##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139266_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9439132
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83487401172057	33.0	31.0	34.0	30.0	34.0
2	31.945181717980002	33.0	31.0	34.0	30.0	34.0
3	31.99706848044926	34.0	31.0	34.0	30.0	34.0
4	35.49695046112291	37.0	35.0	37.0	33.0	37.0
5	35.53737017344391	37.0	35.0	37.0	33.0	37.0
6	35.62152664037329	37.0	35.0	37.0	33.0	37.0
7	35.55089673499639	37.0	35.0	37.0	33.0	37.0
8	35.52162857771244	37.0	35.0	37.0	33.0	37.0
9	37.117445332897134	39.0	37.0	39.0	33.0	39.0
10-11	37.0960985607575	39.0	37.0	39.0	33.0	39.0
12-13	37.043204661191304	39.0	37.0	39.0	33.0	39.0
14-15	38.413773798268736	40.0	38.0	41.0	33.0	41.0
16-17	38.420966302833776	40.0	38.0	41.0	33.0	41.0
18-19	38.432420216180894	40.0	38.0	41.0	33.0	41.0
20-21	38.48510377703276	40.0	38.0	41.0	33.0	41.0
22-23	38.46707136133617	40.0	38.0	41.0	33.0	41.0
24-25	38.47338261960178	40.0	38.0	41.0	33.0	41.0
26-27	38.45009283961559	40.0	38.0	41.0	33.0	41.0
28-29	38.423171599317705	40.0	38.0	41.0	33.0	41.0
30-31	38.373014499817046	40.0	38.0	41.0	33.0	41.0
32-33	38.3296060978926	40.0	38.0	41.0	33.0	41.0
34-35	38.27284812338034	40.0	38.0	41.0	33.0	41.0
36-37	38.214821832361594	40.0	38.0	41.0	33.0	41.0
38-39	38.13558999914328	40.0	38.0	41.0	33.0	41.0
40-41	38.06779784094292	40.0	38.0	41.0	33.0	41.0
42-43	37.9850257079286	40.0	37.5	41.0	32.0	41.0
44-45	37.86550647968376	40.0	37.0	41.0	32.0	41.0
46-47	37.76339643376879	40.0	37.0	41.0	32.0	41.0
48-49	37.66126718637297	40.0	37.0	41.0	32.0	41.0
50-51	37.52802728159087	40.0	36.5	41.0	31.0	41.0
52-53	37.40573323310387	40.0	36.0	41.0	31.0	41.0
54-55	37.2667282358083	39.0	36.0	41.0	31.0	41.0
56-57	37.09073833945834	39.0	35.5	41.0	31.0	41.0
58-59	37.24256158171613	39.0	35.5	41.0	31.0	41.0
60-61	37.30965184278793	39.0	35.5	41.0	31.0	41.0
62-63	37.155135633389335	39.0	35.0	41.0	31.0	41.0
64-65	36.95085086077271	39.0	35.0	40.5	31.0	41.0
66-67	36.68526542956144	38.0	35.0	40.0	31.0	41.0
68-69	36.40694314789957	37.5	35.0	40.0	31.0	41.0
70-71	36.16723839384689	37.0	35.0	40.0	31.0	41.0
72-73	35.925748090369055	36.5	34.5	39.0	31.0	41.0
74-75	35.649410371987344	36.0	34.0	39.0	30.5	41.0
76-77	35.3949048291511	36.0	34.0	39.0	30.0	40.5
78-79	35.06481634465966	35.0	34.0	37.5	30.0	39.5
80-81	34.76665291407966	35.0	34.0	37.0	30.0	39.0
82-83	34.48408899265492	35.0	34.0	36.5	30.0	39.0
84-85	34.25409079414074	35.0	34.0	36.0	30.0	37.5
86-87	34.02759792764088	35.0	34.0	36.0	30.0	37.0
88-89	33.86603137723806	35.0	34.0	35.5	30.0	37.0
90-91	33.73820982648641	35.0	34.0	35.0	30.0	36.0
92-93	33.641414524454504	35.0	34.0	35.0	30.0	36.0
94-95	33.60310631050338	35.0	34.0	35.0	30.0	36.0
96-97	33.6057069319893	35.0	34.0	35.0	30.0	36.0
98-99	33.649147016303054	35.0	34.0	35.0	30.0	36.0
100	33.30446019624324	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	1.0
16	39.0
17	357.0
18	1208.0
19	3797.0
20	9516.0
21	19487.0
22	32642.0
23	46239.0
24	61165.0
25	77087.0
26	89650.0
27	98123.0
28	112673.0
29	138824.0
30	164280.0
31	192367.0
32	242136.0
33	316987.0
34	429817.0
35	602348.0
36	929935.0
37	1699662.0
38	2566095.0
39	1521429.0
