##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139263_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6881182
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.123629632234696	33.0	31.0	34.0	30.0	34.0
2	32.34755569609989	34.0	31.0	34.0	30.0	34.0
3	32.39576049579854	34.0	31.0	34.0	30.0	34.0
4	36.042879987769545	37.0	35.0	37.0	35.0	37.0
5	35.89779168753275	37.0	35.0	37.0	35.0	37.0
6	35.92043082133273	37.0	35.0	37.0	35.0	37.0
7	35.87415112694302	37.0	35.0	37.0	35.0	37.0
8	35.875463110843455	37.0	35.0	37.0	35.0	37.0
9	37.64848364714086	39.0	37.0	39.0	35.0	39.0
10-11	37.52808093144463	39.0	37.0	39.0	35.0	39.0
12-13	37.492761926657366	39.0	37.0	39.0	34.5	39.0
14-15	38.75270890960303	40.0	38.0	41.0	34.5	41.0
16-17	38.73733727722941	40.0	38.0	41.0	34.0	41.0
18-19	38.7211293059826	40.0	38.0	41.0	34.0	41.0
20-21	38.71260139815766	40.0	38.0	41.0	34.0	41.0
22-23	38.635687920232925	40.0	38.0	41.0	34.0	41.0
24-25	38.570588700990925	40.0	38.0	41.0	34.0	41.0
26-27	38.39999238812504	40.0	38.0	41.0	34.0	41.0
28-29	38.265607624590146	40.0	38.0	41.0	34.0	41.0
30-31	38.133981982038954	40.0	38.0	41.0	33.5	41.0
32-33	38.04978730224228	40.0	37.5	41.0	33.0	41.0
34-35	38.10961359439534	40.0	38.0	41.0	33.0	41.0
36-37	38.070080069843456	40.0	37.0	41.0	33.0	41.0
38-39	38.006708660378536	40.0	37.0	41.0	33.0	41.0
40-41	37.93846772353021	40.0	37.0	41.0	33.0	41.0
42-43	37.909915962319275	40.0	37.0	41.0	33.0	41.0
44-45	37.94241816947873	40.0	37.0	41.0	33.0	41.0
46-47	37.931138250888566	40.0	37.0	41.0	33.0	41.0
48-49	37.849801078386534	40.0	37.0	41.0	33.0	41.0
50-51	37.768041100551955	40.0	37.0	41.0	33.0	41.0
52-53	37.542601566612746	40.0	36.5	41.0	32.0	41.0
54-55	37.42524963697744	39.0	36.0	41.0	32.0	41.0
56-57	37.367613416194416	39.0	36.0	41.0	32.0	41.0
58-59	37.21342424136209	39.0	35.5	41.0	31.0	41.0
60-61	37.02871366439578	39.0	35.0	40.5	31.0	41.0
62-63	36.783747471810216	38.5	35.0	40.0	31.0	41.0
64-65	36.502020793991	38.0	35.0	40.0	30.5	41.0
66-67	36.225034369895994	38.0	35.0	40.0	30.0	41.0
68-69	35.95106311012888	37.0	34.0	40.0	30.0	41.0
70-71	35.65776637878696	37.0	34.0	39.0	30.0	41.0
72-73	35.34656222450228	36.0	34.0	39.0	29.0	40.5
74-75	34.993857323426965	36.0	34.0	39.0	29.0	40.0
76-77	33.645880344394776	34.5	32.0	36.5	28.0	39.0
78-79	34.37076040958681	35.0	33.5	37.0	29.0	39.0
80-81	34.30091328810168	35.0	34.0	37.0	29.0	39.0
82-83	34.00722161868867	35.0	33.0	36.0	29.0	38.5
84-85	33.81599373218345	35.0	33.0	36.0	29.0	37.0
86-87	33.573152547995406	35.0	33.0	36.0	29.0	37.0
88-89	33.450455759485635	35.0	33.0	35.0	29.0	36.5
90-91	33.26102450411591	35.0	33.0	35.0	29.0	36.0
92-93	33.14577306401999	35.0	33.0	35.0	29.0	36.0
94-95	33.13752788210162	35.0	33.0	35.0	29.0	36.0
96-97	33.17594621184874	35.0	33.0	35.0	29.0	35.5
98-99	33.280119248311436	35.0	33.0	35.0	29.0	35.0
100	31.965222960025788	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	25.0
21	175.0
22	951.0
23	3407.0
24	9352.0
25	20418.0
26	37852.0
27	60194.0
28	86958.0
29	116759.0
30	148612.0
31	183952.0
32	228914.0
33	287673.0
34	381660.0
35	522156.0
36	796353.0
37	1350251.0
38	1735117.0
39	877973.0
40	32426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.78374907101716	20.914139460342714	14.700860985801567	23.60125048283856
