##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139265_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9111195
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82280721683599	33.0	31.0	34.0	30.0	34.0
2	31.92461263313978	33.0	31.0	34.0	30.0	34.0
3	31.980147170596172	34.0	31.0	34.0	30.0	34.0
4	35.48325768463961	37.0	35.0	37.0	33.0	37.0
5	35.52743454618192	37.0	35.0	37.0	33.0	37.0
6	35.609201098209404	37.0	35.0	37.0	33.0	37.0
7	35.53749744133454	37.0	35.0	37.0	33.0	37.0
8	35.509130580565994	37.0	35.0	37.0	33.0	37.0
9	37.09604832296971	39.0	37.0	39.0	33.0	39.0
10-11	37.075637059683174	39.0	37.0	39.0	33.0	39.0
12-13	37.02066441339473	39.0	37.0	39.0	33.0	39.0
14-15	38.38362207152849	40.0	38.0	41.0	33.0	41.0
16-17	38.38845277705065	40.0	38.0	41.0	33.0	41.0
18-19	38.401135032232325	40.0	38.0	41.0	33.0	41.0
20-21	38.45399484297181	40.0	38.0	41.0	33.0	41.0
22-23	38.433024284143904	40.0	38.0	41.0	33.0	41.0
24-25	38.442231743970616	40.0	38.0	41.0	33.0	41.0
26-27	38.41680334806982	40.0	38.0	41.0	33.0	41.0
28-29	38.38942046545254	40.0	38.0	41.0	33.0	41.0
30-31	38.34046240986494	40.0	38.0	41.0	33.0	41.0
32-33	38.29735254856644	40.0	38.0	41.0	33.0	41.0
34-35	38.242526972587925	40.0	38.0	41.0	33.0	41.0
36-37	38.18609508350957	40.0	38.0	41.0	33.0	41.0
38-39	38.10823642879177	40.0	38.0	41.0	33.0	41.0
40-41	38.04211047824757	40.0	38.0	41.0	32.5	41.0
42-43	37.96064343655011	40.0	37.0	41.0	32.0	41.0
44-45	37.84672694328111	40.0	37.0	41.0	32.0	41.0
46-47	37.74764528684837	40.0	37.0	41.0	32.0	41.0
48-49	37.64689235138445	40.0	37.0	41.0	32.0	41.0
50-51	37.50889646025442	40.0	36.0	41.0	31.0	41.0
52-53	37.38210845246222	40.0	36.0	41.0	31.0	41.0
54-55	37.2381604642407	39.0	36.0	41.0	31.0	41.0
56-57	37.05841942628905	39.0	35.0	41.0	31.0	41.0
58-59	37.201896795857465	39.0	35.5	41.0	31.0	41.0
60-61	37.26409296504731	39.0	35.0	41.0	31.0	41.0
62-63	37.10772250196051	39.0	35.0	41.0	31.0	41.0
64-65	36.9058633040824	38.5	35.0	40.5	31.0	41.0
66-67	36.639288053676324	38.0	35.0	40.0	31.0	41.0
68-69	36.36631586660359	37.5	35.0	40.0	31.0	41.0
70-71	36.13438342645762	37.0	35.0	40.0	31.0	41.0
72-73	35.901713398346196	36.5	34.5	39.0	30.5	41.0
74-75	35.63512145888494	36.0	34.0	39.0	30.0	41.0
76-77	35.38610812368509	36.0	34.0	39.0	30.0	40.5
78-79	35.085616209659975	35.0	34.0	37.5	30.0	39.5
80-81	34.79870859679859	35.0	34.0	37.0	30.0	39.0
82-83	34.534002299172684	35.0	34.0	37.0	30.0	39.0
84-85	34.30936131683002	35.0	34.0	36.0	30.0	37.5
86-87	34.11938056709839	35.0	34.0	36.0	30.0	37.0
88-89	33.97756493473722	35.0	34.0	36.0	30.0	37.0
90-91	33.86609929297839	35.0	34.0	35.0	30.0	36.0
92-93	33.77909084594779	35.0	34.0	35.0	30.0	36.0
94-95	33.75290107935969	35.0	34.0	35.0	30.0	36.0
96-97	33.75417122835306	35.0	34.0	35.0	30.0	36.0
98-99	33.78672529710729	35.0	34.0	35.0	30.0	36.0
100	33.45494896971752	35.0	33.0	35.0	30.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	0.0
16	21.0
17	274.0
18	1119.0
19	3733.0
20	9453.0
21	19363.0
22	31899.0
23	45309.0
24	59953.0
25	74721.0
26	87366.0
27	96551.0
28	110174.0
29	134897.0
30	160251.0
31	185862.0
32	231650.0
33	299420.0
34	403954.0
35	565359.0
36	860856.0
37	1576048.0
38	2415563.0
39	1644011.0
