692807445327E-5
32-33	4.1370283063750775E-5	4.343879721693832E-4	0.0	0.04966502481803281	8.274056612750155E-5
34-35	4.1370283063750775E-5	5.222998236798535E-4	0.0	0.057240957904082165	8.274056612750155E-5
36-37	4.1370283063750775E-5	5.791839628925109E-4	0.0	0.06856090160740097	8.274056612750155E-5
38-39	4.1370283063750775E-5	5.998691044243863E-4	0.0	0.09519819261507351	9.308313689343926E-5
40-41	4.1370283063750775E-5	6.722670997859502E-4	0.0	0.1229731664069992	9.308313689343926E-5
42-43	4.1370283063750775E-5	6.981235267007945E-4	0.0	0.1403693704353064	9.308313689343926E-5
44-45	4.1370283063750775E-5	7.860353782112649E-4	0.0	0.1609148872618416	9.308313689343926E-5
46-47	4.1370283063750775E-5	8.429195174239222E-4	0.0	0.18074159542014417	9.308313689343926E-5
48-49	4.1370283063750775E-5	8.532620881898597E-4	0.0	0.2000460037547669	1.0342570765937695E-4
50-51	4.1370283063750775E-5	9.360026543173614E-4	0.0	0.21676994068328814	1.0342570765937695E-4
52-53	5.171285382968847E-5	9.515165104662679E-4	0.0	0.2561027373061492	1.0859699304234578E-4
54-55	5.171285382968847E-5	9.566877958492367E-4	0.0	0.29890029513559935	1.1376827842531464E-4
56-57	5.171285382968847E-5	9.82544222764081E-4	0.0	0.3423494349233036	1.1893956380828349E-4
58-59	5.171285382968847E-5	9.82544222764081E-4	0.0	0.37469582499377374	1.2411084919125234E-4
60-61	5.171285382968847E-5	9.877155081470497E-4	0.0	0.3997920729573216	1.2411084919125234E-4
62-63	5.171285382968847E-5	0.0010032293642959565	0.0	0.41765886395547897	1.3445341995719004E-4
64-65	5.171285382968847E-5	0.0010652847888915826	0.0	0.4230163156122347	1.3445341995719004E-4
66-67	5.171285382968847E-5	0.0010704560742745514	0.0	0.42798074957988474	1.3445341995719004E-4
68-69	6.205542459562617E-5	0.001085969930423458	0.0	0.4331882339605344	1.3445341995719004E-4
70-71	6.205542459562617E-5	0.0011118263573383022	0.0	0.43789927494441905	1.3445341995719004E-4
72-73	6.205542459562617E-5	0.0011273402134872087	0.0	0.44168465584475225	1.3445341995719004E-4
74-75	6.205542459562617E-5	0.001179053067316897	0.0	0.444539205376151	1.3445341995719004E-4
76-77	6.205542459562617E-5	0.001220423350380648	0.0	0.4471351906384014	1.3445341995719004E-4
78-79	7.239799536156386E-5	0.0012411084919125234	0.0	0.4490071959470361	1.3445341995719004E-4
80-81	7.239799536156386E-5	0.0012411084919125234	0.0	0.44958120862454565	1.3962470534015887E-4
82-83	7.239799536156386E-5	0.0012411084919125234	0.0	0.45001559659671503	1.5513856148906543E-4
84-85	7.239799536156386E-5	0.0012411084919125234	0.0	0.4502586470097146	1.5513856148906543E-4
86-87	7.239799536156386E-5	0.0012514510626784612	0.0	0.4503672440027569	1.5513856148906543E-4
88	7.239799536156386E-5	0.001354876770337838	0.0	0.45037758657352284	1.5513856148906543E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	19250	0.0	58.879494	1
GTATCAA	37575	0.0	39.468273	1
GTGGTAT	7375	0.0	30.440117	1
TCAACGC	48200	0.0	30.40994	4
TGGTATC	7050	0.0	30.409533	2
ATCAACG	48500	0.0	30.247892	3
TATCAAC	48780	0.0	30.194992	2
CAACGCA	49420	0.0	29.668673	5
AACGCAG	50705	0.0	28.941711	6
ACGCAGA	57700	0.0	25.389294	7
CGCAGAG	58570	0.0	25.055437	8
GCAGAGT	65035	0.0	22.461447	9
GTACATG	47675	0.0	21.989498	1
TACATGG	48060	0.0	21.497843	2
GAGTACT	38365	0.0	21.024403	12-13
ACATGGG	48745	0.0	20.832937	3
ACCGAAC	1475	0.0	20.56078	8
CAGAGTA	63895	0.0	20.1955	10-11
GTACTTT	41020	0.0	19.602709	14-15
CATGGGG	30920	0.0	19.234396	4
>>END_MODULE