##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139271_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9535367
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.916327184889685	33.0	31.0	34.0	30.0	34.0
2	31.91106351753425	33.0	31.0	34.0	30.0	34.0
3	32.03801615606405	34.0	31.0	34.0	30.0	34.0
4	35.52462826024421	37.0	35.0	37.0	33.0	37.0
5	35.54384513988816	37.0	35.0	37.0	33.0	37.0
6	35.595905747518685	37.0	35.0	37.0	33.0	37.0
7	35.50036144387521	37.0	35.0	37.0	33.0	37.0
8	35.48345333745413	37.0	35.0	37.0	33.0	37.0
9	37.09400414268271	39.0	37.0	39.0	33.0	39.0
10-11	37.07859215067443	39.0	37.0	39.0	33.0	39.0
12-13	37.04407696106505	39.0	37.0	39.0	33.0	39.0
14-15	38.412312971278396	40.0	38.0	41.0	33.0	41.0
16-17	38.425568675017956	40.0	38.0	41.0	33.0	41.0
18-19	38.437442313442155	40.0	38.0	41.0	33.0	41.0
20-21	38.49396128389972	40.0	38.0	41.0	33.0	41.0
22-23	38.4794623741692	40.0	38.0	41.0	33.0	41.0
24-25	38.486211377660084	40.0	38.0	41.0	33.0	41.0
26-27	38.462991089555004	40.0	38.0	41.0	33.0	41.0
28-29	38.435356238769	40.0	38.0	41.0	33.0	41.0
30-31	38.38873049086498	40.0	38.0	41.0	33.0	41.0
32-33	38.346181970481126	40.0	38.0	41.0	33.0	41.0
34-35	38.28957036035388	40.0	38.0	41.0	33.0	41.0
36-37	38.23635999016667	40.0	38.0	41.0	33.0	41.0
38-39	38.15774184358153	40.0	38.0	41.0	33.0	41.0
40-41	38.090979976660634	40.0	38.0	41.0	33.0	41.0
42-43	38.005112108495524	40.0	37.5	41.0	32.5	41.0
44-45	37.886790843893145	40.0	37.0	41.0	32.0	41.0
46-47	37.78604184404497	40.0	37.0	41.0	32.0	41.0
48-49	37.68545213379221	40.0	37.0	41.0	32.0	41.0
50-51	37.550766524730946	40.0	36.5	41.0	31.0	41.0
52-53	37.42688794506483	40.0	36.0	41.0	31.0	41.0
54-55	37.28623417181231	39.0	36.0	41.0	31.0	41.0
56-57	37.108621400676796	39.0	35.5	41.0	31.0	41.0
58-59	37.259712349028405	39.0	35.5	41.0	31.0	41.0
60-61	37.32509556898124	39.0	35.5	41.0	31.0	41.0
62-63	37.17015180469708	39.0	35.0	41.0	31.0	41.0
64-65	36.96859660419285	39.0	35.0	41.0	31.0	41.0
66-67	36.707135093618	38.0	35.0	40.0	31.0	41.0
68-69	36.44047681607681	37.5	35.0	40.0	31.0	41.0
70-71	36.20406563837955	37.0	35.0	40.0	31.0	41.0
72-73	35.97045512124936	36.5	35.0	39.0	31.0	41.0
74-75	35.681162242290156	36.0	34.0	39.0	31.0	41.0
76-77	35.41194610341806	36.0	34.0	39.0	30.5	40.5
78-79	35.084070703755756	35.0	34.0	37.5	30.0	39.5
80-81	34.77509383889758	35.0	34.0	37.0	30.0	39.0
82-83	34.485405550922664	35.0	34.0	36.5	30.0	39.0
84-85	34.24132548879922	35.0	34.0	36.0	30.0	37.0
86-87	34.04943778685394	35.0	34.0	36.0	30.0	37.0
88-89	33.929958056561134	35.0	34.0	35.5	30.0	37.0
90-91	33.81702675849732	35.0	34.0	35.0	30.0	36.0
92-93	33.718795050200704	35.0	34.0	35.0	30.0	36.0
94-95	33.674631418501065	35.0	34.0	35.0	30.0	36.0
96-97	33.66737920694851	35.0	34.0	35.0	30.0	36.0
98-99	33.694971520542275	35.0	34.0	35.0	30.0	36.0
100	33.34004656811932	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	55.0
17	359.0
18	1275.0
19	3810.0
20	9610.0
21	19487.0
22	31804.0
23	45470.0
24	60621.0
25	75749.0
