##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139270_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27170867
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70361685550925	33.0	31.0	34.0	30.0	34.0
2	31.71299097669574	33.0	31.0	34.0	28.0	34.0
3	31.852831637650723	34.0	31.0	34.0	30.0	34.0
4	35.34927968989727	37.0	35.0	37.0	33.0	37.0
5	35.36634116239279	37.0	35.0	37.0	33.0	37.0
6	35.42337905522117	37.0	35.0	37.0	33.0	37.0
7	35.311864063815115	37.0	35.0	37.0	32.0	37.0
8	35.30264934129632	37.0	35.0	37.0	32.0	37.0
9	36.86916836330619	39.0	37.0	39.0	33.0	39.0
10-11	36.875910768692066	39.0	37.0	39.0	33.0	39.0
12-13	36.846550387957805	39.0	37.0	39.0	33.0	39.0
14-15	38.180833353606275	40.0	38.0	41.0	33.0	41.0
16-17	38.18815166994855	40.0	38.0	41.0	33.0	41.0
18-19	38.188745099668694	40.0	38.0	41.0	33.0	41.0
20-21	38.25890124267292	40.0	38.0	41.0	33.0	41.0
22-23	38.23928559748781	40.0	38.0	41.0	33.0	41.0
24-25	38.2488236980178	40.0	38.0	41.0	33.0	41.0
26-27	38.23163971762625	40.0	38.0	41.0	33.0	41.0
28-29	38.20653323593152	40.0	38.0	41.0	33.0	41.0
30-31	38.15538503053546	40.0	38.0	41.0	33.0	41.0
32-33	38.116407285799724	40.0	38.0	41.0	32.0	41.0
34-35	38.05615522424434	40.0	38.0	41.0	32.0	41.0
36-37	37.993265776271016	40.0	37.5	41.0	32.0	41.0
38-39	37.9062534534087	40.0	37.0	41.0	32.0	41.0
40-41	37.835389843239454	40.0	37.0	41.0	32.0	41.0
42-43	37.733195426365235	40.0	37.0	41.0	31.5	41.0
44-45	37.58700588763879	40.0	37.0	41.0	31.0	41.0
46-47	37.46952858279708	40.0	36.0	41.0	31.0	41.0
48-49	37.3525421119157	40.0	36.0	41.0	31.0	41.0
50-51	37.206192740068204	39.0	36.0	41.0	31.0	41.0
52-53	37.07580186376377	39.0	35.5	41.0	30.0	41.0
54-55	36.92735465603536	39.0	35.0	40.0	30.0	41.0
56-57	36.74158250347631	39.0	35.0	40.0	30.0	41.0
58-59	36.90608994571811	39.0	35.0	40.0	30.5	41.0
60-61	36.98472075323605	39.0	35.0	41.0	31.0	41.0
62-63	36.827364221594024	38.5	35.0	40.0	31.0	41.0
64-65	36.61959734701587	38.0	35.0	40.0	31.0	41.0
66-67	36.35153942401101	37.5	35.0	40.0	30.0	41.0
68-69	36.07510702318682	37.0	34.0	40.0	30.0	41.0
70-71	35.84277847157095	37.0	34.0	39.5	30.0	41.0
72-73	35.611551003919914	36.0	34.0	39.0	30.0	41.0
74-75	35.34743847764305	36.0	34.0	39.0	30.0	40.5
76-77	35.109775319290094	35.5	34.0	38.5	30.0	40.0
78-79	34.795754614691354	35.0	34.0	37.0	30.0	39.0
80-81	34.509957110196275	35.0	34.0	37.0	30.0	39.0
82-83	34.24012090811681	35.0	34.0	36.0	30.0	39.0
84-85	34.0232641737064	35.0	34.0	36.0	29.5	37.0
86-87	33.80601423325381	35.0	33.0	36.0	29.0	37.0
88-89	33.65471632190432	35.0	33.0	35.0	29.0	36.5
90-91	33.53559797556433	35.0	33.0	35.0	29.0	36.0
92-93	33.44486746060021	35.0	33.0	35.0	29.0	36.0
94-95	33.41540534631852	35.0	33.0	35.0	29.0	36.0
96-97	33.42747301545624	35.0	33.0	35.0	29.0	36.0
98-99	33.484452659171886	35.0	33.0	35.0	29.5	35.0
100	33.11038569128226	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	261.0
17	1317.0
18	4335.0
19	13943.0
20	34767.0
21	68923.0
22	111657.0
23	156994.0
24	208804.0
25	257111.0
26	293998.0
27	313149.0
28	358576.0
29	433693.0
30	514072.0
31	607838.0
32	766599.0
33	994751.0
34	1343853.0
35	1877050.0
36	2894557.0
37	5087052.0
38	6952176.0
39	3694300.0
