##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139269_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8754447
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880156793455942	33.0	31.0	34.0	30.0	34.0
2	31.88163786930231	33.0	31.0	34.0	30.0	34.0
3	32.01077760822585	34.0	31.0	34.0	30.0	34.0
4	35.50304925028389	37.0	35.0	37.0	33.0	37.0
5	35.520346630689524	37.0	35.0	37.0	33.0	37.0
6	35.5734695749486	37.0	35.0	37.0	33.0	37.0
7	35.47490138440498	37.0	35.0	37.0	33.0	37.0
8	35.459837954356225	37.0	35.0	37.0	33.0	37.0
9	37.067660698614084	39.0	37.0	39.0	33.0	39.0
10-11	37.06109466423179	39.0	37.0	39.0	33.0	39.0
12-13	37.02826843317459	39.0	37.0	39.0	33.0	39.0
14-15	38.39600513887399	40.0	38.0	41.0	33.0	41.0
16-17	38.405902965658484	40.0	38.0	41.0	33.0	41.0
18-19	38.41372950227468	40.0	38.0	41.0	33.0	41.0
20-21	38.47269681267379	40.0	38.0	41.0	33.0	41.0
22-23	38.45488310750359	40.0	38.0	41.0	33.0	41.0
24-25	38.46355415219988	40.0	38.0	41.0	33.0	41.0
26-27	38.44472915773849	40.0	38.0	41.0	33.0	41.0
28-29	38.417439047291836	40.0	38.0	41.0	33.0	41.0
30-31	38.36878187842251	40.0	38.0	41.0	33.0	41.0
32-33	38.33012229379606	40.0	38.0	41.0	33.0	41.0
34-35	38.27386107505363	40.0	38.0	41.0	33.0	41.0
36-37	38.215689331599535	40.0	38.0	41.0	33.0	41.0
38-39	38.137270708651094	40.0	38.0	41.0	33.0	41.0
40-41	38.073107135108785	40.0	38.0	41.0	33.0	41.0
42-43	37.98811004966851	40.0	37.5	41.0	32.0	41.0
44-45	37.868204164898046	40.0	37.0	41.0	32.0	41.0
46-47	37.76757974910553	40.0	37.0	41.0	32.0	41.0
48-49	37.66847798771765	40.0	37.0	41.0	32.0	41.0
50-51	37.53522078353667	40.0	36.5	41.0	31.0	41.0
52-53	37.4110897767315	40.0	36.0	41.0	31.0	41.0
54-55	37.27225864343072	39.0	36.0	41.0	31.0	41.0
56-57	37.09563231206958	39.0	35.5	41.0	31.0	41.0
58-59	37.245341491887515	39.0	35.5	41.0	31.0	41.0
60-61	37.30926645351252	39.0	35.5	41.0	31.0	41.0
62-63	37.15384628651465	39.0	35.0	41.0	31.0	41.0
64-65	36.95212482300286	39.0	35.0	40.5	31.0	41.0
66-67	36.68522527900976	38.0	35.0	40.0	31.0	41.0
68-69	36.412301301961286	37.5	35.0	40.0	31.0	41.0
70-71	36.173918886752	37.0	35.0	40.0	31.0	41.0
72-73	35.94018395050031	36.5	34.5	39.0	31.0	41.0
74-75	35.671609829982884	36.0	34.0	39.0	30.5	41.0
76-77	35.42230269810808	36.0	34.0	39.0	30.5	40.5
78-79	35.11670369643237	35.0	34.0	37.5	30.0	39.5
80-81	34.82513292033246	35.0	34.0	37.0	30.0	39.0
82-83	34.55990058250164	35.0	34.0	37.0	30.0	39.0
84-85	34.332306653975024	35.0	34.0	36.0	30.0	37.5
86-87	34.13756989307271	35.0	34.0	36.0	30.0	37.0
88-89	33.99360869113988	35.0	34.0	36.0	30.0	37.0
90-91	33.879861715886804	35.0	34.0	35.0	30.0	36.5
92-93	33.791650054566524	35.0	34.0	35.0	30.0	36.0
94-95	33.76484565577286	35.0	34.0	35.0	30.0	36.0
96-97	33.76526225637353	35.0	34.0	35.0	30.0	36.0
98-99	33.793847673280176	35.0	34.0	35.0	30.0	36.0
100	33.46189643369604	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	35.0
17	286.0
18	1121.0
19	3786.0
20	9374.0
21	18604.0
22	30544.0
23	43147.0
24	56880.0
25	71222.0
26	83611.0
27	90972.0
28	104036.0
29	128072.0
30	151739.0
31	175103.0
32	219409.0
33	284818.0
34	384496.0
35	538183.0
36	824700.0
37	1507798.0
38	2343115.0
39	1592473.0
