##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139267_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10875776
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.804052051090423	33.0	31.0	34.0	30.0	34.0
2	31.915571449798158	33.0	31.0	34.0	30.0	34.0
3	31.967161791489637	34.0	31.0	34.0	30.0	34.0
4	35.47143891157744	37.0	35.0	37.0	33.0	37.0
5	35.512873012463665	37.0	35.0	37.0	33.0	37.0
6	35.59859305671614	37.0	35.0	37.0	33.0	37.0
7	35.527287616074474	37.0	35.0	37.0	33.0	37.0
8	35.498794384878835	37.0	35.0	37.0	33.0	37.0
9	37.08426157361093	39.0	37.0	39.0	33.0	39.0
10-11	37.06307393605753	39.0	37.0	39.0	33.0	39.0
12-13	37.00862480985265	39.0	37.0	39.0	33.0	39.0
14-15	38.37067221686066	40.0	38.0	41.0	33.0	41.0
16-17	38.379532688058305	40.0	38.0	41.0	33.0	41.0
18-19	38.39279670710393	40.0	38.0	41.0	33.0	41.0
20-21	38.44571130356732	40.0	38.0	41.0	33.0	41.0
22-23	38.42867576267396	40.0	38.0	41.0	33.0	41.0
24-25	38.43924464331894	40.0	38.0	41.0	33.0	41.0
26-27	38.416060153962704	40.0	38.0	41.0	33.0	41.0
28-29	38.39070016631643	40.0	38.0	41.0	33.0	41.0
30-31	38.342804189194695	40.0	38.0	41.0	33.0	41.0
32-33	38.30050889280173	40.0	38.0	41.0	33.0	41.0
34-35	38.24481734739844	40.0	38.0	41.0	33.0	41.0
36-37	38.1885362379724	40.0	38.0	41.0	33.0	41.0
38-39	38.10956898845768	40.0	38.0	41.0	33.0	41.0
40-41	38.04599650131688	40.0	38.0	41.0	32.5	41.0
42-43	37.963097176567935	40.0	37.5	41.0	32.0	41.0
44-45	37.843746763503205	40.0	37.0	41.0	32.0	41.0
46-47	37.74234693050796	40.0	37.0	41.0	32.0	41.0
48-49	37.64283008721372	40.0	37.0	41.0	31.5	41.0
50-51	37.51252775424952	40.0	36.0	41.0	31.0	41.0
52-53	37.39264087209831	40.0	36.0	41.0	31.0	41.0
54-55	37.25600832902842	39.0	36.0	41.0	31.0	41.0
56-57	37.08402128949844	39.0	35.5	41.0	31.0	41.0
58-59	37.236339627880106	39.0	35.5	41.0	31.0	41.0
60-61	37.304690411282245	39.0	35.5	41.0	31.0	41.0
62-63	37.15388700697388	39.0	35.0	41.0	31.0	41.0
64-65	36.957300883401345	39.0	35.0	40.5	31.0	41.0
66-67	36.699753620396024	38.0	35.0	40.0	31.0	41.0
68-69	36.437120382995026	37.5	35.0	40.0	31.0	41.0
70-71	36.207335442495605	37.0	35.0	40.0	31.0	41.0
72-73	35.97677693997133	37.0	35.0	39.0	31.0	41.0
74-75	35.691364721388524	36.0	34.0	39.0	30.5	41.0
76-77	35.42956291482215	36.0	34.0	39.0	30.0	40.5
78-79	35.10247561192499	35.0	34.0	37.5	30.0	39.5
80-81	34.792720979291445	35.0	34.0	37.0	30.0	39.0
82-83	34.50008214782206	35.0	34.0	36.5	30.0	39.0
84-85	34.25330257197231	35.0	34.0	36.0	30.0	37.5
86-87	34.059072774437965	35.0	34.0	36.0	30.0	37.0
88-89	33.93389710062303	35.0	34.0	35.5	30.0	37.0
90-91	33.81733017082099	35.0	34.0	35.0	30.0	36.0
92-93	33.71694815113702	35.0	34.0	35.0	30.0	36.0
94-95	33.6731004743837	35.0	34.0	35.0	30.0	36.0
96-97	33.665522824338524	35.0	34.0	35.0	30.0	36.0
98-99	33.69257930232503	35.0	34.0	35.0	30.0	36.0
100	33.33754933264174	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	35.0
17	400.0
18	1435.0
19	4527.0
20	11234.0
21	22998.0
22	38190.0
23	54445.0
24	71812.0
25	89980.0
26	105437.0