40	181084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.08685600647193	20.487943943783613	14.403684652388899	24.021515397355557
2	14.121319720861319	20.939670419791902	41.97603999901807	22.962969860328712
3	17.834186152396242	26.146258049108262	30.460930819763682	25.558624978731814
4	12.415054698107351	18.051727977616615	39.26940572047259	30.263811603803443
5	13.345496135523613	38.15814258398218	35.50475580017417	12.991605480320029
6	29.817484293011336	37.83186602032243	19.235893355924198	13.114756330742042
7	26.478402509452682	32.7673152842208	23.296505282404517	17.457776923922
8	24.242639156219752	36.91177896206915	21.3939555699108	17.451626311800293
9	25.267784057093213	17.683631516064615	21.445532084051642	35.603052342790534
10-11	23.813462202776225	27.111168149345893	29.170251082980155	19.90511856489773
12-13	24.505355705260175	24.721206611294065	28.98661977227202	21.786817911173735
14-15	21.886010302434677	26.233327772228325	26.334914549444317	25.54574737589268
16-17	21.806437076448564	29.265138028101006	27.01127723525997	21.917147660190462
18-19	21.89601304644502	28.908561895164702	28.040883866470473	21.1545411919198
20-21	22.810201180549157	28.004407565184803	27.72484994567876	21.460541308587285
22-23	22.574752560517453	27.8500250923819	27.541929410696437	22.03329293640421
24-25	22.300192031569495	28.019239743523688	27.587320848877862	22.093247376028955
26-27	22.20233050737957	28.1646303762637	27.508694787985537	22.12434432837119
28-29	22.121928265397468	28.19140833769591	27.571959578728052	22.114703818178572
30-31	22.441447790672857	27.967328686366383	27.49474573582191	22.09647778713885
32-33	22.22187538399119	28.097891844896537	27.457875152147455	22.222357618964818
34-35	22.354157926497088	28.01714092827418	27.57018331775508	22.058517827473647
36-37	22.283594032159975	27.828909492109133	27.661284301915106	22.22621217381579
38-39	22.11519029560339	27.8720041441986	27.821959232554345	22.190846327643666
40-41	22.306664198587722	27.651938446311714	27.842923759769178	22.198473595331382
42-43	22.071851611498822	27.742021997652376	27.769499770383334	22.416626620465465
44-45	22.15907374088889	27.751686591241832	27.790315347361382	22.298924320507897
46-47	22.02422700726506	27.70885835630215	27.77832658679434	22.48858804963845
48-49	21.886221882958136	27.763678585279	27.892491958062273	22.45760757370059
50-51	21.75706920921264	27.805774620508792	27.965388996308178	22.47176717397038
52-53	21.919756254808668	27.644461075891165	27.901222350125863	22.5345603191743
54-55	21.925242091552033	27.482652584137927	27.903231853280776	22.68887347102926
56-57	21.847969240190398	27.33184348129067	27.993737941582385	22.826449336936548
58-59	21.755992604891652	27.289245224400748	28.0972614671086	22.857500703599
60-61	21.739723320258186	27.074627676178203	28.4304244795616	22.75522452400201
62-63	21.68717573137861	27.238819357833282	28.347497844962984	22.72650706582513
64-65	21.60747473658695	27.525107412760764	27.998584571190534	22.868833279461757
66-67	21.4344504679604	27.75743045034604	27.756308097342338	23.051810984351228
68-69	21.502504440704193	27.91796648530156	27.740439061819917	22.839090012174335
