##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139268_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12275236
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76203732457771	33.0	31.0	34.0	30.0	34.0
2	31.766206205729976	33.0	31.0	34.0	29.0	34.0
3	31.897216721535944	34.0	31.0	34.0	30.0	34.0
4	35.38715255657814	37.0	35.0	37.0	33.0	37.0
5	35.404875311562236	37.0	35.0	37.0	33.0	37.0
6	35.457667534864505	37.0	35.0	37.0	33.0	37.0
7	35.3510456336644	37.0	35.0	37.0	32.0	37.0
8	35.33243092026907	37.0	35.0	37.0	32.0	37.0
9	36.90880672273836	39.0	37.0	39.0	33.0	39.0
10-11	36.90955599550184	39.0	37.0	39.0	33.0	39.0
12-13	36.87581301899206	39.0	37.0	39.0	33.0	39.0
14-15	38.22004807076622	40.0	38.0	41.0	33.0	41.0
16-17	38.23489780563078	40.0	38.0	41.0	33.0	41.0
18-19	38.250138816068386	40.0	38.0	41.0	33.0	41.0
20-21	38.320414444877144	40.0	38.0	41.0	33.0	41.0
22-23	38.30587051586215	40.0	38.0	41.0	33.0	41.0
24-25	38.319054530739194	40.0	38.0	41.0	33.0	41.0
26-27	38.30605552423517	40.0	38.0	41.0	33.0	41.0
28-29	38.2851677922112	40.0	38.0	41.0	33.0	41.0
30-31	38.239619926367666	40.0	38.0	41.0	33.0	41.0
32-33	38.20114321839905	40.0	38.0	41.0	33.0	41.0
34-35	38.148962735767284	40.0	38.0	41.0	33.0	41.0
36-37	38.09467568766167	40.0	38.0	41.0	32.5	41.0
38-39	38.018673660304216	40.0	38.0	41.0	32.0	41.0
40-41	37.95753908582583	40.0	37.5	41.0	32.0	41.0
42-43	37.872603062374154	40.0	37.0	41.0	32.0	41.0
44-45	37.756891605790855	40.0	37.0	41.0	32.0	41.0
46-47	37.66520717828921	40.0	37.0	41.0	32.0	41.0
48-49	37.57232085764444	40.0	37.0	41.0	31.0	41.0
50-51	37.44596798007433	40.0	36.0	41.0	31.0	41.0
52-53	37.33663916801935	39.5	36.0	41.0	31.0	41.0
54-55	37.208890566552554	39.0	36.0	41.0	31.0	41.0
56-57	37.04495037345126	39.0	35.5	41.0	30.5	41.0
58-59	37.21187006029733	39.0	35.5	41.0	31.0	41.0
60-61	37.28912223493946	39.0	35.5	41.0	31.0	41.0
62-63	37.14755349051525	39.0	35.0	41.0	31.0	41.0
64-65	36.95151850598684	39.0	35.0	40.5	31.0	41.0
66-67	36.686357296599596	38.0	35.0	40.0	31.0	41.0
68-69	36.41413959239726	37.5	35.0	40.0	31.0	41.0
70-71	36.17278863186504	37.0	35.0	40.0	30.0	41.0
72-73	35.92455016578859	37.0	34.5	39.0	30.0	41.0
74-75	35.639355056765254	36.0	34.0	39.0	30.0	41.0
76-77	35.36100965854784	36.0	34.0	39.0	30.0	40.5
78-79	35.01481285214345	35.0	34.0	37.5	30.0	39.5
80-81	34.681479639538296	35.0	34.0	37.0	30.0	39.0
82-83	34.38038327386839	35.0	34.0	37.0	30.0	39.0
84-85	34.12762735524772	35.0	34.0	36.0	29.5	37.5
86-87	33.904458314996255	35.0	34.0	36.0	29.0	37.0
88-89	33.73316143123563	35.0	34.0	35.5	29.0	37.0
90-91	33.5842518397517	35.0	33.0	35.0	29.0	36.0
92-93	33.46939106071372	35.0	33.0	35.0	29.0	36.0
94-95	33.438008358398065	35.0	33.0	35.0	29.0	36.0
96-97	33.45062731670143	35.0	33.0	35.0	29.0	36.0
98-99	33.55595607238786	35.0	33.0	35.0	30.0	36.0
100	33.18616555590867	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	64.0
17	507.0
18	1899.0
19	5779.0
20	14700.0
21	29755.0
22	48381.0
23	68939.0
24	89510.0
25	111317.0
26	127599.0
27	135711.0
28	154449.0
29	189976.0
30	225585.0
31	262199.0
32	329844.0
33	431417.0
