##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139262_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6174646
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13711701043266	33.0	31.0	34.0	30.0	34.0
2	32.36222805323577	34.0	31.0	34.0	30.0	34.0
3	32.409469465941854	34.0	31.0	34.0	30.0	34.0
4	36.05446174566121	37.0	35.0	37.0	35.0	37.0
5	35.91011419925936	37.0	35.0	37.0	35.0	37.0
6	35.93070210016898	37.0	35.0	37.0	35.0	37.0
7	35.884899798304225	37.0	35.0	37.0	35.0	37.0
8	35.88238289288163	37.0	35.0	37.0	35.0	37.0
9	37.658011487622126	39.0	37.0	39.0	35.0	39.0
10-11	37.53830689241133	39.0	37.0	39.0	35.0	39.0
12-13	37.504486249090235	39.0	37.0	39.0	35.0	39.0
14-15	38.768965945578095	40.0	38.0	41.0	34.5	41.0
16-17	38.75542160635605	40.0	38.0	41.0	34.0	41.0
18-19	38.733648536288555	40.0	38.0	41.0	34.0	41.0
20-21	38.72509952529272	40.0	38.0	41.0	34.0	41.0
22-23	38.649362660648585	40.0	38.0	41.0	34.0	41.0
24-25	38.58818860698117	40.0	38.0	41.0	34.0	41.0
26-27	38.41778039553836	40.0	38.0	41.0	34.0	41.0
28-29	38.28503097916479	40.0	38.0	41.0	34.0	41.0
30-31	38.15243560631119	40.0	38.0	41.0	33.5	41.0
32-33	38.06467799609637	40.0	37.5	41.0	33.0	41.0
34-35	38.11951663180944	40.0	38.0	41.0	33.0	41.0
36-37	38.08245885482638	40.0	37.5	41.0	33.0	41.0
38-39	38.01841408888181	40.0	37.0	41.0	33.0	41.0
40-41	37.94950460938489	40.0	37.0	41.0	33.0	41.0
42-43	37.92149316125078	40.0	37.0	41.0	33.0	41.0
44-45	37.95220538324129	40.0	37.0	41.0	33.0	41.0
46-47	37.93863400251763	40.0	37.0	41.0	33.0	41.0
48-49	37.8581857055663	40.0	37.0	41.0	33.0	41.0
50-51	37.77387079281789	40.0	37.0	41.0	33.0	41.0
52-53	37.54459890587742	40.0	36.0	41.0	32.0	41.0
54-55	37.425147981619546	39.0	36.0	41.0	32.0	41.0
56-57	37.35900051563904	39.0	36.0	41.0	32.0	41.0
58-59	37.20609444663074	39.0	35.5	41.0	31.0	41.0
60-61	37.017359871684114	39.0	35.0	40.5	31.0	41.0
62-63	36.769797594423665	38.5	35.0	40.0	31.0	41.0
64-65	36.48320078816245	38.0	35.0	40.0	30.5	41.0
66-67	36.20677783888689	37.5	35.0	40.0	30.0	41.0
68-69	35.931469084235275	37.0	34.0	40.0	30.0	41.0
70-71	35.63246162751031	36.5	34.0	39.0	30.0	41.0
72-73	35.32461643941909	36.0	34.0	39.0	29.5	40.5
74-75	34.96846425903728	36.0	34.0	39.0	29.0	40.0
76-77	33.630178788354954	34.5	32.0	36.5	28.0	39.0
78-79	34.344747232870326	35.0	33.5	37.0	29.0	39.0
80-81	34.28249866259531	35.0	34.0	37.0	29.0	39.0
82-83	33.99554622699053	35.0	33.5	36.0	29.0	38.5
84-85	33.80831986075525	35.0	33.0	36.0	29.0	37.0
86-87	33.57018297877245	35.0	33.0	35.5	29.0	37.0
88-89	33.44726572059338	35.0	33.0	35.0	29.0	36.5
90-91	33.26354709274548	35.0	33.0	35.0	29.0	36.0
92-93	33.15461809301717	35.0	33.0	35.0	29.0	36.0
94-95	33.14669674397932	35.0	33.0	35.0	29.0	36.0
96-97	33.18542159641108	35.0	33.0	35.0	29.0	35.0
98-99	33.29122382221832	35.0	33.0	35.0	29.0	35.0
100	31.994187957591603	34.0	31.0	35.0	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	26.0
21	161.0
22	875.0
23	3083.0
24	8504.0
25	18334.0
26	33817.0
27	53928.0
28	77451.0
29	104903.0
30	132718.0
31	164874.0
32	204251.0
33	257274.0
34	340976.0
35	468127.0
36	715266.0
37	1214731.0
38	1553166.0
39	791784.0
40	30394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.89905720910964	20.725965504743105	14.651204295760436	23.72377299038682
