FastQCFastQC Report
Fri 27 May 2016
SRR2139269_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139269_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8754447
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66150.035.93291
GTATCAA107600.030.4986761
TCAACGC112950.027.580594
ATCAACG113750.027.4241333
CAACGCA115850.026.8533485
AACGCAG121300.025.6820096
TATCAAC129800.024.6248782
ACGCAGA146100.021.3225737
CGCAGAG150600.020.6854428
GTACATG258700.019.7783321
TACATGG251700.019.734982
ACATGGG254350.018.7743663
CATGGGG180500.017.5425874
GCAGAGT198350.015.4905649
AGAGTAC162550.015.29240910-11
CAGAGTA186300.014.3164110-11
GTATAAT54650.013.508991
TATACTG52650.013.2926755
ATGGGGG107300.013.2437645
TAGCCCT54300.013.20311454
Produced by FastQC (version 0.11.5)