34	580823.0
35	808689.0
36	1244803.0
37	2238866.0
38	3276426.0
39	1795858.0
40	102136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.731257631217844	20.96108783570434	14.567133373240237	23.74052115983758
2	13.768142624712063	21.258018990429186	42.62430473841806	22.349533646440687
3	17.547719652803416	26.72197096658671	30.73782858431398	24.992480796295894
4	11.897115460753668	18.407800876496385	40.24732396183666	29.447759700913284
5	12.838187586790747	38.612711762092296	35.94728368890351	12.60181696221345
6	28.918222020334273	38.64113081003086	19.737991188112392	12.702655981522474
7	25.982359931817196	33.101139562612076	23.800935476922806	17.11556502864792
8	23.73452728196242	37.36269814793769	21.826612688066664	17.076161882033215
9	25.117928486262915	17.797295302509866	21.864206928485938	35.22056928274129
10-11	23.291077244725212	27.559969737437978	29.696870112363648	19.45208290547316
12-13	24.04717828367124	25.150787472391546	29.52303355115964	21.279000692777583
14-15	21.445091918010846	26.731284856263017	26.948009866696626	24.87561335902952
16-17	21.239402943734227	29.810146046202817	27.60239018022073	21.348060829842222
18-19	21.247566878236803	29.43134878743195	28.694374652218425	20.626709682112825
20-21	22.367955768727548	28.515800413995752	28.288299589048517	20.827944228228187
22-23	22.007202386506215	28.44238808521633	28.06984311801318	21.480566410264274
24-25	21.66193355723169	28.587907529782104	28.192420691238024	21.557738221748178
26-27	21.590856921222702	28.745171556281974	28.090191726159542	21.57377979633578
28-29	21.463496890447534	28.814215760555832	28.18991327239866	21.532374076597975
30-31	21.804282470031005	28.56717656330089	28.152219995730093	21.476320970938012
32-33	21.618304842820006	28.648275753471854	28.0846793721346	21.648740031573535
34-35	21.736209266522525	28.610377311489344	28.15407504302683	21.4993383789613
36-37	21.641271808940704	28.369009085166663	28.27947457450739	21.710244531385243
38-39	21.454323809423236	28.351829710784614	28.508323452354983	21.685523027437164
40-41	21.653736217344875	28.17056852189618	28.530371669748746	21.645323591010204
42-43	21.45347003491192	28.153867721750082	28.50517910370262	21.887483139635375
44-45	21.430517491763858	28.179105315176496	28.5452759013599	21.845101291699738
46-47	21.323254322425385	28.052801726870687	28.588353808437567	22.03559014226636
48-49	21.14537303673125	28.013507616326493	28.79613946265293	22.044979884289322
50-51	21.02140044668614	28.057739570909828	28.797018855367707	22.123841127036325
52-53	21.027423993636756	27.996555469883823	28.82534695484626	22.150673581633164
54-55	20.988501936991685	27.903164645967475	28.90087022323118	22.207463193809662
56-57	20.99120629239211	27.94883769270627	28.765333668397204	22.29462234650442
58-59	20.920934671089423	27.97791775215587	28.750588133716704	22.350559443038
60-61	20.947138402265345	27.801242822363463	28.734953970913196	22.516664804457992
62-63	20.93493774311948	27.736554944212394	28.679438921355633	22.64906839131249
64-65	21.109838298991676	27.790648590913776	28.576532124769855	22.522980985324697
