FastQCFastQC Report
Fri 27 May 2016
SRR2139262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6174646
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72950.048.047991
GTATCAA126900.036.55031
TCAACGC152250.029.825864
ATCAACG152050.029.8094753
CAACGCA156000.029.0275825
TATCAAC162350.028.17872
AACGCAG162200.028.100496
AGACCGA49400.025.7591588-89
ACCGAGG51400.025.44069990-91
CGAGACC49700.025.31734386-87
GACCGAG50350.025.2613290-91
GAGACCG50150.024.68032588-89
ACGCAGA184300.024.5932227
ACGAGAC50300.024.50713386-87
CGCAGAG187050.024.276868
CCCACGA51600.023.58727882-83
GCCCACG50950.023.18718582-83
CACGAGA54000.023.0581284-85
TCTCCGA52200.022.35107674-75
TCCGAGC52450.022.1743176-77
Produced by FastQC (version 0.11.5)