27	115097.0
28	132380.0
29	161814.0
30	192331.0
31	224520.0
32	280735.0
33	363312.0
34	490565.0
35	685009.0
36	1050827.0
37	1918697.0
38	2971039.0
39	1795056.0
40	93901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.85219298374663	20.64266494639095	14.495002471547776	24.010139598314638
2	13.917903421328282	21.082973757458777	42.2245364376758	22.774586383537137
3	17.700070321418902	26.19794670283757	30.589109227700167	25.512873748043358
4	12.153606326573845	18.19654983699554	39.8000473713324	29.849796465098215
5	13.159634482488485	38.19969146058601	35.86075563652667	12.779918420398836
6	29.507439285251923	38.19502167017783	19.453241773276684	12.844297271293561
7	26.265224660750647	32.8056315245919	23.599603375428106	17.329540439229348
8	23.961177574823168	37.0827332228983	21.719424894370757	17.236664307907777
9	25.10903130038721	17.68782291948639	21.654620323184297	35.54852545694211
10-11	23.538528087220648	27.235483526757893	29.55903780085159	19.66695058516987
12-13	24.237130710477345	24.76830711364639	29.34855941864506	21.6460027572312
14-15	21.61880156759418	26.420850598771178	26.652668582484335	25.30767925115031
16-17	21.55757021815428	29.43379668918048	27.3377972945631	21.67083579810214
18-19	21.57730899461413	29.110409238966778	28.356639932693835	20.955641833725263
20-21	22.508388200206642	28.2717775487068	28.009277604477116	21.210556646609437
22-23	22.21506008037227	28.139753603091762	27.830546986555284	21.814639329980682
24-25	21.928621606837847	28.288312869821258	27.90053541118293	21.882530112157966
26-27	21.894758057378212	28.42411836468561	27.77462408982015	21.90649948811603
28-29	21.793726484933742	28.5233507607897	27.855592108906833	21.827330645369724
30-31	22.13804773349116	28.25828113807645	27.800754001096962	21.802917127335437
32-33	21.969908404915213	28.38880355594742	27.729571437483063	21.911716601654305
34-35	22.068832117334622	28.391603944518867	27.789628449632904	21.749935488513607
36-37	21.99953104765112	28.23534649922031	27.871395045496033	21.893727407632536
38-39	21.835021015207133	28.326690559035345	28.0283980889046	21.809890336852927
40-41	22.11805591119481	28.137430870219266	27.954214442752217	21.79029877583371
42-43	21.910071969928673	28.20307135790833	27.897213041495284	21.989643630667718
44-45	21.95782627413724	28.237366925014257	27.922807817736405	21.881998983112098
46-47	21.941402878649097	28.10318805459192	27.83598864311146	22.119420423647522
48-49	21.832946478392955	28.133118141236285	28.026414959079922	22.007520421290845
50-51	21.714892624295352	28.126462475366438	28.093650968569506	22.064993931768704
52-53	21.904649709895168	27.954116519593093	28.027551638582093	22.113682131929647
54-55	21.86355123238348	27.7156075348591	28.202783155401274	22.218058077356144
56-57	21.76297050813689	27.632348032049524	28.277510996545168	22.32717046326842
58-59	21.599652635781467	27.636479068966597	28.450415341815035	22.313452953436904
60-61	21.512169214550063	27.48603696629115	28.754157767077803	22.24763605208098