40	93385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.863717657233764	20.73507371974807	14.391811392468275	24.00939723054989
2	13.949103273500349	21.042047722609382	42.33226267245954	22.67658633143073
3	17.644557053163716	26.401333743817357	30.583639138444518	25.37047006457441
4	12.162553869168644	18.303230256843367	39.90216431543831	29.632051558549676
5	13.169179018794816	38.1567574704624	35.916642218688835	12.757421292053953
6	29.31252157373429	38.29305596027744	19.594443977985325	12.799978488002948
7	26.10608158424883	32.777445768639566	23.84641092633842	17.27006172077318
8	23.907791218998433	37.07285094279092	21.744089934216078	17.27526790399457
9	25.060181458085356	17.629290120560476	21.83094533702769	35.47958308432648
10-11	23.566079656088945	27.18809161434732	29.607033897756306	19.638794831807427
12-13	24.247869596779168	24.77187382630538	29.35360445939749	21.62665211751796
14-15	21.649182313536514	26.351002953070697	26.685141376640647	25.31467335675214
16-17	21.574225932531967	29.395856018352816	27.369836143230042	21.66008190588517
18-19	21.578185920667263	29.096603140354027	28.353702154617554	20.971508784361152
20-21	22.511285057220977	28.264722019215547	28.02068576230612	21.203307161257356
22-23	22.236924564676823	28.152756489597785	27.82292895530844	21.78738999041695
24-25	21.975888043075546	28.274056057960532	27.909325970375498	21.84072992858842
26-27	21.92887095294752	28.450446804971524	27.758187013369177	21.862495228711772
28-29	21.79903723452218	28.495662553856256	27.85837973531413	21.846920476307435
30-31	22.149210113848174	28.262300113654547	27.78503154750849	21.803458224988788
32-33	21.959347328168764	28.38968724658534	27.734269957428825	21.91669546781708
34-35	22.07342170906014	28.323931228206977	27.84926429865797	21.753382764074914
36-37	21.9835867827895	28.133700465727955	27.93217565929995	21.95053709218259
38-39	21.83392102843473	28.203074009651445	28.096564438931644	21.86644052298218
40-41	22.007853558322847	28.00887688311316	28.118952654195446	21.86431690436855
42-43	21.822773549593528	28.050377172238335	28.07545785179985	22.051391426368287
44-45	21.87497489806777	28.153331596326765	28.043279068659764	21.9284144369457
46-47	21.75298740819848	28.09441497091141	27.968594305750994	22.184003315139122
48-49	21.700211786370424	28.09507896943513	28.11078680731946	22.093922436874987
50-51	21.635842274711546	28.1961296053359	28.08001730373385	22.088010816218695
52-53	21.7752064072596	28.1296015351549	27.9941730007025	22.101019056883008
54-55	21.823461379778813	28.00911057491817	27.97462598904845	22.19280205625456
56-57	21.847714494902043	28.069517735314903	27.880911081485298	22.201856688297752
58-59	21.810458911749368	28.0954469470222	27.965749054822524	22.128345086405908
60-61	21.899924260568834	28.011893016119053	27.969341695561557	22.118841027750552
62-63	21.88423306810159	28.093538881175604	27.784567160605867	22.237660890116935
64-65	21.956950104777658	28.117243738715043	27.682444242859432	22.24336191364787
66-67	22.00933319485912	28.143810733050746	27.587800586278767	22.25905548581137
68-69	21.968422070579216	28.037783999355963	27.6980889043196	22.29570502574522