26	89190.0
27	97717.0
28	112137.0
29	138534.0
30	166083.0
31	193309.0
32	243420.0
33	316095.0
34	428695.0
35	599861.0
36	924953.0
37	1698560.0
38	2601382.0
39	1588991.0
40	88198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09942490939258	20.6515386350625	14.262156873458567	23.986879582086353
2	13.996241570985154	21.228674260780945	42.18854921892362	22.586534949310288
3	17.766636564696462	26.610753419349248	30.477872534953292	25.144737481000995
4	12.21345754180201	18.23841704257424	39.741794940876424	29.806330474747327
5	13.187264140653971	38.351935703630616	35.606832852595566	12.853967303119845
6	29.2762617317194	38.16182429055955	19.623104176273447	12.9388098014476
7	26.190580813512476	32.881807276007315	23.652807490262305	17.274804420217908
8	24.02405696602973	36.99640506757632	21.733175031438222	17.246362934955727
9	25.352637187430748	17.579375812173772	21.646623564672446	35.42136343572303
10-11	23.671211135011028	27.199796379069056	29.41196643170102	19.7170260542189
12-13	24.391752279999842	24.851037388457964	29.256507028442808	21.50070330309938
14-15	21.81427654765829	26.352634306466804	26.566627152362432	25.26646199351247
16-17	21.64528205641275	29.384301298681688	27.2147979279654	21.755618716940166
18-19	21.724038644512923	29.033362283735226	28.281595698329998	20.961003373421853
20-21	22.7259511864899	28.160750217765695	27.862593257332	21.2507053384124
22-23	22.450532756464835	28.06432342711121	27.683152423257134	21.801991393166816
24-25	22.087862595283514	28.221138749141545	27.759188961951942	21.931809693623
26-27	22.0362501202572	28.346322863377832	27.65619636068854	21.961230655676427
28-29	21.979626867297693	28.398772062657056	27.745404050605888	21.87619701943936
30-31	22.238824360524912	28.192327137250118	27.677853584640964	21.890994917584003
32-33	22.04687613230389	28.338150137014225	27.646966074398115	21.968007656283774
34-35	22.18897422198619	28.285480956787367	27.704843088644783	21.82070173258166
36-37	22.104797521236947	28.15255682739371	27.743857814338014	21.998787837031326
38-39	21.98115870690951	28.205497054344207	27.876647654494498	21.936696584251784
40-41	22.177808911647418	28.054101649593278	27.833126147467112	21.93496329129219
42-43	22.026732450274046	28.10686475468151	27.7683686332095	22.09803416183494
44-45	22.094753893237826	28.127641613396715	27.754384078958033	22.02322041440743
46-47	22.039586186248293	28.077966417201704	27.714684479356457	22.167762917193542
48-49	21.935327749405722	28.06553115156233	27.852673326492415	22.14646777253953
50-51	21.811208539500253	28.05350772823214	27.933796824969086	22.201486907298516
52-53	21.982498417091566	27.836332853582867	27.90381591523386	22.277352814091707
54-55	22.00292282747503	27.630957311312006	28.020911928214115	22.345207932998854
56-57	21.80875575520428	27.513557873899448	28.144332970086612	22.53335340080966
58-59	21.686587077063432	27.453300498090233	28.3503994324152	22.509712992431137
60-61	21.624212412868427	27.315008826214882	28.689383398261647	22.37139536265504
