##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139264_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8232642
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.806622952874665	33.0	31.0	34.0	30.0	34.0
2	31.897259105886057	33.0	31.0	34.0	30.0	34.0
3	31.95180672255638	34.0	31.0	34.0	30.0	34.0
4	35.45526709408717	37.0	35.0	37.0	33.0	37.0
5	35.5027654548807	37.0	35.0	37.0	33.0	37.0
6	35.57706857652744	37.0	35.0	37.0	33.0	37.0
7	35.503805582703585	37.0	35.0	37.0	33.0	37.0
8	35.47243582315373	37.0	35.0	37.0	33.0	37.0
9	37.0499045142495	39.0	37.0	39.0	33.0	39.0
10-11	37.0284542313391	39.0	37.0	39.0	33.0	39.0
12-13	36.972792816692376	39.0	37.0	39.0	33.0	39.0
14-15	38.32946561990671	40.0	38.0	41.0	33.0	41.0
16-17	38.33923538761918	40.0	38.0	41.0	33.0	41.0
18-19	38.353760433163494	40.0	38.0	41.0	33.0	41.0
20-21	38.40677389487864	40.0	38.0	41.0	33.0	41.0
22-23	38.39056705291209	40.0	38.0	41.0	33.0	41.0
24-25	38.40044685663978	40.0	38.0	41.0	33.0	41.0
26-27	38.375244131970135	40.0	38.0	41.0	33.0	41.0
28-29	38.34374645005026	40.0	38.0	41.0	33.0	41.0
30-31	38.292947414508745	40.0	38.0	41.0	33.0	41.0
32-33	38.249553640016416	40.0	38.0	41.0	33.0	41.0
34-35	38.19351512744851	40.0	38.0	41.0	33.0	41.0
36-37	38.14149811425725	40.0	38.0	41.0	33.0	41.0
38-39	38.066604990625805	40.0	38.0	41.0	33.0	41.0
40-41	38.00148887890519	40.0	37.5	41.0	32.0	41.0
42-43	37.92230773139069	40.0	37.0	41.0	32.0	41.0
44-45	37.82077909809981	40.0	37.0	41.0	32.0	41.0
46-47	37.72819578267696	40.0	37.0	41.0	32.0	41.0
48-49	37.631633620961054	40.0	37.0	41.0	31.5	41.0
50-51	37.50267380503045	40.0	36.0	41.0	31.0	41.0
52-53	37.385305797634146	40.0	36.0	41.0	31.0	41.0
54-55	37.25090454042015	39.0	36.0	41.0	31.0	41.0
56-57	37.07895596036809	39.0	35.5	41.0	31.0	41.0
58-59	37.23418640934203	39.0	35.5	41.0	31.0	41.0
60-61	37.299287682617404	39.0	35.5	41.0	31.0	41.0
62-63	37.146645190937036	39.0	35.0	41.0	31.0	41.0
64-65	36.94172830718335	39.0	35.0	41.0	31.0	41.0
66-67	36.669417653458886	38.0	35.0	40.0	31.0	41.0
68-69	36.384371590506234	37.5	35.0	40.0	31.0	41.0
70-71	36.13661604206875	37.0	35.0	40.0	30.5	41.0
72-73	35.88129515936707	36.5	34.5	39.0	30.0	41.0
74-75	35.59276562870375	36.0	34.0	39.0	30.0	41.0
76-77	35.31169459226702	36.0	34.0	39.0	30.0	40.5
78-79	34.969022156795035	35.0	34.0	37.0	30.0	39.5
80-81	34.644304861314126	35.0	34.0	37.0	30.0	39.0
82-83	34.35327122659919	35.0	34.0	36.5	30.0	39.0
84-85	34.11177728245871	35.0	34.0	36.0	30.0	37.5
86-87	33.89301750348534	35.0	34.0	36.0	29.0	37.0
88-89	33.72955308034497	35.0	34.0	35.5	29.0	37.0
90-91	33.588794708894156	35.0	33.5	35.0	29.0	36.0
92-93	33.47951313061653	35.0	33.0	35.0	29.0	36.0
94-95	33.454894051320196	35.0	33.0	35.0	29.0	36.0
96-97	33.46935459901534	35.0	33.0	35.0	29.0	36.0
98-99	33.577970410949405	35.0	34.0	35.0	30.0	36.0
100	33.22190513148984	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	44.0
17	288.0
18	1117.0
19	3491.0
20	8662.0
21	17642.0
22	29367.0
23	42084.0
24	55821.0
25	69976.0
26	80842.0
27	89131.0
28	102852.0
29	126154.0
30	149890.0
31	175200.0