70-71	22.028795476907064	27.905725912163838	27.806887778136126	22.258590832792976
72-73	22.098087662981893	27.92044374578318	27.752413760538307	22.22905483069662
74-75	22.062525368244458	28.09100136538977	27.754501594777565	22.091971671588215
76-77	22.098464072106097	28.075139242562635	27.722003520488304	22.104393164842968
78-79	22.126272955378756	28.01937337410042	27.72662412002871	22.127729550492113
80-81	22.067338636091836	28.121774209218643	27.74298094301548	22.06790621167404
82-83	22.127003599859325	28.010601066764313	27.927861000477538	21.934534332898828
84-85	22.191366282366566	28.101469926966477	27.81311800350268	21.894045787164274
86-87	21.92623714180392	28.192371568159214	27.91655384499052	21.964837445046346
88-89	22.086915143310925	28.138487287029484	27.87273047104266	21.90186709861693
90-91	22.06819334463399	28.1689038102502	27.894857981596378	21.86804486351943
92-93	22.023102162664845	28.157323146613912	27.92959398970392	21.88998070101733
94-95	21.844584253174517	28.291368218446213	27.959993057997217	21.904054470382054
96-97	21.952046455109627	28.407983356880745	27.982728514391248	21.657241673618376
98-99	22.932418769991916	30.368100364336602	28.20515501254015	18.49432585313133
100	21.89491135854104	31.368166489400878	21.996817779330637	24.740104372727444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2213.0
1	1689.0
2	1277.5
3	1387.5
4	1382.5
5	1478.0
6	1718.5
7	2007.5
8	2276.5
9	2596.0
10	2974.5
11	3357.5
12	3817.0
13	4390.5
14	5063.0
15	6004.0
16	7259.5
17	8860.0
18	11001.5
19	13803.5
20	17427.5
21	22647.5
22	29877.5
23	39032.5
24	50951.0
25	66231.5
26	85401.0
27	106755.0
28	128474.0
29	152252.5
30	174721.0
31	196053.5
32	219550.0
33	241527.0
34	262691.0
35	281683.5
36	301793.5
37	321867.0
38	333782.5
39	341457.5
40	351510.5
41	359520.5
42	363501.0
43	370030.0
44	375828.5
45	382183.0
46	385896.5
47	385301.0
48	382162.5
49	371936.5
50	359951.0
51	347617.5
52	331074.0
53	311538.0
54	290623.0
55	266582.0
56	242634.5
57	217910.0
58	193309.5
59	169909.5
60	143016.5
61	116522.0
62	93426.0
63	74073.5
64	56746.0
65	42510.5
66	32475.0
67	25239.0
68	19823.5
69	15071.0
70	11698.5
71	9263.0
72	7178.5
73	5387.0
74	3980.0
75	3005.0
76	2286.0
77	1775.5
78	1366.0
79	1036.0
80	810.0
81	639.0
82	493.5
83	344.5
84	245.5
85	183.0
86	141.5
87	112.0
88	68.0
89	40.5
90	36.5
91	30.5
92	22.0
93	16.0
94	10.0
95	6.0
96	6.5
97	6.0
98	4.0
99	9.5
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	1.3170610441330693E-4
6	0.0
7	0.0
8	2.195101740221782E-5
9	0.0
10-11	2.195101740221782E-5
12-13	9.87795783099802E-5
14-15	1.097550870110891E-5
16-17	6.585305220665346E-5
18-19	9.713325200481387E-4
20-21	4.556991770512091E-4
22-23	0.0
24-25	8.246705358742129E-5
26-27	1.3205796882628584E-4
28-29	0.0
30-31	0.0
32-33	6.631536470632186E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	3.4625922881837074E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	1.415376352753991E-4
76-77	1.9524999712006254E-5
78-79	3.8886324661660965E-4
80-81	4.0064142692450617E-5
82-83	2.0297665261053347E-5
84-85	3.7711742003413665E-4
86-87	9.034992595823568E-5
88-89	3.523783743136462E-5
90-91	2.1450604263522103E-5
92-93	7.113309946512264E-4