40	83265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.92581817904443	20.544855183718163	14.399385451967406	24.129941185270003
2	13.979950698856634	21.018182604078426	42.09832005739511	22.903546639669834
3	17.753242565100265	26.1161937347629	30.484307243505015	25.646256456631818
4	12.231188206712227	18.143384370512035	39.58159500259134	30.043832420184398
5	13.302871454118604	38.03242251396316	35.753046894202	12.911659137716228
6	29.736134636108492	37.96178504548936	19.398573936671294	12.903506381730862
7	26.402014507266138	32.656858702685795	23.516516137288896	17.424610652759174
8	24.07831567563628	36.93451897907562	21.640835195439582	17.346330149848523
9	25.160237191301064	17.60226469976265	21.630516450029518	35.606981658906776
10-11	23.63672383914721	27.10055563927916	29.482311792059168	19.78040872951446
12-13	24.335254680718467	24.65026277601546	29.271211386439788	21.74327115682628
14-15	21.721101415066855	26.29285749851946	26.56258044968127	25.423460636732408
16-17	21.674600223781347	29.291795017641732	27.275675832011753	21.75792892656517
18-19	21.697685838253935	28.98365885144222	28.258851124397516	21.059804185906323
20-21	22.605642390539984	28.160741879073036	27.931716149703067	21.301899580683912
22-23	22.33299443026633	28.008641497899234	27.740867064370438	21.917497007464
24-25	22.05640699096822	28.159401403082256	27.813245089273135	21.97094651667639
26-27	21.987111422134863	28.32755050099468	27.733238071686728	21.952100005183727
28-29	21.90007526634055	28.39236073228045	27.762890397618527	21.944673603760467
30-31	22.260143893136256	28.122758009820682	27.717823931420853	21.899274165622213
32-33	22.029649954156895	28.25054857285703	27.67290467520694	22.04689679777913
34-35	22.16052139547878	28.220142167857208	27.73113075762661	21.888205679037398
36-37	22.09623164887896	28.007304023715346	27.842607551904663	22.05385677550103
38-39	21.92066671004872	28.079798866587318	28.000941487458686	21.998592935905275
40-41	22.137630816767402	27.92333803189475	27.98670454709485	21.952326604242998
42-43	21.888978784444042	27.93174667015447	27.970414899029162	22.20885964637232
44-45	21.964888022846885	27.98053868752492	27.9864897592519	22.0680835303763
46-47	21.841700277825215	27.92662530271136	27.947220167210133	22.284454252253287
48-49	21.74798953931745	27.92359201573294	28.124279614794517	22.20413883015509
50-51	21.596994660647244	27.98468644966877	28.132404961481328	22.285913928202657
52-53	21.783197850943324	27.854120786853688	28.052122189025745	22.310559173177246
54-55	21.814582565597977	27.67843949889357	28.102613282133177	22.404364653375275
56-57	21.702963227617744	27.55153602284388	28.155240574260255	22.590260175278125
58-59	21.602004131077987	27.442421704907048	28.310802210684976	22.644771953329982
60-61	21.594978911769456	27.283175095173306	28.576381137050905	22.54546485600633
62-63	21.544758864876044	27.47051495797433	28.502030494887354	22.48269568226228
64-65	21.478305719434957	27.75533548690995	28.150491938299922	22.615866855355176
66-67	21.299270052126882	27.935355233198834	27.96797777139353	22.79739694328075
68-69	21.362933394721786	28.096698826685078	27.934687829665982	22.605679948927147