70-71	21.543985205795313	27.961156045965158	27.44167953709403	23.053179211145505
72-73	21.767530420697145	27.818877849180897	27.11557367722164	23.29801805290032
74-75	21.993780437545286	27.685164708068115	27.21154061824628	23.10951423614032
76-77	22.165735645476122	27.267400209157078	27.3674352442844	23.1994289010824
78-79	22.444545133055847	27.038760294155406	27.330853075307726	23.18584149748102
80-81	22.77284759117026	27.04543668543218	26.95363626879586	23.228079454601698
82-83	22.5267504701322	27.079634490374062	27.131930975569134	23.261684063924605
84-85	22.637652659502077	27.271776674934767	27.064592845109743	23.025977820453413
86-87	22.401533541379166	27.55459511343277	27.197987327319034	22.845884017869032
88-89	22.14486163926223	27.678947597817512	27.230442447408393	22.945748315511867
90-91	21.99073955004878	27.813904686515716	27.527656304569113	22.667699458866387
92-93	21.933951499562234	27.4553377393191	27.997893467362566	22.6128172937561
94-95	21.58590852538007	27.449951953885382	28.368872950265462	22.595266570469086
96-97	22.021218084415423	27.312937121020152	28.27874733887077	22.387097455693652
98-99	23.344807463711188	29.305866565840784	28.58956903431613	18.7597569361319
100	22.540501702539753	30.49651682234232	22.31380122173912	24.64918025337881
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12468.0
1	8988.5
2	6103.5
3	6647.5
4	6399.0
5	6422.5
6	7200.5
7	8289.0
8	9174.5
9	10238.5
10	11688.0
11	12926.5
12	14266.5
13	16089.0
14	18145.5
15	20709.0
16	23931.0
17	28032.0
18	33222.5
19	40119.5
20	49957.5
21	63366.5
22	81893.0
23	105020.0
24	134764.0
25	174654.0
26	223672.0
27	279120.5
28	337078.0
29	401297.5
30	462134.0
31	518759.0
32	579321.0
33	633044.0
34	686298.0
35	741506.0
36	802250.5
37	863251.0
38	907176.5
39	942212.0
40	984424.5
41	1023954.0
42	1050268.0
43	1077261.0
44	1104809.0
45	1128074.5
46	1140510.5
47	1145778.5
48	1144915.0
49	1126724.5
50	1103177.0
51	1072263.0
52	1023034.5
53	967487.5
54	911755.5
55	845940.5
56	771887.0
57	693785.5
58	618165.5
59	544640.5
60	461417.5
61	378208.0
62	304019.5
63	238977.0
64	182170.0
65	137056.5
66	105560.0
67	84032.0
68	67445.0
69	52394.0
70	40500.0
71	31551.0
72	24406.0
73	18543.5
74	14108.0
75	10908.5
76	8326.5
77	6297.5
78	4887.0
79	3834.0
80	2935.5
81	2232.5
82	1772.5
83	1282.5
84	911.0
85	703.0
86	567.0
87	446.0
88	284.0
89	160.5
90	112.0
91	94.5
92	78.0
93	42.5
94	19.0
95	18.0
96	15.5
97	11.5
98	10.0
99	17.5
100	68.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.6561856491366286E-4
6	0.0
7	7.360825107273905E-6
8	3.6804125536369523E-6
9	0.0
10-11	3.3123712982732574E-5
12-13	1.0673196405547162E-4
14-15	2.944330042909562E-5
16-17	8.46494887336499E-5
18-19	9.090619007483272E-4
20-21	4.5478471449057517E-4
22-23	0.0
24-25	9.22039835366468E-5
26-27	1.3844008919307316E-4
28-29	0.0
30-31	0.0
32-33	6.12067091901206E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	4.2392191358351795E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.5619586511641506E-4
76-77	1.2644254879433764E-5
78-79	4.1832862475297E-4
80-81	4.5424313705674845E-5
82-83	3.7279432945615536E-5
84-85	3.7352714411751113E-4
86-87	8.120557068410324E-5
88-89	1.6032434990193874E-5
