FastQCFastQC Report
Fri 27 May 2016
SRR2139266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9439132
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.034.884011
GTATCAA108400.031.2142581
TCAACGC111100.028.224354
ATCAACG111550.028.1104873
CAACGCA115050.027.1797185
AACGCAG121350.025.8761776
TATCAAC129450.024.9961762
ACGCAGA146850.021.2644027
CGCAGAG150600.020.763798
GTACATG257000.020.5781
TACATGG258400.019.8269312
ACATGGG261150.018.8187643
CATGGGG193500.017.1043434
GTGTAGA133950.016.57388994
GCAGAGT198600.015.5701599
AGAGTAC164700.015.157269510-11
GTATAAG42800.014.3277521
GTGTAGC55550.014.1708941
CAGAGTA189300.014.03761510-11
GTACTAG23450.013.9098071
Produced by FastQC (version 0.11.5)