2	14.256402461088808	21.74415674516384	41.77638376662614	22.22305702712121
3	17.744945563131452	26.936258915982748	31.030105002309195	24.288690518576605
4	12.098560973972203	18.942050944154655	40.14824778649947	28.811140295373672
5	12.699576904084212	38.38699514124173	36.14944351130372	12.76398444337034
6	28.567257776352957	39.23206797901872	19.50502108504033	12.69565315958799
7	25.632718332402778	33.1977849154404	23.895691176312443	17.273805575844385
8	23.886303254295555	37.04405725644228	21.68852676763963	17.381112721622536
9	25.298662351904078	17.118192775601635	21.71823096671473	35.86491390577956
10-11	23.556570658936213	27.131254485057944	29.657259465016327	19.654915390989515
12-13	24.60867188224349	24.724545870171724	29.55689153404168	21.10989071354311
14-15	21.675934454284164	26.180734647041742	27.223709531298546	24.919621367375548
16-17	21.173309585190562	29.293668838824377	27.867072696739815	21.665948879245242
18-19	21.15807787561955	28.568880212079968	29.511218863979394	20.761823048321087
20-21	22.119187040572548	27.723851374142328	29.275511943569693	20.881449641715434
22-23	21.808978466999182	27.642845359056555	29.13563486705122	21.412541306893047
24-25	21.55624810273218	27.782942966239393	29.221173741464472	21.43963518956395
26-27	21.532600048044618	27.97883360379947	29.037837064161092	21.45072928399482
28-29	21.411963024205296	28.13840629423286	29.088604116888405	21.36102656467344
30-31	21.757528110888696	27.91320615384974	28.995073560792207	21.334192174469358
32-33	21.571254593388936	28.081085009700207	28.89616810994945	21.451492286961408
34-35	21.624817545085744	28.112584340880453	28.95045055899374	21.312147555040063
36-37	21.61511483741825	28.01856392309673	28.94163521283778	21.42468602664724
38-39	21.51376613272125	28.143401637965116	28.98437953575334	21.3584526935603
40-41	21.733323107326164	27.97067121575063	28.88923968395681	21.406765992966392
42-43	21.642266425904822	28.054448363125513	28.689471230099787	21.613813980869875
44-45	21.758350653153286	28.2592561171702	28.42717316850803	21.555220061168487
46-47	21.76538121950968	28.161459202904837	28.26137404300174	21.81178553458375
48-49	21.792336472112154	28.201311822017104	28.30462056328332	21.701731142587427
50-51	21.72079240245253	28.398220777852597	28.11865340196717	21.762333417727703
52-53	21.82852941393276	28.311417063558096	28.025783840643886	21.834269681865255
54-55	21.860763312299568	28.227078703110585	28.071840692702004	21.840317291887846
56-57	21.866568780105737	28.26664120680996	28.07490330222098	21.79188671086332
58-59	21.77427768944089	28.281108094494677	28.034018958134094	21.910595257930336
60-61	21.96470546954296	28.24077450305216	28.158687618859428	21.635832408545443
62-63	21.7360003857804	28.22709018848979	28.126549027034574	21.910360398695232
64-65	21.680395737121152	28.20135014876865	28.096838279223967	22.02141583488623
66-67	21.781882280108395	28.247670702040672	28.140932430883066	21.82951458696787
68-69	21.686763280933967	28.419607831279155	28.053364969163802	21.840263918623076
70-71	21.70970919816316	28.21176373714295	27.908699606919008	22.169827457774886
72-73	21.790564826414588	28.25224091034334	27.881143481055158	22.07605078218691
