FastQCFastQC Report
Fri 27 May 2016
SRR2139267_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139267_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10875776
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77450.034.9373551
GTATCAA129850.030.2076971
TCAACGC132400.028.3506164
ATCAACG133700.028.0425763
CAACGCA137200.027.3587595
AACGCAG143850.026.1540726
TATCAAC153700.024.9287682
ACGCAGA173850.021.4914387
TGTAGAT160550.021.19974794
CGCAGAG179550.020.8332848
GTACATG310100.020.0067161
GTGTAGA163550.019.7072794
TACATGG303750.019.6837942
GGTGTAG175650.019.59747594
ACATGGG305250.018.8637223
CATGGGG226000.017.0303964
AGGTGTA216100.016.94340394
GCAGAGT234300.015.8911589
AGAGTAC194450.015.34054110-11
TAGGTGT234400.014.76806894
Produced by FastQC (version 0.11.5)