FastQCFastQC Report
Fri 27 May 2016
SRR2139272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9841098
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125550.049.5117031
GTATCAA230200.037.21691
ATCTCGT84200.035.6913294
TATCTCG105750.031.02911494
TCAACGC273100.030.8623874
ATCAACG273900.030.677223
CAACGCA278550.030.1817535
AACGCAG285850.029.5627356
TATCAAC288900.029.3697132
ACGCAGA330350.025.5279437
CGCAGAG333300.025.3153978
GTGGTAT66500.022.63531
GCCGTCT50450.022.37546590-91
TGGTATC62500.021.5853332
GCAGAGT390000.021.546499
TATGCCG48500.021.45491486-87
GTACATG290800.021.450771
TACATGG284000.021.246492
TGCCGTC51000.021.12488-89
CGCTATC159950.021.11690994
Produced by FastQC (version 0.11.5)