32	221376.0
33	288793.0
34	388754.0
35	543071.0
36	840510.0
37	1517071.0
38	2182571.0
39	1222715.0
40	75217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.573803646508615	20.96024581173334	14.347508369731127	24.118442172026914
2	13.912350373063715	21.24763836445214	42.17631472375454	22.663696538729607
3	17.474888377266982	26.52078154254734	30.80487406108513	25.199456019100552
4	12.162887685387023	18.25887728386586	39.94965407216784	29.628580958579278
5	13.08352830940863	38.22033142734148	35.98817826712569	12.707961996124206
6	29.28737821953147	38.434624024705556	19.487522960429956	12.790474795333019
7	25.98872877018095	32.89606179887332	23.930385895560626	17.184823535385117
8	23.860702780553652	37.09317581077567	21.87196065029436	17.174160758376324
9	25.13204145157776	17.4872416412617	21.9274322872293	35.453284619931246
10-11	23.487189223285508	27.200755432128144	29.714329755015374	19.597725589570974
12-13	24.107758004973377	24.874444770518103	29.443864368041716	21.5739328564668
14-15	21.600504625429434	26.436540837867216	26.740592721096423	25.222361815606924
16-17	21.495547901734984	29.451591136981055	27.445562690438724	21.60729827084524
18-19	21.524856284176767	29.16252356798597	28.45612777352772	20.85649237430954
20-21	22.423441895464773	28.347541006716533	28.056636737098618	21.172380360720076
22-23	22.181571921998362	28.182058460916032	27.901176783325955	21.735192833759648
24-25	21.91392283559015	28.314703641662536	27.98044188118481	21.790931641562505
26-27	21.83928855462905	28.5021833497386	27.856233599300822	21.802294496331527
28-29	21.784957703641844	28.538927176331374	27.90895781068805	21.767157309338728
30-31	22.100794161978815	28.315504017509863	27.83514141574895	21.748560404762372
32-33	21.936833417396485	28.380118299784492	27.770639032815197	21.91240925000382
34-35	22.02050431314409	28.344434248320628	27.90988341207007	21.72517802646522
36-37	21.930524306516748	28.117208191240188	27.994140559812625	21.958126942430436
38-39	21.7701541802191	28.11624002239454	28.232709107833276	21.880896689553083
40-41	21.972589549198197	27.82999323151536	28.271048759857447	21.926368459428996
42-43	21.681530502999834	27.89473397984718	28.242790742744393	22.18094477440859
44-45	21.675614340426232	27.900611681935295	28.321526002269653	22.10224797536882
46-47	21.566473735077167	27.777573533172596	28.336504566644166	22.319448165106078
48-49	21.414793317105378	27.73775989722942	28.546160346273158	22.301286439392044
50-51	21.261319307353187	27.795906860830183	28.56146078469915	22.38131304711748
52-53	21.301302276761522	27.7269513636774	28.549456378940484	22.422289980620587
54-55	21.196696326650795	27.61483339123788	28.651117792826376	22.537352489284952
56-57	21.22205415564088	27.67363963431318	28.5067538115194	22.597552398526545
58-59	21.152753908978337	27.67637620938816	28.486927140204028	22.683942741429476
60-61	21.175691460724106	27.493187375156236	28.536249034742255	22.794872129377403
62-63	21.17648803652812	27.446024879838653	28.43809008014611	22.93939700348712