94-95	4.7204215985545476E-4
96-97	4.8219727654978206E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2887.0
20-21	5817.0
22-23	6191.0
24-25	7280.0
26-27	9062.0
28-29	11853.0
30-31	16263.0
32-33	18366.0
34-35	22737.0
36-37	34792.0
38-39	41102.0
40-41	38085.0
42-43	44715.0
44-45	54689.0
46-47	60396.0
48-49	57646.0
50-51	57290.0
52-53	57651.0
54-55	63877.0
56-57	72072.0
58-59	73880.0
60-61	74057.0
62-63	73954.0
64-65	78576.0
66-67	82736.0
68-69	83441.0
70-71	83922.0
72-73	84899.0
74-75	87286.0
76-77	94795.0
78-79	98208.0
80-81	98752.0
82-83	97455.0
84-85	97400.0
86-87	99110.0
88-89	101146.0
90-91	104255.0
92-93	106881.0
94-95	123216.0
96-97	301101.0
98-99	544664.0
100-101	5838690.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.32343424801593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.97722083591083	75.02864830728119
2	7.605690573339004	12.826758979434121
3	1.9254252552294695	4.870754097264343
4	0.7102564931741556	2.395650668055891
5	0.3218597457429139	1.3570159553617864
6	0.16287312639158166	0.8240412818429595
7	0.09169583650664412	0.5412475488339377
8	0.0566684399820153	0.38227819782088834
9	0.04056319885903331	0.3078385408691001
>10	0.1060266639736659	1.3195274396298977
>50	0.0013656246354780384	0.07295804634765057
>100	3.127929610132485E-4	0.04997131871016077
>500	4.141329416554303E-5	0.023309618548138376
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.097550870110891E-5	0.0	0.0	0.0
3	0.0	1.097550870110891E-5	0.0	1.097550870110891E-5	0.0
4	0.0	4.390203480443564E-5	0.0	2.195101740221782E-5	0.0
5	0.0	5.4877543505544556E-5	0.0	3.292652610332673E-5	0.0
6	3.292652610332673E-5	5.4877543505544556E-5	0.0	3.292652610332673E-5	0.0
7	3.292652610332673E-5	8.780406960887128E-5	0.0	5.4877543505544556E-5	0.0
8	4.390203480443564E-5	9.87795783099802E-5	0.0	6.585305220665346E-5	0.0
9	4.390203480443564E-5	1.8658364791885148E-4	0.0	8.780406960887128E-5	2.195101740221782E-5
10-11	4.93897891549901E-5	1.8658364791885148E-4	0.0	9.329182395942574E-5	2.195101740221782E-5
12-13	5.4877543505544556E-5	1.8658364791885148E-4	0.0	1.0426733266053465E-4	2.195101740221782E-5
14-15	5.4877543505544556E-5	2.2499792837273267E-4	0.0	1.481693674649703E-4	6.0365297856099007E-5
16-17	6.585305220665346E-5	2.304856827232871E-4	0.0	1.701203848671881E-4	9.87795783099802E-5
18-19	8.231631525831682E-5	2.6341220882661386E-4	0.0	2.6341220882661386E-4	1.3170610441330693E-4
20-21	1.1524284136164357E-4	3.182897523321584E-4	0.0	4.390203480443564E-4	1.3170610441330693E-4
22-23	1.5365712181552476E-4	3.896305588893663E-4	0.0	7.682856090776237E-4	1.591448761660792E-4
24-25	1.9755915661996037E-4	4.4450810239491084E-4	0.0	0.001432303885494713	2.2499792837273267E-4
26-27	2.304856827232871E-4	4.609713654465742E-4	0.0	0.0030237526471555047	2.688999631771683E-4
28-29	2.4146119142439603E-4	4.609713654465742E-4	0.0	0.012665737041079683	2.853632262288317E-4
30-31	2.5243670012550497E-4	5.487754350554455E-4	0.0	0.04597091819459467	2.853632262288317E-4
32-33	2.688999631771683E-4	5.597509437565544E-4	0.0	0.13508656109324846	3.182897523321584E-4
34-35	2.9633873492994063E-4	6.420672590148713E-4	0.0	0.25538362421175265	3.292652610332673E-4