62-63	21.492757459279886	27.526466774709057	28.698629657861257	22.2821461081498
64-65	21.310597453131233	27.849879876229387	28.35850205558404	22.481020615055343
66-67	21.164885191421245	28.074885854517774	28.080142317882956	22.68008663617803
68-69	21.283342946154026	28.27253195187685	28.086180390450227	22.3579447115189
70-71	21.35829688875374	28.317463312737235	27.735899059936347	22.588340738572676
72-73	21.683501331627657	28.17839559115219	27.286651587633166	22.85145148958699
74-75	21.931072462484575	27.975332114195716	27.346028373300925	22.747567050018784
76-77	22.141761415317585	27.507471887801245	27.45794945715494	22.892817239726234
78-79	22.499520019026207	27.314240000058902	27.255384423189273	22.930855557725618
80-81	22.863758061925974	27.314995651164658	26.97083964006572	22.850406646843645
82-83	22.55315674538786	27.633355338786238	26.81756335587368	22.995924559952226
84-85	22.97673719818876	27.621454858700023	26.765181940821247	22.636626002289965
86-87	22.825448953279395	27.924808985916492	26.822727392138145	22.427014668665965
88-89	22.713045499651113	28.14249033928315	26.698897537503726	22.445566623562012
90-91	22.88277054870757	28.22618779457357	27.10962392595102	21.781417730767842
92-93	23.083031170456778	27.95685768506043	27.37953055550429	21.580580588978506
94-95	22.728767950536525	28.001698206640874	27.85045934907625	21.419074493746354
96-97	23.013499223357268	27.688217500934876	28.088916796420666	21.20936647928719
98-99	23.67833898834641	29.776121612846595	28.575130089473998	17.970409309332993
100	22.87414980823446	30.96784693272685	22.47183793063574	23.686165328402954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3090.0
1	2309.5
2	1674.5
3	1815.0
4	1787.5
5	1867.5
6	2144.5
7	2487.5
8	2789.0
9	3110.5
10	3474.5
11	3846.0
12	4299.0
13	4964.5
14	5868.5
15	6908.0
16	8127.5
17	9662.0
18	11671.0
19	14400.5
20	18293.0
21	23515.0
22	30257.0
23	39078.5
24	51170.5
25	66238.5
26	84864.0
27	106342.5
28	129040.0
29	152943.5
30	174942.0
31	195444.5
32	217226.5
33	238060.5
34	256825.5
35	274695.0
36	294567.0
37	315013.5
38	332097.5
39	345130.5
40	358027.5
41	369456.0
42	377703.5
43	386748.0
44	394597.5
45	401013.0
46	403500.0
47	402786.5
48	400377.5
49	392532.5
50	382494.0
51	371019.5
52	355248.5
53	336065.0
54	314989.5
55	290958.5
56	265989.5
57	239865.5
58	212366.5
59	184601.0
60	154260.5
61	125116.5
62	99556.5
63	77922.0
64	59827.0
65	45188.0
66	34443.5
67	26891.5
68	21164.5
69	16166.5
70	12358.5
71	9587.0
72	7314.5
73	5561.5
74	4214.5
75	3160.5
76	2380.5
77	1805.0
78	1391.5
79	1050.0
80	788.0
81	600.0
82	469.5
83	332.0
84	233.0
85	181.0
86	153.0
87	123.0
88	80.5
89	54.5
90	43.0
91	37.5
92	31.5
93	18.5
94	9.5
95	7.0
96	6.0
97	5.0
98	4.5
99	6.0
100	20.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.4120728651555833E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.573090999014511E-5
12-13	4.7192729970435326E-5
14-15	1.573090999014511E-5
16-17	5.243636663381703E-5