64-65	21.34560447352959	27.52422402090889	28.279493030370567	22.850678475190954
66-67	21.210311749679246	27.691209070942207	27.943860497423085	23.154618681955462
68-69	21.222029918569906	27.790527994725384	27.831936303859163	23.155505782845545
70-71	21.39233210884746	27.74516685474001	27.64840235007725	23.214098686335277
72-73	21.57821936522835	27.69969872472152	27.2201996165011	23.50188229354903
74-75	21.63932560685519	27.6565447946447	27.030768317383668	23.67336128111644
76-77	21.930930424366675	27.400746326959226	27.162491014907886	23.505832233766213
78-79	22.089738924692462	27.137530332816333	27.327470500704987	23.44526024178622
80-81	22.18433239672406	27.065909299004986	27.33238374291391	23.41737456135705
82-83	22.199718304284474	27.18644660342141	27.216990988596457	23.39684410369766
84-85	22.322661395265673	26.977749074518808	27.33782934613479	23.36176018408073
86-87	21.966749044897444	27.22881461007105	27.615610456953814	23.188825888077687
88-89	22.12209213458023	27.397405529587093	27.3768480163255	23.103654319507175
90-91	22.05149640981095	27.635910646122703	27.680809635056747	22.631783309009602
92-93	21.77847294120102	27.579892821613406	27.97513767058848	22.66649656659709
94-95	21.61009862605657	27.634770892656483	28.254696700933877	22.500433780353067
96-97	21.76319849104205	27.504040212685908	28.53830889695371	22.194452399318333
98-99	23.05642723177142	29.603785647735325	28.648809535300295	18.690977585192954
100	22.56648629101275	30.698181163368428	22.383657888105468	24.351674657513353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2437.0
1	1805.5
2	1350.5
3	1518.0
4	1496.5
5	1603.5
6	1855.0
7	2149.5
8	2468.5
9	2853.5
10	3271.0
11	3576.0
12	3901.5
13	4418.5
14	5073.0
15	5917.0
16	7017.0
17	8223.0
18	9640.0
19	11515.5
20	14309.5
21	18392.5
22	23736.5
23	30552.5
24	39666.0
25	51931.5
26	67706.5
27	84901.0
28	102215.0
29	121658.0
30	140048.0
31	157126.5
32	176506.5
33	194508.0
34	211231.5
35	227311.0
36	245642.0
37	265546.0
38	279805.0
39	289643.5
40	302423.0
41	314524.0
42	322230.5
43	330040.5
44	336267.5
45	343895.0
46	347565.5
47	346582.5
48	344071.5
49	336201.5
50	326375.5
51	314764.5
52	298930.5
53	279114.5
54	257993.5
55	235750.0
56	213070.5
57	189458.5
58	165918.5
59	143754.0
60	119462.5
61	96414.5
62	76942.0
63	59709.5
64	44952.5
65	33535.0
66	25841.0
67	20409.0
68	16121.5
69	12478.0
70	9762.5
71	7642.5
72	5897.0
73	4557.0
74	3504.0
75	2623.5
76	1927.0
77	1467.0
78	1162.5
79	905.0
80	683.5
81	513.5
82	397.0
83	287.5
84	209.5
85	161.0
86	134.0
87	105.5
88	72.5
89	49.0
90	34.0
91	28.5
92	25.0
93	15.5
94	8.0
95	6.5
96	6.5
97	6.5
98	4.5
99	3.0
100	16.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	9.717415138420936E-5
6	0.0
7	0.0
8	1.214676892302617E-5
9	0.0
10-11	3.64403067690785E-5
12-13	1.2146768923026167E-4
14-15	1.214676892302617E-5
16-17	8.502738246118318E-5
18-19	9.231544381499888E-4
20-21	3.7673100300497663E-4
22-23	0.0
24-25	1.0343564538366715E-4
26-27	1.4005961302503248E-4
28-29	0.0
30-31	0.0
32-33	6.725047825788982E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	6.842462806393821E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	2.2038332005468178E-4