36-37	3.073142436310495E-4	7.134080655720791E-4	0.0	0.4277539883626681	3.3475301538382174E-4
38-39	3.1280199798160396E-4	7.408468373248515E-4	0.0	0.6111876652843014	3.4024076973437624E-4
40-41	3.6219178713659407E-4	8.012121351809505E-4	0.0	0.7832726662089879	3.512162784354851E-4
42-43	3.7316729583770295E-4	8.121876438820595E-4	0.0	1.0176381912581172	3.567040327860396E-4
44-45	3.786550501882574E-4	8.835284504392673E-4	0.0	1.1908591573333684	3.676795414871485E-4
46-47	4.0060606759047523E-4	9.438937482953663E-4	0.0	1.3490436764881006	3.8414280453881184E-4
48-49	4.0609382194102967E-4	9.438937482953663E-4	0.0	1.460642648960976	3.8414280453881184E-4
50-51	4.1158157629158417E-4	0.0010755998527086732	0.0	1.5944395877818442	3.896305588893663E-4
52-53	4.2255708499269306E-4	0.001086575361409782	0.0	1.7247353393270588	4.2255708499269306E-4
54-55	4.280448393432475E-4	0.001097550870110891	0.0	1.8420799906049645	4.554836110960198E-4
56-57	4.280448393432475E-4	0.0011414529049153267	0.0	1.9370455796413095	4.664591197971287E-4
58-59	4.3353259369380195E-4	0.0011414529049153267	0.0	1.982330528542085	4.7194687414768316E-4
60-61	4.609713654465743E-4	0.00115791616796699	0.0	2.000374264846708	4.8292238284879205E-4
62-63	4.993856459004555E-4	0.0011798671853692079	0.0	2.011799769404562	5.048734002510099E-4
64-65	5.158489089521188E-4	0.0013280365528341783	0.0	2.015953999447932	5.048734002510099E-4
66-67	5.323121720037822E-4	0.0013280365528341783	0.0	2.019746037704165	5.103611546015644E-4
68-69	5.377999263543366E-4	0.0013884018506902772	0.0	2.0235600269778002	5.158489089521188E-4
70-71	5.43287680704891E-4	0.0014158406224430494	0.0	2.0269569469207935	5.487754350554455E-4
72-73	5.542631894059999E-4	0.0014377916398452674	0.0	2.0299642363048975	5.817019611587723E-4
74-75	5.652386981071089E-4	0.0014652304115980396	0.0	2.0328672583563407	5.817019611587723E-4
76-77	5.707264524576634E-4	0.001520107955103584	0.0	2.0355672334968133	5.871897155093267E-4
78-79	5.707264524576634E-4	0.0015475467268563565	0.0	2.0375318495543118	5.926774698598811E-4
80-81	5.707264524576634E-4	0.0015475467268563565	0.0	2.038486718811309	6.036529785609901E-4
82-83	5.707264524576634E-4	0.0015475467268563565	0.0	2.03909585954422	6.256039959632079E-4
84-85	5.707264524576634E-4	0.0015585222355574654	0.0	2.039414149296552	6.256039959632079E-4
86-87	5.707264524576634E-4	0.0015694977442585743	0.0	2.0394415880683052	6.256039959632079E-4
88	5.707264524576634E-4	0.0016573018138674456	0.0	2.0394470758226557	6.365795046643168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6760	0.0	31.899534	1
GTATCAA	10555	0.0	30.584631	1
ATCAACG	10750	0.0	28.162905	3
TCAACGC	10805	0.0	27.86147	4
CAACGCA	11100	0.0	27.121006	5
AACGCAG	11915	0.0	25.516766	6
TATCAAC	12700	0.0	24.544762	2
ACGCAGA	14310	0.0	21.12678	7
CGCAGAG	14565	0.0	20.698263	8
GTACATG	25225	0.0	20.313732	1
TACATGG	24710	0.0	19.700253	2
ACATGGG	24850	0.0	18.936289	3
CATGGGG	18565	0.0	16.606646	4
GTACTAG	2660	0.0	16.37413	1
GCAGAGT	19155	0.0	15.671582	9
AGAGTAC	15745	0.0	15.078974	10-11
GTATTAT	4770	0.0	13.965158	1
CAGAGTA	18620	0.0	13.736839	10-11
CGACGAG	940	0.0	13.695786	24-25
GTATATA	8805	0.0	13.433528	1
>>END_MODULE