FastQCFastQC Report
Fri 27 May 2016
SRR2139273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10471469
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA267600.055.5369221
GTATCAA505800.038.4570161
GTGGTAT94200.034.6477971
TGGTATC95100.033.629182
TATCAAC653100.029.6514232
ATCAACG659550.029.212063
TCAACGC665300.028.9660364
CAACGCA678750.028.3745825
AACGCAG697400.027.6511046
ACGCAGA792050.024.3161627
CGCAGAG801300.024.0362648
GTACATG691000.022.0956861
TACATGG692550.021.8091132
GCAGAGT880150.021.785079
ACATGGG708750.020.8761833
GAGTACT496700.020.74327512-13
CAGAGTA873950.019.40965710-11
GTACTTT542950.019.0291914-15
CATGGGG430100.018.8712524
CTTATAC163800.018.5972771
Produced by FastQC (version 0.11.5)