40	90923.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.924515277778255	20.667290578148453	14.488088168218964	23.920105975854327
2	13.890037828774334	21.0306830345766	42.482991786916976	22.596287349732087
3	17.724123522593718	26.35948335742966	30.53312219492562	25.383270925051004
4	12.140652630600195	18.295387475645235	39.871610394123124	29.692349499631444
5	13.206769557847704	38.17202427983008	35.804330768878415	12.816875393443796
6	29.31199423561534	38.2759070904193	19.570842110301196	12.841256563664158
7	26.245966906415326	32.7092428235893	23.730673534339008	17.314116735656373
8	23.85178913662841	37.23437211218163	21.73098103523627	17.182857715953702
9	25.09721059479828	17.79210040337214	21.774339372892427	35.33634962893716
10-11	23.542024853775704	27.27179891311208	29.573978109062203	19.612198124050018
12-13	24.26643786083837	24.82305833646222	29.34483294703407	21.565670855665335
14-15	21.615150931898025	26.5004520560698	26.675460294741697	25.208936717290477
16-17	21.518502524518844	29.524742329335606	27.35825322018009	21.598501925965458
18-19	21.559606854476563	29.154839473535866	28.414957267324827	20.87059640466275
20-21	22.483256394489615	28.298271860257184	28.08581219607957	21.13265954917363
22-23	22.220429345141163	28.18521513000201	27.894214407260353	21.700141117596473
24-25	21.909588120557718	28.334186675773054	27.984154151867642	21.772071051801586
26-27	21.890693794347218	28.46636892923217	27.851814445583813	21.791122830836795
28-29	21.79715246386117	28.52190688035131	27.925907877877563	21.755032777909953
30-31	22.06705124678495	28.369125259080008	27.840151043953416	21.723672450181624
32-33	21.911243465668015	28.409783697009583	27.853318975600576	21.82565386172183
34-35	22.012450094133733	28.394244526351102	27.884100795150466	21.70920458436469
36-37	21.94120214097337	28.191523339584556	27.999338901720133	21.867935617721944
38-39	21.76821759509091	28.27691858065552	28.15240439180302	21.802459432450544
40-41	21.96333927349929	28.091803300108186	28.15016279119009	21.794694635202433
42-43	21.77408189178552	28.141767949847257	28.117339336919244	21.966810821447982
44-45	21.8301822634819	28.204604530044964	28.078478111056498	21.886735095416636
46-47	21.713253671301896	28.175164640186097	28.008076603383703	22.103505085128305
48-49	21.644808098956737	28.18965813126482	28.16597847222326	21.999555297555183
50-51	21.550043605165605	28.312014083930677	28.133381029706854	22.004561281196864
52-53	21.695156042926616	28.22911469434392	28.062486727494836	22.013242535234625
54-55	21.760154927359224	28.10266726903744	28.029419291593943	22.107758512009394
56-57	21.790983047847114	28.140845092899774	27.992329691424207	22.07584216782891
58-59	21.79890723400588	28.14369574415106	27.99506773170511	22.062329290137953
60-61	21.843746886459588	28.08750614119006	28.017581684464798	22.05116528788556
62-63	21.79967493583074	28.195179170683648	27.89238761327662	22.112758280208993
64-65	21.917252685894965	28.20541388919626	27.74075119842999	22.136582226478787
66-67	21.960392001085996	28.20566661876267	27.653589132959528	22.180352247191802
68-69	21.91957968711774	28.15530982433484	27.747837182994388	22.177273305553037
70-71	21.994945427015825	27.98953235135858	27.819564844105425	22.195957377520166