90-91	2.560256450182088E-5
92-93	5.184395575348004E-4
94-95	3.3052780368520644E-4
96-97	3.166491631420275E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	10399.0
20-21	19430.0
22-23	21467.0
24-25	24706.0
26-27	31529.0
28-29	40828.0
30-31	51813.0
32-33	56246.0
34-35	69063.0
36-37	101837.0
38-39	119024.0
40-41	103215.0
42-43	110947.0
44-45	134517.0
46-47	174260.0
48-49	109266.0
50-51	112374.0
52-53	47657.0
54-55	49529.0
56-57	49422.0
58-59	49572.0
60-61	54394.0
62-63	232016.0
64-65	250236.0
66-67	264476.0
68-69	270091.0
70-71	268927.0
72-73	268102.0
74-75	276704.0
76-77	295072.0
78-79	309139.0
80-81	315276.0
82-83	312487.0
84-85	318906.0
86-87	331347.0
88-89	345158.0
90-91	356950.0
92-93	386957.0
94-95	448209.0
96-97	991038.0
98-99	1782231.0
100-101	1.760605E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.41623737589461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.64001805928086	66.29927966953188
2	8.906233331265486	13.789741473967071
3	2.7285435218589043	6.337007189360653
4	1.1027316567020453	3.4147734278863626
5	0.5899480041697782	2.2835777365121412
6	0.331356840162673	1.5391439876495918
7	0.1941331261046504	1.0520339321129466
8	0.12008915166602525	0.7437480217317456
9	0.0864252639380081	0.6021646873469263
>10	0.2938381999993237	3.3960718320728605
>50	0.0050854468563860935	0.26025392704108186
>100	0.001512331779676685	0.18611790442885748
>500	5.324539909059301E-5	0.031954695096267
>1k	3.182081698162018E-5	0.0641315152616476
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.360825107273905E-6	0.0
2	0.0	3.3123712982732574E-5	0.0	1.472165021454781E-5	7.360825107273905E-6
3	0.0	3.3123712982732574E-5	0.0	1.472165021454781E-5	7.360825107273905E-6
4	0.0	1.2145361427001943E-4	0.0	4.048453809000648E-5	1.1041237660910857E-5
5	0.0	1.3617526448456724E-4	0.0	7.360825107273905E-5	1.1041237660910857E-5
6	0.0	1.3617526448456724E-4	0.0	8.096907618001296E-5	1.472165021454781E-5
7	0.0	1.3985567703820418E-4	0.0	8.46494887336499E-5	1.472165021454781E-5
8	0.0	1.7297939002093677E-4	0.0	8.46494887336499E-5	1.472165021454781E-5
9	0.0	3.385979549345996E-4	0.0	9.569072639456077E-5	1.840206276818476E-5
10-11	0.0	3.385979549345996E-4	0.0	1.2145361427001943E-4	1.840206276818476E-5
12-13	0.0	3.385979549345996E-4	0.0	1.3801547076138572E-4	1.840206276818476E-5
14-15	0.0	4.858144570800778E-4	0.0	1.6745877119048134E-4	4.232474436682496E-5
16-17	0.0	5.005361072946255E-4	0.0	1.9322165906594E-4	5.520618830455429E-5
18-19	0.0	5.465412642150874E-4	0.0	2.465876410936758E-4	6.0726807135009715E-5
20-21	0.0	6.091082776269156E-4	0.0	3.44118573765055E-4	6.992783851910209E-5
22-23	0.0	6.753557035923807E-4	0.0	6.679948784851068E-4	8.832990128728686E-5
24-25	0.0	7.397629232810274E-4	0.0	0.0013470309946311246	9.937113894819771E-5
26-27	0.0	7.618453986028491E-4	0.0	0.0033454950112559896	1.0305155150183466E-4
28-29	0.0	7.69206223710123E-4	0.0	0.015417248187185192	1.0305155150183466E-4
30-31	0.0	8.869794254265055E-4	0.0	0.053855476897369525	1.0489175777865314E-4
32-33	0.0	8.998608693642348E-4	0.0	0.154415389100392	1.1777320171638247E-4
34-35	0.0	0.0010378763401256205	0.0	0.2940355197351634	1.324948519309303E-4