18-19	9.805600560523786E-4
20-21	4.511627355528512E-4
22-23	0.0
24-25	8.929907631661901E-5
26-27	1.1565706460687743E-4
28-29	0.0
30-31	0.0
32-33	3.171440962701476E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.8973814996979254E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.1600974203936596E-4
76-77	1.819785214390716E-5
78-79	4.6019057043750504E-4
80-81	1.8629878039503413E-5
82-83	4.399831549306398E-5
84-85	4.452446692604925E-4
86-87	1.4165387596554825E-4
88-89	4.5657082049363784E-5
90-91	1.984049695418611E-5
92-93	7.047154862234834E-4
94-95	4.773285313173886E-4
96-97	4.1108628645412156E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2994.0
20-21	5774.0
22-23	6398.0
24-25	7183.0
26-27	9680.0
28-29	14185.0
30-31	23455.0
32-33	27477.0
34-35	35836.0
36-37	51444.0
38-39	59023.0
40-41	54629.0
42-43	57819.0
44-45	66653.0
46-47	71683.0
48-49	43122.0
50-51	42511.0
52-53	14362.0
54-55	14330.0
56-57	14013.0
58-59	14174.0
60-61	15604.0
62-63	80610.0
64-65	85940.0
66-67	90478.0
68-69	92053.0
70-71	91551.0
72-73	87581.0
74-75	89257.0
76-77	92442.0
78-79	96957.0
80-81	97540.0
82-83	94421.0
84-85	94865.0
86-87	97984.0
88-89	104174.0
90-91	109510.0
92-93	114082.0
94-95	134661.0
96-97	325490.0
98-99	599109.0
100-101	6304313.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.92242132397064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.78394281398663	72.79257091500743
2	8.394459301841035	13.921777820283737
3	2.148269513162889	5.344191290638032
4	0.7995373314102302	2.65198285837769
5	0.36448757239323215	1.5112096022671424
6	0.1904860814172608	0.9477340259780564
7	0.10810440250855322	0.6274995168253252
8	0.06073994253599274	0.40293624849306847
9	0.03863025523120985	0.2882982870121414
>10	0.10930610673763357	1.3746882646288268
>50	0.0016361197646669599	0.0908740828030085
>100	4.005590107060971E-4	0.046237087685458846
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.0487273326763407E-5	0.0	0.0	0.0
5	0.0	2.0974546653526813E-5	0.0	1.0487273326763407E-5	0.0
6	0.0	2.0974546653526813E-5	0.0	1.0487273326763407E-5	0.0
7	0.0	2.0974546653526813E-5	0.0	1.0487273326763407E-5	0.0
8	0.0	4.1949093307053626E-5	0.0	1.0487273326763407E-5	0.0
9	0.0	1.0487273326763407E-4	0.0	2.0974546653526813E-5	0.0
10-11	5.243636663381703E-6	1.0487273326763407E-4	0.0	4.7192729970435326E-5	0.0
12-13	1.0487273326763407E-5	1.0487273326763407E-4	0.0	6.816727662396214E-5	0.0
14-15	1.0487273326763407E-5	1.2584727992116088E-4	0.0	7.865454995072555E-5	0.0
16-17	1.0487273326763407E-5	1.2584727992116088E-4	0.0	8.389818661410725E-5	0.0
18-19	1.0487273326763407E-5	1.3109091658454258E-4	0.0	1.1011636993101577E-4	0.0
20-21	1.0487273326763407E-5	1.3633455324792428E-4	0.0	1.4157818991130598E-4	0.0
22-23	1.0487273326763407E-5	1.3633455324792428E-4	0.0	2.5693819650570343E-4	0.0
24-25	1.0487273326763407E-5	1.5206546323806938E-4	0.0	4.1424729640715454E-4	0.0
26-27	1.0487273326763407E-5	1.573090999014511E-4	0.0	9.123927794284163E-4	0.0
