FastQCFastQC Report
Fri 27 May 2016
SRR2139268_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139268_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12275236
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104650.039.9702221
GTATCAA174700.032.5123981
ATCAACG182250.029.7884373
TCAACGC184750.029.3615134
CAACGCA191350.028.3257715
AACGCAG201550.026.9578046
TATCAAC208200.026.7100542
ACGCAGA239950.022.5519087
CGCAGAG242150.022.3106528
TCGGTGG51600.021.96115994
GTACATG427900.021.1765541
TACATGG427200.020.7474482
ACATGGG431500.020.0304933
CATGGGG281200.017.8031714
CTCGGTG69800.017.5640794
GCAGAGT309100.017.4924879
GTGGTAT66500.016.0230751
AGAGTAC270250.015.78738810-11
CAGAGTA299300.015.57171210-11
TCTCGGT70550.015.51168992-93
Produced by FastQC (version 0.11.5)