66-67	20.944837742243212	27.947581313197666	28.265124843087502	22.842456101471615
68-69	21.041253218073027	28.05405734759978	28.097934305558148	22.80675512876904
70-71	21.18730472235294	28.038966743474454	27.92629305625703	22.84743547791558
72-73	21.327567624928285	27.980434616800366	27.55320181416423	23.138795944107116
74-75	21.420617939203474	27.91603015025526	27.36119399143403	23.302157919107238
76-77	21.643871956744658	27.73734691359643	27.463882703693777	23.154898425965133
78-79	21.804025893995593	27.452981908416447	27.63412330141627	23.108868896171693
80-81	21.95063794670178	27.35140848988372	27.61472377278712	23.083229790627378
82-83	21.92391340421005	27.48498555599252	27.533078972388804	23.058022067408626
84-85	22.031556929393858	27.31036833767493	27.644334210296023	23.01374052263519
86-87	21.714848535025176	27.539314128115635	27.87675433765489	22.869082999204302
88-89	21.865817810012956	27.701464785051677	27.66008248243083	22.772634922504533
90-91	21.797779784042902	27.95156689411695	27.945732568839908	22.30492075300024
92-93	21.504087180677775	27.923755456836336	28.246845346765692	22.325312015720197
94-95	21.34602688572435	27.954298922185938	28.4941767516372	22.205497440452515
96-97	21.453652323137224	27.85465453324749	28.79935220242841	21.892340941186877
98-99	22.781711420619295	29.885860698051147	28.94179029382596	18.3906375875036
100	22.179132484078618	31.15015215085367	22.63197086505338	24.038744500014335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5258.0
1	3797.0
2	2515.0
3	2646.5
4	2560.5
5	2622.0
6	2946.0
7	3439.5
8	3903.0
9	4459.5
10	5003.0
11	5420.5
12	6005.0
13	6906.0
14	7941.0
15	9180.5
16	10762.0
17	12687.0
18	15205.0
19	18681.0
20	23507.5
21	29750.0
22	38073.0
23	49059.5
24	63392.0
25	82073.5
26	105825.5
27	132808.0
28	161280.0
29	192504.0
30	221811.5
31	248781.0
32	277456.5
33	304971.0
34	332486.0
35	358940.5
36	386340.5
37	414456.0
38	433842.5
39	448265.0
40	465130.5
41	478346.5
42	487853.5
43	499184.5
44	509513.0
45	516913.0
46	518752.0
47	516580.0
48	509953.5
49	496617.5
50	481301.5
51	459080.5
52	428757.0
53	396220.0
54	361499.5
55	325241.0
56	289789.0
57	253751.0
58	220863.5
59	191631.5
60	160290.5
61	129870.0
62	102254.5
63	79246.0
64	60474.5
65	45465.0
66	34513.5
67	26844.0
68	20914.0
69	15656.0
70	12098.0
71	9583.0
72	7439.0
73	5747.0
74	4499.0
75	3480.5
76	2676.0
77	2039.0
78	1525.0
79	1142.5
80	887.5
81	687.5
82	526.0
83	370.5
84	267.0
85	203.5
86	160.0
87	133.0
88	95.0
89	65.5
90	56.5
91	50.5
92	33.0
93	17.0
94	12.5
95	8.0
96	5.5
97	7.5
98	6.5
99	6.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.792226234998659E-4
6	0.0
7	0.0
8	8.146482886357541E-6
9	0.0
10-11	2.4439448659072623E-5
12-13	9.775779463629049E-5
14-15	2.4439448659072623E-5
16-17	8.961131174993295E-5
18-19	9.001863589425083E-4
20-21	4.319823601225835E-4
22-23	0.0
24-25	1.0202415515735574E-4
26-27	1.7971589204760445E-4
28-29	0.0
30-31	0.0
32-33	7.791271028999316E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	5.422869073169104E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.429953504168091E-4