72-73	22.045204948811996	27.9973087054526	27.78090386838472	22.176582477350678
74-75	21.954535705822238	28.184201230112283	27.814334305847126	22.046928758218353
76-77	22.085518865284595	28.09608801581592	27.79007401820283	22.028319100696656
78-79	22.077727564506308	28.103724939218523	27.750884998618197	22.067662497656972
80-81	22.062466345810947	28.19369825353441	27.77719616480233	21.966639235852313
82-83	22.088850306995134	28.097482489168918	27.92890036953809	21.88476683429786
84-85	22.096369510319338	28.224174812950082	27.879715024444025	21.799740652286552
86-87	21.89675148625698	28.238502873038296	27.974411239665837	21.89033440103889
88-89	22.01384809753699	28.204004617010526	27.88688588273333	21.895261402719154
90-91	21.94587050901284	28.306452939174704	27.94978817332111	21.797888378491347
92-93	21.963979994694096	28.24892839509754	27.96427248406265	21.82281912614571
94-95	21.844160889917106	28.351455608874147	27.9808231128417	21.823560388367042
96-97	21.885712726766435	28.485559887160843	28.037569568047804	21.591157818024914
98-99	22.87843338362712	30.492744513330088	28.22506231621614	18.403759786826654
100	21.8422104629239	31.482482512989495	22.09651999267851	24.57878703140809
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2805.0
1	1998.0
2	1372.0
3	1519.5
4	1479.5
5	1581.0
6	1818.5
7	2108.0
8	2453.0
9	2822.5
10	3156.5
11	3417.0
12	3697.0
13	4183.0
14	4932.0
15	5977.5
16	7332.0
17	8992.5
18	10906.5
19	13449.0
20	17130.0
21	22305.0
22	29443.0
23	38560.0
24	50487.5
25	66132.5
26	85242.5
27	105966.0
28	126826.5
29	150379.5
30	171829.0
31	191504.5
32	212754.5
33	232373.5
34	252278.5
35	269847.0
36	288642.5
37	306974.0
38	318866.0
39	328808.0
40	341100.5
41	351408.0
42	356215.0
43	360907.5
44	366265.5
45	371492.0
46	373367.0
47	371342.5
48	365356.5
49	353685.5
50	342322.5
51	330300.5
52	312579.0
53	293049.5
54	272723.0
55	249609.0
56	226738.5
57	203900.5
58	181069.0
59	158544.0
60	134114.5
61	110723.5
62	89581.5
63	70387.0
64	54036.5
65	41105.5
66	31642.5
67	25169.5
68	19995.5
69	15328.5
70	11921.0
71	9419.5
72	7448.0
73	5743.5
74	4407.0
75	3431.0
76	2604.5
77	1908.0
78	1461.5
79	1153.0
80	879.5
81	656.0
82	479.0
83	332.5
84	248.5
85	196.5
86	159.5
87	131.0
88	87.5
89	54.0
90	43.0
91	39.0
92	33.0
93	22.0
94	15.0
95	12.0
96	8.0
97	6.5
98	6.5
99	7.5
100	26.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	2.284553210499761E-4
6	0.0
7	1.1422766052498804E-5
8	1.1422766052498804E-5
9	0.0
10-11	2.8556915131247012E-5
12-13	9.138212841999044E-5
14-15	2.284553210499761E-5
16-17	7.424797934124223E-5
18-19	9.195326672261537E-4
20-21	3.9999238871626046E-4
22-23	0.0
24-25	8.5830077846164E-5
26-27	1.8325559287479348E-4
28-29	0.0
30-31	0.0
32-33	5.751986203976129E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	6.610025557964276E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.6792171916793697E-4
76-77	1.35512335552393E-5
78-79	6.17484950862606E-4
80-81	4.1709279647373065E-5
82-83	2.1131753312138185E-5
84-85	4.496843858017937E-4
86-87	1.085187979879168E-4
88-89	2.2005319859427082E-5
90-91	4.464911931472532E-5
92-93	6.269748290757083E-4
94-95	4.144644087515541E-4
96-97	4.2333318438276846E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2900.0
