##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139273_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10471469
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61833139170827	33.0	33.0	33.0	33.0	33.0
2	32.608647936598004	33.0	33.0	33.0	33.0	33.0
3	32.588268083494306	33.0	33.0	33.0	33.0	33.0
4	36.49851716125025	37.0	37.0	37.0	37.0	37.0
5	36.52586136672897	37.0	37.0	37.0	37.0	37.0
6	36.528938585407644	37.0	37.0	37.0	37.0	37.0
7	36.4939363330971	37.0	37.0	37.0	37.0	37.0
8	36.4994113051378	37.0	37.0	37.0	37.0	37.0
9	36.526774610133494	37.0	37.0	37.0	37.0	37.0
10-11	36.46124106369412	37.0	37.0	37.0	37.0	37.0
12-13	36.45275710599917	37.0	37.0	37.0	37.0	37.0
14-15	38.384766167955995	40.0	37.0	40.0	37.0	40.0
16-17	38.315326770293645	40.0	37.0	40.0	37.0	40.0
18-19	38.372788908604896	40.0	37.0	40.0	37.0	40.0
20-21	38.39666405231518	40.0	37.0	40.0	37.0	40.0
22-23	38.40515020895256	40.0	37.0	40.0	37.0	40.0
24-25	38.373495144667956	40.0	37.0	40.0	37.0	40.0
26-27	38.24022277736363	40.0	37.0	40.0	37.0	40.0
28-29	38.17587751268036	40.0	37.0	40.0	37.0	40.0
30-31	38.08339581832322	40.0	37.0	40.0	35.0	40.0
32-33	37.99322176779353	40.0	37.0	40.0	33.0	40.0
34-35	37.93976220292974	40.0	37.0	40.0	33.0	40.0
36-37	37.88701830389611	40.0	37.0	40.0	33.0	40.0
38-39	37.817928944266285	40.0	37.0	40.0	33.0	40.0
40-41	37.778962317644705	40.0	37.0	40.0	33.0	40.0
42-43	37.71812118069276	40.0	37.0	40.0	33.0	40.0
44-45	37.66175362411864	40.0	37.0	40.0	33.0	40.0
46-47	37.58839940076393	40.0	37.0	40.0	33.0	40.0
48-49	37.57305846954611	40.0	37.0	40.0	33.0	40.0
50-51	37.49070990911177	40.0	37.0	40.0	33.0	40.0
52-53	37.38770077564058	40.0	37.0	40.0	33.0	40.0
54-55	37.24255091160729	40.0	37.0	40.0	33.0	40.0
56-57	37.16234779758861	37.0	37.0	40.0	33.0	40.0
58-59	37.038877980745895	37.0	37.0	40.0	33.0	40.0
60-61	36.906442403293056	37.0	37.0	40.0	33.0	40.0
62-63	36.73601866873081	37.0	37.0	40.0	33.0	40.0
64-65	36.58746508133104	37.0	37.0	40.0	33.0	40.0
66-67	36.40523764908362	37.0	37.0	40.0	33.0	40.0
68-69	36.14416129203781	37.0	37.0	40.0	33.0	40.0
70-71	35.929767760434885	37.0	37.0	38.5	33.0	40.0
72-73	35.62191608545665	37.0	33.0	37.0	33.0	40.0
74-75	35.545310039225285	37.0	33.0	37.0	33.0	40.0
76-77	33.8578927536214	35.0	33.0	37.0	27.0	37.0
78-79	35.1014877255927	37.0	33.0	37.0	33.0	37.0
80-81	35.14313827168236	37.0	33.0	37.0	33.0	37.0
82-83	34.99193296351511	37.0	33.0	37.0	33.0	37.0
84-85	34.86571449553146	37.0	33.0	37.0	33.0	37.0
86-87	34.76076193164887	37.0	33.0	37.0	33.0	37.0
88-89	34.64749593147933	37.0	33.0	37.0	33.0	37.0
90-91	34.548898004504366	37.0	33.0	37.0	33.0	37.0
92-93	34.51879798026996	37.0	33.0	37.0	33.0	37.0
94-95	34.52592648639201	37.0	33.0	37.0	33.0	37.0
96-97	34.51672674993176	37.0	33.0	37.0	33.0	37.0
98-99	34.53485808229865	37.0	33.0	37.0	33.0	37.0
100	34.5306890778755	37.0	33.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	3.0
18	10.0
19	23.0
20	98.0
21	484.0
22	1674.0
23	4445.0
24	10338.0
25	20005.0
26	34017.0
27	54258.0
28	79962.0
29	113352.0
30	155444.0
31	209167.0