62-63	21.39115383559427	27.68051334837783	28.76354242281613	22.16479039321177
64-65	21.235777913764686	27.98710861340275	28.458492697653814	22.31862077517875
66-67	21.13859846694695	28.201926694453803	28.146218555307946	22.513256283291298
68-69	21.229343129659867	28.3785623245615	28.17452908122633	22.2175654645523
70-71	21.29268072485508	28.332530466233212	27.87925664014278	22.49553216876893
72-73	21.5794830217494	28.22800570903335	27.511288870757095	22.681222398460157
74-75	21.772590505722984	28.09241707522728	27.531600177559195	22.603392241490535
76-77	21.988323519193216	27.69713897667897	27.605006232695423	22.709531271432397
78-79	22.319386084592637	27.523637207203922	27.42754219355929	22.729434514644154
80-81	22.66371916524101	27.482012090568546	27.153779549021923	22.700489195168522
82-83	22.369250635475968	27.72346648416093	27.085076565427425	22.82220631493568
84-85	22.815656136444208	27.704065460231657	27.023674348194433	22.456604055129702
86-87	22.605176017047803	28.000012728240236	27.072447803608167	22.322363451103797
88-89	22.54229369174496	28.23162947991217	26.944661513414463	22.28141531492841
90-91	22.71008367991422	28.312124258997596	27.34580180814951	21.63199025293867
92-93	22.84662444585401	28.072450278062373	27.55757308316053	21.523352192923088
94-95	22.522123361618974	28.13661912773441	27.949250928115227	21.39200658253139
96-97	22.779279935814277	27.87461484561586	28.22915464493734	21.116950573632522
98-99	23.47325091823707	29.970158477209115	28.682171250390393	17.87441935416342
100	22.66694363145754	31.179831668577762	22.59215218348032	23.56107251648438
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3630.0
1	2659.0
2	1947.0
3	2183.0
4	2157.0
5	2223.0
6	2482.5
7	2842.0
8	3150.0
9	3563.0
10	4071.0
11	4532.5
12	5096.5
13	5821.0
14	6675.0
15	7748.0
16	9088.5
17	10829.5
18	13174.0
19	16328.0
20	20518.0
21	26387.5
22	34422.5
23	44819.0
24	58292.5
25	75533.5
26	96968.5
27	121141.5
28	146526.0
29	173079.5
30	197400.0
31	221238.0
32	248104.5
33	274703.5
34	299962.0
35	322975.5
36	347897.5
37	371363.0
38	387501.5
39	399604.0
40	412726.0
41	425772.5
42	434417.5
43	443346.5
44	452064.5
45	460275.0
46	464313.5
47	462462.5
48	456716.0
49	445303.5
50	431906.5
51	414215.5
52	391200.0
53	366429.0
54	341942.0
55	315737.5
56	287493.0
57	257949.5
58	228919.0
59	201167.0
60	169612.0
61	138479.5
62	110776.5
63	86945.0
64	66796.0
65	50433.0
66	38822.0
67	30620.0
68	24422.0
69	18864.0
70	14722.5
71	11689.0
72	9079.5
73	6877.0
74	5176.5
75	3896.5
76	2920.5
77	2213.5
78	1743.5
79	1369.0
80	1035.5
81	785.5
82	597.0
83	406.0
84	286.5
85	222.5
86	176.0
87	143.0
88	93.5
89	51.5
90	34.0
91	25.5
92	22.0
93	15.5
94	10.5
95	8.0
96	6.5
97	5.0
98	2.5
99	10.5
100	32.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.6550543152047266E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.2986865488954535E-5
12-13	9.654483505360905E-5
14-15	1.838949239116363E-5
16-17	8.735008885802723E-5
18-19	9.286693657537632E-4
20-21	4.829570882967907E-4