2	14.427288625129279	21.620818424246508	41.597947477474825	22.35394547314939
3	17.818786696435716	26.747832993178882	30.924590656695138	24.50878965369027
4	12.287732770429269	18.80120738905518	39.72494941410407	29.18611042641149
5	12.861935728785099	38.14366038150203	36.09104068476152	12.903363204951345
6	28.929059252951504	38.892642590360644	19.326128169938812	12.852169986749038
7	25.778660023586774	32.984643977970556	23.811211201419482	17.42548479702318
8	23.965292909099567	36.94504915747397	21.656269849316057	17.43338808411041
9	25.317240858828182	17.072055628776127	21.573123382295925	36.03758013009977
10-11	23.705537127148666	26.983757449414913	29.48200593199999	19.82869949143643
12-13	24.762569384544474	24.57676925932272	29.37434793832715	21.28631341780565
14-15	21.839470635239657	25.993724984395865	27.030958535922544	25.135845844441935
16-17	21.388087347424374	29.15050964756847	27.639155237109165	21.82224776789799
18-19	21.395457307852237	28.39510141680267	29.24599278419763	20.963448491147464
20-21	22.2377143527184	27.524200088072465	29.128089075305517	21.109996483903615
22-23	21.97357426595701	27.49614465093146	28.922540791055024	21.60774029205651
24-25	21.78392029816254	27.611951905672594	28.998968601815495	21.605159194349376
26-27	21.747392385632317	27.76936365658877	28.88867546279355	21.594568494985364
28-29	21.608598983231403	27.884026205155553	28.894178404220288	21.613196407392753
30-31	21.964771937548306	27.65971400737879	28.84438033281662	21.531133722256286
32-33	21.80324948075182	27.821272863801184	28.75003762667988	21.625440028767123
34-35	21.83881007896736	27.84688025918865	28.81793987171324	21.49636979013075
36-37	21.85232715136528	27.844545356288396	28.733265916129252	21.569861576217072
38-39	21.67855477015871	27.916328763957317	28.814738539338798	21.59037792654518
40-41	21.917892901728464	27.775318415909116	28.705488258471696	21.601300423890727
42-43	21.8300321659352	27.850953940684388	28.531885800774482	21.787128092605933
44-45	21.92432351683066	28.00822501560042	28.303920581777124	21.763530885791795
46-47	21.956499073782883	27.94927062264004	28.086664814090582	22.00756548948649
48-49	21.95017267375815	27.989282620623996	28.132321903850283	21.928222801767568
50-51	21.93241919762974	28.113819696651017	28.010283745011588	21.94347736070766
52-53	22.03419199496798	28.05420636543305	27.92830103879755	21.98330060080142
54-55	21.982037866001384	27.968441859730316	27.99745080383203	22.05206947043627
56-57	22.010333273130605	28.01683783027002	27.939580128129748	22.033248768469626
58-59	21.90821732801409	28.051528996055623	27.93767804854327	22.102575627387015
60-61	22.16244954513162	28.008765565173782	28.004640550943382	21.82414433875121
62-63	21.908749570268917	28.06774361684586	27.949227829375804	22.074278983509416
64-65	21.89062942082451	27.995280050230658	27.978243534288993	22.135846994655836
66-67	21.921453932599057	28.023175358862513	28.037176721192292	22.01819398734614
68-69	21.872977406885806	28.233733107601957	27.866454544092527	22.02683494141971
70-71	21.89488808320512	28.025572598380627	27.728096399363384	22.351442919050864
72-73	21.94200701871466	28.041165591843875	27.70138434216674	22.315443047274726