74-75	21.70474001011945	28.381627258683732	28.007422548796185	21.90621018240063
76-77	21.752552779551166	28.152358914527586	27.94432723588935	22.150761070031898
78-79	21.75979346855281	28.14635758750466	27.701717658870955	22.39213128507157
80-81	21.940172590610967	28.33719013596405	27.791264017218737	21.93137325620624
82-83	22.008716192153795	28.096937507294555	27.903912401697532	21.99043389885412
84-85	21.949613902589963	28.128594772801186	27.7753205977591	22.146470726849753
86-87	21.706435617527468	28.410461737116993	27.82666259539261	22.056440049962934
88-89	22.017007210938363	28.33411930859549	27.769978734392048	21.878894746074096
90-91	22.034334130860454	28.329240470450124	27.867637091774355	21.76878830691507
92-93	21.81256513012989	28.145711697500236	27.883123747806216	22.158599424563658
94-95	21.779359543565853	28.4671518365874	27.922558238890126	21.830930380956623
96-97	22.029732894045043	28.38236999987972	28.067561674767244	21.520335431307995
98-99	22.714735081551236	30.545546229316955	28.360170950398857	18.379547738732953
100	21.831016419539566	31.644354271112057	22.03749612012964	24.487133189218735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1518.0
1	1363.0
2	1795.5
3	2520.5
4	3289.5
5	5065.5
6	6584.0
7	7643.5
8	8363.0
9	8350.5
10	7863.0
11	7364.0
12	8053.5
13	10631.5
14	16331.0
15	24363.5
16	31158.0
17	35296.0
18	36617.0
19	35604.5
20	33330.0
21	31356.0
22	31319.5
23	33861.0
24	40082.0
25	50269.5
26	64221.5
27	80249.5
28	96499.0
29	114347.0
30	131064.0
31	146177.5
32	163191.0
33	180018.0
34	196958.0
35	213230.0
36	229343.0
37	245852.0
38	256113.0
39	261302.5
40	269308.5
41	277086.5
42	282485.0
43	288556.0
44	292457.0
45	295464.0
46	296576.0
47	295264.0
48	291800.0
49	283416.0
50	273230.5
51	260797.0
52	244770.5
53	227473.0
54	208951.0
55	189297.0
56	170803.5
57	152254.5
58	133300.5
59	114830.0
60	94983.5
61	75621.0
62	59137.0
63	45716.5
64	34391.5
65	25550.0
66	19385.5
67	14976.0
68	11612.5
69	8451.0
70	5966.5
71	4229.5
72	2963.0
73	2057.0
74	1406.5
75	942.5
76	639.5
77	447.5
78	299.5
79	175.0
80	109.5
81	75.0
82	59.5
83	42.5
84	30.0
85	23.0
86	15.5
87	12.5
88	11.0
89	8.0
90	6.0
91	6.0
92	6.0
93	3.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0011989219294010825
18-19	5.8129548092173696E-5
20-21	0.0
22-23	0.0066856918201287285
24-25	0.008518806355320286
26-27	0.020730670703730286
28-29	0.016493415474956162
30-31	0.0017481477100321171
32-33	0.006278292926313063
34-35	0.03245030954827661
36-37	0.005528960503958868
38-39	0.0027585864145313753
40-41	0.007460457342415336
42-43	0.007485740714043655
44-45	0.00870991942529969
46-47	0.005417236469783358
48-49	0.0011955101882149537
50-51	0.002458632343744189
52-53	0.0010351195680728348
54-55	0.005634785896312669
56-57	0.0010758692645110655
58-59	0.0014847439350417306
60-61	9.540194943373283E-4
62-63	0.001542118688308113
64-65	3.5405298032329974E-4
66-67	0.00608565661449768
68-69	0.0044431315695248885
70-71	0.0018930008035491302
72-73	0.0016470072334498936
74-75	6.241184868143267E-4
76-77	5.869884461399552E-4
78-79	8.85693778107492E-4
80-81	0.001066303084196546
82-83	0.006800985834634664
84-85	7.524419954394674E-4
86-87	7.43384850522962E-5