22-23	0.0
24-25	9.672433816947347E-5
26-27	1.6595634278688274E-4
28-29	0.0
30-31	0.0
32-33	5.561494510758572E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	2.4326437437024934E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.9327128628570457E-4
76-77	0.0
78-79	4.3216159386317735E-4
80-81	3.776817494952418E-5
82-83	1.0913673064334575E-5
84-85	3.8628963179865614E-4
86-87	1.0606855612726506E-4
88-89	2.2606440020904174E-5
90-91	2.29062302541137E-5
92-93	6.039306243986463E-4
94-95	3.065801293154985E-4
96-97	3.3713405828795015E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3607.0
20-21	6834.0
22-23	7709.0
24-25	8754.0
26-27	11696.0
28-29	16447.0
30-31	25468.0
32-33	30263.0
34-35	39033.0
36-37	56102.0
38-39	61402.0
40-41	58173.0
42-43	61459.0
44-45	70777.0
46-47	76727.0
48-49	46003.0
50-51	45325.0
52-53	16960.0
54-55	16688.0
56-57	16717.0
58-59	16288.0
60-61	18059.0
62-63	86925.0
64-65	93097.0
66-67	98127.0
68-69	98647.0
70-71	97424.0
72-73	95329.0
74-75	96771.0
76-77	101317.0
78-79	104293.0
80-81	104887.0
82-83	102363.0
84-85	103217.0
86-87	107757.0
88-89	114138.0
90-91	120201.0
92-93	125348.0
94-95	150696.0
96-97	367980.0
98-99	693794.0
100-101	7302974.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.80359777134856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.12284673815883	72.968887557727
2	8.069912568773367	13.364355887893204
3	2.1109778082525668	5.243896720163654
4	0.7767366134754456	2.57266344466001
5	0.37032165865293465	1.533198283455812
6	0.18517437056054234	0.9199862458474678
7	0.1111686001181907	0.644361203429339
8	0.06892828143067353	0.45660077525246445
9	0.04361268896740666	0.32501587994856646
>10	0.1376452450306069	1.7036768363273596
>50	0.002060257142318363	0.11604735180297389
>100	5.596673642793597E-4	0.08281807921091468
>500	1.8501240381938443E-5	0.009865882078559481
>1k	3.7000832466653775E-5	0.0586258522027122
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.194746195581814E-6	0.0
2	0.0	4.597373097790907E-5	0.0	1.838949239116363E-5	0.0
3	0.0	4.597373097790907E-5	0.0	3.677898478232726E-5	0.0
4	0.0	8.275271576023633E-5	0.0	3.677898478232726E-5	0.0
5	0.0	8.275271576023633E-5	0.0	3.677898478232726E-5	0.0
6	0.0	8.275271576023633E-5	0.0	4.597373097790907E-5	0.0
7	0.0	8.275271576023633E-5	0.0	5.516847717349088E-5	0.0
8	0.0	9.194746195581814E-5	0.0	6.43632233690727E-5	0.0
9	0.0	1.930896701072181E-4	0.0	1.5631068532489083E-4	0.0
10-11	0.0	2.022844163027999E-4	0.0	2.620502665740817E-4	0.0
12-13	0.0	2.068817894005908E-4	0.0	3.0342662445419986E-4	0.0
14-15	0.0	2.850371320630362E-4	0.0	3.402056092365271E-4	0.0
16-17	0.0	3.0802399755199076E-4	0.0	3.6778984782327256E-4	0.0
18-19	0.0	3.218161168453635E-4	0.0	3.861793402144362E-4	0.0
20-21	0.0	3.402056092365271E-4	0.0	4.643346828768816E-4	0.0
22-23	0.0	3.999714595078089E-4	0.0	5.93061129615027E-4	0.0
24-25	4.597373097790907E-6	4.505425635835089E-4	0.0	7.677613073310814E-4	0.0
26-27	9.194746195581814E-6	4.781268021702543E-4	0.0	0.0012504854825991269	0.0