74-75	21.907044319421264	28.105021016694053	27.89983296083542	22.08810170304926
76-77	21.999235855011793	27.835857356996424	27.826027051905182	22.3388797360866
78-79	21.94464195860808	27.866687967568883	27.610178076925703	22.57849199689734
80-81	22.156591562169346	28.09973394137592	27.634123850684432	22.1095506457703
82-83	22.205610261333742	27.85165950756602	27.72944823965675	22.21328199144349
84-85	22.10678163966831	27.833065692204368	27.655660394753934	22.40449227337339
86-87	21.925296088900907	28.027947434785013	27.76353185753785	22.283224618776234
88-89	22.24799564739387	27.956340523880463	27.72520038791308	22.070463440812585
90-91	22.269258728699405	27.928908939299994	27.779008359881125	22.022823972119475
92-93	22.043129529235276	27.69039607683556	27.81474898106653	22.45172541286263
94-95	22.07439315533703	27.971040786241876	27.906293674479045	22.04827238394205
96-97	22.31279041890774	27.90262729469483	27.96522400124851	21.81935828514892
98-99	22.973479462168537	30.047920665025572	28.354719648728278	18.623880224077606
100	22.02976339609793	31.081029716285176	22.03070026103734	24.858506626579555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	978.0
1	892.5
2	1296.0
3	1894.5
4	2551.0
5	4115.0
6	5504.5
7	6535.5
8	7303.5
9	7395.0
10	6987.0
11	6439.5
12	6920.5
13	9037.5
14	13909.5
15	20822.0
16	26660.0
17	30425.0
18	31984.5
19	31350.0
20	29513.5
21	27828.0
22	27691.0
23	29833.0
24	34907.0
25	43121.0
26	54235.5
27	67134.0
28	81429.5
29	97387.5
30	112951.0
31	128120.0
32	143158.5
33	156984.0
34	171412.5
35	185477.5
36	199272.0
37	213005.0
38	223585.5
39	231161.5
40	239217.5
41	246418.0
42	251331.0
43	255882.0
44	260067.5
45	263542.5
46	264309.5
47	263090.5
48	259496.0
49	252256.5
50	245054.0
51	235649.5
52	221972.5
53	207823.0
54	193473.0
55	177190.0
56	160685.5
57	144133.0
58	127120.5
59	110506.0
60	92573.0
61	75580.0
62	60610.0
63	47457.0
64	36504.5
65	27640.5
66	21222.0
67	16588.5
68	12691.5
69	9169.5
70	6596.0
71	4867.5
72	3479.0
73	2454.0
74	1741.5
75	1171.0
76	779.0
77	508.0
78	318.0
79	202.0
80	144.0
81	100.0
82	64.0
83	39.0
84	28.0
85	24.0
86	18.5
87	12.5
88	9.5
89	8.0
90	6.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0013765971360949277
18-19	5.668341148626172E-5
20-21	0.0
22-23	0.00681893295361109
24-25	0.008156874434759787
26-27	0.02095459101232796
28-29	0.016538261389329078
30-31	0.0020697324517734077
32-33	0.006133789500709534
34-35	0.033502294450246196
36-37	0.005547230612715793
38-39	0.0024808909580014008
40-41	0.0070359996757466195
42-43	0.007622351911785318
44-45	0.008353808312384888
46-47	0.00533615568025687
48-49	8.590874137766178E-4
50-51	0.0027471327560137076
52-53	9.519665620435036E-4
54-55	0.00530687651505604
56-57	0.0010383753348760455
58-59	0.0012872428866421734
60-61	8.195921799377705E-4
62-63	0.001608909102362342
64-65	4.3118621253645476E-4
66-67	0.005420485097279639
68-69	0.0040824749836045554
70-71	0.0017793926081191601
72-73	0.0015210427656957982
74-75	7.250750380156843E-4
76-77	6.546979414244683E-4
78-79	8.397780535762591E-4
80-81	0.0011297690752010289
82-83	0.0072866005185023485
84-85	8.092681071434096E-4
86-87	9.337798599454714E-5
88-89	4.945162358097515E-4
90-91	3.2071052347440676E-4
92-93	0.002556174379388054
94-95	0.0033065983391777644