76-77	9.038644362425776E-6
78-79	3.3856379317357484E-4
80-81	1.3908719761226226E-5
82-83	2.350376556178442E-5
84-85	4.5281041071191185E-4
86-87	1.015846037213633E-4
88-89	1.4747789208079113E-5
90-91	3.0022247986870667E-5
92-93	5.864036151833868E-4
94-95	4.216313761204854E-4
96-97	3.7394165164044465E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4258.0
20-21	7893.0
22-23	8772.0
24-25	9836.0
26-27	12544.0
28-29	16009.0
30-31	18398.0
32-33	18646.0
34-35	21557.0
36-37	28454.0
38-39	31194.0
40-41	27343.0
42-43	19218.0
44-45	19275.0
46-47	20305.0
48-49	20278.0
50-51	20028.0
52-53	19865.0
54-55	20181.0
56-57	19965.0
58-59	20234.0
60-61	22159.0
62-63	64456.0
64-65	110564.0
66-67	117805.0
68-69	119907.0
70-71	118666.0
72-73	118308.0
74-75	122975.0
76-77	132318.0
78-79	142228.0
80-81	148013.0
82-83	146253.0
84-85	151786.0
86-87	161699.0
88-89	175303.0
90-91	184667.0
92-93	196549.0
94-95	218609.0
96-97	456196.0
98-99	820228.0
100-101	8162294.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.25001569839927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.51988862872851	70.29742309307949
2	9.07844732724922	14.752479757121801
3	2.4882706452234853	6.065160869588228
4	0.9233263535953211	3.0008112289746243
5	0.40920394182390385	1.6623913348519526
6	0.21617536439386964	1.0538551050365452
7	0.1223139547513177	0.6956607520574503
8	0.07103669118990878	0.4617385819473944
9	0.04262262124371459	0.3116779780642863
>10	0.12661582738543112	1.5018329359486577
>50	0.0016815587815032988	0.08951732786454282
>100	3.3274785452672595E-4	0.04768511587518212
>500	6.747135144011187E-5	0.03977181956119165
>1k	1.686642793531185E-5	0.019994100028646915
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.6292965772715082E-5	0.0	0.0	0.0
3	0.0	1.6292965772715082E-5	0.0	0.0	0.0
4	0.0	5.7025380204502786E-5	0.0	0.0	0.0
5	0.0	5.7025380204502786E-5	0.0	1.6292965772715082E-5	0.0
6	0.0	5.7025380204502786E-5	0.0	1.6292965772715082E-5	0.0
7	0.0	5.7025380204502786E-5	0.0	1.6292965772715082E-5	0.0
8	0.0	9.775779463629049E-5	0.0	1.6292965772715082E-5	2.4439448659072623E-5
9	0.0	1.2219724329536313E-4	0.0	4.8878897318145245E-5	3.2585931545430164E-5
10-11	0.0	1.2219724329536313E-4	0.0	1.0183103607946925E-4	3.2585931545430164E-5
12-13	0.0	1.2219724329536313E-4	0.0	1.547831748407933E-4	3.2585931545430164E-5
14-15	0.0	2.0366207215893854E-4	0.0	1.9551558927258098E-4	5.7025380204502786E-5
16-17	0.0	2.1995503793165362E-4	0.0	2.3217476226118994E-4	5.7025380204502786E-5
18-19	0.0	2.4439448659072625E-4	0.0	2.973466253520503E-4	5.7025380204502786E-5
20-21	0.0	2.688339352497989E-4	0.0	4.195438686474134E-4	5.7025380204502786E-5
22-23	0.0	3.0141986679522905E-4	0.0	7.820623570903239E-4	6.109862164768156E-5
24-25	0.0	3.462255226701955E-4	0.0	0.001828885407987268	7.331834597721787E-5
26-27	0.0	3.7881145421562567E-4	0.0	0.004590543106462475	8.961131174993295E-5
28-29	0.0	3.9103117854516196E-4	0.0	0.019628950514678493	8.961131174993295E-5
30-31	0.0	4.4805655874966476E-4	0.0	0.07110250263212863	9.368455319311173E-5