20-21	5538.0
22-23	6218.0
24-25	6778.0
26-27	8807.0
28-29	11608.0
30-31	15971.0
32-33	17572.0
34-35	22161.0
36-37	34436.0
38-39	40048.0
40-41	36279.0
42-43	43203.0
44-45	52663.0
46-47	59275.0
48-49	55549.0
50-51	55038.0
52-53	55578.0
54-55	61735.0
56-57	68990.0
58-59	71717.0
60-61	71382.0
62-63	70433.0
64-65	74590.0
66-67	78894.0
68-69	80736.0
70-71	80647.0
72-73	80530.0
74-75	83667.0
76-77	90378.0
78-79	94578.0
80-81	95020.0
82-83	93564.0
84-85	93005.0
86-87	94581.0
88-89	96440.0
90-91	99982.0
92-93	102291.0
94-95	117416.0
96-97	289155.0
98-99	524215.0
100-101	5610879.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.96779910718001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.23711178610604	75.82280987146824
2	7.541428897119809	12.815572310231163
3	1.8731277690921841	4.7746663195891506
4	0.6394822333187953	2.1734159173296885
5	0.298929142142963	1.269967564844247
6	0.15450543054661267	0.7876791830191765
7	0.08125826211297899	0.48330349837099223
8	0.0471459488451689	0.32047100081549573
9	0.03195214757532021	0.2443413290600499
>10	0.09354904581000241	1.1942211284630302
>50	0.0011890645871328947	0.06592454661419647
>100	3.202727430870793E-4	0.04762733019462722
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1422766052498804E-5	0.0
2	0.0	2.284553210499761E-5	0.0	1.1422766052498804E-5	1.1422766052498804E-5
3	0.0	2.284553210499761E-5	0.0	2.284553210499761E-5	1.1422766052498804E-5
4	0.0	7.995936236749163E-5	0.0	2.284553210499761E-5	1.1422766052498804E-5
5	1.1422766052498804E-5	9.138212841999044E-5	0.0	3.426829815749641E-5	1.1422766052498804E-5
6	1.1422766052498804E-5	9.138212841999044E-5	0.0	3.426829815749641E-5	1.1422766052498804E-5
7	1.1422766052498804E-5	9.138212841999044E-5	0.0	3.426829815749641E-5	1.1422766052498804E-5
8	1.1422766052498804E-5	1.0280489447248924E-4	0.0	5.7113830262494024E-5	1.1422766052498804E-5
9	1.1422766052498804E-5	1.9418702289247968E-4	0.0	1.2565042657748684E-4	1.1422766052498804E-5
10-11	1.1422766052498804E-5	1.9418702289247968E-4	0.0	1.7134149078748206E-4	2.284553210499761E-5
12-13	1.1422766052498804E-5	1.9418702289247968E-4	0.0	2.0560978894497848E-4	2.284553210499761E-5
14-15	1.1422766052498804E-5	3.198374494699665E-4	0.0	2.684350022337219E-4	4.569106420999522E-5
16-17	1.1422766052498804E-5	3.312602155224653E-4	0.0	3.5981713065371236E-4	5.7113830262494024E-5
18-19	1.1422766052498804E-5	3.4268298157496413E-4	0.0	3.7695127973246055E-4	5.7113830262494024E-5
20-21	1.1422766052498804E-5	3.5981713065371236E-4	0.0	4.055081948637076E-4	5.7113830262494024E-5
22-23	1.1422766052498804E-5	4.569106420999522E-4	0.0	6.625204310449306E-4	6.853659631499282E-5
24-25	2.284553210499761E-5	5.425813874936933E-4	0.0	0.0014278457565623506	9.138212841999044E-5
26-27	2.284553210499761E-5	5.597155365724414E-4	0.0	0.0031641061965421687	9.138212841999044E-5
28-29	2.284553210499761E-5	5.711383026249402E-4	0.0	0.014004311180363535	1.0851627749873864E-4
30-31	2.284553210499761E-5	6.739431970974295E-4	0.0	0.04902651189732487	1.1993904355123744E-4
32-33	2.855691513124701E-5	6.796545801236789E-4	0.0	0.1391121563703567	1.3707319262998565E-4
34-35	3.426829815749641E-5	7.881708576224175E-4	0.0	0.26379164783338116	1.4849595868248445E-4