70-71	21.411199256253116	28.112460438700726	27.661541716750754	22.814798588295403
72-73	21.653598649769286	27.962839880864216	27.35594598699771	23.02761548236879
74-75	21.846240483887787	27.847062854759173	27.427993385277773	22.878703276075267
76-77	21.98214182400247	27.47762906678495	27.579660289665014	22.960568819547568
78-79	22.286914979004337	27.23560587178268	27.520977057454754	22.956502091758225
80-81	22.572361676885983	27.232318185095323	27.183048741287724	23.01227139673097
82-83	22.357189687202027	27.286687292396127	27.312840780767054	23.043282239634795
84-85	22.477822691215586	27.432130286517864	27.294426975367912	22.79562004689863
86-87	22.18814989626726	27.741102055032375	27.4465536493235	22.624194399376865
88-89	21.954247817507706	27.869318528230004	27.43245165085366	22.74398200340863
90-91	21.827102979159648	28.02632816870272	27.695961479421467	22.450607372716167
92-93	21.753418917902508	27.646925775921027	28.191569734703627	22.40808557147284
94-95	21.452532949500295	27.65369454252063	28.5121144278624	22.38165808011668
96-97	21.87184289782783	27.492108822244177	28.498125301861737	22.137922978066257
98-99	23.19966099096065	29.482153012538532	28.801686196175925	18.516499800324894
100	22.30228149944168	30.76664378596246	22.53323427636509	24.397840438230766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3016.0
1	2208.0
2	1558.5
3	1678.5
4	1642.0
5	1709.0
6	1931.5
7	2234.5
8	2525.5
9	2903.5
10	3346.0
11	3706.0
12	4075.0
13	4592.0
14	5316.5
15	6289.0
16	7462.0
17	9030.5
18	11211.5
19	13823.5
20	17121.5
21	21731.0
22	28291.5
23	36925.0
24	47858.5
25	61821.0
26	79123.5
27	98999.0
28	119340.0
29	141319.0
30	162320.0
31	183194.5
32	205387.0
33	226211.0
34	248189.5
35	268108.5
36	289193.0
37	310682.5
38	326814.0
39	339589.0
40	353216.0
41	366442.0
42	375687.0
43	385288.0
44	392855.5
45	398526.5
46	402711.5
47	402868.0
48	398179.0
49	388048.0
50	378079.0
51	364095.5
52	343773.5
53	321999.5
54	299530.0
55	275111.5
56	249965.5
57	224506.0
58	199145.0
59	174004.5
60	146476.5
61	120239.5
62	96255.5
63	75156.5
64	57640.5
65	43676.5
66	33563.5
67	26561.0
68	21364.0
69	16679.5
70	12927.5
71	10082.0
72	7862.5
73	5960.5
74	4514.5
75	3495.5
76	2643.5
77	1962.0
78	1510.0
79	1188.0
80	913.0
81	684.0
82	526.0
83	368.0
84	262.0
85	208.5
86	173.5
87	142.5
88	95.5
89	61.0
90	41.5
91	33.5
92	24.5
93	14.5
94	12.0
95	11.5
96	10.0
97	6.5
98	4.0
99	5.5
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.2713033359423303E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	4.7673875097837384E-5
12-13	1.006448474287678E-4
14-15	2.118838893237217E-5
16-17	7.945645849639564E-5
18-19	8.157529738963287E-4
20-21	4.981675648080229E-4
22-23	0.0
24-25	1.3266946241909284E-4
26-27	1.4340213927311368E-4
28-29	0.0
30-31	0.0
32-33	1.0135777812654338E-4
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	7.752793109849105E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.9069433117002534E-4
76-77	1.807995353210876E-5
78-79	4.391995880307864E-4
80-81	3.0889923879813976E-5
82-83	3.1291553226963334E-5
84-85	4.3747349798229615E-4
86-87	8.998153900182321E-5
88-89	2.608276570171084E-5