32-33	0.0	4.4805655874966476E-4	0.0	0.19792287496549965	9.775779463629049E-5
34-35	0.0	5.254481461700614E-4	0.0	0.38098249190483996	9.775779463629049E-5
36-37	0.0	5.784002849313854E-4	0.0	0.6586187019133481	9.775779463629049E-5
38-39	0.0	5.987664921472792E-4	0.0	1.0253244825598467	9.775779463629049E-5
40-41	0.0	6.76158079567676E-4	0.0	1.406180703979948	1.0183103607946925E-4
42-43	0.0	6.883778038972123E-4	0.0	1.853878817482613	1.0590427752264803E-4
44-45	0.0	7.454031841017149E-4	0.0	2.4146460402064776	1.0590427752264803E-4
46-47	0.0	7.861355985335028E-4	0.0	3.045273426922301	1.2219724329536313E-4
48-49	0.0	7.942820814198603E-4	0.0	3.6803243538454167	1.4663669195443574E-4
50-51	0.0	9.124060832720446E-4	0.0	4.30560764778779	1.4663669195443574E-4
52-53	0.0	9.286990490447597E-4	0.0	4.914439119541164	1.547831748407933E-4
54-55	0.0	9.327722904879385E-4	0.0	5.543351671609409	1.6292965772715082E-4
56-57	0.0	9.368455319311173E-4	0.0	6.234580744516847	1.6292965772715082E-4
58-59	0.0	9.368455319311173E-4	0.0	6.946310441607803	1.6292965772715082E-4
60-61	4.0732414431787704E-6	9.612849805901899E-4	0.0	7.648891638417379	1.6292965772715082E-4
62-63	8.146482886357541E-6	9.694314634765474E-4	0.0	8.318870610715752	1.6292965772715082E-4
64-65	8.146482886357541E-6	0.0010753357409991956	0.0	8.492398842678055	1.6292965772715082E-4
66-67	8.146482886357541E-6	0.0010916287067719106	0.0	8.496211396668869	1.6292965772715082E-4
68-69	8.146482886357541E-6	0.0011323611212036983	0.0	8.499694018102788	1.6292965772715082E-4
70-71	8.146482886357541E-6	0.0011405076040900558	0.0	8.502618605458991	1.6292965772715082E-4
72-73	8.146482886357541E-6	0.0011405076040900558	0.0	8.505091063015001	1.8736910638622345E-4
74-75	8.146482886357541E-6	0.0011771667770786648	0.0	8.50716434290958	1.8736910638622345E-4
76-77	8.146482886357541E-6	0.0012382653987263463	0.0	8.509107279077973	1.8736910638622345E-4
78-79	8.146482886357541E-6	0.0012545583644990614	0.0	8.510703989723702	1.8736910638622345E-4
80-81	8.146482886357541E-6	0.0012545583644990614	0.0	8.51151049152945	1.9144234782940222E-4
82-83	8.146482886357541E-6	0.0012545583644990614	0.0	8.512011500226961	2.0366207215893854E-4
84-85	8.146482886357541E-6	0.0012667780888285978	0.0	8.512170356643246	2.0366207215893854E-4
86-87	8.146482886357541E-6	0.0012912175374876701	0.0	8.51221516229912	2.077353136021173E-4
88	8.146482886357541E-6	0.0013767556077944245	0.0	8.512227382023449	2.1180855504529606E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10465	0.0	39.970222	1
GTATCAA	17470	0.0	32.512398	1
ATCAACG	18225	0.0	29.788437	3
TCAACGC	18475	0.0	29.361513	4
CAACGCA	19135	0.0	28.325771	5
AACGCAG	20155	0.0	26.957804	6
TATCAAC	20820	0.0	26.710054	2
ACGCAGA	23995	0.0	22.551908	7
CGCAGAG	24215	0.0	22.310652	8
TCGGTGG	5160	0.0	21.961159	94
GTACATG	42790	0.0	21.176554	1
TACATGG	42720	0.0	20.747448	2
ACATGGG	43150	0.0	20.030493	3
CATGGGG	28120	0.0	17.803171	4
CTCGGTG	6980	0.0	17.56407	94
GCAGAGT	30910	0.0	17.492487	9
GTGGTAT	6650	0.0	16.023075	1
AGAGTAC	27025	0.0	15.787388	10-11
CAGAGTA	29930	0.0	15.571712	10-11
TCTCGGT	7055	0.0	15.511689	92-93
>>END_MODULE