76-77	2.70329888970108E-5
78-79	4.313069119739562E-4
80-81	2.7767147202602223E-5
82-83	7.747013526285616E-5
84-85	4.8595521536784773E-4
86-87	1.959999886755562E-4
88-89	1.4768326311486451E-5
90-91	1.5044055010693314E-5
92-93	5.98364072625442E-4
94-95	2.9780676290945297E-4
96-97	3.622954459784481E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	2738.0
20-21	5119.0
22-23	5583.0
24-25	6541.0
26-27	8184.0
28-29	10552.0
30-31	12557.0
32-33	12935.0
34-35	14695.0
36-37	19573.0
38-39	21320.0
40-41	18987.0
42-43	12805.0
44-45	12809.0
46-47	13514.0
48-49	13461.0
50-51	13244.0
52-53	13049.0
54-55	13166.0
56-57	13081.0
58-59	13140.0
60-61	14834.0
62-63	44579.0
64-65	78212.0
66-67	82163.0
68-69	82689.0
70-71	81767.0
72-73	83231.0
74-75	86896.0
76-77	93060.0
78-79	99539.0
80-81	103279.0
82-83	102766.0
84-85	106877.0
86-87	114489.0
88-89	121916.0
90-91	128232.0
92-93	135101.0
94-95	151852.0
96-97	308488.0
98-99	546722.0
100-101	5408897.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.32246776839325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.33607319864198	71.89721070913238
2	8.67446730157563	14.282071096838816
3	2.273615865606311	5.615090064422492
4	0.826176598909773	2.720515857390022
5	0.3690263009977648	1.5189577884788938
6	0.18695830511215528	0.92345214279773
7	0.10705072753553421	0.6168876046988945
8	0.060540230252835905	0.39870569229441516
9	0.03942093414631951	0.29207057225943067
>10	0.12441168926760472	1.5313843214841707
>50	0.0017948782346400155	0.09675560994199082
>100	4.1539457521434054E-4	0.06355126925189833
>500	2.4287572254121378E-5	0.017854757054677494
>1k	2.4287571951497417E-5	0.025492513954158393
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2146768923026168E-5	1.2146768923026168E-5
2	0.0	4.858707569210467E-5	0.0	2.4293537846052335E-5	1.2146768923026168E-5
3	0.0	4.858707569210467E-5	0.0	2.4293537846052335E-5	1.2146768923026168E-5
4	0.0	1.3361445815328785E-4	0.0	6.073384461513084E-5	3.6440306769078507E-5
5	0.0	1.3361445815328785E-4	0.0	6.073384461513084E-5	3.6440306769078507E-5
6	0.0	1.3361445815328785E-4	0.0	7.288061353815701E-5	6.073384461513084E-5
7	0.0	1.3361445815328785E-4	0.0	9.717415138420934E-5	7.288061353815701E-5
8	0.0	1.4576122707631403E-4	0.0	9.717415138420934E-5	7.288061353815701E-5
9	0.0	3.5225629876775886E-4	0.0	1.4576122707631403E-4	1.2146768923026168E-4
10-11	0.0	3.5225629876775886E-4	0.0	1.5790799599934018E-4	1.2754107369177477E-4
12-13	0.0	3.5225629876775886E-4	0.0	2.0042168722993176E-4	1.3361445815328785E-4
14-15	0.0	4.31210296767429E-4	0.0	2.307886095374972E-4	1.7612814938387943E-4
16-17	0.0	4.372836812289421E-4	0.0	2.9152245415262805E-4	2.2471522507598412E-4
18-19	0.0	5.283844481516383E-4	0.0	3.7047645215229814E-4	2.5508214738354955E-4
20-21	0.0	5.951916772282823E-4	0.0	4.615772190749944E-4	2.611555318450626E-4
22-23	0.0	6.437787529203869E-4	0.0	8.259802867657794E-4	2.7330230076808877E-4
24-25	0.0	7.16659366458544E-4	0.0	0.0018159419539924121	3.036692230756542E-4
