FastQCFastQC Report
Fri 27 May 2016
SRR2139263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6881182
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82450.047.196451
GTATCAA147800.036.45921
ATCAACG173050.030.1128063
TCAACGC175800.029.7139364
CAACGCA176450.029.6044795
CGAGACC46200.028.87258786-87
TATCAAC183900.028.819172
AACGCAG183100.028.6447776
AGACCGA47500.028.5656288-89
CCCACGA46950.027.8936382-83
GACCGAG47200.027.85542590-91
ACGAGAC46900.027.71702686-87
GAGACCG47100.027.64362788-89
ACCGAGG50100.027.60554190-91
CACGAGA49900.026.90934684-85
TCCGAGC47550.026.87853876-77
GCCCACG49150.026.14234782-83
TCTCCGA49450.025.58432274-75
ATCTCCG48250.025.13935374-75
ACGCAGA208900.025.0057957
Produced by FastQC (version 0.11.5)