32	279564.0
33	377489.0
34	524521.0
35	767806.0
36	1350040.0
37	3060147.0
38	3322093.0
39	106528.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05111345886618	20.21241718807552	14.556906963101357	22.17956238995694
2	14.75205627787276	21.173963271055857	42.46769961311063	21.60628083796075
3	18.470292945526552	27.68321235540114	30.430372281100198	23.41612241797211
4	12.20234715874153	18.476729482749747	38.43343278770151	30.88749057080721
5	12.736847141504215	38.64763387066323	34.73923286217053	13.876286125662023
6	28.469138379724946	37.918872700668835	19.042466725537743	14.569522194068474
7	25.922676178480785	33.96140503304741	22.40832685461801	17.707591933853788
8	24.867303718322614	36.102699630777686	20.886009403265195	18.143987247634502
9	26.459623129665005	16.629793401164097	20.878086355351066	36.03249711381983
10-11	24.39511365630992	27.17834814492099	27.697538396834165	20.728999801934926
12-13	25.798342311397622	25.078317726450056	28.383080353357055	20.74025960879527
14-15	22.83416133222039	25.93290317787219	26.974981832537896	24.25795365736952
16-17	21.060230422302737	29.474508304422237	27.45466753518537	22.01059373808966
18-19	21.04134100000678	27.57618821198821	30.539568994569912	20.842901793435097
20-21	22.586914677570384	26.36633210916389	30.459218731272763	20.58753448199296
22-23	22.28988049495235	26.553082334719956	30.30060215751301	20.856435012814686
24-25	21.640537817569903	26.896997723274556	30.53435096271317	20.928113496442368
26-27	21.491761974117132	27.03738284971547	30.51760670309871	20.953248473068687
28-29	21.106745953062322	26.976844129423572	30.620013454162542	21.296396463351563
30-31	21.561665935149545	27.015070977281002	30.51761040040095	20.905652687168498
32-33	21.49021344254012	27.030919534800148	30.547809918945973	20.931057103713755
34-35	21.161209754275028	27.155848715438296	30.732327129800506	20.95061440048617
36-37	21.359149725686112	27.25680244145932	30.293702960666774	21.090344872187792
38-39	21.170725019833075	27.239802894470433	30.356663889269974	21.232808196426518
40-41	21.353484377181093	27.143923435730837	30.27482280220682	21.227769384881253
42-43	21.447784707162	27.249065046737563	29.966893237534997	21.336257008565433
44-45	21.447930719387635	27.572699957254716	29.472704894261888	21.50666442909576
46-47	21.83115902595354	27.586414118160008	28.707687940071185	21.87473891581527
48-49	21.78957287023939	27.825944293333638	28.440388126880613	21.94409470954636
50-51	21.6822627385485	28.04353673962398	28.317818936969424	21.956381584858097
52-53	21.754884394661993	28.067184056992602	28.334335548426527	21.84359599991888
54-55	21.75496460767575	28.033059816287654	28.15048871198684	22.06148686404976
56-57	21.955772312811302	28.19594514972711	27.851567616722427	21.996714920739162
58-59	21.625298256198217	28.3131573144925	28.09678259750448	21.964761831804807
60-61	22.028768186689998	28.426294003231963	27.924570057513225	21.620367752564817
62-63	21.620147158179147	28.515971606655537	27.89963467505208	21.964246560113228