90-91	1.986251695431343E-5
92-93	6.525701205095187E-4
94-95	4.1087625044194877E-4
96-97	4.2071145673024736E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3102.0
20-21	5982.0
22-23	6449.0
24-25	7326.0
26-27	9436.0
28-29	12736.0
30-31	17066.0
32-33	18838.0
34-35	23048.0
36-37	35157.0
38-39	40690.0
40-41	34980.0
42-43	38007.0
44-45	45984.0
46-47	59354.0
48-49	36962.0
50-51	37748.0
52-53	15063.0
54-55	15386.0
56-57	15498.0
58-59	15575.0
60-61	17014.0
62-63	77199.0
64-65	83111.0
66-67	87889.0
68-69	88625.0
70-71	89121.0
72-73	88990.0
74-75	92140.0
76-77	98276.0
78-79	102881.0
80-81	104271.0
82-83	102921.0
84-85	105904.0
86-87	110180.0
88-89	114837.0
90-91	118261.0
92-93	127402.0
94-95	148521.0
96-97	337255.0
98-99	620535.0
100-101	6229412.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.30048041578011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.27612902486108	73.53443957016319
2	8.017026510216564	13.356443196141695
3	2.095918452014954	5.237730418954316
4	0.7559438368869204	2.5188193912011436
5	0.3430975044308715	1.4290093474273422
6	0.18326751354978998	0.9159763153981801
7	0.10197923562504853	0.5946443524000407
8	0.06674551043388378	0.4447946467791188
9	0.03785815398737778	0.2838242173322836
>10	0.1201387127482526	1.4835968544901852
>50	0.001436504616363613	0.0784342764202118
>100	3.7670061920762386E-4	0.0603007052375957
>500	6.175595104145716E-5	0.034997928561804614
>1k	2.0584058661446883E-5	0.02698877949287667
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.178258339855826E-5	0.0	0.0	0.0
3	0.0	3.178258339855826E-5	0.0	0.0	0.0
4	0.0	6.356516679711652E-5	0.0	2.1188388932372173E-5	0.0
5	0.0	7.41593612633026E-5	0.0	5.297097233093043E-5	0.0
6	1.0594194466186086E-4	7.41593612633026E-5	0.0	5.297097233093043E-5	0.0
7	1.0594194466186086E-4	7.41593612633026E-5	0.0	6.356516679711652E-5	0.0
8	1.0594194466186086E-4	1.0594194466186086E-4	0.0	6.356516679711652E-5	0.0
9	1.0594194466186086E-4	1.9069550039134953E-4	0.0	7.41593612633026E-5	0.0
10-11	1.0594194466186086E-4	1.9069550039134953E-4	0.0	9.005065296258173E-5	0.0
12-13	1.0594194466186086E-4	1.9069550039134953E-4	0.0	9.534775019567477E-5	0.0
14-15	1.0594194466186086E-4	2.6485486165465217E-4	0.0	1.2183323636113998E-4	0.0
16-17	1.483187225266052E-4	2.966374450532104E-4	0.0	1.853984031582565E-4	0.0
18-19	1.483187225266052E-4	3.1252873675248954E-4	0.0	2.118838893237217E-4	0.0
20-21	1.483187225266052E-4	3.3901422291795477E-4	0.0	3.0723163951939646E-4	0.0
22-23	1.5361581975969823E-4	4.025793897150712E-4	0.0	5.667894039409556E-4	0.0
24-25	1.5891291699279128E-4	4.608474592790947E-4	0.0	0.0012395207525437721	0.0
26-27	1.5891291699279128E-4	4.926300426776531E-4	0.0	0.0030564251034946857	1.0594194466186087E-5
28-29	1.5891291699279128E-4	4.979271399107461E-4	0.0	0.014858357738825986	1.0594194466186087E-5
30-31	1.6421001422588433E-4	5.879777928733277E-4	0.0	0.050735597298565166	1.0594194466186087E-5
32-33	1.6950711145897738E-4	5.932748901064208E-4	0.0	0.14606745620254066	1.0594194466186087E-5
34-35	1.8010130592516346E-4	6.409487652042582E-4	0.0	0.2804707042978104	1.0594194466186087E-5