28-29	9.194746195581814E-6	4.96516294561418E-4	0.0	0.004353712323607989	0.0
30-31	9.194746195581814E-6	5.838663834194452E-4	0.0	0.01464263331646404	0.0
32-33	9.194746195581814E-6	5.884637565172361E-4	0.0	0.04479680346487459	0.0
34-35	9.194746195581814E-6	6.620217260818906E-4	0.0	0.08412273294337802	0.0
36-37	9.194746195581814E-6	7.769560535266632E-4	0.0	0.13891882289594784	0.0
38-39	9.194746195581814E-6	8.643061423846905E-4	0.0	0.20085463326938693	0.0
40-41	9.194746195581814E-6	9.608509774382995E-4	0.0	0.2568920139583603	0.0
42-43	9.194746195581814E-6	0.0010022273353184178	0.0	0.3609489566537597	0.0
44-45	9.194746195581814E-6	0.001084980051078654	0.0	0.46000395741876254	0.0
46-47	9.194746195581814E-6	0.001140148528252145	0.0	0.5628701804818341	0.0
48-49	9.194746195581814E-6	0.001140148528252145	0.0	0.6295642720114868	0.0
50-51	9.194746195581814E-6	0.0012458881095013356	0.0	0.9626163687078513	0.0
52-53	9.194746195581814E-6	0.0012504854825991266	0.0	1.4150944263655303	0.0
54-55	9.194746195581814E-6	0.0012550828556969176	0.0	2.0245911648051598	0.0
56-57	9.194746195581814E-6	0.0012688749749902902	0.0	2.6903735420810433	0.0
58-59	9.194746195581814E-6	0.0012918618404792448	0.0	3.3735064054279897	0.0
60-61	9.194746195581814E-6	0.0013102513328704084	0.0	4.046281387185614	0.0
62-63	9.194746195581814E-6	0.0013608224369461084	0.0	4.538370411453858	0.0
64-65	9.194746195581814E-6	0.0014619646450975085	0.0	4.545441171278261	0.0
66-67	9.194746195581814E-6	0.0014619646450975085	0.0	4.54893977220568	0.0
68-69	9.194746195581814E-6	0.0015171331222709993	0.0	4.552305049313262	0.0
70-71	9.194746195581814E-6	0.0015723015994444903	0.0	4.555514015735521	0.0
72-73	9.194746195581814E-6	0.0015998858380312357	0.0	4.55838737392164	0.0
74-75	9.194746195581814E-6	0.0016412621959113539	0.0	4.560869955394447	0.0
76-77	9.194746195581814E-6	0.00172401491167159	0.0	4.563141057704756	0.0
78-79	9.194746195581814E-6	0.0017515991502583357	0.0	4.5648788647357215	0.0
80-81	9.194746195581814E-6	0.0017561965233561265	0.0	4.565710989266421	0.0
82-83	9.194746195581814E-6	0.0017699886426494993	0.0	4.5661707265762	0.0
84-85	9.194746195581814E-6	0.0017975728812362445	0.0	4.566363816246307	0.0
86-87	9.194746195581814E-6	0.0018205597467251993	0.0	4.566455763708263	0.0
88	9.194746195581814E-6	0.0019125072086810173	0.0	4.566469555827557	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7745	0.0	34.937355	1
GTATCAA	12985	0.0	30.207697	1
TCAACGC	13240	0.0	28.350616	4
ATCAACG	13370	0.0	28.042576	3
CAACGCA	13720	0.0	27.358759	5
AACGCAG	14385	0.0	26.154072	6
TATCAAC	15370	0.0	24.928768	2
ACGCAGA	17385	0.0	21.491438	7
TGTAGAT	16055	0.0	21.199747	94
CGCAGAG	17955	0.0	20.833284	8
GTACATG	31010	0.0	20.006716	1
GTGTAGA	16355	0.0	19.70727	94
TACATGG	30375	0.0	19.683794	2
GGTGTAG	17565	0.0	19.597475	94
ACATGGG	30525	0.0	18.863722	3
CATGGGG	22600	0.0	17.030396	4
AGGTGTA	21610	0.0	16.943403	94
GCAGAGT	23430	0.0	15.891158	9
AGAGTAC	19445	0.0	15.340541	10-11
TAGGTGT	23440	0.0	14.768068	94
>>END_MODULE