88-89	4.710177147878461E-4
90-91	2.582844745202366E-4
92-93	0.0023835685153364636
94-95	0.0034716611769784428
96-97	4.0772982354064835E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	113.0
20-21	470.0
22-23	1136.0
24-25	2412.0
26-27	3410.0
28-29	5910.0
30-31	14485.0
32-33	18680.0
34-35	25377.0
36-37	37570.0
38-39	44703.0
40-41	47248.0
42-43	56818.0
44-45	63019.0
46-47	62553.0
48-49	57534.0
50-51	51608.0
52-53	48613.0
54-55	51852.0
56-57	55171.0
58-59	33620.0
60-61	57209.0
62-63	55111.0
64-65	57590.0
66-67	60530.0
68-69	62563.0
70-71	62068.0
72-73	60536.0
74-75	61496.0
76-77	60273.0
78-79	64885.0
80-81	66379.0
82-83	65042.0
84-85	66793.0
86-87	71347.0
88-89	79161.0
90-91	85562.0
92-93	94230.0
94-95	117432.0
96-97	289639.0
98-99	482415.0
100-101	4178619.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.59508679797231
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.3015005033996	78.1966627407903
2	6.9747738185180905	12.079622884215977
3	1.627716135405001	4.228566600835684
4	0.5479738943390762	1.8980738777326085
5	0.22668234297368436	0.9814788582686964
6	0.11430806017565959	0.5939109835571451
7	0.06950570653084993	0.4213196882995339
8	0.039996192728192374	0.27707790247089986
9	0.022055657157556217	0.17189203913504103
>10	0.07399274722627425	0.9641259557422291
>50	9.057423686371209E-4	0.05299815621301549
>100	5.646437998055526E-4	0.10658490956913869
>500	0.0	0.0
>1k	2.455537756322559E-5	0.027685403169807412
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4532387023043424E-5	0.0
2	0.0	5.8129548092173696E-5	0.0	1.4532387023043424E-5	0.0
3	0.0	5.8129548092173696E-5	0.0	1.4532387023043424E-5	0.0
4	0.0	7.266193511521713E-5	0.0	1.4532387023043424E-5	0.0
5	0.0	7.266193511521713E-5	0.0	1.4532387023043424E-5	0.0
6	0.0	7.266193511521713E-5	0.0	2.9064774046086848E-5	1.4532387023043424E-5
7	0.0	7.266193511521713E-5	0.0	2.9064774046086848E-5	2.9064774046086848E-5
8	0.0	7.266193511521713E-5	0.0	2.9064774046086848E-5	2.9064774046086848E-5
9	0.0	1.5985625725347768E-4	0.0	8.719432213826054E-5	4.359716106913027E-5
10-11	0.0	1.5985625725347768E-4	0.0	1.3079148320739082E-4	4.359716106913027E-5
12-13	0.0	1.5985625725347768E-4	0.0	1.671224507649994E-4	5.086335458065198E-5
14-15	0.0	2.397843858802165E-4	0.0	2.0345341832260792E-4	9.446051564978226E-5
16-17	0.0	2.470505793917382E-4	0.0	2.3251819236869478E-4	1.3079148320739082E-4
18-19	0.0	2.761153534378251E-4	0.0	2.3251819236869478E-4	1.3079148320739082E-4
20-21	0.0	2.906477404608685E-4	0.0	2.3251819236869478E-4	1.4532387023043425E-4
22-23	7.266193511521712E-6	3.705758690876073E-4	0.0	2.3251819236869478E-4	1.671224507649994E-4
24-25	1.4532387023043424E-5	4.069068366452159E-4	0.0	3.0518012748391194E-4	1.8892103129956452E-4
26-27	1.4532387023043424E-5	4.3597161069130276E-4	0.0	3.633096755760856E-4	1.8892103129956452E-4
28-29	1.4532387023043424E-5	4.432378042028245E-4	0.0	8.719432213826054E-4	2.0345341832260795E-4
30-31	1.4532387023043424E-5	4.6503638473738957E-4	0.0	0.002201656633991079	2.1071961183412967E-4
32-33	1.4532387023043424E-5	4.6503638473738957E-4	0.0	0.005282522682876285	2.1798580534565138E-4
34-35	1.4532387023043424E-5	5.158997393180416E-4	0.0	0.009097274276425184	2.470505793917382E-4