96-97	3.419960944046019E-5
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	111.0
20-21	354.0
22-23	938.0
24-25	2149.0
26-27	2449.0
28-29	5417.0
30-31	13033.0
32-33	17156.0
34-35	23116.0
36-37	33805.0
38-39	39662.0
40-41	42121.0
42-43	49393.0
44-45	55673.0
46-47	55985.0
48-49	51641.0
50-51	45955.0
52-53	43090.0
54-55	46350.0
56-57	50491.0
58-59	31101.0
60-61	51657.0
62-63	50020.0
64-65	52727.0
66-67	56273.0
68-69	56919.0
70-71	56767.0
72-73	54711.0
74-75	55406.0
76-77	54602.0
78-79	59333.0
80-81	60771.0
82-83	59786.0
84-85	60663.0
86-87	65297.0
88-89	72894.0
90-91	78936.0
92-93	87509.0
94-95	104247.0
96-97	258305.0
98-99	431969.0
100-101	3735864.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.34383787839329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64374607593797	79.17172661946975
2	6.842465270306317	11.952943545163428
3	1.5127798887304356	3.96396004040895
4	0.49627166010575136	1.733850856956714
5	0.2209441185007583	0.9649053633257373
6	0.10326613594400327	0.5411796381732686
7	0.05904746448854687	0.3610202515792476
8	0.038999933696689124	0.27251231088573624
9	0.021757271257958975	0.17103272160882554
>10	0.05955325548916202	0.7607690521268471
>50	8.14910274631418E-4	0.04666325903519869
>100	3.5401526776912746E-4	0.059436341266326465
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6195260424646207E-5
2	0.0	3.2390520849292413E-5	0.0	1.6195260424646207E-5	1.6195260424646207E-5
3	0.0	3.2390520849292413E-5	0.0	1.6195260424646207E-5	1.6195260424646207E-5
4	0.0	1.1336682297252345E-4	0.0	1.6195260424646207E-5	1.6195260424646207E-5
5	0.0	1.1336682297252345E-4	0.0	1.6195260424646207E-5	1.6195260424646207E-5
6	9.717156254787724E-5	1.1336682297252345E-4	0.0	1.6195260424646207E-5	4.858578127393862E-5
7	9.717156254787724E-5	1.1336682297252345E-4	0.0	1.6195260424646207E-5	4.858578127393862E-5
8	9.717156254787724E-5	1.2956208339716965E-4	0.0	1.6195260424646207E-5	4.858578127393862E-5
9	9.717156254787724E-5	2.1053838552040067E-4	0.0	3.2390520849292413E-5	6.478104169858483E-5
10-11	1.2956208339716965E-4	2.1053838552040067E-4	0.0	6.478104169858483E-5	6.478104169858483E-5
12-13	1.2956208339716965E-4	2.1053838552040067E-4	0.0	7.287867191090792E-5	6.478104169858483E-5
14-15	1.2956208339716965E-4	3.0770994806827794E-4	0.0	8.097630212323103E-5	6.478104169858483E-5
16-17	2.267336459450469E-4	3.0770994806827794E-4	0.0	1.0526919276020034E-4	6.478104169858483E-5
18-19	2.267336459450469E-4	3.562957293422166E-4	0.0	1.5385497403413897E-4	6.478104169858483E-5
20-21	2.3483127615737E-4	4.2107677104080135E-4	0.0	2.186360157327238E-4	6.478104169858483E-5
22-23	2.510265365820162E-4	4.4536966167777066E-4	0.0	5.263459638010017E-4	1.1336682297252343E-4
24-25	2.591241667943393E-4	5.020530731640325E-4	0.0	0.0015466473705537127	1.4575734382181585E-4
26-27	2.591241667943393E-4	5.344435940133249E-4	0.0	0.0038949601321274126	1.4575734382181585E-4
28-29	2.753194272189855E-4	5.344435940133249E-4	0.0	0.012640400761436365	1.4575734382181585E-4
30-31	2.753194272189855E-4	7.0449382847211E-4	0.0	0.013887435814134121	1.4575734382181585E-4
32-33	2.753194272189855E-4	7.206890888967561E-4	0.0	0.016948340034392256	1.4575734382181585E-4
34-35	2.753194272189855E-4	8.502511722939259E-4	0.0	0.020713738083122497	1.6195260424646207E-4