64-65	21.639322845801917	28.437804486398257	27.901167826021666	22.021704841778163
66-67	21.772369754720152	28.31458639690128	28.021398414588848	21.89164543378972
68-69	21.829597950673268	28.422399891060728	27.883381351798292	21.86462080646771
70-71	21.746681972273304	28.22362330015773	27.72878598916814	22.30090873840082
72-73	21.833689778651287	28.16671110266535	27.714439194561223	22.28515992412214
74-75	21.782890303288955	28.2946936092637	27.89949960829482	22.022916479152528
76-77	21.90616663778177	27.931906210793038	27.788554932026045	22.373372219399148
78-79	21.923616021761553	27.89840782174442	27.55783689284611	22.620139263647918
80-81	22.126630751519542	28.18324888713185	27.550777955749332	22.139342405599276
82-83	22.23875025339893	27.77675875150306	27.710876626456976	22.273614368641027
84-85	22.039097548806524	27.731182397373015	27.66775373150181	22.561966322318654
86-87	21.913912745036434	27.988072353021476	27.768673931752474	22.329340970189616
88-89	22.31724276168167	27.881931646917373	27.67852246233798	22.122303129062978
90-91	22.384993780885218	27.78464953034797	27.737177439501696	22.093179249265113
92-93	22.073644792231605	27.427266857777433	27.804984375403528	22.694103974587435
94-95	22.262777841923658	27.74495822140228	27.891736615471242	22.10052732120282
96-97	22.63340778549755	27.56912430431633	27.924131131783913	21.873336778402212
98-99	23.193263953026918	29.65284421691758	28.28496077683269	18.868931053222813
100	22.026415923422768	30.648983797670446	22.224496057224677	25.100104221682106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3586.0
1	3322.5
2	5219.5
3	7824.5
4	10787.5
5	18742.0
6	25621.5
7	30535.5
8	34176.0
9	33761.0
10	30027.0
11	25385.5
12	26124.5
13	35013.0
14	58145.5
15	91606.0
16	120082.0
17	137268.5
18	140013.5
19	128793.5
20	110299.0
21	89511.5
22	72568.5
23	64957.0
24	67525.5
25	78542.0
26	94853.0
27	113885.0
28	134633.0
29	158404.0
30	182750.5
31	206501.0
32	231175.0
33	254248.5
34	277047.5
35	298668.5
36	319041.5
37	337544.5
38	351125.0
39	361336.0
40	370310.0
41	377574.5
42	383736.5
43	391983.5
44	401984.0
45	409202.5
46	411072.5
47	412606.0
48	415215.5
49	419646.5
50	425249.5
51	420980.5
52	401079.0
53	382217.5
54	365846.5
55	339157.5
56	308290.0
57	275623.5
58	242263.0
59	211328.0
60	180143.5
61	147386.0
62	115148.5
63	88299.0
64	66676.5
65	49436.5
66	37063.0
67	27712.0
68	20420.5
69	13955.0
70	9237.5
71	6318.0
72	4165.5
73	2748.5
74	1878.0
75	1304.5
76	856.5
77	538.0
78	375.0
79	261.0
80	183.0
81	122.0
82	81.5
83	60.0
84	45.5
85	31.5
86	23.0
87	17.5
88	12.5
89	8.0
90	3.5
91	2.0
92	2.5
93	3.5
94	3.0
95	2.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.527961358621221E-4
10-11	0.0015566106340953692
12-13	2.0054492831903529E-4
14-15	0.0013226415510564945
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	265.0
20-21	851.0
22-23	1767.0
24-25	5322.0
26-27	6345.0
28-29	12661.0
30-31	30931.0
32-33	37953.0
34-35	52648.0
36-37	74401.0
38-39	90635.0
40-41	101338.0
42-43	124715.0
44-45	152143.0
46-47	141992.0
48-49	122053.0
50-51	100181.0
