FastQCFastQC Report
Fri 27 May 2016
SRR2139270_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139270_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27170867
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA218800.039.1389771
GTATCAA349900.032.1126481
TCAACGC382050.027.8457074
ATCAACG382600.027.8170013
CAACGCA392600.027.1195015
AACGCAG411150.026.0855756
TATCAAC426550.025.4281352
ACGCAGA499000.021.3455857
CGCAGAG506000.021.024618
GTACATG848300.020.4198271
TACATGG836900.020.1493452
ACATGGG856550.019.090373
CATGGGG645150.017.8734
GCAGAGT657150.016.0701279
ACCGAAC33550.016.0095568
AGAGTAC548250.015.68705310-11
TATACCG26250.015.0162755
CAGAGTA634750.014.74012510-11
GTACTAG68000.014.6510591
GTGGTAT136700.014.4809751
Produced by FastQC (version 0.11.5)