26-27	0.0	7.591730576891355E-4	0.0	0.004646139113057509	3.097426075371673E-4
28-29	0.0	7.652464421506486E-4	0.0	0.019859967189147787	3.1581599199868035E-4
30-31	0.0	8.502738246118317E-4	0.0	0.0735729793667695	3.1581599199868035E-4
32-33	0.0	8.502738246118317E-4	0.0	0.21075858758342705	3.5225629876775886E-4
34-35	0.0	9.778148983036064E-4	0.0	0.4090120765605986	3.6440306769078507E-4
36-37	0.0	0.0011053559719953812	0.0	0.7020953905198355	3.6440306769078507E-4
38-39	0.0	0.0011053559719953812	0.0	1.0895590990109858	3.765498366138112E-4
40-41	0.0	0.0011903833544565645	0.0	1.4865094340310194	3.765498366138112E-4
42-43	0.0	0.0012389704301486691	0.0	1.9707646706853037	3.765498366138112E-4
44-45	0.0	0.0013725848883019572	0.0	2.575637322745238	3.826232210753243E-4
46-47	0.0	0.0014454655018401141	0.0	3.252406700060564	3.9476998999835044E-4
48-49	0.0	0.0014454655018401141	0.0	3.9178249218173216	4.372836812289421E-4
50-51	0.0	0.0016094468823009674	0.0	4.56730658274707	4.372836812289421E-4
52-53	0.0	0.0016276670356855066	0.0	5.20748989206624	4.7979737245953366E-4
54-55	0.0	0.0016337404201470197	0.0	5.880074707487584	5.405312170746645E-4
56-57	0.0	0.001651960573531559	0.0	6.612682781542062	5.648247549207169E-4
58-59	0.0	0.0016823274958391243	0.0	7.365187263092455	5.76971523843743E-4
60-61	0.0	0.0017187678026082027	0.0	8.093179564956184	6.073384461513084E-4
62-63	0.0	0.0017369879559927422	0.0	8.788077994889125	6.802190596894654E-4
64-65	0.0	0.001943483027684187	0.0	8.968979824459755	6.802190596894654E-4
66-67	0.0	0.0019617031810687264	0.0	8.972891084052968	6.923658286124916E-4
68-69	0.0	0.002010290256760831	0.0	8.9767051694948	7.045125975355177E-4
70-71	0.0	0.0020406571790683964	0.0	8.980057677717554	7.348795198430832E-4
72-73	0.0	0.0020588773324529356	0.0	8.982960755490158	7.773932110736747E-4
74-75	0.0	0.0021196111770680664	0.0	8.98545691650384	7.773932110736747E-4
76-77	0.0	0.0022107119439907625	0.0	8.987813389674905	7.834665955351878E-4
78-79	0.0	0.002247152250759841	0.0	8.98955645101536	7.895399799967009E-4
80-81	0.0	0.002253225635221354	0.0	8.990339917610896	8.381270556888056E-4
82-83	0.0	0.00226537240414438	0.0	8.991032283439509	9.110076692269626E-4
84-85	0.0	0.00228359255752892	0.0	8.991232705126738	9.110076692269626E-4
86-87	0.0	0.002295739326451946	0.0	8.991269145433508	9.292278226115018E-4
88	0.0	0.0024779408602973382	0.0	8.991269145433508	9.353012070730149E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6160	0.0	36.88309	1
GTATCAA	9865	0.0	29.89117	1
ATCAACG	10000	0.0	27.77375	3
TCAACGC	10245	0.0	27.152464	4
CAACGCA	10215	0.0	27.146164	5
AACGCAG	10895	0.0	25.532536	6
TCGGTGG	3785	0.0	24.756475	94
TATCAAC	11605	0.0	24.689934	2
ACGCAGA	13220	0.0	20.975647	7
GTACATG	22680	0.0	20.945951	1
TACATGG	22615	0.0	20.539783	2
CGCAGAG	13485	0.0	20.498268	8
ACATGGG	22890	0.0	19.429077	3
CATGGGG	17670	0.0	18.006094	4
CTCGGTG	4970	0.0	16.295048	94
GTACTAG	2240	0.0	15.891118	1
TCTCGGT	5170	0.0	15.83902	92-93
GCAGAGT	17485	0.0	15.607862	9
ACCGAAC	830	0.0	15.35456	8
AGAGTAC	14310	0.0	15.247445	10-11
>>END_MODULE