52-53	88760.0
54-55	96571.0
56-57	105536.0
58-59	67927.0
60-61	111779.0
62-63	107310.0
64-65	112666.0
66-67	119853.0
68-69	111510.0
70-71	110893.0
72-73	102445.0
74-75	102123.0
76-77	102280.0
78-79	109617.0
80-81	112823.0
82-83	106858.0
84-85	104750.0
86-87	109312.0
88-89	117631.0
90-91	123538.0
92-93	124946.0
94-95	135012.0
96-97	357886.0
98-99	565108.0
100-101	6108134.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.68721710951594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.96071128817553	72.7322643139282
2	8.215668676946779	13.586615591810958
3	2.104557213583632	5.220599377169631
4	0.793332392312214	2.6239379105252687
5	0.36898004621259	1.5254966595129829
6	0.1943186441017764	0.9640600747962207
7	0.10865426748573938	0.6289023303828126
8	0.07201173152142155	0.4763559742995166
9	0.045161580451439184	0.336085686701732
>10	0.13339555222802296	1.6744048598210743
>50	0.002696949536384884	0.14232759322750677
>100	4.717161229658093E-4	0.05892873522084683
>500	1.9970837475496748E-5	0.010766690991753074
>1k	1.997048392715288E-5	0.019254201611508426
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	9.549758491382632E-6	0.0	0.0	0.0	0.0
3	9.549758491382632E-6	0.0	0.0	9.549758491382632E-6	0.0
4	9.549758491382632E-6	4.7748792456913164E-5	0.0	1.9099516982765264E-5	0.0
5	9.549758491382632E-6	4.7748792456913164E-5	0.0	1.9099516982765264E-5	0.0
6	9.549758491382632E-6	4.7748792456913164E-5	0.0	3.819903396553053E-5	0.0
7	9.549758491382632E-6	4.7748792456913164E-5	0.0	3.819903396553053E-5	0.0
8	9.549758491382632E-6	6.684830943967842E-5	0.0	3.819903396553053E-5	0.0
9	9.549758491382632E-6	1.2414686038797422E-4	0.0	6.684830943967842E-5	0.0
10-11	1.9099516982765264E-5	1.2414686038797422E-4	0.0	1.0982222265090027E-4	0.0
12-13	1.9099516982765264E-5	1.2414686038797422E-4	0.0	1.5757101510781342E-4	0.0
14-15	1.9099516982765264E-5	2.1009468681041792E-4	0.0	1.7189565284488737E-4	2.3874396228456582E-5
16-17	2.8649275474147897E-5	2.1009468681041792E-4	0.0	2.1964444530180055E-4	4.297391321122185E-5
18-19	2.8649275474147897E-5	2.2441932454749186E-4	0.0	2.960425132328616E-4	6.684830943967842E-5
20-21	2.8649275474147897E-5	2.387439622845658E-4	0.0	4.67938166077749E-4	6.684830943967842E-5
22-23	2.8649275474147897E-5	2.9126763398717027E-4	0.0	0.0010600231925434723	7.162318868536974E-5
24-25	2.8649275474147897E-5	3.008173924785529E-4	0.0	0.0027742048417466547	7.639806793106105E-5
26-27	2.8649275474147897E-5	3.055922717242442E-4	0.0	0.0066418570307566205	8.594782642244368E-5
28-29	3.342415471983921E-5	3.055922717242442E-4	0.0	0.02657220300227217	8.594782642244368E-5
30-31	4.7748792456913164E-5	3.342415471983921E-4	0.0	0.03781704362587522	8.594782642244368E-5
32-33	4.7748792456913164E-5	3.342415471983921E-4	0.0	0.04180406779602747	8.594782642244368E-5
34-35	4.7748792456913164E-5	3.6289082267254005E-4	0.0	0.04709940887949914	8.594782642244368E-5
36-37	4.7748792456913164E-5	3.9154009814668794E-4	0.0	0.05556049490286415	8.594782642244368E-5
38-39	5.7298550948295794E-5	4.392888906036011E-4	0.0	0.08016067277666582	8.594782642244368E-5