36-37	2.753194272189855E-4	9.717156254787723E-4	0.0	0.027629114284446428	1.6195260424646207E-4
38-39	2.753194272189855E-4	9.717156254787723E-4	0.0	0.04667474054383037	1.6195260424646207E-4
40-41	2.753194272189855E-4	0.001012203776540388	0.0	0.0672589165435557	1.6195260424646207E-4
42-43	3.0770994806827794E-4	0.0010364966671773572	0.0	0.08070908032622437	1.7005023445878516E-4
44-45	3.2390520849292413E-4	0.0011174729693005881	0.0	0.09679907155811038	1.7814786467110826E-4
46-47	3.4010046891757033E-4	0.0011660587505745268	0.0	0.11390126656653676	1.8624549488343136E-4
48-49	3.481980991298934E-4	0.0011660587505745268	0.0	0.1295053999856834	2.1053838552040067E-4
50-51	3.7249098976686277E-4	0.0012713279433347272	0.0	0.1449961665818575	2.1053838552040067E-4
52-53	3.7249098976686277E-4	0.0012794255735470504	0.0	0.18532236503922656	2.2673364594504687E-4
54-55	3.7249098976686277E-4	0.0012875232037593736	0.0	0.23246676813537165	2.429289063696931E-4
56-57	3.7249098976686277E-4	0.0013118160943963427	0.0	0.279141508679202	2.510265365820162E-4
58-59	3.7249098976686277E-4	0.0013118160943963427	0.0	0.4773229104955976	2.591241667943393E-4
60-61	3.805886199791859E-4	0.0013361089850333122	0.0	0.6562805381879382	2.753194272189855E-4
62-63	3.8868625019150896E-4	0.0013604018756702813	0.0	0.6651231503797951	2.753194272189855E-4
64-65	4.2917440125312447E-4	0.0016519165633139131	0.0	0.672289553117701	2.753194272189855E-4
66-67	4.534672918900938E-4	0.0016519165633139131	0.0	0.6793182961419975	2.753194272189855E-4
68-69	4.534672918900938E-4	0.0016762094539508822	0.0	0.686274160494383	2.753194272189855E-4
70-71	4.7776018252706305E-4	0.0016924047143755286	0.0	0.6916833774762148	3.15807578280601E-4
72-73	5.344435940133249E-4	0.0017005023445878517	0.0	0.6961451717232049	3.4010046891757033E-4
74-75	5.425412242256479E-4	0.001732892865437144	0.0	0.6994732977404696	3.4010046891757033E-4
76-77	5.587364846502941E-4	0.0018300644279850213	0.0	0.7027366427160359	3.4010046891757033E-4
78-79	5.668341148626172E-4	0.00187865020925896	0.0	0.704769147899329	3.4010046891757033E-4
80-81	5.992246357119096E-4	0.00187865020925896	0.0	0.7053602749048286	3.4010046891757033E-4
82-83	5.992246357119096E-4	0.00187865020925896	0.0	0.7058380350873557	3.4010046891757033E-4
84-85	6.154198961365559E-4	0.00187865020925896	0.0	0.7060809639937253	3.4010046891757033E-4
86-87	6.154198961365559E-4	0.001894845469683606	0.0	0.7062105260771225	3.4010046891757033E-4
88	6.154198961365559E-4	0.0020082122926561295	0.0	0.7062267213375472	3.4010046891757033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7295	0.0	48.04799	1
GTATCAA	12690	0.0	36.5503	1
TCAACGC	15225	0.0	29.82586	4
ATCAACG	15205	0.0	29.809475	3
CAACGCA	15600	0.0	29.027582	5
TATCAAC	16235	0.0	28.1787	2
AACGCAG	16220	0.0	28.10049	6
AGACCGA	4940	0.0	25.75915	88-89
ACCGAGG	5140	0.0	25.440699	90-91
CGAGACC	4970	0.0	25.317343	86-87
GACCGAG	5035	0.0	25.26132	90-91
GAGACCG	5015	0.0	24.680325	88-89
ACGCAGA	18430	0.0	24.593222	7
ACGAGAC	5030	0.0	24.507133	86-87
CGCAGAG	18705	0.0	24.27686	8
CCCACGA	5160	0.0	23.587278	82-83
GCCCACG	5095	0.0	23.187185	82-83
CACGAGA	5400	0.0	23.05812	84-85
TCTCCGA	5220	0.0	22.351076	74-75
TCCGAGC	5245	0.0	22.17431	76-77
>>END_MODULE