28-29	1.0487273326763407E-5	1.573090999014511E-4	0.0	0.004215883877358889	0.0
30-31	2.0974546653526813E-5	1.782836465549779E-4	0.0	0.015222277233797083	0.0
32-33	2.0974546653526813E-5	1.782836465549779E-4	0.0	0.04750210453357485	0.0
34-35	2.0974546653526813E-5	1.782836465549779E-4	0.0	0.09036883425672027	0.0
36-37	2.0974546653526813E-5	1.9401455654512303E-4	0.0	0.1489612303333474	0.0
38-39	2.0974546653526813E-5	2.3072001318879496E-4	0.0	0.21526177230514568	0.0
40-41	2.0974546653526813E-5	2.7266910649584856E-4	0.0	0.277414597676209	0.0
42-43	2.0974546653526813E-5	2.9364365314937536E-4	0.0	0.3894658695360126	0.0
44-45	2.0974546653526813E-5	3.093745631395205E-4	0.0	0.49223066086496725	0.0
46-47	2.0974546653526813E-5	3.198618364662839E-4	0.0	0.6025043398958845	0.0
48-49	2.0974546653526813E-5	3.3034910979304734E-4	0.0	0.6738125548812122	0.0
50-51	2.0974546653526813E-5	3.460800197831924E-4	0.0	1.0447264379021806	0.0
52-53	2.0974546653526813E-5	3.460800197831924E-4	0.0	1.543076422753314	0.0
54-55	2.0974546653526813E-5	3.460800197831924E-4	0.0	2.206968017067408	0.0
56-57	2.0974546653526813E-5	3.460800197831924E-4	0.0	2.9292212874449404	0.0
58-59	2.0974546653526813E-5	3.5132365644657414E-4	0.0	3.673707577275211	0.0
60-61	2.0974546653526813E-5	3.7229820310010094E-4	0.0	4.413867866858192	0.0
62-63	2.0974546653526813E-5	3.775418397634826E-4	0.0	4.953464297703486	0.0
64-65	2.0974546653526813E-5	3.775418397634826E-4	0.0	4.961481818161797	0.0
66-67	2.0974546653526813E-5	3.88029113090246E-4	0.0	4.965110414732857	0.0
68-69	2.0974546653526813E-5	3.88029113090246E-4	0.0	4.968775716760561	0.0
70-71	2.0974546653526813E-5	3.9327274975362774E-4	0.0	4.972435775151601	0.0
72-73	2.0974546653526813E-5	3.985163864170094E-4	0.0	4.975723535339542	0.0
74-75	2.0974546653526813E-5	3.985163864170094E-4	0.0	4.978612779141065	0.0
76-77	2.0974546653526813E-5	4.0900365974377286E-4	0.0	4.981108750192835	0.0
78-79	2.0974546653526813E-5	4.1949093307053626E-4	0.0	4.983090844851594	0.0
80-81	2.0974546653526813E-5	4.24734569733918E-4	0.0	4.983788248527823	0.0
82-83	2.0974546653526813E-5	4.3522184306068134E-4	0.0	4.98428115037418	0.0
84-85	2.0974546653526813E-5	4.4046547972406306E-4	0.0	4.984422728564092	0.0
86-87	2.0974546653526813E-5	4.5095275305082646E-4	0.0	4.984464677657399	0.0
88	2.0974546653526813E-5	4.6144002637758986E-4	0.0	4.984464677657399	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7830	0.0	37.420994	1
GTATCAA	13195	0.0	33.423256	1
TCAACGC	14135	0.0	29.826756	4
ATCAACG	14175	0.0	29.6817	3
CAACGCA	14545	0.0	28.985989	5
AACGCAG	15435	0.0	27.370539	6
TATCAAC	16020	0.0	27.098343	2
TGTAGAT	13865	0.0	23.694056	94
ACGCAGA	18425	0.0	22.882137	7
CGCAGAG	18705	0.0	22.44733	8
GGTGTAG	15635	0.0	20.719881	94
GTACATG	32070	0.0	19.995262	1
GTGTAGA	14560	0.0	19.60837	94
TACATGG	32025	0.0	19.268776	2
AGGTGTA	19105	0.0	18.969526	94
ACATGGG	32510	0.0	18.543285	3
GCAGAGT	24280	0.0	17.079859	9
TAGGTGT	21860	0.0	16.698084	94
CATGGGG	22665	0.0	16.697487	4
AGAGTAC	20385	0.0	16.310658	10-11
>>END_MODULE