36-37	0.0	0.0011758918108870063	0.0	0.49439901936143593	1.3617526448456724E-4
38-39	1.8402062768184762E-6	0.0012310979991915607	0.0	0.7250523143041405	1.4169588331502267E-4
40-41	3.6804125536369523E-6	0.0013801547076138572	0.0	0.9474835676020202	1.4353608959184115E-4
42-43	3.6804125536369523E-6	0.0014114382143197712	0.0	1.2724952795948692	1.5273712097593355E-4
44-45	3.6804125536369523E-6	0.0015880980168943449	0.0	1.5655573302095953	1.6745877119048134E-4
46-47	3.6804125536369523E-6	0.0017334743127630046	0.0	1.8568822260990052	1.7297939002093677E-4
48-49	3.6804125536369523E-6	0.0017408351378702786	0.0	2.033389291552603	1.8402062768184762E-4
50-51	3.6804125536369523E-6	0.001985582572687136	0.0	2.4365637651533167	1.8770104023548456E-4
52-53	3.6804125536369523E-6	0.002002144429178502	0.0	2.9373298982325444	2.024226904500324E-4
54-55	3.6804125536369523E-6	0.002024226904500324	0.0	3.573680589581481	2.153041343877617E-4
56-57	3.6804125536369523E-6	0.002068391855143967	0.0	4.265535582651816	2.1714434066458018E-4
58-59	3.6804125536369523E-6	0.002075752680251241	0.0	4.973455576518777	2.2082475321821713E-4
60-61	3.6804125536369523E-6	0.0021272784560021586	0.0	5.670273605917691	2.3002578460230952E-4
62-63	3.6804125536369523E-6	0.002189845469413987	0.0	6.175544563962571	2.5026805364731277E-4
64-65	3.6804125536369523E-6	0.002460355792106303	0.0	6.185717224260824	2.5394846620094974E-4
66-67	3.6804125536369523E-6	0.0024916392988122166	0.0	6.189051678034419	2.594690850314051E-4
68-69	3.6804125536369523E-6	0.0025302836306254047	0.0	6.192203951386608	2.6682991013867905E-4
70-71	3.6804125536369523E-6	0.0025799692000995035	0.0	6.195061791734508	2.8339176663004533E-4
72-73	3.6804125536369523E-6	0.00259653105659087	0.0	6.1977208898045095	3.2387630472005183E-4
74-75	3.6804125536369523E-6	0.002635175388404058	0.0	6.2001702043589555	3.2387630472005183E-4
76-77	3.6804125536369523E-6	0.0027308661147986187	0.0	6.202385812716245	3.2387630472005183E-4
78-79	3.6804125536369523E-6	0.002775031065442262	0.0	6.203887421038129	3.2387630472005183E-4
80-81	3.6804125536369523E-6	0.0027805516842727175	0.0	6.204724714894081	3.330773361041442E-4
82-83	3.6804125536369523E-6	0.002786072303103173	0.0	6.205223410795099	3.533196051491474E-4
84-85	3.6804125536369523E-6	0.0028118351909786317	0.0	6.205403751010227	3.533196051491474E-4
86-87	3.6804125536369523E-6	0.0028725619981136413	0.0	6.205466318023639	3.533196051491474E-4
88	3.6804125536369523E-6	0.0030621032446259445	0.0	6.2054773592613	3.570000177027844E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21880	0.0	39.138977	1
GTATCAA	34990	0.0	32.112648	1
TCAACGC	38205	0.0	27.845707	4
ATCAACG	38260	0.0	27.817001	3
CAACGCA	39260	0.0	27.119501	5
AACGCAG	41115	0.0	26.085575	6
TATCAAC	42655	0.0	25.428135	2
ACGCAGA	49900	0.0	21.345585	7
CGCAGAG	50600	0.0	21.02461	8
GTACATG	84830	0.0	20.419827	1
TACATGG	83690	0.0	20.149345	2
ACATGGG	85655	0.0	19.09037	3
CATGGGG	64515	0.0	17.873	4
GCAGAGT	65715	0.0	16.070127	9
ACCGAAC	3355	0.0	16.009556	8
AGAGTAC	54825	0.0	15.687053	10-11
TATACCG	2625	0.0	15.016275	5
CAGAGTA	63475	0.0	14.740125	10-11
GTACTAG	6800	0.0	14.651059	1
GTGGTAT	13670	0.0	14.480975	1
>>END_MODULE