40-41	6.684830943967842E-5	4.8703768306051424E-4	0.0	0.10656098012609311	8.594782642244368E-5
42-43	6.684830943967842E-5	4.965874415518969E-4	0.0	0.12089039274241274	1.0027246415951764E-4
44-45	6.684830943967842E-5	5.586608717458839E-4	0.0	0.13985621310629864	1.1459710189659159E-4
46-47	6.684830943967842E-5	6.111845434484884E-4	0.0	0.15769038708895572	1.193719811422829E-4
48-49	6.684830943967842E-5	6.111845434484884E-4	0.0	0.1756630325697378	1.527961358621221E-4
50-51	6.684830943967842E-5	6.828077321338582E-4	0.0	0.19390784616752435	1.527961358621221E-4
52-53	6.684830943967842E-5	6.875826113795495E-4	0.0	0.24024804924695858	1.527961358621221E-4
54-55	6.684830943967842E-5	6.923574906252407E-4	0.0	0.2934879528364167	1.527961358621221E-4
56-57	6.684830943967842E-5	6.971323698709321E-4	0.0	0.3469952496636336	1.527961358621221E-4
58-59	6.684830943967842E-5	6.971323698709321E-4	0.0	0.5791021297966885	1.527961358621221E-4
60-61	6.684830943967842E-5	7.11457007608006E-4	0.0	0.7852766407463938	1.6234589435350474E-4
62-63	6.684830943967842E-5	7.210067660993887E-4	0.0	0.7930787934338535	1.7189565284488737E-4
64-65	6.684830943967842E-5	7.830801962933759E-4	0.0	0.7992670369362694	1.7189565284488737E-4
66-67	6.684830943967842E-5	7.974048340304498E-4	0.0	0.8050541905820473	1.7189565284488737E-4
68-69	6.684830943967842E-5	8.16504351013215E-4	0.0	0.810936841812739	1.7189565284488737E-4
70-71	6.684830943967842E-5	8.30828988750289E-4	0.0	0.8166332727528487	1.7189565284488737E-4
72-73	6.684830943967842E-5	8.30828988750289E-4	0.0	0.8209784128664278	1.7189565284488737E-4
74-75	6.684830943967842E-5	8.499285057330543E-4	0.0	0.824516598387485	1.7189565284488737E-4
76-77	7.162318868536974E-5	8.642531434701283E-4	0.0	0.8276298196556757	1.7189565284488737E-4
78-79	7.639806793106105E-5	8.690280227158196E-4	0.0	0.8296209443011291	1.7189565284488737E-4
80-81	7.639806793106105E-5	8.690280227158196E-4	0.0	0.830380150101194	1.7189565284488737E-4
82-83	7.639806793106105E-5	8.690280227158196E-4	0.0	0.8308671877842545	1.7189565284488737E-4
84-85	7.639806793106105E-5	8.690280227158196E-4	0.0	0.8310963819880477	1.7189565284488737E-4
86-87	8.117294717675237E-5	8.738029019615108E-4	0.0	0.8311966544522073	1.7189565284488737E-4
88	8.594782642244368E-5	9.263265736641153E-4	0.0	0.8312014293314529	1.7189565284488737E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	26760	0.0	55.536922	1
GTATCAA	50580	0.0	38.457016	1
GTGGTAT	9420	0.0	34.647797	1
TGGTATC	9510	0.0	33.62918	2
TATCAAC	65310	0.0	29.651423	2
ATCAACG	65955	0.0	29.21206	3
TCAACGC	66530	0.0	28.966036	4
CAACGCA	67875	0.0	28.374582	5
AACGCAG	69740	0.0	27.651104	6
ACGCAGA	79205	0.0	24.316162	7
CGCAGAG	80130	0.0	24.036264	8
GTACATG	69100	0.0	22.095686	1
TACATGG	69255	0.0	21.809113	2
GCAGAGT	88015	0.0	21.78507	9
ACATGGG	70875	0.0	20.876183	3
GAGTACT	49670	0.0	20.743275	12-13
CAGAGTA	87395	0.0	19.409657	10-11
GTACTTT	54295	0.0	19.02919	14-15
CATGGGG	43010	0.0	18.871252	4
CTTATAC	16380	0.0	18.597277	1
>>END_MODULE