FastQCFastQC Report
Fri 27 May 2016
SRR2139264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8232642
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61600.036.883091
GTATCAA98650.029.891171
ATCAACG100000.027.773753
TCAACGC102450.027.1524644
CAACGCA102150.027.1461645
AACGCAG108950.025.5325366
TCGGTGG37850.024.75647594
TATCAAC116050.024.6899342
ACGCAGA132200.020.9756477
GTACATG226800.020.9459511
TACATGG226150.020.5397832
CGCAGAG134850.020.4982688
ACATGGG228900.019.4290773
CATGGGG176700.018.0060944
CTCGGTG49700.016.29504894
GTACTAG22400.015.8911181
TCTCGGT51700.015.8390292-93
GCAGAGT174850.015.6078629
ACCGAAC8300.015.354568
AGAGTAC143100.015.24744510-11
Produced by FastQC (version 0.11.5)