36-37	3.426829815749641E-5	8.795529860424079E-4	0.0	0.43825726513622165	1.5991872473498326E-4
38-39	3.426829815749641E-5	8.852643690686573E-4	0.0	0.6271098562821844	1.6563010776123266E-4
40-41	3.426829815749641E-5	9.423781993311514E-4	0.0	0.8056191327676094	1.7134149078748206E-4
42-43	3.426829815749641E-5	9.538009653836502E-4	0.0	1.039740145779625	1.7134149078748206E-4
44-45	3.426829815749641E-5	0.0011022969240661346	0.0	1.2101792380489595	1.7134149078748206E-4
46-47	3.426829815749641E-5	0.0012507928827486191	0.0	1.3686301373461967	1.7134149078748206E-4
48-49	3.426829815749641E-5	0.0012565042657748686	0.0	1.48160129360541	1.7134149078748206E-4
50-51	3.426829815749641E-5	0.001393577458404854	0.0	1.6136084894911122	1.7134149078748206E-4
52-53	3.426829815749641E-5	0.0014050002244573529	0.0	1.7439822298313075	1.8276425683998087E-4
54-55	3.426829815749641E-5	0.0014050002244573529	0.0	1.8611455412317877	1.9418702289247968E-4
56-57	3.426829815749641E-5	0.0014392685226148492	0.0	1.9572909630956703	1.9989840591872908E-4
58-59	3.426829815749641E-5	0.001462114054719847	0.0	2.002428023152119	2.0560978894497848E-4
60-61	3.426829815749641E-5	0.0014963823528773433	0.0	2.0196878226574446	2.0560978894497848E-4
62-63	3.426829815749641E-5	0.0015078051189298422	0.0	2.030368108916531	2.284553210499761E-4
64-65	3.426829815749641E-5	0.0016791466097173242	0.0	2.0347030486334545	2.284553210499761E-4
66-67	4.569106420999522E-5	0.001736260439979818	0.0	2.0386439029215664	2.284553210499761E-4
68-69	4.569106420999522E-5	0.0017819515041898135	0.0	2.0424476840170485	2.284553210499761E-4
70-71	4.569106420999522E-5	0.00181621980234731	0.0	2.045983030110297	2.284553210499761E-4
72-73	4.569106420999522E-5	0.001833353951426058	0.0	2.0487816077931593	2.513008531549737E-4
74-75	4.569106420999522E-5	0.001901890547741051	0.0	2.0514374009003653	2.513008531549737E-4
76-77	5.7113830262494024E-5	0.002033252357344787	0.0	2.0540817712415187	2.5701223618122306E-4
78-79	5.7113830262494024E-5	0.0020789434215547826	0.0	2.0556809584888684	2.627236192074725E-4
80-81	5.7113830262494024E-5	0.0020789434215547826	0.0	2.0566347594542522	2.7985776828622067E-4
82-83	5.7113830262494024E-5	0.0020789434215547826	0.0	2.0571659180756936	3.198374494699665E-4
84-85	5.7113830262494024E-5	0.00210178895365978	0.0	2.057457198610032	3.198374494699665E-4
86-87	5.7113830262494024E-5	0.002176036933001022	0.0	2.057474332759111	3.198374494699665E-4
88	5.7113830262494024E-5	0.0023302442747097562	0.0	2.057480044142137	3.312602155224653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6615	0.0	35.9329	1
GTATCAA	10760	0.0	30.498676	1
TCAACGC	11295	0.0	27.58059	4
ATCAACG	11375	0.0	27.424133	3
CAACGCA	11585	0.0	26.853348	5
AACGCAG	12130	0.0	25.682009	6
TATCAAC	12980	0.0	24.624878	2
ACGCAGA	14610	0.0	21.322573	7
CGCAGAG	15060	0.0	20.685442	8
GTACATG	25870	0.0	19.778332	1
TACATGG	25170	0.0	19.73498	2
ACATGGG	25435	0.0	18.774366	3
CATGGGG	18050	0.0	17.542587	4
GCAGAGT	19835	0.0	15.490564	9
AGAGTAC	16255	0.0	15.292409	10-11
CAGAGTA	18630	0.0	14.31641	10-11
GTATAAT	5465	0.0	13.50899	1
TATACTG	5265	0.0	13.292675	5
ATGGGGG	10730	0.0	13.243764	5
TAGCCCT	5430	0.0	13.2031145	4
>>END_MODULE