36-37	1.4532387023043424E-5	5.667630938986936E-4	0.0	0.01541159643793755	2.470505793917382E-4
38-39	1.4532387023043424E-5	6.030940614563022E-4	0.0	0.033954922279340966	2.5431677290325993E-4
40-41	2.9064774046086848E-5	7.120869641291278E-4	0.0	0.05353731379289198	2.6158296641478165E-4
42-43	2.9064774046086848E-5	7.338855446636929E-4	0.0	0.06666732546821172	2.761153534378251E-4
44-45	2.9064774046086848E-5	7.920150927558667E-4	0.0	0.08286367080539361	2.906477404608685E-4
46-47	2.9064774046086848E-5	8.283460603134752E-4	0.0	0.09996829033151572	3.1971251450695537E-4
48-49	2.9064774046086848E-5	8.356122538249969E-4	0.0	0.11620096663625523	3.560434820645639E-4
50-51	4.359716106913027E-5	9.954685110784745E-4	0.0	0.1324845062955754	3.7784206259912904E-4
52-53	4.359716106913027E-5	0.0010027347045899963	0.0	0.17335684479788502	3.8510825611065075E-4
54-55	4.359716106913027E-5	0.0010172670916130397	0.0	0.22219293138882246	3.9237444962217247E-4
56-57	4.359716106913027E-5	0.0010463318656591266	0.0	0.2711380108824327	4.069068366452159E-4
58-59	4.359716106913027E-5	0.0010535980591706482	0.0	0.4720046643149389	4.3597161069130276E-4
60-61	4.359716106913027E-5	0.0010971952202397787	0.0	0.6519882775953317	4.505039977143462E-4
62-63	4.359716106913027E-5	0.0011335261877973872	0.0	0.6609038970339689	4.505039977143462E-4
64-65	4.359716106913027E-5	0.001264317671004778	0.0	0.6676687231931956	4.505039977143462E-4
66-67	4.359716106913027E-5	0.0013079148320739083	0.0	0.6741429016119614	4.5777019122586785E-4
68-69	4.359716106913027E-5	0.0013297134126084732	0.0	0.6806897419658425	4.6503638473738957E-4
70-71	4.359716106913027E-5	0.001336979606119995	0.0	0.6863137757437603	4.6503638473738957E-4
72-73	4.359716106913027E-5	0.0013515119931430384	0.0	0.6907606861728115	5.086335458065199E-4
74-75	4.359716106913027E-5	0.0013951091542121687	0.0	0.6942121280907845	5.086335458065199E-4
76-77	4.359716106913027E-5	0.0014459725087928206	0.0	0.6974673827839462	5.086335458065199E-4
78-79	4.359716106913027E-5	0.0014677710893273859	0.0	0.6995745789022874	5.086335458065199E-4
80-81	4.359716106913027E-5	0.0014750372828389075	0.0	0.7004029249626009	5.158997393180416E-4
82-83	4.359716106913027E-5	0.0014823034763504293	0.0	0.700976954250011	5.594969003871719E-4
84-85	4.359716106913027E-5	0.0014968358633734727	0.0	0.7011513428942876	5.667630938986936E-4
86-87	4.359716106913027E-5	0.0015259006374195596	0.0	0.7012312710229144	5.667630938986936E-4
88	4.359716106913027E-5	0.0015694977984886899	0.0	0.7012312710229144	5.81295480921737E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8245	0.0	47.19645	1
GTATCAA	14780	0.0	36.4592	1
ATCAACG	17305	0.0	30.112806	3
TCAACGC	17580	0.0	29.713936	4
CAACGCA	17645	0.0	29.604479	5
CGAGACC	4620	0.0	28.872587	86-87
TATCAAC	18390	0.0	28.81917	2
AACGCAG	18310	0.0	28.644777	6
AGACCGA	4750	0.0	28.56562	88-89
CCCACGA	4695	0.0	27.89363	82-83
GACCGAG	4720	0.0	27.855425	90-91
ACGAGAC	4690	0.0	27.717026	86-87
GAGACCG	4710	0.0	27.643627	88-89
ACCGAGG	5010	0.0	27.605541	90-91
CACGAGA	4990	0.0	26.909346	84-85
TCCGAGC	4755	0.0	26.878538	76-77
GCCCACG	4915	0.0	26.142347	82-83
TCTCCGA	4945	0.0	25.584322	74-75
ATCTCCG	4825	0.0	25.139353	74-75
ACGCAGA	20890	0.0	25.005795	7
>>END_MODULE