FastQCFastQC Report
Fri 27 May 2016
SRR2139271_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139271_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9535367
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.037.4209941
GTATCAA131950.033.4232561
TCAACGC141350.029.8267564
ATCAACG141750.029.68173
CAACGCA145450.028.9859895
AACGCAG154350.027.3705396
TATCAAC160200.027.0983432
TGTAGAT138650.023.69405694
ACGCAGA184250.022.8821377
CGCAGAG187050.022.447338
GGTGTAG156350.020.71988194
GTACATG320700.019.9952621
GTGTAGA145600.019.6083794
TACATGG320250.019.2687762
AGGTGTA191050.018.96952694
ACATGGG325100.018.5432853
GCAGAGT242800.017.0798599
TAGGTGT218600.016.69808494
CATGGGG226650.016.6974874
AGAGTAC203850.016.31065810-11
Produced by FastQC (version 0.11.5)