FastQCFastQC Report
Fri 27 May 2016
SRR2139265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9111195
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.031.8995341
GTATCAA105550.030.5846311
ATCAACG107500.028.1629053
TCAACGC108050.027.861474
CAACGCA111000.027.1210065
AACGCAG119150.025.5167666
TATCAAC127000.024.5447622
ACGCAGA143100.021.126787
CGCAGAG145650.020.6982638
GTACATG252250.020.3137321
TACATGG247100.019.7002532
ACATGGG248500.018.9362893
CATGGGG185650.016.6066464
GTACTAG26600.016.374131
GCAGAGT191550.015.6715829
AGAGTAC157450.015.07897410-11
GTATTAT47700.013.9651581
CAGAGTA186200.013.73683910-11
CGACGAG9400.013.69578624-25
GTATATA88050.013.4335281
Produced by FastQC (version 0.11.5)