36-37	1.8010130592516346E-4	6.939197375351886E-4	0.0	0.46844349671135016	1.0594194466186087E-5
38-39	1.8010130592516346E-4	7.309994181668399E-4	0.0	0.6795010388667093	1.0594194466186087E-5
40-41	1.8010130592516346E-4	7.839703904977703E-4	0.0	0.8812833637669226	1.0594194466186087E-5
42-43	1.8010130592516346E-4	8.157529738963286E-4	0.0	1.1885467858697176	1.0594194466186087E-5
44-45	1.8539840315825648E-4	9.111007240920033E-4	0.0	1.4607381271922038	1.0594194466186087E-5
46-47	1.9069550039134953E-4	9.85260085355306E-4	0.0	1.7316104913036496	1.0594194466186087E-5
48-49	1.9599259762444259E-4	9.85260085355306E-4	0.0	1.8885264026395647	1.0594194466186087E-5
50-51	2.1718098655681476E-4	0.0011971439746790277	0.0	2.2725341694554118	1.0594194466186087E-5
52-53	2.224780837899078E-4	0.0011971439746790277	0.0	2.754352836680322	1.0594194466186087E-5
54-55	2.224780837899078E-4	0.0012024410719121206	0.0	3.37582947245573	1.0594194466186087E-5
56-57	2.224780837899078E-4	0.0012077381691452138	0.0	4.048338342974756	1.0594194466186087E-5
58-59	2.224780837899078E-4	0.0012236294608444927	0.0	4.736727910998596	1.0594194466186087E-5
60-61	2.5955776442155914E-4	0.0012395207525437721	0.0	5.407488739430702	1.0594194466186087E-5
62-63	2.966374450532104E-4	0.0012501149470099582	0.0	5.897825139006425	1.0594194466186087E-5
64-65	2.966374450532104E-4	0.0013878394750703772	0.0	5.907243377886864	1.0594194466186087E-5
66-67	3.1782583398558257E-4	0.0014037307667696562	0.0	5.910850701102602	1.0594194466186087E-5
68-69	3.1782583398558257E-4	0.0014514046418674937	0.0	5.914092524609254	1.0594194466186087E-5
70-71	3.2842002845176867E-4	0.0014831872252660519	0.0	5.917366130699306	1.0594194466186087E-5
72-73	3.3901422291795477E-4	0.0014937814197322381	0.0	5.919861063496093	1.0594194466186087E-5
74-75	3.3901422291795477E-4	0.001509672711431517	0.0	5.922287134028849	1.0594194466186087E-5
76-77	3.443113201510478E-4	0.0015255640031307965	0.0	5.924723798756072	1.0594194466186087E-5
78-79	3.496084173841408E-4	0.0015361581975969825	0.0	5.92638179019003	1.0594194466186087E-5
80-81	3.496084173841408E-4	0.0015361581975969825	0.0	5.927213434455625	1.0594194466186087E-5
82-83	3.496084173841408E-4	0.0015414552948300755	0.0	5.927668984817672	1.0594194466186087E-5
84-85	3.496084173841408E-4	0.0015467523920631686	0.0	5.927864977415297	1.0594194466186087E-5
86-87	3.496084173841408E-4	0.0015732378782286338	0.0	5.927949730971026	1.0594194466186087E-5
88	3.496084173841408E-4	0.0016738827256574016	0.0	5.9279603251654915	1.0594194466186087E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6695	0.0	34.88401	1
GTATCAA	10840	0.0	31.214258	1
TCAACGC	11110	0.0	28.22435	4
ATCAACG	11155	0.0	28.110487	3
CAACGCA	11505	0.0	27.179718	5
AACGCAG	12135	0.0	25.876177	6
TATCAAC	12945	0.0	24.996176	2
ACGCAGA	14685	0.0	21.264402	7
CGCAGAG	15060	0.0	20.76379	8
GTACATG	25700	0.0	20.578	1
TACATGG	25840	0.0	19.826931	2
ACATGGG	26115	0.0	18.818764	3
CATGGGG	19350	0.0	17.104343	4
GTGTAGA	13395	0.0	16.573889	94
GCAGAGT	19860	0.0	15.570159	9
AGAGTAC	16470	0.0	15.1572695	10-11
GTATAAG	4280	0.0	14.327752	1
GTGTAGC	5555	0.0	14.170894	1
CAGAGTA	18930	0.0	14.037615	10-11
GTACTAG	2345	0.0	13.909807	1
>>END_MODULE